Journal: BioMed Research International

Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

doi: 10.1155/2020/9861459

Figure Lengend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

Article Snippet: The primary antibodies were rabbit anti-P2X1-6 (RRID/Cat: AB_2341048, #APR-022; AB_2341051, #APR-025; AB_2341052, #APR-026; AB_2341050, #APR-024; AB_2756757, #APR-027; and AB_2756758, #APR-028, respectively, diluted 1 : 200 with 0.1 M PBS, Alomone Labs, Israel).

Techniques: Expressing, Fluorescence

Journal: eLife

Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

doi: 10.7554/eLife.76387

Figure Lengend Snippet: ( A–D ) P2X7 expression analysed by Western blotting of macrophages isolated from control, EROS knockout (KO) ( A ) and gp91 phox KO mice ( B ), induced pluripotent stem cells (iPS)-derived macrophages control or EROS-deficient ( C ) and of control PLB985 cells and an EROS-deficient clone ( D ). ( E, F ) P2X7 expression in RAW264.7 cells overexpressing a FLAG-tagged EROS vector ( E ) and in HEK293 cells transiently expressing the specified constructs ( F ). ( G, H ) Interaction between EROS and P2X7 probed by immunoprecipitation (IP) of EROS from RAW264.7 EROS-FLAG macrophages followed by immunoblot (IB) for P2X7 ( G ) and by Nanoluc Binary Technology (NanoBIT) assay in live HEK293 cells expressing the LgBIT-fused EROS vector with a SmBIT-fused P2X7 vector ( H ). ( I ) P2X1 expression in macrophages isolated from EROS KO mice compared to control. n = 5 biological replicates. ( J ) P2X1 abundance upon co-transfection with EROS construct in HEK293 cells. Data are representative of hree independent experiments; error bars indicate SEM of triplicates. See also and . Figure 5—source data 1. Raw unedited blots for . Figure 5—source data 2. Raw unedited blots for . Figure 5—source data 3. Raw unedited blots for . Figure 5—source data 4. Uncropped gels used for .

Article Snippet: Antibody , Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) , Alomone , APR001 APR002 APR004 , (1:250) (1:500) (1:500).

Techniques: Expressing, Western Blot, Isolation, Knock-Out, Derivative Assay, Plasmid Preparation, Construct, Immunoprecipitation, Cotransfection

Journal: eLife

Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

doi: 10.7554/eLife.76387

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) , Alomone , APR001 APR002 APR004 , (1:250) (1:500) (1:500).

Techniques: Knock-Out, Generated, Over Expression

Journal: eLife

Article Title: EROS is a selective chaperone regulating the phagocyte NADPH oxidase and purinergic signalling

doi: 10.7554/eLife.76387

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-P2X1 (rabbit polyclonal) Anti-P2X4 (rabbit polyclonal) Anti-P2X7 (rabbit polyclonal) , Alomone , APR001 APR002 APR004 , (1:250) (1:500) (1:500).

Techniques: Knock-Out, Generated, Over Expression

Journal: Frontiers in Physiology

Article Title: Myosin 5a in the Urinary Bladder: Localization, Splice Variant Expression, and Functional Role in Neurotransmission

doi: 10.3389/fphys.2022.890102

Figure Lengend Snippet: Purinergic responses of WT and DBA bladders. (A) Comparison of the contractile response of WT (black bar) and DBA (gray bar) bladder smooth muscle to the purinergic receptor agonist, αβmeATP (10 µM). Contractions of WT and DBA bladders were not significantly different under this condition ( N = 11 WT, N = 13 DBA; p = 0.21). (B) Western blot comparing expression of the P2X 1 purinergic receptor in WT and DBA extracts from bladder tissue devoid of mucosa. β-actin, shown in bottom panel, served as loading control. Molecular weights of mass standards (in kDa) are indicated at tick marks. (C) Quantitative data for WT (black circles) and DBA (gray squares) are graphed at right. The intensity of P2X 1 receptor immunoreactivity, normalized by intensity of β-actin, was not different between groups ( N = 3, p = 0.2).

Article Snippet: The P2X 1 purinergic receptor (P2X 1 R) antibody (APR-001, RRID:AB_2040052) was from Alomone Labs (Jerusalem, Israel).

Techniques: Western Blot, Expressing

Journal: Scientific Reports

Article Title: Dysfunction of low-density neutrophils in peripheral circulation in patients with sepsis

doi: 10.1038/s41598-021-04682-x

Figure Lengend Snippet: Reduced chemotactic function of LDNs. ( A ) Neutrophil chemotaxis toward fMLP was assayed. CD (chemotactic distance, μm), CCR (chemo cell ratio, cell number of zone I + II + III/Total cells in the side well, %), CI (chemo index, cell number of zone II + III/ Total number of chemotactic cells, %). ( B – D ) The evaluation indicators CD, CCR, and CI were significantly reduced in neutrophil chemotactic function evaluations of LDN. ( E ) The expression of P2X1 on HDNs and LDNs was analyzed using flow cytometry.

Article Snippet: Antibodies used in the experiment include CD66b (G10F5, BD Pharmingen, USA), CD10 (HI10α, Biolegend, USA) CXCR4 (12G5, Biolegend, USA) and P2X1 (APR-022-F, Alomone labs, Israel).

Techniques: Chemotaxis Assay, Expressing, Flow Cytometry