anti vegf antibody  (Abcam)

 
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    Anti VEGFA antibody
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    ab46154
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    Structured Review

    Abcam anti vegf antibody
    Silencing effect of TRPC4 on expression of <t>VEGF</t> and NF-κB <t>p65.</t> The expression levels of VEGF and NF-κB p65 were determined to examine the underlying molecular mechanism. TRPC4 – transient receptor potential channel 4; siRNA – small interfering RNA; NC – negative control; VEGF – vascular endothelial growth factor.

    https://www.bioz.com/result/anti vegf antibody/product/Abcam
    Average 99 stars, based on 1 article reviews
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    anti vegf antibody - by Bioz Stars, 2021-03
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    Images

    1) Product Images from "Silencing of Transient Receptor Potential Channel 4 Alleviates oxLDL-induced Angiogenesis in Human Coronary Artery Endothelial Cells by Inhibition of VEGF and NF-κB"

    Article Title: Silencing of Transient Receptor Potential Channel 4 Alleviates oxLDL-induced Angiogenesis in Human Coronary Artery Endothelial Cells by Inhibition of VEGF and NF-κB

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    doi: 10.12659/MSM.897634

    Silencing effect of TRPC4 on expression of VEGF and NF-κB p65. The expression levels of VEGF and NF-κB p65 were determined to examine the underlying molecular mechanism. TRPC4 – transient receptor potential channel 4; siRNA – small interfering RNA; NC – negative control; VEGF – vascular endothelial growth factor.
    Figure Legend Snippet: Silencing effect of TRPC4 on expression of VEGF and NF-κB p65. The expression levels of VEGF and NF-κB p65 were determined to examine the underlying molecular mechanism. TRPC4 – transient receptor potential channel 4; siRNA – small interfering RNA; NC – negative control; VEGF – vascular endothelial growth factor.

    Techniques Used: Expressing, Small Interfering RNA, Negative Control

    2) Product Images from "Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study"

    Article Title: Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-016-0305-4

    Effects of UC-MSC-conditioned media on proliferation, directed migration, and motility of EA.hy926 cells. a Effects of UC-MSC-conditioned media on proliferation of EA.hy926 cells was determined by MTT assay. Cells were treated with UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody for 1, 2, or 3 days. Control cells were treated with growth media for 3 days. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Effects of UC-MSC-conditioned media on proliferation, directed migration, and motility of EA.hy926 cells. a Effects of UC-MSC-conditioned media on proliferation of EA.hy926 cells was determined by MTT assay. Cells were treated with UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody for 1, 2, or 3 days. Control cells were treated with growth media for 3 days. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Migration, MTT Assay

    Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10 3 cells per well) were cultured in 48-well plates coated with Matrigel. ( Right ) Representative images of morphological changes of networks. Scale bar 100 μm. ( Left ) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs ( red ) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells ( green ) were only associated with it. Scale bar 100 μm. c Cluster formation. ( Left ) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. ( Right ) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10 3 cells per well) were cultured in 48-well plates coated with Matrigel. ( Right ) Representative images of morphological changes of networks. Scale bar 100 μm. ( Left ) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs ( red ) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells ( green ) were only associated with it. Scale bar 100 μm. c Cluster formation. ( Left ) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. ( Right ) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Tube Formation Assay, Cell Culture, Labeling

    Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Cell Culture, Staining, Marker, Concentration Assay

    Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31 + cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs ( red ). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 ( green ). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)
    Figure Legend Snippet: Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31 + cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs ( red ). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 ( green ). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)

    Techniques Used: Staining, Labeling, Immunostaining

    3) Product Images from "Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study"

    Article Title: Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-016-0305-4

    Effects of UC-MSC-conditioned media on proliferation, directed migration, and motility of EA.hy926 cells. a Effects of UC-MSC-conditioned media on proliferation of EA.hy926 cells was determined by MTT assay. Cells were treated with UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody for 1, 2, or 3 days. Control cells were treated with growth media for 3 days. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Effects of UC-MSC-conditioned media on proliferation, directed migration, and motility of EA.hy926 cells. a Effects of UC-MSC-conditioned media on proliferation of EA.hy926 cells was determined by MTT assay. Cells were treated with UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody for 1, 2, or 3 days. Control cells were treated with growth media for 3 days. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Migration, MTT Assay

    Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10 3 cells per well) were cultured in 48-well plates coated with Matrigel. ( Right ) Representative images of morphological changes of networks. Scale bar 100 μm. ( Left ) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs ( red ) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells ( green ) were only associated with it. Scale bar 100 μm. c Cluster formation. ( Left ) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. ( Right ) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10 3 cells per well) were cultured in 48-well plates coated with Matrigel. ( Right ) Representative images of morphological changes of networks. Scale bar 100 μm. ( Left ) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs ( red ) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells ( green ) were only associated with it. Scale bar 100 μm. c Cluster formation. ( Left ) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. ( Right ) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Tube Formation Assay, Cell Culture, Labeling

    Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Cell Culture, Staining, Marker, Concentration Assay

    Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31 + cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs ( red ). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 ( green ). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)
    Figure Legend Snippet: Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31 + cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs ( red ). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 ( green ). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)

    Techniques Used: Staining, Labeling, Immunostaining

    4) Product Images from "Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study"

    Article Title: Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study

    Journal: Stem Cell Research & Therapy

    doi: 10.1186/s13287-016-0305-4

    Effects of UC-MSC-conditioned media on proliferation, directed migration, and motility of EA.hy926 cells. a Effects of UC-MSC-conditioned media on proliferation of EA.hy926 cells was determined by MTT assay. Cells were treated with UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody for 1, 2, or 3 days. Control cells were treated with growth media for 3 days. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Effects of UC-MSC-conditioned media on proliferation, directed migration, and motility of EA.hy926 cells. a Effects of UC-MSC-conditioned media on proliferation of EA.hy926 cells was determined by MTT assay. Cells were treated with UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody for 1, 2, or 3 days. Control cells were treated with growth media for 3 days. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Migration, MTT Assay

    Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10 3 cells per well) were cultured in 48-well plates coated with Matrigel. ( Right ) Representative images of morphological changes of networks. Scale bar 100 μm. ( Left ) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs ( red ) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells ( green ) were only associated with it. Scale bar 100 μm. c Cluster formation. ( Left ) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. ( Right ) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Matrigel tube formation assay. a UC-MSCs, or EA.hy926 cells, or 1:1 mixed UC-MSC-EA.hy926 cells in the presence of growth media, or EA.hy926 cells in the presence of UC-MSC-conditioned media or UC-MSC-conditioned media supplemented with anti-VEGF antibody (total 35 × 10 3 cells per well) were cultured in 48-well plates coated with Matrigel. ( Right ) Representative images of morphological changes of networks. Scale bar 100 μm. ( Left ) Quantification of lengths of the tubes and numbers of branch points. Values are expressed as average ± SD. b PKH26-labeled UC-MSCs ( red ) became the basis of a mixed culture network, while PKH67-labeled EA.hy926 cells ( green ) were only associated with it. Scale bar 100 μm. c Cluster formation. ( Left ) Representative images of tight clusters formed in Matrigel by UC-MSCs, or EA.hy926 cells, or mixed UC-MSC-EA.hy926 cells 24 hours after seeding. Macrophotograph of the 48-well plate. ( Right ) Quantification of numbers of clusters. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Tube Formation Assay, Cell Culture, Labeling

    Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. * p
    Figure Legend Snippet: Cell culture characterization. a Human UC-MSC immunophenotype, positive for CD73, CD90, and CD105 and negative for CD45, CD34, CD11b, CD19, and HLA-DR. b Multilineage differentiation of UC-MSCs. Differentiation into adipocytes was revealed by Sudan III staining for intracellular accumulated lipids. Differentiation into osteocytes was revealed by Alizarin Red S staining for calcium mineralization. Chondrogenic differentiation was revealed by Alcian blue staining for mucopolysaccharides. Scale bar 100 μm. c Positive staining of EA.hy926 cells for CD31 as endothelial marker. Scale bar 100 μm. d Concentration of soluble forms of VEGF-A (VEGF-A-121 and VEGF-A-165) in the EA.hy926-conditioned media and the UC-MSC-conditioned media. Values are expressed as average ± SD of three replicates. * p

    Techniques Used: Cell Culture, Staining, Marker, Concentration Assay

    Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31 + cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs ( red ). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 ( green ). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)
    Figure Legend Snippet: Endothelial differentiation of UC-MSCs. a Endothelial differentiation of UC-MSCs in monolayer. Three endothelial induction media were used for UC-MSC culture: EA.hy926-conditioned media mixed 1:1 with growth media; EA.hy926-conditioned media mixed 1:1 with growth media supplemented with VEGF-A-165 (50 ng/ml); and growth media supplemented with VEGF-A-165. Only EA.hy926-conditioned media supplemented with VEGF-A-165 led to the appearance of CD31 + cells in the culture. Cell nuclei were stained with DAPI. Scale bar 100 μm. b Endothelial differentiation of UC-MSCs in Matrigel. Cryosections of secondary sprouts promoted by PKH26-labeled UC-MSCs ( red ). Immunostaining of sections showed that, upon coculturing with EA.hy926 in Matrigel, the UC-MSCs started to express CD31 ( green ). Cell nuclei were stained with DAPI. Scale bar 100 μm. VEGF vascular endothelial growth factor (Color figure online)

    Techniques Used: Staining, Labeling, Immunostaining

    Related Articles

    Immunohistochemistry:

    Article Title: Neuropilin-2 promotes branching morphogenesis in the mouse mammary gland
    Article Snippet: .. Antibodies (Abs, 1 μg/ml) specific for either E-cadherin (4A2C7, Invitrogen), Ki67 (NCL-Ki67p, Leica Microsystems), phospho-Tyr 397-FAK (p-FAK, Biosource) or VEGF (Ab46154, Abcam) were used for immunohistochemistry. .. Briefly, 5-week-old mammary gland sections on Superfrost Plus microscope slides (48312-501, VWR International) were steamed for 30 minutes in a citrate buffer (pH 6.0; Zymed/Invitrogen, Carlsbad, CA, USA) for antigen retrieval.

    Blocking Assay:

    Article Title: Down-regulation of HIF-1α inhibits the proliferation, migration, and invasion of gastric cancer by inhibiting PI3K/AKT pathway and VEGF expression
    Article Snippet: After that, 10% SDS/PAGE gel electrophoresis was carried out using 20 µg protein from each sample, followed by gel transfer to PVDF membranes. .. After blocking with 5% skimmed milk at room temperature for 1 h, PDVF membranes were washed and incubated with primary antibodies including rabbit anti-AKT antibody (1:2000, ab126811, Abcam), rabbit anti-phosphor-AKT antibody (p-T308, 1:2000, ab38449, Abcam), rabbit anti-VEGF antibody (1:2000, ab46154, Abcam), and anti-GAPDH antibody (1:1000, ab9485, Abcam) overnight at 4°C. .. The next day, membranes were washed with TBST and incubated with anti-rabbit IgG-HRP secondary antibody (1:1000, MBS435036, MyBioSource) at room temperature for 2.5 h. Then membranes were washed again with TBST, and signals were detected by ECL (Sigma–Aldrich, U.S.A.) method.

    Incubation:

    Article Title: Down-regulation of HIF-1α inhibits the proliferation, migration, and invasion of gastric cancer by inhibiting PI3K/AKT pathway and VEGF expression
    Article Snippet: After that, 10% SDS/PAGE gel electrophoresis was carried out using 20 µg protein from each sample, followed by gel transfer to PVDF membranes. .. After blocking with 5% skimmed milk at room temperature for 1 h, PDVF membranes were washed and incubated with primary antibodies including rabbit anti-AKT antibody (1:2000, ab126811, Abcam), rabbit anti-phosphor-AKT antibody (p-T308, 1:2000, ab38449, Abcam), rabbit anti-VEGF antibody (1:2000, ab46154, Abcam), and anti-GAPDH antibody (1:1000, ab9485, Abcam) overnight at 4°C. .. The next day, membranes were washed with TBST and incubated with anti-rabbit IgG-HRP secondary antibody (1:1000, MBS435036, MyBioSource) at room temperature for 2.5 h. Then membranes were washed again with TBST, and signals were detected by ECL (Sigma–Aldrich, U.S.A.) method.

    Article Title: Thioredoxin-interacting protein promotes high-glucose-induced macrovascular endothelial dysfunction
    Article Snippet: .. The PVDF membranes were incubated with anti-ChREBP (Cat#sc-21189; Santa Cruz Biotechnology, USA), anti-TXNIP (Cat#40–3700; Invitrogen, USA), anti-VEGF (Cat#ab46154; abcam, USA), anti-VCAM-1 (Cat#AF643; R & D Systems, Minneapolis, USA), anti-Cleaved Caspase-3 (Cat#9661S; Cell Signaling Technology, USA), or anti-β-Actin (Cat#A5441; Sigma-Aldrich) at 4°C overnight. .. Immunoreactive bands were quantified by the NIH ImageJ software.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Thioredoxin-interacting protein promotes high-glucose-induced macrovascular endothelial dysfunction
    Article Snippet: .. The PVDF membranes were incubated with anti-ChREBP (Cat#sc-21189; Santa Cruz Biotechnology, USA), anti-TXNIP (Cat#40–3700; Invitrogen, USA), anti-VEGF (Cat#ab46154; abcam, USA), anti-VCAM-1 (Cat#AF643; R & D Systems, Minneapolis, USA), anti-Cleaved Caspase-3 (Cat#9661S; Cell Signaling Technology, USA), or anti-β-Actin (Cat#A5441; Sigma-Aldrich) at 4°C overnight. .. Immunoreactive bands were quantified by the NIH ImageJ software.

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  • 99
    Abcam anti vegf a
    Conformer selectivity of the peptides for different oncogenic quadruplexes in MCF-7 cells. ( A ) pGL4.72[ hRlucCP ] vector having the inserts containing oncogenic promoter sequences ( c-MYC, BCL-2, KRAS and <t>VEGF-A</t> ) and upstream G-quadruplex forming elements ahead of the hRluc coding region. The promoter sequences are cloned into KpnI and HindIII restriction sites with or without the wild-type quadruplex scaffolds. hRluc, Renilla luciferase gene; hCL1 and hPEST , protein destabilizing sequences; oriC, origin of replication; AmpR, ampicillin resistance gene; SV40 (Simian virus 40 polyadenylation signal cassette), P1 and P2, promoter sequences; null, constructs having no quadruplex motif upstream the oncogene promoters; GQ, G-quadruplex forming motif. ( B ) Dual-luciferase assays. Evaluation of the promoter activity of different oncogenes (( B ) c-MYC , ( C ) BCL-2 , ( D ) KRAS and ( E ) VEGF-A ) of using the reporter plasmids with or without the wild-type quadruplex-forming sequences (Pu27-GQ, BCL-2-GQ, KRAS-GQ and VEGF-A-GQ) in MCF-7 cells. Relative promoter activity is determined by normalizing the Rluc/Fluc values to that of the cells transfected with P1-P2 promoter construct (GQ-null), having no quadruplex-forming motif. Error bars represent mean ± SE ( N = 5). Statistical differences in the luciferase activities compared to the respective GQ-null constructs of the target oncogenes used one-way ANOVA followed by Tukey–Kramer Test ( # P
    Anti Vegf A, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti vegf a/product/Abcam
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    vegf  (Abcam)
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    Abcam vegf
    RT-qPCR and Western blot analysis demonstrated that MBNL1-AS1 reduced the expression of KCNMA1 and cGMP-PKG signaling pathway-related genes. a RT-qPCR measured the relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, <t>VASP,</t> <t>VEGF,</t> Bax, and Bcl-2 in skeletal muscle cells after transfection. b Protein bands measured by Western blot analysis. c Western blot analysis measured the relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, p-PKGII phosphorylated-PKGII, VASP vasodilator-stimulated phosphoprotein, p-VASP phosphorylated-VASP, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, si small interfering, GAPDH glyceraldehyde-3-phosphate dehydrogenase, NC negative control; * p
    Vegf, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vegf/product/Abcam
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    vegf - by Bioz Stars, 2021-03
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    99
    Abcam rabbit anti vegf a
    Lack of p73 affects TGF- β signaling in vivo . Quantification of <t>VEGF-A</t> expression and analysis of the TGF -β /ALK1/ID1 signaling pathway in P5 retinas from WT and p73KO mice. qRT-PCR analysis demonstrated a significant decrease in VEGF-A
    Rabbit Anti Vegf A, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vegf a/product/Abcam
    Average 99 stars, based on 1 article reviews
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    Conformer selectivity of the peptides for different oncogenic quadruplexes in MCF-7 cells. ( A ) pGL4.72[ hRlucCP ] vector having the inserts containing oncogenic promoter sequences ( c-MYC, BCL-2, KRAS and VEGF-A ) and upstream G-quadruplex forming elements ahead of the hRluc coding region. The promoter sequences are cloned into KpnI and HindIII restriction sites with or without the wild-type quadruplex scaffolds. hRluc, Renilla luciferase gene; hCL1 and hPEST , protein destabilizing sequences; oriC, origin of replication; AmpR, ampicillin resistance gene; SV40 (Simian virus 40 polyadenylation signal cassette), P1 and P2, promoter sequences; null, constructs having no quadruplex motif upstream the oncogene promoters; GQ, G-quadruplex forming motif. ( B ) Dual-luciferase assays. Evaluation of the promoter activity of different oncogenes (( B ) c-MYC , ( C ) BCL-2 , ( D ) KRAS and ( E ) VEGF-A ) of using the reporter plasmids with or without the wild-type quadruplex-forming sequences (Pu27-GQ, BCL-2-GQ, KRAS-GQ and VEGF-A-GQ) in MCF-7 cells. Relative promoter activity is determined by normalizing the Rluc/Fluc values to that of the cells transfected with P1-P2 promoter construct (GQ-null), having no quadruplex-forming motif. Error bars represent mean ± SE ( N = 5). Statistical differences in the luciferase activities compared to the respective GQ-null constructs of the target oncogenes used one-way ANOVA followed by Tukey–Kramer Test ( # P

    Journal: Nucleic Acids Research

    Article Title: Site-specific amino acid substitution in dodecameric peptides determines the stability and unfolding of c-MYC quadruplex promoting apoptosis in cancer cells

    doi: 10.1093/nar/gky824

    Figure Lengend Snippet: Conformer selectivity of the peptides for different oncogenic quadruplexes in MCF-7 cells. ( A ) pGL4.72[ hRlucCP ] vector having the inserts containing oncogenic promoter sequences ( c-MYC, BCL-2, KRAS and VEGF-A ) and upstream G-quadruplex forming elements ahead of the hRluc coding region. The promoter sequences are cloned into KpnI and HindIII restriction sites with or without the wild-type quadruplex scaffolds. hRluc, Renilla luciferase gene; hCL1 and hPEST , protein destabilizing sequences; oriC, origin of replication; AmpR, ampicillin resistance gene; SV40 (Simian virus 40 polyadenylation signal cassette), P1 and P2, promoter sequences; null, constructs having no quadruplex motif upstream the oncogene promoters; GQ, G-quadruplex forming motif. ( B ) Dual-luciferase assays. Evaluation of the promoter activity of different oncogenes (( B ) c-MYC , ( C ) BCL-2 , ( D ) KRAS and ( E ) VEGF-A ) of using the reporter plasmids with or without the wild-type quadruplex-forming sequences (Pu27-GQ, BCL-2-GQ, KRAS-GQ and VEGF-A-GQ) in MCF-7 cells. Relative promoter activity is determined by normalizing the Rluc/Fluc values to that of the cells transfected with P1-P2 promoter construct (GQ-null), having no quadruplex-forming motif. Error bars represent mean ± SE ( N = 5). Statistical differences in the luciferase activities compared to the respective GQ-null constructs of the target oncogenes used one-way ANOVA followed by Tukey–Kramer Test ( # P

    Article Snippet: Santacruz Biotechnology Inc.), anti E2F1, anti VEGF-A, anti-P53, anti-APAF1, anti-Caspase 8, anti-PARP (Abcam), anti-β-actin (Mouse monoclonal, 1:10 000 dil.

    Techniques: Plasmid Preparation, Clone Assay, Luciferase, Construct, Activity Assay, Transfection

    Selective arresting of c-MYC quadruplex by KR12C induce apoptotic signalling in cancer cells. ( A ) Western blot analyses of the major apoptotic markers (BCL-2, APAF-1, caspase 8 and PARP). ( B ) Estimation of protein expression level of the apoptotic markers by semi-densitometric analyses. Estimation of BCL-2 transcripts by real time PCR analyses at 5 and 10 μM KR12C concentration. ( C ) Western blot analyses of other proteins associated with the signalling cascade (c-MYC, E2F-1, VEGF-A and P53). ( D ) Quatification of protein and mRNA expression level of c-MYC, E2F-1, VEGF-A and P53 by semi-densitometric analyses and real time PCR respectively. Arrow pointing upwards and downwards denote the upregulation of the respective protein and mRNA expression level. Error bars represent mean ± SE ( N = 3). Statistical differences are determined compared to the control by two-tailed Student's t test (* P

    Journal: Nucleic Acids Research

    Article Title: Site-specific amino acid substitution in dodecameric peptides determines the stability and unfolding of c-MYC quadruplex promoting apoptosis in cancer cells

    doi: 10.1093/nar/gky824

    Figure Lengend Snippet: Selective arresting of c-MYC quadruplex by KR12C induce apoptotic signalling in cancer cells. ( A ) Western blot analyses of the major apoptotic markers (BCL-2, APAF-1, caspase 8 and PARP). ( B ) Estimation of protein expression level of the apoptotic markers by semi-densitometric analyses. Estimation of BCL-2 transcripts by real time PCR analyses at 5 and 10 μM KR12C concentration. ( C ) Western blot analyses of other proteins associated with the signalling cascade (c-MYC, E2F-1, VEGF-A and P53). ( D ) Quatification of protein and mRNA expression level of c-MYC, E2F-1, VEGF-A and P53 by semi-densitometric analyses and real time PCR respectively. Arrow pointing upwards and downwards denote the upregulation of the respective protein and mRNA expression level. Error bars represent mean ± SE ( N = 3). Statistical differences are determined compared to the control by two-tailed Student's t test (* P

    Article Snippet: Santacruz Biotechnology Inc.), anti E2F1, anti VEGF-A, anti-P53, anti-APAF1, anti-Caspase 8, anti-PARP (Abcam), anti-β-actin (Mouse monoclonal, 1:10 000 dil.

    Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Two Tailed Test

    Schematic representation showing selective quadruplex interaction at c-MYC promoter by KR12C promotes apoptotic signalling in VEGF-A-BCL-2 axis in MCF-7 cells.

    Journal: Nucleic Acids Research

    Article Title: Site-specific amino acid substitution in dodecameric peptides determines the stability and unfolding of c-MYC quadruplex promoting apoptosis in cancer cells

    doi: 10.1093/nar/gky824

    Figure Lengend Snippet: Schematic representation showing selective quadruplex interaction at c-MYC promoter by KR12C promotes apoptotic signalling in VEGF-A-BCL-2 axis in MCF-7 cells.

    Article Snippet: Santacruz Biotechnology Inc.), anti E2F1, anti VEGF-A, anti-P53, anti-APAF1, anti-Caspase 8, anti-PARP (Abcam), anti-β-actin (Mouse monoclonal, 1:10 000 dil.

    Techniques:

    RT-qPCR and Western blot analysis demonstrated that MBNL1-AS1 reduced the expression of KCNMA1 and cGMP-PKG signaling pathway-related genes. a RT-qPCR measured the relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. b Protein bands measured by Western blot analysis. c Western blot analysis measured the relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, p-PKGII phosphorylated-PKGII, VASP vasodilator-stimulated phosphoprotein, p-VASP phosphorylated-VASP, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, si small interfering, GAPDH glyceraldehyde-3-phosphate dehydrogenase, NC negative control; * p

    Journal: Experimental & Molecular Medicine

    Article Title: Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1

    doi: 10.1038/s12276-018-0133-y

    Figure Lengend Snippet: RT-qPCR and Western blot analysis demonstrated that MBNL1-AS1 reduced the expression of KCNMA1 and cGMP-PKG signaling pathway-related genes. a RT-qPCR measured the relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. b Protein bands measured by Western blot analysis. c Western blot analysis measured the relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle cells after transfection. MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily M alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, p-PKGII phosphorylated-PKGII, VASP vasodilator-stimulated phosphoprotein, p-VASP phosphorylated-VASP, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, si small interfering, GAPDH glyceraldehyde-3-phosphate dehydrogenase, NC negative control; * p

    Article Snippet: The membrane was then blocked in 5% skimmed milk at room temperature for 1 h, washed twice with PBS, and then incubated overnight at 4 °C with diluted rabbit anti-mouse primary antibodies against KCNMA1 (1:1000, MAB8589; AmyJet Scientific Inc., Wuhan, China), PKGII (1:1000, ab145063; Abcam, Inc., Cambridge, MA, USA), VASP (1:1000, ab58555; Abcam, USA), VEGF (1:1000, ab32152; Abcam, USA), Bax (1:1000, ab32503; Abcam, USA), Bcl-2 (1:1000, ab59348; Abcam, USA), Cyclin D1 (1:1000, ab134175, Abcam, USA), Cyclin D3 (1:1000, ab28283, Abcam, USA), Cdc 42 (1:1000, ab187643, Abcam, USA), caspase-3 (1:1000, ab208161, Abcam, USA), and PARP (1:1000, ab32064, Abcam, USA).

    Techniques: Quantitative RT-PCR, Western Blot, Expressing, Transfection, Negative Control

    MBNL1-AS1 was highly expressed, but KCNMA1 and cGMP-PKG pathway-related factors were poorly expressed in mice with I/R injury after TKA. a Relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in the skeletal muscle tissues of mice detected by RT-qPCR. b Protein bands observed after Western blot analysis. c Relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle tissues of mice evaluated by Western blot analysis. I/R ischemia-reperfusion, Sevo sevoflurane, MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, VASP vasodilator-stimulated phosphoprotein, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase; * p

    Journal: Experimental & Molecular Medicine

    Article Title: Downregulation of the long noncoding RNA MBNL1-AS1 protects sevoflurane-pretreated mice against ischemia-reperfusion injury by targeting KCNMA1

    doi: 10.1038/s12276-018-0133-y

    Figure Lengend Snippet: MBNL1-AS1 was highly expressed, but KCNMA1 and cGMP-PKG pathway-related factors were poorly expressed in mice with I/R injury after TKA. a Relative mRNA expression of MBNL1-AS1, KCNMA1, PKGII, VASP, VEGF, Bax, and Bcl-2 in the skeletal muscle tissues of mice detected by RT-qPCR. b Protein bands observed after Western blot analysis. c Relative protein levels of KCNMA1, PKGII, VASP, p-PKGII, p-VASP, VEGF, Bax, and Bcl-2 in skeletal muscle tissues of mice evaluated by Western blot analysis. I/R ischemia-reperfusion, Sevo sevoflurane, MBNL1-AS1 muscleblind-like 1 antisense RNA 1, KCNMA1 potassium calcium-activated channel subfamily alpha 1, PKGII type II cyclic guanosine monophosphate-dependent protein kinase G, VASP vasodilator-stimulated phosphoprotein, VEGF vascular endothelial growth factor, Bcl-2 B-cell lymphoma/leukemia-2, Bax Bcl-2 associated X protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase; * p

    Article Snippet: The membrane was then blocked in 5% skimmed milk at room temperature for 1 h, washed twice with PBS, and then incubated overnight at 4 °C with diluted rabbit anti-mouse primary antibodies against KCNMA1 (1:1000, MAB8589; AmyJet Scientific Inc., Wuhan, China), PKGII (1:1000, ab145063; Abcam, Inc., Cambridge, MA, USA), VASP (1:1000, ab58555; Abcam, USA), VEGF (1:1000, ab32152; Abcam, USA), Bax (1:1000, ab32503; Abcam, USA), Bcl-2 (1:1000, ab59348; Abcam, USA), Cyclin D1 (1:1000, ab134175, Abcam, USA), Cyclin D3 (1:1000, ab28283, Abcam, USA), Cdc 42 (1:1000, ab187643, Abcam, USA), caspase-3 (1:1000, ab208161, Abcam, USA), and PARP (1:1000, ab32064, Abcam, USA).

    Techniques: Mouse Assay, Expressing, Quantitative RT-PCR, Western Blot

    VEGF-A and VEGFR-1 are important for Snail expression. ( a ) Cells were transfected with indicated vectors. Quantitative RT-PCR (mean±s.d. * P

    Journal: Experimental & Molecular Medicine

    Article Title: Rab25 augments cancer cell invasiveness through a β1 integrin/EGFR/VEGF-A/Snail signaling axis and expression of fascin

    doi: 10.1038/emm.2017.248

    Figure Lengend Snippet: VEGF-A and VEGFR-1 are important for Snail expression. ( a ) Cells were transfected with indicated vectors. Quantitative RT-PCR (mean±s.d. * P

    Article Snippet: Immunohistochemistry was performed with primary Rab25 (ab106175, 1:200, Abcam, Cambridge, MA, USA), VEGFR-1 (ab32152, 1:250, Abcam), Snail (ab180714, 1:100, Abcam) and fascin (ab126772, 1:250, Abcam) antibodies, as described previously.

    Techniques: Expressing, Transfection, Quantitative RT-PCR

    Lack of p73 affects TGF- β signaling in vivo . Quantification of VEGF-A expression and analysis of the TGF -β /ALK1/ID1 signaling pathway in P5 retinas from WT and p73KO mice. qRT-PCR analysis demonstrated a significant decrease in VEGF-A

    Journal: Cell Death and Differentiation

    Article Title: p73 is required for endothelial cell differentiation, migration and the formation of vascular networks regulating VEGF and TGFβ signaling

    doi: 10.1038/cdd.2014.214

    Figure Lengend Snippet: Lack of p73 affects TGF- β signaling in vivo . Quantification of VEGF-A expression and analysis of the TGF -β /ALK1/ID1 signaling pathway in P5 retinas from WT and p73KO mice. qRT-PCR analysis demonstrated a significant decrease in VEGF-A

    Article Snippet: Western blotting was performed as previously described with the following primary antibodies: rabbit anti-pSmad1/5 (Ser463/465) 1 : 1000 (Cell Signaling, Danvers, MA, USA), rabbit anti Smad1 1 : 1000 (Cell Signaling), mouse anti-pERK 1 : 1000 (Santa Cruz Biotechnology), rabbit anti-ERK1 : 10 000 (Santa Cruz Biotechnology), rabbit anti-VEGF-A 1 : 1000 (Abcam) mouse anti DNp73 1 : 1000 (Imgenex, San Diego, CO, USA), and rabbit anti Nanog 1 : 1000 (Chemicon, Billerica, TX, USA) followed by the appropriate HRP-conjugated secondary antibodies (Pierce, Waltham, MA, USA).

    Techniques: In Vivo, Expressing, Mouse Assay, Quantitative RT-PCR