antibiotic antimycotic  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Thermo Fisher antibiotic antimycotic
    Antibiotic Antimycotic, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibiotic antimycotic/product/Thermo Fisher
    Average 99 stars, based on 40 article reviews
    Price from $9.99 to $1999.99
    antibiotic antimycotic - by Bioz Stars, 2022-10
    99/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher fresh neurobasal medium
    Schematic representation of experimental design: treating SCSC with MSC CM versus control medium. A) Mice were dissected to remove the spinal cord and the lumbar region sliced at 350 μm intervals. Slices were washed and placed individually in well inserts. B) SCSC were equilibrated in high serum and <t>Neurobasal</t> media prior to treating with either MSC CM or control medium. C) After 3 days of treatment with MSC CM or control medium, SCSC were fixed and immunohistochemistry performed before mounting on slides or D) incubated with Oregon Green 488 BAPTA-1AM prior to calcium imaging analysis. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
    Fresh Neurobasal Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fresh neurobasal medium/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fresh neurobasal medium - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    94
    Thermo Fisher mouse tlr4 antibody
    Diagram depicting the possible activation of TM detoxification of xenobiotics. The schematic illustrates the multiple receptors involved in the uptake and removal of potentially toxic substances in aqueous. First, two separate signaling pathways activate <t>TLR4.</t> The classic CD14-MD2-TLR4 complex is activated by the agonists lipopolysaccharide (LPS) and β-amyloid. The alternate complex of CD44-MD2-TLR4 is modulated by low-molecular-weight hyaluronic acid (LMW-HA). ABCB1 is a first-line defense and is rapidly upregulated by extracellular, e.g., β-amyloid or dephosphorylated lipopolysaccharide (LPS-p), and intracellular stress arms [ 57 ]. Xenobiotics enter through several different organic anion transporter proteins including OATP, OAT, and OCT [ 56 ]. The xenobiotic is then shuttled to the endoplasmic reticulum where cytochrome P450 3A5 or other cytochrome P450 enzymes process the xenobiotic. The second step of the detoxification pathway is ABCB1 efflux on the inactivated xenobiotic. Prolonged or overwhelming stressors, e.g., lactate accumulation, aging, or cytokine stimulation, may overwhelm the ABCB1 defense mechanism leading to faulted removal of xenobiotic, activation of NF-κB [ 52 ], and cell death. Modulators of ABCB1 are digoxin, verapamil, and the TLR4 antagonist, naloxone, which inhibits both signaling pathways.
    Mouse Tlr4 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse tlr4 antibody/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse tlr4 antibody - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    99
    Thermo Fisher hsa mir 122 anti mir mirna inhibitor
    Schematics of exosome mediated HCV transmission and possible therpaeutic strategies. HCV infected hepatocytes form multivesicular bodies (MVB) by taking up bits of the cytoplasm and its contents (proteins [HSP90], <t>miRNA</t> <t>[miR-122]</t> and RNAs [HCV RNA]) into membrane-bound vesicles. These MVB in HCV infected hepatocytes then fuse with the plasma membrane and release their contents, including numerous small vesicles (exosomes), outside the cell. Exosomes released from HCV infected hepatocytes contain replication competent HCV RNA in complex with miR-122, Ago2, and HSP90. Further, exosomes released from HCV infected hepatocytes are capable of CD81-independent HCV transmission to a naïve hepatocyte. The use of HSP90, miR-122, proton pump and Vacuolar-type H+-ATPase inhibitors can significantly limit the capacity of HCV transmission by exosomes.
    Hsa Mir 122 Anti Mir Mirna Inhibitor, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hsa mir 122 anti mir mirna inhibitor/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hsa mir 122 anti mir mirna inhibitor - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher antibiotic 100x reagent
    Effect of supernatants of cell lines U373, MDA-MB-231, and MCF-7 stimulated with TNF- α on neovascularization in culture of human endothelial cells HMEC1: (a) addition of 10 μ L of culture medium MCDB131 supplemented with 10% SFB and 1% Anti-Anti <t>100x</t> reagent; (b) addition of 5 μ L of VEGF reagent; (c) addition of 10 μ L of SNs of the U373 cell line stimulated with TNF- α ; (d) addition of 10 μ L of SNs from the U373 cell line infected with RID Adenovirus but not stimulated with TNF- α ; (e) addition of 10 μ L of SNs from the U373 cell line stimulated with TNF- α and infected with the RID Adenovirus; (f) addition of 10 μ L of SNs of the cell line MDA-MB-231 stimulated with TNF- α ; (g) addition of 10 μ L of SNs of the MDA-MB-231 cell line infected with RID Adenovirus, but not stimulated with TNF- α ; (h) addition of 10 μ L of SNs of the MDA-MB-231 cell line infected with the RID adenovirus and stimulated with TNF- α ; (i) addition of 10 μ L of SNs of the MCF-7 cell line stimulated with TNF- α ; (j) addition of 10 μ L of SNs of the MCF-7 cell line infected with RID Adenovirus, but not stimulated with TNF- α ; (k) effect of adding 10 μ L of SNs of the MCF-7 cell line infected with the RID Adenovirus and stimulated with TNF- α .
    Antibiotic 100x Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibiotic 100x reagent/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibiotic 100x reagent - by Bioz Stars, 2022-10
    99/100 stars
      Buy from Supplier

    Image Search Results


    Schematic representation of experimental design: treating SCSC with MSC CM versus control medium. A) Mice were dissected to remove the spinal cord and the lumbar region sliced at 350 μm intervals. Slices were washed and placed individually in well inserts. B) SCSC were equilibrated in high serum and Neurobasal media prior to treating with either MSC CM or control medium. C) After 3 days of treatment with MSC CM or control medium, SCSC were fixed and immunohistochemistry performed before mounting on slides or D) incubated with Oregon Green 488 BAPTA-1AM prior to calcium imaging analysis. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Biochemistry and Biophysics Reports

    Article Title: Mesenchymal stem cell conditioned medium increases glial reactivity and decreases neuronal survival in spinal cord slice cultures

    doi: 10.1016/j.bbrep.2021.100976

    Figure Lengend Snippet: Schematic representation of experimental design: treating SCSC with MSC CM versus control medium. A) Mice were dissected to remove the spinal cord and the lumbar region sliced at 350 μm intervals. Slices were washed and placed individually in well inserts. B) SCSC were equilibrated in high serum and Neurobasal media prior to treating with either MSC CM or control medium. C) After 3 days of treatment with MSC CM or control medium, SCSC were fixed and immunohistochemistry performed before mounting on slides or D) incubated with Oregon Green 488 BAPTA-1AM prior to calcium imaging analysis. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: After 4 days of equilibration, fresh Neurobasal medium (supplemented with B27 2%, l -glutamine 2%, and antibiotics/antimycotics 1%; all Gibco®) was added to wells and slices incubated a further 3 days.

    Techniques: Mouse Assay, Immunohistochemistry, Incubation, Imaging

    Diagram depicting the possible activation of TM detoxification of xenobiotics. The schematic illustrates the multiple receptors involved in the uptake and removal of potentially toxic substances in aqueous. First, two separate signaling pathways activate TLR4. The classic CD14-MD2-TLR4 complex is activated by the agonists lipopolysaccharide (LPS) and β-amyloid. The alternate complex of CD44-MD2-TLR4 is modulated by low-molecular-weight hyaluronic acid (LMW-HA). ABCB1 is a first-line defense and is rapidly upregulated by extracellular, e.g., β-amyloid or dephosphorylated lipopolysaccharide (LPS-p), and intracellular stress arms [ 57 ]. Xenobiotics enter through several different organic anion transporter proteins including OATP, OAT, and OCT [ 56 ]. The xenobiotic is then shuttled to the endoplasmic reticulum where cytochrome P450 3A5 or other cytochrome P450 enzymes process the xenobiotic. The second step of the detoxification pathway is ABCB1 efflux on the inactivated xenobiotic. Prolonged or overwhelming stressors, e.g., lactate accumulation, aging, or cytokine stimulation, may overwhelm the ABCB1 defense mechanism leading to faulted removal of xenobiotic, activation of NF-κB [ 52 ], and cell death. Modulators of ABCB1 are digoxin, verapamil, and the TLR4 antagonist, naloxone, which inhibits both signaling pathways.

    Journal: Molecular Vision

    Article Title: ABCB1 transporter and Toll-like receptor 4 in trabecular meshwork cells

    doi:

    Figure Lengend Snippet: Diagram depicting the possible activation of TM detoxification of xenobiotics. The schematic illustrates the multiple receptors involved in the uptake and removal of potentially toxic substances in aqueous. First, two separate signaling pathways activate TLR4. The classic CD14-MD2-TLR4 complex is activated by the agonists lipopolysaccharide (LPS) and β-amyloid. The alternate complex of CD44-MD2-TLR4 is modulated by low-molecular-weight hyaluronic acid (LMW-HA). ABCB1 is a first-line defense and is rapidly upregulated by extracellular, e.g., β-amyloid or dephosphorylated lipopolysaccharide (LPS-p), and intracellular stress arms [ 57 ]. Xenobiotics enter through several different organic anion transporter proteins including OATP, OAT, and OCT [ 56 ]. The xenobiotic is then shuttled to the endoplasmic reticulum where cytochrome P450 3A5 or other cytochrome P450 enzymes process the xenobiotic. The second step of the detoxification pathway is ABCB1 efflux on the inactivated xenobiotic. Prolonged or overwhelming stressors, e.g., lactate accumulation, aging, or cytokine stimulation, may overwhelm the ABCB1 defense mechanism leading to faulted removal of xenobiotic, activation of NF-κB [ 52 ], and cell death. Modulators of ABCB1 are digoxin, verapamil, and the TLR4 antagonist, naloxone, which inhibits both signaling pathways.

    Article Snippet: Blots were blocked with 5% fat-free dry milk in Tris-buffered saline-Tween (TBST) buffer for 1 h and then incubated overnight with mouse ABCB1 antibody (Santa Cruz Biotechnology, Dallas, TX; 1:1,000 dilution), mouse ABCC1 antibody (Novus Biologics, Littleton, CO; 1:500 dilution), mouse TLR4 antibody (R and D, Minneapolis MN; 1:1,000), or CYP3A5 (Thermo Scientific, Rockford, IL; 1:500).

    Techniques: Activation Assay, Molecular Weight

    Calcein AM assay of TM cells following treatment with HA and naloxone. A : Trabecular meshwork (TM) cells were treated with the TLR4 agonist low-molecular-weight hyaluronic acid (LMW-HA; 100 ng) and high-molecular-weight HA (HMW-HA; 100 ng) for 1 and 2 h. B : TM cells were treated with a TLR4 inhibitor naloxone (100 µM) alone or in combination. Error bars are presented as ± standard error of the mean (SEM), *p

    Journal: Molecular Vision

    Article Title: ABCB1 transporter and Toll-like receptor 4 in trabecular meshwork cells

    doi:

    Figure Lengend Snippet: Calcein AM assay of TM cells following treatment with HA and naloxone. A : Trabecular meshwork (TM) cells were treated with the TLR4 agonist low-molecular-weight hyaluronic acid (LMW-HA; 100 ng) and high-molecular-weight HA (HMW-HA; 100 ng) for 1 and 2 h. B : TM cells were treated with a TLR4 inhibitor naloxone (100 µM) alone or in combination. Error bars are presented as ± standard error of the mean (SEM), *p

    Article Snippet: Blots were blocked with 5% fat-free dry milk in Tris-buffered saline-Tween (TBST) buffer for 1 h and then incubated overnight with mouse ABCB1 antibody (Santa Cruz Biotechnology, Dallas, TX; 1:1,000 dilution), mouse ABCC1 antibody (Novus Biologics, Littleton, CO; 1:500 dilution), mouse TLR4 antibody (R and D, Minneapolis MN; 1:1,000), or CYP3A5 (Thermo Scientific, Rockford, IL; 1:500).

    Techniques: Calcein AM Assay, Molecular Weight

    Calcein AM assay of TM cells following treatment with lipopolysaccharide (LPS), dephosphorylated lipopolysaccharide (LPS-p), and naloxone. Trabecular meshwork (TM) cells were treated with TLR4 agonist lipopolysaccharide (LPS; 100 ng), dephosphorylated lipopolysaccharide (LPS-p; 100 ng), and the TLR4 inhibitor naloxone (100 µM) alone or in combination. Error bars are presented as ± standard error of the mean (SEM), ***p

    Journal: Molecular Vision

    Article Title: ABCB1 transporter and Toll-like receptor 4 in trabecular meshwork cells

    doi:

    Figure Lengend Snippet: Calcein AM assay of TM cells following treatment with lipopolysaccharide (LPS), dephosphorylated lipopolysaccharide (LPS-p), and naloxone. Trabecular meshwork (TM) cells were treated with TLR4 agonist lipopolysaccharide (LPS; 100 ng), dephosphorylated lipopolysaccharide (LPS-p; 100 ng), and the TLR4 inhibitor naloxone (100 µM) alone or in combination. Error bars are presented as ± standard error of the mean (SEM), ***p

    Article Snippet: Blots were blocked with 5% fat-free dry milk in Tris-buffered saline-Tween (TBST) buffer for 1 h and then incubated overnight with mouse ABCB1 antibody (Santa Cruz Biotechnology, Dallas, TX; 1:1,000 dilution), mouse ABCC1 antibody (Novus Biologics, Littleton, CO; 1:500 dilution), mouse TLR4 antibody (R and D, Minneapolis MN; 1:1,000), or CYP3A5 (Thermo Scientific, Rockford, IL; 1:500).

    Techniques: Calcein AM Assay

    Western blots of ABCB1, ABCC1, TLR4, and CYP3A5. Cell lysates (10 µg protein load) of RAW 264.7 mouse monocyte cells (RAW), rat pheochromocytoma cells (PC12), and trabecular meshwork (TM) cells were resolved with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). β-actin (10 µg protein load) was used as a loading control for each cell lysate. Data represent a representative western blot of replicate samples.

    Journal: Molecular Vision

    Article Title: ABCB1 transporter and Toll-like receptor 4 in trabecular meshwork cells

    doi:

    Figure Lengend Snippet: Western blots of ABCB1, ABCC1, TLR4, and CYP3A5. Cell lysates (10 µg protein load) of RAW 264.7 mouse monocyte cells (RAW), rat pheochromocytoma cells (PC12), and trabecular meshwork (TM) cells were resolved with sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). β-actin (10 µg protein load) was used as a loading control for each cell lysate. Data represent a representative western blot of replicate samples.

    Article Snippet: Blots were blocked with 5% fat-free dry milk in Tris-buffered saline-Tween (TBST) buffer for 1 h and then incubated overnight with mouse ABCB1 antibody (Santa Cruz Biotechnology, Dallas, TX; 1:1,000 dilution), mouse ABCC1 antibody (Novus Biologics, Littleton, CO; 1:500 dilution), mouse TLR4 antibody (R and D, Minneapolis MN; 1:1,000), or CYP3A5 (Thermo Scientific, Rockford, IL; 1:500).

    Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, SDS Page

    Schematics of exosome mediated HCV transmission and possible therpaeutic strategies. HCV infected hepatocytes form multivesicular bodies (MVB) by taking up bits of the cytoplasm and its contents (proteins [HSP90], miRNA [miR-122] and RNAs [HCV RNA]) into membrane-bound vesicles. These MVB in HCV infected hepatocytes then fuse with the plasma membrane and release their contents, including numerous small vesicles (exosomes), outside the cell. Exosomes released from HCV infected hepatocytes contain replication competent HCV RNA in complex with miR-122, Ago2, and HSP90. Further, exosomes released from HCV infected hepatocytes are capable of CD81-independent HCV transmission to a naïve hepatocyte. The use of HSP90, miR-122, proton pump and Vacuolar-type H+-ATPase inhibitors can significantly limit the capacity of HCV transmission by exosomes.

    Journal: PLoS Pathogens

    Article Title: Exosomes from Hepatitis C Infected Patients Transmit HCV Infection and Contain Replication Competent Viral RNA in Complex with Ago2-miR122-HSP90

    doi: 10.1371/journal.ppat.1004424

    Figure Lengend Snippet: Schematics of exosome mediated HCV transmission and possible therpaeutic strategies. HCV infected hepatocytes form multivesicular bodies (MVB) by taking up bits of the cytoplasm and its contents (proteins [HSP90], miRNA [miR-122] and RNAs [HCV RNA]) into membrane-bound vesicles. These MVB in HCV infected hepatocytes then fuse with the plasma membrane and release their contents, including numerous small vesicles (exosomes), outside the cell. Exosomes released from HCV infected hepatocytes contain replication competent HCV RNA in complex with miR-122, Ago2, and HSP90. Further, exosomes released from HCV infected hepatocytes are capable of CD81-independent HCV transmission to a naïve hepatocyte. The use of HSP90, miR-122, proton pump and Vacuolar-type H+-ATPase inhibitors can significantly limit the capacity of HCV transmission by exosomes.

    Article Snippet: siRNA and miRNA inhibitor constructs and cell transfections The following siRNA and miRNA inhibitors were used: human HSP90 siRNA (Santa Cruz cat. #sc-35608); control siRNA (Santa Cruz cat. #sc-44236), hsa-miR-122 anti-miR miRNA Inhibitor (Ambion, Austin, Tx cat. #AM11012) and anti-miR Negative Control (Ambion, Austin, Tx cat. #AM17010). miRNA or control inhibitors were complexed with the liver specific in vivo Altogen delivery reagent (Altogen Biosystems cat. #5060) which was loaded into control exosomes or HCV exosomes then co-cultured with target cells as indicated.

    Techniques: Transmission Assay, Infection

    Effect of supernatants of cell lines U373, MDA-MB-231, and MCF-7 stimulated with TNF- α on neovascularization in culture of human endothelial cells HMEC1: (a) addition of 10 μ L of culture medium MCDB131 supplemented with 10% SFB and 1% Anti-Anti 100x reagent; (b) addition of 5 μ L of VEGF reagent; (c) addition of 10 μ L of SNs of the U373 cell line stimulated with TNF- α ; (d) addition of 10 μ L of SNs from the U373 cell line infected with RID Adenovirus but not stimulated with TNF- α ; (e) addition of 10 μ L of SNs from the U373 cell line stimulated with TNF- α and infected with the RID Adenovirus; (f) addition of 10 μ L of SNs of the cell line MDA-MB-231 stimulated with TNF- α ; (g) addition of 10 μ L of SNs of the MDA-MB-231 cell line infected with RID Adenovirus, but not stimulated with TNF- α ; (h) addition of 10 μ L of SNs of the MDA-MB-231 cell line infected with the RID adenovirus and stimulated with TNF- α ; (i) addition of 10 μ L of SNs of the MCF-7 cell line stimulated with TNF- α ; (j) addition of 10 μ L of SNs of the MCF-7 cell line infected with RID Adenovirus, but not stimulated with TNF- α ; (k) effect of adding 10 μ L of SNs of the MCF-7 cell line infected with the RID Adenovirus and stimulated with TNF- α .

    Journal: Mediators of Inflammation

    Article Title: RID: Evaluation of the Possible Inhibiting Effect of the Proinflammatory Signaling Induced by TNF-α through NF-κβ and AP-1 in Two Cell Lines of Breast Cancer

    doi: 10.1155/2020/2707635

    Figure Lengend Snippet: Effect of supernatants of cell lines U373, MDA-MB-231, and MCF-7 stimulated with TNF- α on neovascularization in culture of human endothelial cells HMEC1: (a) addition of 10 μ L of culture medium MCDB131 supplemented with 10% SFB and 1% Anti-Anti 100x reagent; (b) addition of 5 μ L of VEGF reagent; (c) addition of 10 μ L of SNs of the U373 cell line stimulated with TNF- α ; (d) addition of 10 μ L of SNs from the U373 cell line infected with RID Adenovirus but not stimulated with TNF- α ; (e) addition of 10 μ L of SNs from the U373 cell line stimulated with TNF- α and infected with the RID Adenovirus; (f) addition of 10 μ L of SNs of the cell line MDA-MB-231 stimulated with TNF- α ; (g) addition of 10 μ L of SNs of the MDA-MB-231 cell line infected with RID Adenovirus, but not stimulated with TNF- α ; (h) addition of 10 μ L of SNs of the MDA-MB-231 cell line infected with the RID adenovirus and stimulated with TNF- α ; (i) addition of 10 μ L of SNs of the MCF-7 cell line stimulated with TNF- α ; (j) addition of 10 μ L of SNs of the MCF-7 cell line infected with RID Adenovirus, but not stimulated with TNF- α ; (k) effect of adding 10 μ L of SNs of the MCF-7 cell line infected with the RID Adenovirus and stimulated with TNF- α .

    Article Snippet: Human endothelial-derived cell line, HMEC1 (ATCC® CRL-3243 ™), from the American Type Cell Culture Collection (ATCC), Rockville, MD., were grown in 10 mL of MCDB 131 medium (Gibco® Life Technologies™) supplemented with GlutaMAX™ plus 10% heat-inactivated fetal bovine serum (SFB) and 1% antibiotic 100x reagent (antibiotic-antimycotic, Gibco® Life Technologies™).

    Techniques: Multiple Displacement Amplification, Infection