Structured Review

Proteintech anti upf1 antibody
(A) Correlation of <t>UPF1</t> expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.
Anti Upf1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti upf1 antibody/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti upf1 antibody - by Bioz Stars, 2024-05
86/100 stars

Images

1) Product Images from "Widespread 3’ UTR splicing regulates expression of oncogene transcripts in sequence-dependent and independent manners"

Article Title: Widespread 3’ UTR splicing regulates expression of oncogene transcripts in sequence-dependent and independent manners

Journal: bioRxiv

doi: 10.1101/2024.01.10.575007

(A) Correlation of UPF1 expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.
Figure Legend Snippet: (A) Correlation of UPF1 expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.

Techniques Used: Expressing, Negative Control, Construct, Transfection, Comparison

(A) Comparison of splicing of pPTC and nonPTC 3UIs between normal and cancer samples in a variety of tissue types from TCGA. Orange bars represent the percentage of transcripts that are spliced more in cancer compared to normal. Grey bars represent the percentage of transcripts that are retained more in cancer compared to normal. (B) Distribution of significant intron inclusion events between normal and cancerous colon tissue. Grey dots represent significant events (padj < 0.05) with no effect-size threshold. Orange dots represent significant events with IncLevelDifference > 5%. Positive IncLevelDifference means more retention occurs in colon cancer, negative values mean more splicing occurs in colon cancer. (C) K-means clustering of PSO values for events that were significantly different between normal and cancer samples. (D) Percentage of events in each cluster that were upregulated (Rescue) or downregulated (Sensitising) upon UPF1 knockdown. (E-F) Gene set enrichment analysis for events which are differentially regulated between normal and colon cancer samples: (E) Apoptosis from the MSigDB Hallmarks Collection; (F) Canonical Wnt Signalling Pathway from MSigDB C5 Collection.
Figure Legend Snippet: (A) Comparison of splicing of pPTC and nonPTC 3UIs between normal and cancer samples in a variety of tissue types from TCGA. Orange bars represent the percentage of transcripts that are spliced more in cancer compared to normal. Grey bars represent the percentage of transcripts that are retained more in cancer compared to normal. (B) Distribution of significant intron inclusion events between normal and cancerous colon tissue. Grey dots represent significant events (padj < 0.05) with no effect-size threshold. Orange dots represent significant events with IncLevelDifference > 5%. Positive IncLevelDifference means more retention occurs in colon cancer, negative values mean more splicing occurs in colon cancer. (C) K-means clustering of PSO values for events that were significantly different between normal and cancer samples. (D) Percentage of events in each cluster that were upregulated (Rescue) or downregulated (Sensitising) upon UPF1 knockdown. (E-F) Gene set enrichment analysis for events which are differentially regulated between normal and colon cancer samples: (E) Apoptosis from the MSigDB Hallmarks Collection; (F) Canonical Wnt Signalling Pathway from MSigDB C5 Collection.

Techniques Used: Comparison


Structured Review

Proteintech anti upf1
Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti upf1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti upf1 - by Bioz Stars, 2024-05
86/100 stars

Images


Structured Review

Proteintech anti upf1
Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti upf1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti upf1 - by Bioz Stars, 2024-05
86/100 stars

Images


Structured Review

Proteintech anti upf1
Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti upf1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti upf1 - by Bioz Stars, 2024-05
86/100 stars

Images


Structured Review

Proteintech rabbit anti upf1
Rabbit Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti upf1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti upf1 - by Bioz Stars, 2024-05
86/100 stars

Images


Structured Review

Proteintech upf1
Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/upf1/product/Proteintech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
upf1 - by Bioz Stars, 2024-05
86/100 stars

Images


Structured Review

Proteintech anti upf1 antibody
<t>Bmi1-UPF1-HK2</t> pathway promotes aerobic glycolysis in PC cells. ( A ) Western blot of Bmi1, SLC2A1, HK2, PKM2, and LDHA protein in PC cells transfected with vector or Bmi1 plasmid. ( B ) Western blot of Bmi1 and HK2 protein in PC cells after transfection with ncRNA or Bmi1 siRNA under different glycemic conditions. ( C ) The GEPIA web tool was adopted to search for the correlation between the expression of Bmi1 and UPF1 in mRNA level in pancreatic ductal adenocarcinoma samples. P values as indicated in the Figure. ( D ) The GEPIA web tool was adopted to search for the correlation between the expression of UPF1 and HK2 at mRNA levelsin pancreatic ductal adenocarcinoma samples. P- values as indicated in the Figure. ( E ) Western blot of Bmi1 and UPF1 protein in PC cells transfected with vector or Bmi1 plasmid. ( F ) Western blot of Bmi1, UPF1, and HK2 protein in sh-control or sh-UPF1 PC cells transfected with vector or Bmi1 plasmid. ( G ) RNA immunoprecipitation (RIP) assay was used to detect the binding between UPF1 and HK2 mRNA. ( H ) HK2 mRNA half-life measured by qRT-PCR after actinomycin D treatment. ( I ) Bar graphs depicting lactate production with or without 2-DG (5 mM) in PC cells after transfection with ncRNA or Bmi1 oeRNA. Data presented in the graphs represented means ± standard deviation from 3 parallel experiments. ( J ) Bar graphs depicting flow cytometric analysis of activated cytotoxic T-cell (INFγ + and CD8 + ) population in co-culture of primary T-cells and PC cells transfected with vector or Bmi1 plasmid pretreated with or without 2-DG (5 mM). ( K ) Bar graphs depicting the levels of soluble IL-2 of Jurkat T-cells with the conditional medium of PC cells transfected with vector or Bmi1 plasmid pretreated with or without 2-DG (5 mM). ∗∗∗ P < .001; ∗∗ P < .01; ∗ P < .05.
Anti Upf1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti upf1 antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti upf1 antibody - by Bioz Stars, 2024-05
93/100 stars

Images

1) Product Images from "Hyperglycemia Enhances Immunosuppression and Aerobic Glycolysis of Pancreatic Cancer Through Upregulating Bmi1-UPF1-HK2 Pathway"

Article Title: Hyperglycemia Enhances Immunosuppression and Aerobic Glycolysis of Pancreatic Cancer Through Upregulating Bmi1-UPF1-HK2 Pathway

Journal: Cellular and Molecular Gastroenterology and Hepatology

doi: 10.1016/j.jcmgh.2022.07.008

Bmi1-UPF1-HK2 pathway promotes aerobic glycolysis in PC cells. ( A ) Western blot of Bmi1, SLC2A1, HK2, PKM2, and LDHA protein in PC cells transfected with vector or Bmi1 plasmid. ( B ) Western blot of Bmi1 and HK2 protein in PC cells after transfection with ncRNA or Bmi1 siRNA under different glycemic conditions. ( C ) The GEPIA web tool was adopted to search for the correlation between the expression of Bmi1 and UPF1 in mRNA level in pancreatic ductal adenocarcinoma samples. P values as indicated in the Figure. ( D ) The GEPIA web tool was adopted to search for the correlation between the expression of UPF1 and HK2 at mRNA levelsin pancreatic ductal adenocarcinoma samples. P- values as indicated in the Figure. ( E ) Western blot of Bmi1 and UPF1 protein in PC cells transfected with vector or Bmi1 plasmid. ( F ) Western blot of Bmi1, UPF1, and HK2 protein in sh-control or sh-UPF1 PC cells transfected with vector or Bmi1 plasmid. ( G ) RNA immunoprecipitation (RIP) assay was used to detect the binding between UPF1 and HK2 mRNA. ( H ) HK2 mRNA half-life measured by qRT-PCR after actinomycin D treatment. ( I ) Bar graphs depicting lactate production with or without 2-DG (5 mM) in PC cells after transfection with ncRNA or Bmi1 oeRNA. Data presented in the graphs represented means ± standard deviation from 3 parallel experiments. ( J ) Bar graphs depicting flow cytometric analysis of activated cytotoxic T-cell (INFγ + and CD8 + ) population in co-culture of primary T-cells and PC cells transfected with vector or Bmi1 plasmid pretreated with or without 2-DG (5 mM). ( K ) Bar graphs depicting the levels of soluble IL-2 of Jurkat T-cells with the conditional medium of PC cells transfected with vector or Bmi1 plasmid pretreated with or without 2-DG (5 mM). ∗∗∗ P < .001; ∗∗ P < .01; ∗ P < .05.
Figure Legend Snippet: Bmi1-UPF1-HK2 pathway promotes aerobic glycolysis in PC cells. ( A ) Western blot of Bmi1, SLC2A1, HK2, PKM2, and LDHA protein in PC cells transfected with vector or Bmi1 plasmid. ( B ) Western blot of Bmi1 and HK2 protein in PC cells after transfection with ncRNA or Bmi1 siRNA under different glycemic conditions. ( C ) The GEPIA web tool was adopted to search for the correlation between the expression of Bmi1 and UPF1 in mRNA level in pancreatic ductal adenocarcinoma samples. P values as indicated in the Figure. ( D ) The GEPIA web tool was adopted to search for the correlation between the expression of UPF1 and HK2 at mRNA levelsin pancreatic ductal adenocarcinoma samples. P- values as indicated in the Figure. ( E ) Western blot of Bmi1 and UPF1 protein in PC cells transfected with vector or Bmi1 plasmid. ( F ) Western blot of Bmi1, UPF1, and HK2 protein in sh-control or sh-UPF1 PC cells transfected with vector or Bmi1 plasmid. ( G ) RNA immunoprecipitation (RIP) assay was used to detect the binding between UPF1 and HK2 mRNA. ( H ) HK2 mRNA half-life measured by qRT-PCR after actinomycin D treatment. ( I ) Bar graphs depicting lactate production with or without 2-DG (5 mM) in PC cells after transfection with ncRNA or Bmi1 oeRNA. Data presented in the graphs represented means ± standard deviation from 3 parallel experiments. ( J ) Bar graphs depicting flow cytometric analysis of activated cytotoxic T-cell (INFγ + and CD8 + ) population in co-culture of primary T-cells and PC cells transfected with vector or Bmi1 plasmid pretreated with or without 2-DG (5 mM). ( K ) Bar graphs depicting the levels of soluble IL-2 of Jurkat T-cells with the conditional medium of PC cells transfected with vector or Bmi1 plasmid pretreated with or without 2-DG (5 mM). ∗∗∗ P < .001; ∗∗ P < .01; ∗ P < .05.

Techniques Used: Western Blot, Transfection, Plasmid Preparation, Expressing, Immunoprecipitation, Binding Assay, Quantitative RT-PCR, Standard Deviation, Co-Culture Assay

The role of hyperglycemia-induced Bmi1 expression under immunodeficient environment. ( A ) Experimental schema of generating the immunodeficient BALB/c nude mice model; and alterations in blood glucose levels after STZ treatment. ( B ) Representative pictures of the orthotopic tumors. ( C ) Changes in tumor volume and tumor weight in different groups. . ( D ) The lactic acid concentration and glucose concentration of tumor tissues from normal and diabetes animal models. ( E ) IHC staining to evaluate the ACLY, KAT4, H4ac, and Bmi1 protein level in euglycemia-NC and hyperglycemia-NC. ( F ) IHC staining to evaluate Bmi1, UPF1, and HK2 protein level in KD-Bmi1, NC, and OE-Bmi1 in the euglycemia model. ( G ) Schematic diagram showing the effect of the hyperglycemia-Bmi1-HK2 pathway on the Warburg effect and TME. Data presented in the graphs represented means ± standard deviation from 3 parallel experiments. ∗∗∗∗ P < .0001; ∗∗∗ P < .001; ∗∗ P < .01; ∗ P < .05.
Figure Legend Snippet: The role of hyperglycemia-induced Bmi1 expression under immunodeficient environment. ( A ) Experimental schema of generating the immunodeficient BALB/c nude mice model; and alterations in blood glucose levels after STZ treatment. ( B ) Representative pictures of the orthotopic tumors. ( C ) Changes in tumor volume and tumor weight in different groups. . ( D ) The lactic acid concentration and glucose concentration of tumor tissues from normal and diabetes animal models. ( E ) IHC staining to evaluate the ACLY, KAT4, H4ac, and Bmi1 protein level in euglycemia-NC and hyperglycemia-NC. ( F ) IHC staining to evaluate Bmi1, UPF1, and HK2 protein level in KD-Bmi1, NC, and OE-Bmi1 in the euglycemia model. ( G ) Schematic diagram showing the effect of the hyperglycemia-Bmi1-HK2 pathway on the Warburg effect and TME. Data presented in the graphs represented means ± standard deviation from 3 parallel experiments. ∗∗∗∗ P < .0001; ∗∗∗ P < .001; ∗∗ P < .01; ∗ P < .05.

Techniques Used: Expressing, Concentration Assay, Immunohistochemistry, Standard Deviation


Structured Review

Proteintech upf1 primary antibody
The tRF-Leu-AAG promoted PC development by suppressing <t>UPF1.</t>
Upf1 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/upf1 primary antibody/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
upf1 primary antibody - by Bioz Stars, 2024-05
93/100 stars

Images

1) Product Images from "The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells"

Article Title: The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells

Journal: Bioengineered

doi: 10.1080/21655979.2022.2064206

The tRF-Leu-AAG promoted PC development by suppressing UPF1.
Figure Legend Snippet: The tRF-Leu-AAG promoted PC development by suppressing UPF1.

Techniques Used:


Structured Review

Proteintech β actin primary antibody
β Actin Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Proteintech rabbit polyclonal anti upf1
BC cell migration and proliferation are regulated by PVT1 and <t>UPF1.</t> A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
Rabbit Polyclonal Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti upf1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti upf1 - by Bioz Stars, 2024-05
93/100 stars

Images

1) Product Images from "Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1"

Article Title: Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1

Journal: Breast Cancer Research : BCR

doi: 10.1186/s13058-021-01491-y

BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
Figure Legend Snippet: BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

Techniques Used: Migration, Western Blot, Transfection

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    Proteintech anti upf1 antibody
    (A) Correlation of <t>UPF1</t> expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.
    Anti Upf1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti upf1 antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti upf1 antibody - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

    86
    Proteintech anti upf1
    (A) Correlation of <t>UPF1</t> expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.
    Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti upf1/product/Proteintech
    Average 86 stars, based on 1 article reviews
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    anti upf1 - by Bioz Stars, 2024-05
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    86
    Proteintech rabbit anti upf1
    (A) Correlation of <t>UPF1</t> expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.
    Rabbit Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti upf1/product/Proteintech
    Average 86 stars, based on 1 article reviews
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    rabbit anti upf1 - by Bioz Stars, 2024-05
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    86
    Proteintech upf1
    (A) Correlation of <t>UPF1</t> expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.
    Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech upf1 primary antibody
    The tRF-Leu-AAG promoted PC development by suppressing <t>UPF1.</t>
    Upf1 Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Proteintech β actin primary antibody
    The tRF-Leu-AAG promoted PC development by suppressing <t>UPF1.</t>
    β Actin Primary Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Proteintech rabbit polyclonal anti upf1
    BC cell migration and proliferation are regulated by PVT1 and <t>UPF1.</t> A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001
    Rabbit Polyclonal Anti Upf1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Correlation of UPF1 expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.

    Journal: bioRxiv

    Article Title: Widespread 3’ UTR splicing regulates expression of oncogene transcripts in sequence-dependent and independent manners

    doi: 10.1101/2024.01.10.575007

    Figure Lengend Snippet: (A) Correlation of UPF1 expression in each sample with its corresponding average 3UI PSO. Strength of correlation was determined by calculating Spearman’s rank correlation coefficient. (B-C) Empirical cumulative distribution function plots comparing the expression changes induced by UPF1 knockdown on: (B) transcripts that are protein coding (black line), nonPTC (blue line) or expected to be NMD sensitive (pPTC transcripts and transcripts annotated as NMD-sensitive; orange line); (C) transcripts that have their terminal 3UI more (orange line) or less (black line) than 55 nucleotides from the termination codon; Kolmogorov-Smirnov test was performed, P<0.05 is considered statistically significant. (D) Differential transcript usage of 3UIs upon UPF1 knockdown. Each dot represents a 3UI-containing transcript. Red dots represent significant differential transcript usage (P<0.05, effect-size > 5%). (E) Sashimi plot comparing retained vs spliced read coverage for HRAS in siUPF1 (orange) and negative control siDSRed (grey). (F) Schematic diagram of Luciferase2-HRAS 3’ UTR reporter plasmids. Ticks and crosses indicate whether each construct is able to be spliced, and which isoforms it produces upon transfection. (G) Relative luminescence comparison between constructs upon transfection into HCT116 cells. (H) Relative stability of HRAS spliced and retained isoforms in HCT116 cells treated with Actinomycin D.

    Article Snippet: Membranes were blocked with 5% milk in TBST for 1 hour at room temperature followed by incubation with 1:500 anti-UPF1 antibody (Proteintech #66898) or 1:5000 anti-Tubulin (Sigma-Aldrich #T6199) in TBST + 5% milk for 2 hours at room temperature.

    Techniques: Expressing, Negative Control, Construct, Transfection, Comparison

    (A) Comparison of splicing of pPTC and nonPTC 3UIs between normal and cancer samples in a variety of tissue types from TCGA. Orange bars represent the percentage of transcripts that are spliced more in cancer compared to normal. Grey bars represent the percentage of transcripts that are retained more in cancer compared to normal. (B) Distribution of significant intron inclusion events between normal and cancerous colon tissue. Grey dots represent significant events (padj < 0.05) with no effect-size threshold. Orange dots represent significant events with IncLevelDifference > 5%. Positive IncLevelDifference means more retention occurs in colon cancer, negative values mean more splicing occurs in colon cancer. (C) K-means clustering of PSO values for events that were significantly different between normal and cancer samples. (D) Percentage of events in each cluster that were upregulated (Rescue) or downregulated (Sensitising) upon UPF1 knockdown. (E-F) Gene set enrichment analysis for events which are differentially regulated between normal and colon cancer samples: (E) Apoptosis from the MSigDB Hallmarks Collection; (F) Canonical Wnt Signalling Pathway from MSigDB C5 Collection.

    Journal: bioRxiv

    Article Title: Widespread 3’ UTR splicing regulates expression of oncogene transcripts in sequence-dependent and independent manners

    doi: 10.1101/2024.01.10.575007

    Figure Lengend Snippet: (A) Comparison of splicing of pPTC and nonPTC 3UIs between normal and cancer samples in a variety of tissue types from TCGA. Orange bars represent the percentage of transcripts that are spliced more in cancer compared to normal. Grey bars represent the percentage of transcripts that are retained more in cancer compared to normal. (B) Distribution of significant intron inclusion events between normal and cancerous colon tissue. Grey dots represent significant events (padj < 0.05) with no effect-size threshold. Orange dots represent significant events with IncLevelDifference > 5%. Positive IncLevelDifference means more retention occurs in colon cancer, negative values mean more splicing occurs in colon cancer. (C) K-means clustering of PSO values for events that were significantly different between normal and cancer samples. (D) Percentage of events in each cluster that were upregulated (Rescue) or downregulated (Sensitising) upon UPF1 knockdown. (E-F) Gene set enrichment analysis for events which are differentially regulated between normal and colon cancer samples: (E) Apoptosis from the MSigDB Hallmarks Collection; (F) Canonical Wnt Signalling Pathway from MSigDB C5 Collection.

    Article Snippet: Membranes were blocked with 5% milk in TBST for 1 hour at room temperature followed by incubation with 1:500 anti-UPF1 antibody (Proteintech #66898) or 1:5000 anti-Tubulin (Sigma-Aldrich #T6199) in TBST + 5% milk for 2 hours at room temperature.

    Techniques: Comparison

    The tRF-Leu-AAG promoted PC development by suppressing UPF1.

    Journal: Bioengineered

    Article Title: The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells

    doi: 10.1080/21655979.2022.2064206

    Figure Lengend Snippet: The tRF-Leu-AAG promoted PC development by suppressing UPF1.

    Article Snippet: Subsequently, the proteins were blocked with a quick-blocking solution for 30 min. UPF1 primary antibody (1:1000; 23,379-1-AP, Proteintech) or β-Actin primary antibody (1:5000; 60,008-1-Lg, Proteintech) was overnight incubated at 4°C.

    Techniques:

    BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

    Journal: Breast Cancer Research : BCR

    Article Title: Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1

    doi: 10.1186/s13058-021-01491-y

    Figure Lengend Snippet: BC cell migration and proliferation are regulated by PVT1 and UPF1. A Western blot assays for UPF1 in Hs578t and MCF-7 cells with PVT1 knockdown. B RIP assays for UPF1 in Hs578t and MCF-7 cells. C Colony formation of Hs578t cells transfected with si-PVT1, sh-UPF1, or both. D Wound healing assays for Hs578t cells. E Transwell assays for Hs578t cells. F Western blot assays for proteins in Hs578t cells. Results were presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** P < 0.001

    Article Snippet: The primary antibodies were: rabbit monoclonal anti-E-cadherin (#3195, 1:1000, Cell Signaling Technology), rabbit monoclonal anti-Vimentin (#5741, 1:1000, Cell Signaling Technology), rabbit polyclonal anti-UPF1 (23379-1-AP, 1:1000, ProteinTech), rabbit polyclonal anti-FOXQ1 (23718-1-AP, 1:1000, ProteinTech), mouse monoclonal anti-β-actin (A5316, 1:5000, Sigma-Aldrich), and mouse monoclonal anti-PCNA (sc-25280, 1:1000, Santa Cruz).

    Techniques: Migration, Western Blot, Transfection