anti vascular endothelial growth factor vegf a  (Cell Signaling Technology Inc)

 
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    Name:
    Vascular Endothelial Growth Factor VEGF
    Description:
    The human VEGF165 coding cDNA was subcloned into an expression vector and expressed in yeast The recombinant human VEGF 165 homodimer was purified and stored in PBS buffer pH 7 4 containing 0 1 BSA
    Catalog Number:
    9943
    Price:
    None
    Category:
    Cytokines
    Source:
    Human Recombinant Protein
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    Structured Review

    Cell Signaling Technology Inc anti vascular endothelial growth factor vegf a
    <t>VEGF-A</t> and -C protein expression of breast cancer MCF-7 cells in the <t>COX-2-shRNA</t> group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.
    The human VEGF165 coding cDNA was subcloned into an expression vector and expressed in yeast The recombinant human VEGF 165 homodimer was purified and stored in PBS buffer pH 7 4 containing 0 1 BSA
    https://www.bioz.com/result/anti vascular endothelial growth factor vegf a/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti vascular endothelial growth factor vegf a - by Bioz Stars, 2021-01
    99/100 stars

    Images

    1) Product Images from "Effect of cycloxygenase-2 silencing on the malignant biological behavior of MCF-7 breast cancer cells"

    Article Title: Effect of cycloxygenase-2 silencing on the malignant biological behavior of MCF-7 breast cancer cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2014.2395

    VEGF-A and -C protein expression of breast cancer MCF-7 cells in the COX-2-shRNA group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.
    Figure Legend Snippet: VEGF-A and -C protein expression of breast cancer MCF-7 cells in the COX-2-shRNA group was significantly lower than that of the blank and mock groups. COX-2, cyclooxygenase-2; VEGF, vascular endothelial growth factor; shRNA, short hairpin RNA.

    Techniques Used: Expressing, shRNA

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

    In Vivo:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

    Labeling:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

    Immunoprecipitation:

    Article Title: Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells
    Article Snippet: .. Immunoprecipitation The HUVECs were grown to confluence and stimulated with 20 ng/ml VEGF-A (Cell Signaling Technology, Inc., Beverly, MA, USA) at 37°C for 0, 20, 40 and 60 min. .. The cells were then washed with ice-cold PBS and solubilized on ice with lysis buffer containing 150 mM NaCl, 10 mM Tris-HCl, (pH 7.5) and 1% Triton X-100 supplemented with a cocktail of phosphatase and proteinase inhibitors containing 1 mM vanadate, 10 mg/ml leupeptin, 10 mg/ml aprotinin, 1 mM phenylmethylsulfonyl fluoride and 0.36 mM phenanthroline.

    Incubation:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

    Article Title: Effect of cycloxygenase-2 silencing on the malignant biological behavior of MCF-7 breast cancer cells
    Article Snippet: .. Next, the primary rabbit monoclonal anti-COX-2 (1:500), anti-vascular endothelial growth factor (VEGF)-A (1:800), anti-VEGF-C (1:800) and anti-GAPDH (1:4,000) antibodies (Cell Signaling Technology, Inc., Danvers, MA, USA) were added and the mixture was incubated overnight at 4°C on a rocking platform. .. Subsequent to being washed, the membrane was added together with the horseradish peroxidase-conjugated secondary antibody (1:4,000) and incubated for 2 h. The membrane was then developed using an enhanced chemiluminescence system (Pierce Biotechnology, Inc., Rockford, IL, USA) and exposed to X-ray film.

    Article Title: Apigenin Protects the Brain against Ischemia/Reperfusion Injury via Caveolin-1/VEGF In Vitro and In Vivo
    Article Snippet: .. After being blocked by 5% skim milk in Tris-buffered saline containing TBST, the membranes were incubated with primary antibodies against β -tubulin (Sungene Biotech, China), Caveolin-1 (Santa Cruz Biotechnology, USA), Bcl-2 (Abcam, UK), VEGF (CST, USA), eNOS (Abcam, UK), cleaved Caspase3 (CST, USA), Beclin-1 (CST), and mTOR (CST, USA) and were incubated overnight at 4°C. ..

    Expressing:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

    Western Blot:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

    SDS Page:

    Article Title: Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation
    Article Snippet: .. Western blot analysis for the expression of VEGF protein in vivo Lysates from hind limb muscle tissue homogenates harvested at day 21 post-surgery were used for Western blot analysis as described previously[ ].Protein was analyzed using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad).Membranes were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and β-actin (1:5000, Sigma) at 4°C overnight respectively.The membranes were then incubated with peroxidase labeled secondary antibody (1:1000, Santa Cruz, USA) at 37°C for 2 hours. .. Signals were detected by enhanced chemiluminescence (Amersham, USA).

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    Cell Signaling Technology Inc rabbit anti vegf
    Effect of curcumin and hypoxia on <t>IGF-1R,</t> p-Akt, p-Erk1/2, HIF-1α, <t>VEGF</t> protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P
    Rabbit Anti Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti vegf/product/Cell Signaling Technology Inc
    Average 91 stars, based on 7 article reviews
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    Cell Signaling Technology Inc rabbit anti vegf receptor 2
    Gene expression analysis of GEnCs cultured on hydrogel substrates at designated time points. (A)  PECAM1  encoding for platelet endothelial cell adhesion molecule or CD31. (B)  CDH5  encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C)  ITGB1  encoding for integrin subunit β1. (D)  ITGB3  encoding for integrin subunit β3. (E)  KDR  encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F)  TEK  encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G)  VWF  encoding for von Willebrand factor. (H)  NOS3  encoding for nitric oxide synthase 3. (I)  PLVAP  encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression  normalized to gene expression of GEnCs cultured in tissue culture polystyrene on day 0 ( n  = 4).
    Rabbit Anti Vegf Receptor 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc primary antibodies against vegf
    Effect of scutellarin and scutellarin-loaded nanoparticles on the expression of <t>VEGF,</t> <t>VEGFR2</t> and vWF. a Western blot analysis was used to determine the expression of VEGF,VEGFR2 and vWF in the rat retina. Quantitative evaluation of protein expression of VEGF/GAPDH ( b ), VEGFR2/GAPDH ( c ) and vWF/GAPDH ( d ). Data are presented as mean ± SD. *Significantly different from control group ( P
    Primary Antibodies Against Vegf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against vegf/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
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    primary antibodies against vegf - by Bioz Stars, 2021-01
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    92
    Cell Signaling Technology Inc anti vegf a
    Bevacizumab treatment concurrent with nab-paclitaxel chemotherapy is the most effective regimen for inhibiting tumor growth and metastasis. (A, B) Mice bearing 435-LuC + tumors of 450 mm 3 in volume were treated with bevacizumab (4 mg/kg) using three different combination regimens with nab-paclitaxel (10 mg/kg, three cycles of daily for five consecutive days). (A) Schematic illustration of experimental design describing different regimens assessed in this study. Group 1 received three cycles of nab-paclitaxel followed by bevacizumab for the duration of the study, group 2 received bevacizumab concurrent with nab-paclitaxel and both drugs were discontinued at the end of three cycles of nab-paclitaxel, and group 3 received bevacizumab throughout three cycles of nab-paclitaxel and for the duration of the study. (B) Black arrows indicate the start of each cycle of nab-paclitaxel treatment. Data are presented as the mean tumor volume ± SE per group at the indicated days after implantation. Frozen sections from control- (C, top) and nab-paclitaxel-treated (C, bottom) tumors were stained with <t>anti-VEGF-A</t> antibodies and counterstained with 4′,6-diamidino-2-phenylindole to visualize the nuclei. Images were acquired at a constant exposure setting and total fluorescent intensity of VEGF-A staining from control (D, top) and treated (D, bottom) tumor sections was plotted using the ImageJ software as described in the Materials and Methods. Fluorescent intensity plots of representative images in (C) are shown. All images were acquired at a magnification of x200.
    Anti Vegf A, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

    doi: 10.3892/etm.2018.5783

    Figure Lengend Snippet: Effect of curcumin and hypoxia on IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression in IGF-1R knockout HepG2 cells. (A) IGF-1R, p-Akt, p-Erk1/2, HIF-1α, VEGF protein expression were detected by western blot analysis. Graphic representation of relative density of (B) IGF-1R, (C) p-Akt, (D) p-Erk1/2, (E) HIF-1α and (F) VEGF protein levels, which were normalized to those of β-actin, Akt or Erk1/2, respectively. *P

    Article Snippet: The primary antibodies used in the experiment were as follows: Rabbit anti-IGF-1R (cat. no. 9750), rabbit anti-HIF-1α (cat. no. 3716), rabbit anti-VEGF (cat. no. 2463), rabbit anti-Akt (cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (cat. no. 5012), rabbit anti-extracellular signal-regulated kinases (Erk1/2; cat. no. 4695), rabbit anti-p-Erk1/2 (cat. no. 4377) (all 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and rabbit anti-β-actin (cat. no. sc-7210; 1:4,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing, Knock-Out, Western Blot

    Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

    doi: 10.3892/etm.2018.5783

    Figure Lengend Snippet: Molecular mechanisms by which curcumin regulates the IGF-1R signaling pathway and the expression of VEGF in HepG2 cells under CoCl 2 -induced hypoxia. The arrow and blocked arrow (no arrowhead) indicate stimulation and inhibition, respectively. The dotted arrow indicates the translocation of HIF-1α and HIF-1β. The dotted blocked arrow indicates the potential effects of curcumin not directly examined in the present study. It was concluded that curcumin may suppress the expression of HIF-1 and VEGF by targeting IGF-1R or its downstream signaling pathways. VEGF, vascular endothelial growth factor; HIF-1α, hypoxia-inducible factor-1α; HIF-1β, hypoxia-inducible factor-1β; IGF-1, insulin-like growth factor-1; IGF-1R, insulin-like growth factor-1 receptor; PI3K, phosphoinositide-3-kinase; Akt, protein kinase B; MEK, mitogen-activated protein kinase-Erk kinase; Erk, extracellular signal-regulated kinase.

    Article Snippet: The primary antibodies used in the experiment were as follows: Rabbit anti-IGF-1R (cat. no. 9750), rabbit anti-HIF-1α (cat. no. 3716), rabbit anti-VEGF (cat. no. 2463), rabbit anti-Akt (cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (cat. no. 5012), rabbit anti-extracellular signal-regulated kinases (Erk1/2; cat. no. 4695), rabbit anti-p-Erk1/2 (cat. no. 4377) (all 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and rabbit anti-β-actin (cat. no. sc-7210; 1:4,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing, Inhibition, Translocation Assay

    Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Effect of curcumin on vascular endothelial growth factor in hypoxic HepG2 cells via the insulin-like growth factor 1 receptor signaling pathway

    doi: 10.3892/etm.2018.5783

    Figure Lengend Snippet: Effect of CoCl 2 -induced hypoxia on IGF-1R, HIF-1α and VEGF protein expression in HepG2 cells. (A) Serum-starved IGF-1R knockout HepG2 cells were treated with different concentrations of CoCl 2 (0, 50, 100, 150, 200 and 400 µM) for 6 h. IGF-1R, HIF-1α and VEGF protein expression were detected by western blot analysis. (B) Western blot analysis data was quantified and the protein expression of IGF-1R, HIF-1α and VEGF are presented as a bar graph. *P

    Article Snippet: The primary antibodies used in the experiment were as follows: Rabbit anti-IGF-1R (cat. no. 9750), rabbit anti-HIF-1α (cat. no. 3716), rabbit anti-VEGF (cat. no. 2463), rabbit anti-Akt (cat. no. 9272), rabbit anti-phosphorylated (p)-Akt (cat. no. 5012), rabbit anti-extracellular signal-regulated kinases (Erk1/2; cat. no. 4695), rabbit anti-p-Erk1/2 (cat. no. 4377) (all 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and rabbit anti-β-actin (cat. no. sc-7210; 1:4,000; Santa Cruz Biotechnology, Inc.).

    Techniques: Expressing, Knock-Out, Western Blot

    Gene expression analysis of GEnCs cultured on hydrogel substrates at designated time points. (A)  PECAM1  encoding for platelet endothelial cell adhesion molecule or CD31. (B)  CDH5  encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C)  ITGB1  encoding for integrin subunit β1. (D)  ITGB3  encoding for integrin subunit β3. (E)  KDR  encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F)  TEK  encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G)  VWF  encoding for von Willebrand factor. (H)  NOS3  encoding for nitric oxide synthase 3. (I)  PLVAP  encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression  normalized to gene expression of GEnCs cultured in tissue culture polystyrene on day 0 ( n  = 4).

    Journal: Biomaterials

    Article Title: Poly(Ethylene Glycol)-Crosslinked Gelatin Hydrogel Substrates with Conjugated Bioactive Peptides Influence Endothelial Cell Behavior

    doi: 10.1016/j.biomaterials.2019.02.001

    Figure Lengend Snippet: Gene expression analysis of GEnCs cultured on hydrogel substrates at designated time points. (A) PECAM1 encoding for platelet endothelial cell adhesion molecule or CD31. (B) CDH5 encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C) ITGB1 encoding for integrin subunit β1. (D) ITGB3 encoding for integrin subunit β3. (E) KDR encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F) TEK encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G) VWF encoding for von Willebrand factor. (H) NOS3 encoding for nitric oxide synthase 3. (I) PLVAP encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression normalized to gene expression of GEnCs cultured in tissue culture polystyrene on day 0 ( n = 4).

    Article Snippet: Primary antibodies were diluted in Sea Block as follows: mouse anti-PECAM-1 at 1:100 (Abcam, #ab187377) and rabbit anti-VEGF Receptor 2 at 1:200 (Cell Signaling Technology, #2479).

    Techniques: Expressing, Cell Culture

    Gene expression analysis and immunofluorescence staining of HUVECs cultured on hydrogel substrates at designated time points. (A)  PECAM1  encoding for platelet endothelial cell adhesion molecule or CD31. (B)  CDH5  encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C)  ITGB1  encoding for integrin subunit β1. (D)  ITGB3  encoding for integrin subunit β3. (E)  KDR  encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F)  TEK  encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G)  VWF  encoding for von Willebrand factor. (H)  NOS3  encoding for nitric oxide synthase 3. (I)  PLVAP  encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression normalized to gene expression of HUVECs cultured in tissue culture polystyrene on day 0 ( n  = 4). (J) Whole-mount immunofluorescence staining of HUVECs cultured on hydrogel substrates at 6 and 24 hrs. Merged images: PECAM-1 (green), VEGFR2 (red), and DAPI (blue).

    Journal: Biomaterials

    Article Title: Poly(Ethylene Glycol)-Crosslinked Gelatin Hydrogel Substrates with Conjugated Bioactive Peptides Influence Endothelial Cell Behavior

    doi: 10.1016/j.biomaterials.2019.02.001

    Figure Lengend Snippet: Gene expression analysis and immunofluorescence staining of HUVECs cultured on hydrogel substrates at designated time points. (A) PECAM1 encoding for platelet endothelial cell adhesion molecule or CD31. (B) CDH5 encoding for cadherin 5 or vascular endothelial cadherin, also known as CD144. (C) ITGB1 encoding for integrin subunit β1. (D) ITGB3 encoding for integrin subunit β3. (E) KDR encoding for kinase insert domain receptor or vascular endothelial growth factor receptor 2. (F) TEK encoding for tyrosine kinase with immunoglobulin-like and EGF-like domains 2 or TIE2. (G) VWF encoding for von Willebrand factor. (H) NOS3 encoding for nitric oxide synthase 3. (I) PLVAP encoding for plasmalemma vesicle-associated protein. Values presented as fold-change expression normalized to gene expression of HUVECs cultured in tissue culture polystyrene on day 0 ( n = 4). (J) Whole-mount immunofluorescence staining of HUVECs cultured on hydrogel substrates at 6 and 24 hrs. Merged images: PECAM-1 (green), VEGFR2 (red), and DAPI (blue).

    Article Snippet: Primary antibodies were diluted in Sea Block as follows: mouse anti-PECAM-1 at 1:100 (Abcam, #ab187377) and rabbit anti-VEGF Receptor 2 at 1:200 (Cell Signaling Technology, #2479).

    Techniques: Expressing, Immunofluorescence, Staining, Cell Culture

    Effect of scutellarin and scutellarin-loaded nanoparticles on the expression of VEGF, VEGFR2 and vWF. a Western blot analysis was used to determine the expression of VEGF,VEGFR2 and vWF in the rat retina. Quantitative evaluation of protein expression of VEGF/GAPDH ( b ), VEGFR2/GAPDH ( c ) and vWF/GAPDH ( d ). Data are presented as mean ± SD. *Significantly different from control group ( P

    Journal: Journal of Nanobiotechnology

    Article Title: Enhancement of scutellarin oral delivery efficacy by vitamin B12-modified amphiphilic chitosan derivatives to treat type II diabetes induced-retinopathy

    doi: 10.1186/s12951-017-0251-z

    Figure Lengend Snippet: Effect of scutellarin and scutellarin-loaded nanoparticles on the expression of VEGF, VEGFR2 and vWF. a Western blot analysis was used to determine the expression of VEGF,VEGFR2 and vWF in the rat retina. Quantitative evaluation of protein expression of VEGF/GAPDH ( b ), VEGFR2/GAPDH ( c ) and vWF/GAPDH ( d ). Data are presented as mean ± SD. *Significantly different from control group ( P

    Article Snippet: Primary antibodies against VEGF, VEGFR2, vWF and Horseradish peroxidase (HRP)-conjugated secondary antibodies were obtained from Cell Signaling Technology (Beverly, MA).

    Techniques: Expressing, Western Blot

    Bevacizumab treatment concurrent with nab-paclitaxel chemotherapy is the most effective regimen for inhibiting tumor growth and metastasis. (A, B) Mice bearing 435-LuC + tumors of 450 mm 3 in volume were treated with bevacizumab (4 mg/kg) using three different combination regimens with nab-paclitaxel (10 mg/kg, three cycles of daily for five consecutive days). (A) Schematic illustration of experimental design describing different regimens assessed in this study. Group 1 received three cycles of nab-paclitaxel followed by bevacizumab for the duration of the study, group 2 received bevacizumab concurrent with nab-paclitaxel and both drugs were discontinued at the end of three cycles of nab-paclitaxel, and group 3 received bevacizumab throughout three cycles of nab-paclitaxel and for the duration of the study. (B) Black arrows indicate the start of each cycle of nab-paclitaxel treatment. Data are presented as the mean tumor volume ± SE per group at the indicated days after implantation. Frozen sections from control- (C, top) and nab-paclitaxel-treated (C, bottom) tumors were stained with anti-VEGF-A antibodies and counterstained with 4′,6-diamidino-2-phenylindole to visualize the nuclei. Images were acquired at a constant exposure setting and total fluorescent intensity of VEGF-A staining from control (D, top) and treated (D, bottom) tumor sections was plotted using the ImageJ software as described in the Materials and Methods. Fluorescent intensity plots of representative images in (C) are shown. All images were acquired at a magnification of x200.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Synergy of Nab-paclitaxel and Bevacizumab in Eradicating Large Orthotopic Breast Tumors and Preexisting Metastases 1Synergy of Nab-paclitaxel and Bevacizumab in Eradicating Large Orthotopic Breast Tumors and Preexisting Metastases 1 2

    doi:

    Figure Lengend Snippet: Bevacizumab treatment concurrent with nab-paclitaxel chemotherapy is the most effective regimen for inhibiting tumor growth and metastasis. (A, B) Mice bearing 435-LuC + tumors of 450 mm 3 in volume were treated with bevacizumab (4 mg/kg) using three different combination regimens with nab-paclitaxel (10 mg/kg, three cycles of daily for five consecutive days). (A) Schematic illustration of experimental design describing different regimens assessed in this study. Group 1 received three cycles of nab-paclitaxel followed by bevacizumab for the duration of the study, group 2 received bevacizumab concurrent with nab-paclitaxel and both drugs were discontinued at the end of three cycles of nab-paclitaxel, and group 3 received bevacizumab throughout three cycles of nab-paclitaxel and for the duration of the study. (B) Black arrows indicate the start of each cycle of nab-paclitaxel treatment. Data are presented as the mean tumor volume ± SE per group at the indicated days after implantation. Frozen sections from control- (C, top) and nab-paclitaxel-treated (C, bottom) tumors were stained with anti-VEGF-A antibodies and counterstained with 4′,6-diamidino-2-phenylindole to visualize the nuclei. Images were acquired at a constant exposure setting and total fluorescent intensity of VEGF-A staining from control (D, top) and treated (D, bottom) tumor sections was plotted using the ImageJ software as described in the Materials and Methods. Fluorescent intensity plots of representative images in (C) are shown. All images were acquired at a magnification of x200.

    Article Snippet: Primary rabbit anti-Bcl-xL, anti-Akt, anti-p-Akt, anti-p44/42, anti-p-p44/42 anti-p50, anti-p-p50, anti-p65, anti-p p65, anti-VEGF-A, and anti-Bcl-2 antibodies were from Cell Signaling (Danvers, MA) and Thermo Scientific (Waltham, MA).

    Techniques: Mouse Assay, Staining, Software