Journal: PLoS ONE

Article Title: Nuclear Translocation of β-Catenin during Mesenchymal Stem Cells Differentiation into Hepatocytes Is Associated with a Tumoral Phenotype

doi: 10.1371/journal.pone.0034656

Figure Lengend Snippet: Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase β chain (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.

Article Snippet: Other primary antibodies used were: anti-p53 (1∶500) and anti-L-lactate dehydrogenase β chain (1∶200) from Santa Cruz Biotechnologies, anti-tropomyosin β chain (1∶500), adenine phosphoribosyltransferase (1∶500) and Transgelin (1∶2000) that were purchased from Novus Biologicals Littleton, CO, cathepsin B (4 µg/ml) from Sigma-Aldrich and tubulin 1∶10000 from Abcam (Cambridge, UK) were performed.

Techniques: Western Blot

Journal: PLoS ONE

Article Title: Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6

doi: 10.1371/journal.pone.0020389

Figure Lengend Snippet: Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), TPM1 (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.

Article Snippet: Secondary antibodies conjugated to horseradish peroxidase, anti-laminin, anti-calponin 1 (CNN1), anti-GATA6, anti-tropomyosin 1 (TPM1) antibodies, and non fat dry milk were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Real-time Polymerase Chain Reaction, Transfection

Journal: PLoS ONE

Article Title: Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6

doi: 10.1371/journal.pone.0020389

Figure Lengend Snippet: Cells were pre-treated with SKI-2 45 min before being incubated with DMEM for 48 h with TGFβ and analyzed by A) Real-Time PCR analysis B) Western blot analysis and C) Confocal microscopy analysis (63× magnification) using anti fluorescein-conjugated specific antibodies. D) Cells were transfected with scrambled-, SphK1-, SphK2-siRNA, stimulated with TGFβ and analyzed by Western blot analysis for CNN1. Lower panel represents densitometric quantification. E) Cells were pre-treated with MK571 45 min in DMEM before being incubated with TGFβ for 48 h and analyzed by Western blot analysis for TPM1. Lower panel represents densitometric quantification.

Article Snippet: Secondary antibodies conjugated to horseradish peroxidase, anti-laminin, anti-calponin 1 (CNN1), anti-GATA6, anti-tropomyosin 1 (TPM1) antibodies, and non fat dry milk were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Incubation, Real-time Polymerase Chain Reaction, Western Blot, Confocal Microscopy, Transfection

Journal: Biomedicines

Article Title: Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice

doi: 10.3390/biomedicines10102529

Figure Lengend Snippet: Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.

Article Snippet: The antibodies used in this study are; brain-derived neurotrophic factor(BDNF), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α), protein kinase B (pAkt, Akt), tropomyosin receptor kinase B (TrkB), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), extracellular signal-regulated kinases 1/2 (pERK, ERK) (Santa Cruz Biotechnology, CA, USA, 1:200), 4-Hydroxynonena (4HNE) (BD Biosciences, NJ, USA, 1:500), C-Reactive Protein (CRP), irisin, insulin-like growth factor 1 (IGF-1) (Abcam, Cambridge, MA, USA, 1:1000), and α-tubulin (Sigma-Aldrich, St. Louis, MO, USA, 1:4000).

Techniques: Derivative Assay

Journal: Biomedicines

Article Title: Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice

doi: 10.3390/biomedicines10102529

Figure Lengend Snippet: Effects of resistant exercise on hippocampal ( a ) cognitive function-related myokines and ( b ) energy metabolism and ( c ) oxidative stress and inflammation in sarcopenic obese mice. Values are means ± SEM ( n = 5~6). Mean values with the same superscript letter (a and b) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2, 4HNE: 4-Hydroxynonenal, TNF-α: tumor necrosis factor-alpha, CRP: C-Reactive Protein. N.S.: no significance.

Article Snippet: The antibodies used in this study are; brain-derived neurotrophic factor(BDNF), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α), protein kinase B (pAkt, Akt), tropomyosin receptor kinase B (TrkB), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), extracellular signal-regulated kinases 1/2 (pERK, ERK) (Santa Cruz Biotechnology, CA, USA, 1:200), 4-Hydroxynonena (4HNE) (BD Biosciences, NJ, USA, 1:500), C-Reactive Protein (CRP), irisin, insulin-like growth factor 1 (IGF-1) (Abcam, Cambridge, MA, USA, 1:1000), and α-tubulin (Sigma-Aldrich, St. Louis, MO, USA, 1:4000).

Techniques: Derivative Assay