Structured Review

Santa Cruz Biotechnology goat anti human tropomyosin polyclonal antibody
Goat Anti Human Tropomyosin Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Santa Cruz Biotechnology mouse anti tropomyosin ch1
Mouse Anti Tropomyosin Ch1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Santa Cruz Biotechnology anti tropomyosin β chain
Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
Anti Tropomyosin β Chain, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti tropomyosin β chain/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
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anti tropomyosin β chain - by Bioz Stars, 2023-02
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Images

1) Product Images from "Nuclear Translocation of β-Catenin during Mesenchymal Stem Cells Differentiation into Hepatocytes Is Associated with a Tumoral Phenotype"

Article Title: Nuclear Translocation of β-Catenin during Mesenchymal Stem Cells Differentiation into Hepatocytes Is Associated with a Tumoral Phenotype

Journal: PLoS ONE

doi: 10.1371/journal.pone.0034656

Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase β chain (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
Figure Legend Snippet: Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase β chain (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.

Techniques Used: Western Blot


Structured Review

Santa Cruz Biotechnology anti hmw tropomyosin
Anti Hmw Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Structured Review

Santa Cruz Biotechnology antibodies against tropomyosin
Antibodies Against Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology tropomyosin
Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tropomyosin/product/Santa Cruz Biotechnology
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tropomyosin - by Bioz Stars, 2023-02
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Santa Cruz Biotechnology mouse anti tropomyosin
Mouse Anti Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti tropomyosin/product/Santa Cruz Biotechnology
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Santa Cruz Biotechnology rabbit anti tropomyosin
Rabbit Anti Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti tropomyosin/product/Santa Cruz Biotechnology
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Santa Cruz Biotechnology anti tropomyosin 1 tpm1 antibodies
Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), <t>TPM1</t> (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.
Anti Tropomyosin 1 Tpm1 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti tropomyosin 1 tpm1 antibodies/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti tropomyosin 1 tpm1 antibodies - by Bioz Stars, 2023-02
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Images

1) Product Images from "Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6"

Article Title: Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6

Journal: PLoS ONE

doi: 10.1371/journal.pone.0020389

Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), TPM1 (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.
Figure Legend Snippet: Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), TPM1 (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.

Techniques Used: Real-time Polymerase Chain Reaction, Transfection

Cells were pre-treated with SKI-2 45 min before being incubated with DMEM for 48 h with TGFβ and analyzed by A) Real-Time PCR analysis B) Western blot analysis and C) Confocal microscopy analysis (63× magnification) using anti fluorescein-conjugated specific antibodies. D) Cells were transfected with scrambled-, SphK1-, SphK2-siRNA, stimulated with TGFβ and analyzed by Western blot analysis for CNN1. Lower panel represents densitometric quantification. E) Cells were pre-treated with MK571 45 min in DMEM before being incubated with TGFβ for 48 h and analyzed by Western blot analysis for TPM1. Lower panel represents densitometric quantification.
Figure Legend Snippet: Cells were pre-treated with SKI-2 45 min before being incubated with DMEM for 48 h with TGFβ and analyzed by A) Real-Time PCR analysis B) Western blot analysis and C) Confocal microscopy analysis (63× magnification) using anti fluorescein-conjugated specific antibodies. D) Cells were transfected with scrambled-, SphK1-, SphK2-siRNA, stimulated with TGFβ and analyzed by Western blot analysis for CNN1. Lower panel represents densitometric quantification. E) Cells were pre-treated with MK571 45 min in DMEM before being incubated with TGFβ for 48 h and analyzed by Western blot analysis for TPM1. Lower panel represents densitometric quantification.

Techniques Used: Incubation, Real-time Polymerase Chain Reaction, Western Blot, Confocal Microscopy, Transfection


Structured Review

Santa Cruz Biotechnology tropomyosin receptor kinase b trkb
Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.
Tropomyosin Receptor Kinase B Trkb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tropomyosin receptor kinase b trkb/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
tropomyosin receptor kinase b trkb - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice"

Article Title: Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice

Journal: Biomedicines

doi: 10.3390/biomedicines10102529

Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.
Figure Legend Snippet: Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.

Techniques Used: Derivative Assay

Effects of resistant exercise on hippocampal ( a ) cognitive function-related myokines and ( b ) energy metabolism and ( c ) oxidative stress and inflammation in sarcopenic obese mice. Values are means ± SEM ( n = 5~6). Mean values with the same superscript letter (a and b) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2, 4HNE: 4-Hydroxynonenal, TNF-α: tumor necrosis factor-alpha, CRP: C-Reactive Protein. N.S.: no significance.
Figure Legend Snippet: Effects of resistant exercise on hippocampal ( a ) cognitive function-related myokines and ( b ) energy metabolism and ( c ) oxidative stress and inflammation in sarcopenic obese mice. Values are means ± SEM ( n = 5~6). Mean values with the same superscript letter (a and b) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2, 4HNE: 4-Hydroxynonenal, TNF-α: tumor necrosis factor-alpha, CRP: C-Reactive Protein. N.S.: no significance.

Techniques Used: Derivative Assay

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    Santa Cruz Biotechnology goat anti human tropomyosin polyclonal antibody
    Goat Anti Human Tropomyosin Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti human tropomyosin polyclonal antibody/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
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    Santa Cruz Biotechnology mouse anti tropomyosin ch1
    Mouse Anti Tropomyosin Ch1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti tropomyosin β chain
    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
    Anti Tropomyosin β Chain, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tropomyosin β chain/product/Santa Cruz Biotechnology
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    Santa Cruz Biotechnology anti hmw tropomyosin
    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
    Anti Hmw Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology antibodies against tropomyosin
    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
    Antibodies Against Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology tropomyosin
    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
    Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti tropomyosin
    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
    Mouse Anti Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti tropomyosin
    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase <t>β</t> <t>chain</t> (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.
    Rabbit Anti Tropomyosin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti tropomyosin 1 tpm1 antibodies
    Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), <t>TPM1</t> (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.
    Anti Tropomyosin 1 Tpm1 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology tropomyosin receptor kinase b trkb
    Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.
    Tropomyosin Receptor Kinase B Trkb, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase β chain (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.

    Journal: PLoS ONE

    Article Title: Nuclear Translocation of β-Catenin during Mesenchymal Stem Cells Differentiation into Hepatocytes Is Associated with a Tumoral Phenotype

    doi: 10.1371/journal.pone.0034656

    Figure Lengend Snippet: Relative abundance of specific proteins (DIGE analysis) in human mesenchymal stem cells undifferentiated after 21 days of culture (UC21d) and in mesenchymal stem cells differentiated into hepatocytes with conditioned medium 1 (CM1) or 2 (CM2). B) Western blot confirmation of the changes observed by DIGE analysis in the abundance of some proteins in CM1 and CM2 hepatocytes: Adenine phosphoriobosyl transferase (APT), cathepsin B precursor (CATB), L-lactate dehydrogenase β chain (LDHB), transgelin (TGL2), tropomyosin β chain (TPM2) and nuclear β-catenin. Tubulin and TFIIB were used as cytoplasm and nuclear loading control respectively.

    Article Snippet: Other primary antibodies used were: anti-p53 (1∶500) and anti-L-lactate dehydrogenase β chain (1∶200) from Santa Cruz Biotechnologies, anti-tropomyosin β chain (1∶500), adenine phosphoribosyltransferase (1∶500) and Transgelin (1∶2000) that were purchased from Novus Biologicals Littleton, CO, cathepsin B (4 µg/ml) from Sigma-Aldrich and tubulin 1∶10000 from Abcam (Cambridge, UK) were performed.

    Techniques: Western Blot

    Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), TPM1 (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.

    Journal: PLoS ONE

    Article Title: Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6

    doi: 10.1371/journal.pone.0020389

    Figure Lengend Snippet: Mutual regulation of the transcription factors GATA6, LMCD1 and TRPS1 (D,E,F). Real time PCR analysis of GATA6 (A) was performed in cells challenged with the indicated concentrations of S1P for 6 h. Real-Time PCR analysis of CNN1 (B), TPM1 (C), GATA6 (D), LMCD1 (E), TRPS1 (F) was performed in cells transfected with scrambled-, GATA6- or LMCD1-siRNA and stimulated with S1P for 24 h.

    Article Snippet: Secondary antibodies conjugated to horseradish peroxidase, anti-laminin, anti-calponin 1 (CNN1), anti-GATA6, anti-tropomyosin 1 (TPM1) antibodies, and non fat dry milk were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction, Transfection

    Cells were pre-treated with SKI-2 45 min before being incubated with DMEM for 48 h with TGFβ and analyzed by A) Real-Time PCR analysis B) Western blot analysis and C) Confocal microscopy analysis (63× magnification) using anti fluorescein-conjugated specific antibodies. D) Cells were transfected with scrambled-, SphK1-, SphK2-siRNA, stimulated with TGFβ and analyzed by Western blot analysis for CNN1. Lower panel represents densitometric quantification. E) Cells were pre-treated with MK571 45 min in DMEM before being incubated with TGFβ for 48 h and analyzed by Western blot analysis for TPM1. Lower panel represents densitometric quantification.

    Journal: PLoS ONE

    Article Title: Sphingosine 1-Phosphate Induces Differentiation of Mesoangioblasts towards Smooth Muscle. A Role for GATA6

    doi: 10.1371/journal.pone.0020389

    Figure Lengend Snippet: Cells were pre-treated with SKI-2 45 min before being incubated with DMEM for 48 h with TGFβ and analyzed by A) Real-Time PCR analysis B) Western blot analysis and C) Confocal microscopy analysis (63× magnification) using anti fluorescein-conjugated specific antibodies. D) Cells were transfected with scrambled-, SphK1-, SphK2-siRNA, stimulated with TGFβ and analyzed by Western blot analysis for CNN1. Lower panel represents densitometric quantification. E) Cells were pre-treated with MK571 45 min in DMEM before being incubated with TGFβ for 48 h and analyzed by Western blot analysis for TPM1. Lower panel represents densitometric quantification.

    Article Snippet: Secondary antibodies conjugated to horseradish peroxidase, anti-laminin, anti-calponin 1 (CNN1), anti-GATA6, anti-tropomyosin 1 (TPM1) antibodies, and non fat dry milk were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

    Techniques: Incubation, Real-time Polymerase Chain Reaction, Western Blot, Confocal Microscopy, Transfection

    Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.

    Journal: Biomedicines

    Article Title: Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice

    doi: 10.3390/biomedicines10102529

    Figure Lengend Snippet: Effects of resistant exercise on skeletal muscle. ( a ) cognitive function-related myokines and ( b ) energy metabolism in sarcopenic obese mice. Values are means ± SD ( n = 5~6). Mean values with the same superscript letter (a, b and c) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2. N.S.: no significance.

    Article Snippet: The antibodies used in this study are; brain-derived neurotrophic factor(BDNF), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α), protein kinase B (pAkt, Akt), tropomyosin receptor kinase B (TrkB), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), extracellular signal-regulated kinases 1/2 (pERK, ERK) (Santa Cruz Biotechnology, CA, USA, 1:200), 4-Hydroxynonena (4HNE) (BD Biosciences, NJ, USA, 1:500), C-Reactive Protein (CRP), irisin, insulin-like growth factor 1 (IGF-1) (Abcam, Cambridge, MA, USA, 1:1000), and α-tubulin (Sigma-Aldrich, St. Louis, MO, USA, 1:4000).

    Techniques: Derivative Assay

    Effects of resistant exercise on hippocampal ( a ) cognitive function-related myokines and ( b ) energy metabolism and ( c ) oxidative stress and inflammation in sarcopenic obese mice. Values are means ± SEM ( n = 5~6). Mean values with the same superscript letter (a and b) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2, 4HNE: 4-Hydroxynonenal, TNF-α: tumor necrosis factor-alpha, CRP: C-Reactive Protein. N.S.: no significance.

    Journal: Biomedicines

    Article Title: Potential Effects of Resistant Exercise on Cognitive and Muscle Functions Mediated by Myokines in Sarcopenic Obese Mice

    doi: 10.3390/biomedicines10102529

    Figure Lengend Snippet: Effects of resistant exercise on hippocampal ( a ) cognitive function-related myokines and ( b ) energy metabolism and ( c ) oxidative stress and inflammation in sarcopenic obese mice. Values are means ± SEM ( n = 5~6). Mean values with the same superscript letter (a and b) are not significantly different ( p < 0.05). BDNF: brain-derived neurotrophic factor, Trk-B: tropomyosin receptor kinase B, PGC-1α: peroxisome proliferator-activated receptor-gamma coactivator 1-alpha, CTSB: cathepsin B, IL-6: interleukin-6, IGF-1: insulin-like growth factor 1, Akt: protein kinase B, ERK1/2: extracellular signal-regulated kinases 1/2, 4HNE: 4-Hydroxynonenal, TNF-α: tumor necrosis factor-alpha, CRP: C-Reactive Protein. N.S.: no significance.

    Article Snippet: The antibodies used in this study are; brain-derived neurotrophic factor(BDNF), peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC-1α), protein kinase B (pAkt, Akt), tropomyosin receptor kinase B (TrkB), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), extracellular signal-regulated kinases 1/2 (pERK, ERK) (Santa Cruz Biotechnology, CA, USA, 1:200), 4-Hydroxynonena (4HNE) (BD Biosciences, NJ, USA, 1:500), C-Reactive Protein (CRP), irisin, insulin-like growth factor 1 (IGF-1) (Abcam, Cambridge, MA, USA, 1:1000), and α-tubulin (Sigma-Aldrich, St. Louis, MO, USA, 1:4000).

    Techniques: Derivative Assay