antibodies against anti aqp2  (Alomone Labs)


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    Structured Review

    Alomone Labs antibodies against anti aqp2
    <t>AQP2</t> expression in Epac1 −/− and Epac2 −/− mice after water deprivation. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . Intensity values were normalized to total signal of respective lines in Ponceau red staining. Number of mice in each group is indicated on each bar. C ) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation. Nuclear DAPI staining is shown in pseudocolor blue. Number of mice in each group is indicated on each bar. * P
    Antibodies Against Anti Aqp2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Urinary concentrating defect in mice lacking Epac1 or Epac2"

    Article Title: Urinary concentrating defect in mice lacking Epac1 or Epac2

    Journal: The FASEB Journal

    doi: 10.1096/fj.201800435R

    AQP2 expression in Epac1 −/− and Epac2 −/− mice after water deprivation. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . Intensity values were normalized to total signal of respective lines in Ponceau red staining. Number of mice in each group is indicated on each bar. C ) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation. Nuclear DAPI staining is shown in pseudocolor blue. Number of mice in each group is indicated on each bar. * P
    Figure Legend Snippet: AQP2 expression in Epac1 −/− and Epac2 −/− mice after water deprivation. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . Intensity values were normalized to total signal of respective lines in Ponceau red staining. Number of mice in each group is indicated on each bar. C ) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation. Nuclear DAPI staining is shown in pseudocolor blue. Number of mice in each group is indicated on each bar. * P

    Techniques Used: Expressing, Mouse Assay, Western Blot, Staining

    AQP2 expression and localization in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A ). * P
    Figure Legend Snippet: AQP2 expression and localization in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A ). * P

    Techniques Used: Expressing, Mouse Assay, Western Blot

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    Alomone Labs aqp2 4
    Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of <t>AQP2-4,</t> BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.
    Aqp2 4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs antibodies against anti aqp2
    <t>AQP2</t> expression in Epac1 −/− and Epac2 −/− mice after water deprivation. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . Intensity values were normalized to total signal of respective lines in Ponceau red staining. Number of mice in each group is indicated on each bar. C ) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation. Nuclear DAPI staining is shown in pseudocolor blue. Number of mice in each group is indicated on each bar. * P
    Antibodies Against Anti Aqp2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against anti aqp2/product/Alomone Labs
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    antibodies against anti aqp2 - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of AQP2-4, BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.

    Journal: Cells

    Article Title: Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

    doi: 10.3390/cells9041060

    Figure Lengend Snippet: Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of AQP2-4, BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.

    Article Snippet: Antibodies raised against Aqp2-4 were obtained from Alomone Labs (Jerusalem, Israel) and antibodies directed against phosphorylated Aqp2 at position S256 were a generous gift from M. Knepper (National Institutes of Health, Bethesda, MD, USA).

    Techniques: Concentration Assay, Expressing, Real-time Polymerase Chain Reaction

    MG132 and bafilomycin induce downregulation of Aqp2 on the mRNA level. IMCD cells were treated for 1 or 24 h with bafilomycin (Baf. 100 nM) or MG132 (2 µM) and the mRNA expression of Aqp2-4, BGT-1, and AR was compared to untreated cells by real-time PCR. One-way ANOVA with tukey post-test was performed and * indicates statistically significant differences compared untreated cells ( p ≤ 0.05).

    Journal: Cells

    Article Title: Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

    doi: 10.3390/cells9041060

    Figure Lengend Snippet: MG132 and bafilomycin induce downregulation of Aqp2 on the mRNA level. IMCD cells were treated for 1 or 24 h with bafilomycin (Baf. 100 nM) or MG132 (2 µM) and the mRNA expression of Aqp2-4, BGT-1, and AR was compared to untreated cells by real-time PCR. One-way ANOVA with tukey post-test was performed and * indicates statistically significant differences compared untreated cells ( p ≤ 0.05).

    Article Snippet: Antibodies raised against Aqp2-4 were obtained from Alomone Labs (Jerusalem, Israel) and antibodies directed against phosphorylated Aqp2 at position S256 were a generous gift from M. Knepper (National Institutes of Health, Bethesda, MD, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Migration influences the localization of AQPs. Wound healing assays were performed with IMCD-cells cultivated at 600 mosmol/kg. A scratch was induced and after 4 h the cells were stained for AQP2–4 using specific antibodies. For the AQP2 staining the cells were incubated with dbcAMP to induce membrane localization. Alexa-488 labeled secondary antibodies were used for visualization. The nuclei were stained with DAPI. The upper row show cells away from the wound. The lower rows show cells at the leading edge. Scale bar = 20 µm, representative image chosen from 6 to 9 independent experiments.

    Journal: Scientific Reports

    Article Title: Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells

    doi: 10.1038/s41598-021-91369-y

    Figure Lengend Snippet: Migration influences the localization of AQPs. Wound healing assays were performed with IMCD-cells cultivated at 600 mosmol/kg. A scratch was induced and after 4 h the cells were stained for AQP2–4 using specific antibodies. For the AQP2 staining the cells were incubated with dbcAMP to induce membrane localization. Alexa-488 labeled secondary antibodies were used for visualization. The nuclei were stained with DAPI. The upper row show cells away from the wound. The lower rows show cells at the leading edge. Scale bar = 20 µm, representative image chosen from 6 to 9 independent experiments.

    Article Snippet: Antibodies raised against AQP2–4 were obtained from Alomone Labs (Jerusalem, Israel) .

    Techniques: Migration, Staining, Incubation, Labeling

    AQP2 expression in Epac1 −/− and Epac2 −/− mice after water deprivation. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . Intensity values were normalized to total signal of respective lines in Ponceau red staining. Number of mice in each group is indicated on each bar. C ) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation. Nuclear DAPI staining is shown in pseudocolor blue. Number of mice in each group is indicated on each bar. * P

    Journal: The FASEB Journal

    Article Title: Urinary concentrating defect in mice lacking Epac1 or Epac2

    doi: 10.1096/fj.201800435R

    Figure Lengend Snippet: AQP2 expression in Epac1 −/− and Epac2 −/− mice after water deprivation. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− from Western blots similar to that shown in A . Intensity values were normalized to total signal of respective lines in Ponceau red staining. Number of mice in each group is indicated on each bar. C ) Representative confocal micrographs of cortical (top) and medullary (bottom) CDs in transverse cut kidney sections probed with anti-AQP2 (pseudocolor green) from Epac WT, Epac1 −/− , and Epac2 −/− mice subjected to 24 h of water deprivation. Nuclear DAPI staining is shown in pseudocolor blue. Number of mice in each group is indicated on each bar. * P

    Article Snippet: We used the primary antibodies against anti-AQP2 (1:1000, AQP2-002; Alomone Labs, Jerusalem, Israel), anti–Na+ -K+ -Cl− cotransporter type 2 ( SLC12A1 ; NKCC2; rabbit polyclonal, 1:1000, ANT-072; Alomone Labs), anti–NHE-3, pS552 NHE-3 (mouse monoclonal, 1:100, sc-136368, sc-53962; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Na+ /H+ exchanger regulatory factor isoform 1 (NHERF1; mouse monoclonal, 1:1000, sc-271552; Santa Cruz Biotechnology), anti-pT96 T101 NKCC2 (rabbit polyclonal, 1:2000, gift of B. Forbush, Yale University, New Haven, CT, USA), anti-pT53 thiazide-sensitive Na+ -Cl− cotransporter ( SLC12A3 ) (NCC; rabbit polyclonal, 1:1000; p1311-53; PhosphoSolutions, Aurora, CO, USA), anti–α subunit of epithelial Na+ ).

    Techniques: Expressing, Mouse Assay, Western Blot, Staining

    AQP2 expression and localization in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A ). * P

    Journal: The FASEB Journal

    Article Title: Urinary concentrating defect in mice lacking Epac1 or Epac2

    doi: 10.1096/fj.201800435R

    Figure Lengend Snippet: AQP2 expression and localization in Epac1 −/− and Epac2 −/− mice. A ) Representative Western blots from whole kidney lysates of Epac WT, Epac1 −/− , and Epac2 −/− mice probed with anti-AQP2 antibodies. AQP2 is present as upper duplet of glycosylated bands around 37 kDa and lower nonglycosylated band at 29 kDa. B ) Summary graph comparing total renal AQP2 expression (both glycosylated and nonglycosylated forms) in Epac WT, Epac1 −/− , and Epac2 −/− mice from Western blots similar to that shown in A ). * P

    Article Snippet: We used the primary antibodies against anti-AQP2 (1:1000, AQP2-002; Alomone Labs, Jerusalem, Israel), anti–Na+ -K+ -Cl− cotransporter type 2 ( SLC12A1 ; NKCC2; rabbit polyclonal, 1:1000, ANT-072; Alomone Labs), anti–NHE-3, pS552 NHE-3 (mouse monoclonal, 1:100, sc-136368, sc-53962; Santa Cruz Biotechnology, Dallas, TX, USA), anti-Na+ /H+ exchanger regulatory factor isoform 1 (NHERF1; mouse monoclonal, 1:1000, sc-271552; Santa Cruz Biotechnology), anti-pT96 T101 NKCC2 (rabbit polyclonal, 1:2000, gift of B. Forbush, Yale University, New Haven, CT, USA), anti-pT53 thiazide-sensitive Na+ -Cl− cotransporter ( SLC12A3 ) (NCC; rabbit polyclonal, 1:1000; p1311-53; PhosphoSolutions, Aurora, CO, USA), anti–α subunit of epithelial Na+ ).

    Techniques: Expressing, Mouse Assay, Western Blot

    Empagliflozin increases V2R expression but decreases AQP2 expression in diabetic rat kidneys. (A) Representative immunoblot reacting with anti-V2R, anti-AQP2 and anti-p261-AQP2. (B) Densitometric analysis shows that renal expression of V2R protein in the empagliflozin-treated OLETF group was significantly increased compared with untreated OLETF or lixisenatide-treated OLETF rats. ∗ P

    Journal: Frontiers in Physiology

    Article Title: Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys

    doi: 10.3389/fphys.2019.00271

    Figure Lengend Snippet: Empagliflozin increases V2R expression but decreases AQP2 expression in diabetic rat kidneys. (A) Representative immunoblot reacting with anti-V2R, anti-AQP2 and anti-p261-AQP2. (B) Densitometric analysis shows that renal expression of V2R protein in the empagliflozin-treated OLETF group was significantly increased compared with untreated OLETF or lixisenatide-treated OLETF rats. ∗ P

    Article Snippet: For immunofluorescence, the deparaffinized kidney sections were incubated with primary antibody against AQP2 (Alomone Labs), followed by nuclear staining with 4′,6-diamidino-2-phenylindole.

    Techniques: Expressing