tbx3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tbx3
    Tbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tbx3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tbx3
    ZFHX3 induces the expression of MYC and <t>TBX3</t> in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) Detection of MYC, TBX3, OCT4, and SOX2 by IHC staining in xenograft tumors of T-47D cells ectopically expressing ZFHX3, as shown by representative IHC images (left) and quantification of cells with positive staining (right). Scale bar, 50 µm. ( c , d ) Ectopic expression of ZFHX3 in T-47D cells increased ( c ), while ZFHX3 silencing in MCF-7 cells decreased ( d ), the expression of MYC, TBX3, OCT4, NANOG, and SOX2, as detected by western blotting analysis. ( e ) Estrogen (E 2 ) increased MYC and TBX3 in MCF-7 cells, as detected by western blotting. ( f – i ) ZFHX3 silencing in MCF-7 cells attenuated, while ectopic expression of ZFHX3 in T-47D cells enhanced, E 2 -mediated MYC expression, as detected by western blotting ( f , h ) and quantified by relative MYC ratio ( g , i ). Cells were treated with E 2 for 24 h at the indicated concentrations. siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . ** p < 0.01. ( a , b ) IHC staining was done in xenograft tumors. ( c – i ) cells were cultured in 2D plastic surface and examined with western blotting. Ratios of protein band intensities to those of their loading controls, with the control sample’s normalized to 1, are shown under western blot bands ( c – f , h ). Uncropped western blot images are available in .
    Tbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "ZFHX3 Promotes the Proliferation and Tumor Growth of ER-Positive Breast Cancer Cells Likely by Enhancing Stem-Like Features and MYC and TBX3 Transcription"

    Article Title: ZFHX3 Promotes the Proliferation and Tumor Growth of ER-Positive Breast Cancer Cells Likely by Enhancing Stem-Like Features and MYC and TBX3 Transcription

    Journal: Cancers

    doi: 10.3390/cancers12113415

    ZFHX3 induces the expression of MYC and TBX3 in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) Detection of MYC, TBX3, OCT4, and SOX2 by IHC staining in xenograft tumors of T-47D cells ectopically expressing ZFHX3, as shown by representative IHC images (left) and quantification of cells with positive staining (right). Scale bar, 50 µm. ( c , d ) Ectopic expression of ZFHX3 in T-47D cells increased ( c ), while ZFHX3 silencing in MCF-7 cells decreased ( d ), the expression of MYC, TBX3, OCT4, NANOG, and SOX2, as detected by western blotting analysis. ( e ) Estrogen (E 2 ) increased MYC and TBX3 in MCF-7 cells, as detected by western blotting. ( f – i ) ZFHX3 silencing in MCF-7 cells attenuated, while ectopic expression of ZFHX3 in T-47D cells enhanced, E 2 -mediated MYC expression, as detected by western blotting ( f , h ) and quantified by relative MYC ratio ( g , i ). Cells were treated with E 2 for 24 h at the indicated concentrations. siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . ** p < 0.01. ( a , b ) IHC staining was done in xenograft tumors. ( c – i ) cells were cultured in 2D plastic surface and examined with western blotting. Ratios of protein band intensities to those of their loading controls, with the control sample’s normalized to 1, are shown under western blot bands ( c – f , h ). Uncropped western blot images are available in .
    Figure Legend Snippet: ZFHX3 induces the expression of MYC and TBX3 in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) Detection of MYC, TBX3, OCT4, and SOX2 by IHC staining in xenograft tumors of T-47D cells ectopically expressing ZFHX3, as shown by representative IHC images (left) and quantification of cells with positive staining (right). Scale bar, 50 µm. ( c , d ) Ectopic expression of ZFHX3 in T-47D cells increased ( c ), while ZFHX3 silencing in MCF-7 cells decreased ( d ), the expression of MYC, TBX3, OCT4, NANOG, and SOX2, as detected by western blotting analysis. ( e ) Estrogen (E 2 ) increased MYC and TBX3 in MCF-7 cells, as detected by western blotting. ( f – i ) ZFHX3 silencing in MCF-7 cells attenuated, while ectopic expression of ZFHX3 in T-47D cells enhanced, E 2 -mediated MYC expression, as detected by western blotting ( f , h ) and quantified by relative MYC ratio ( g , i ). Cells were treated with E 2 for 24 h at the indicated concentrations. siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . ** p < 0.01. ( a , b ) IHC staining was done in xenograft tumors. ( c – i ) cells were cultured in 2D plastic surface and examined with western blotting. Ratios of protein band intensities to those of their loading controls, with the control sample’s normalized to 1, are shown under western blot bands ( c – f , h ). Uncropped western blot images are available in .

    Techniques Used: Expressing, Immunohistochemistry, Staining, Western Blot, Cell Culture

    ZFHX3 activates MYC and TBX3 transcription by binding to their promoters in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) ZFHX3 silencing in MCF-7 cells decreased, while ectopic expression of ZFHX3 in T-47D ( b ) cells increased, mRNA levels of MYC and TBX3 , as detected by real-time PCR. ( c ) Schematic for the construction of different MYC promoter-luciferase reporter plasmids. MYC-Luc, 2 kb promoter sequences; MYC-B-Luc, 1 kb; ΔMYC-Luc, the 171 bp from −1607 to −1437 bp upstream to the transcription initiation site was deleted. ( d , e ) ZFHX3 silencing in MCF-7 cells ( d ) and ZFHX3 expression in T-47D cells ( e ) had different effects on the activities of different MYC promoter constructs, as detected by luciferase reporter assay. ( f , g ) Location of PCR primer pairs, indicated by arrows, that define fragments A, B, C, and D in the MYC promoter ( f ) and detection of ZFHX3 binding to the C region (−1607 to −1437 bp) by ChIP-PCR. ( h ) Schematic for the construction of TBX3 promoter-reporter plasmids. L-TBX3-Luc and S-TBX3-Luc contain 1.52 kb and 0.51 kb DNA sequence, respectively, upstream to the TBX3 transcription initiation site. ( i , j ) Effects of ZFHX3 silencing in MCF-7 cells ( i ) and ZFHX3 expression in T-47D cells ( j ) on the two TBX3 constructs’ luciferase activities, as detected by luciferase reporter assay. ( k , l ) Location of PCR primer pairs, indicated by arrows, that define fragments a, b, and c in the TBX3 promoter ( k ) and detection of ZFHX3 binding to the b region (−1480 to −1292 bp) by ChIP-PCR ( l ). siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . ns, not significant; * p < 0.05; ** p < 0.01. Cells were cultured on a 2D plastic surface, then examined in real-time PCR, promoter-luciferase assay, and ChIP assay.
    Figure Legend Snippet: ZFHX3 activates MYC and TBX3 transcription by binding to their promoters in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) ZFHX3 silencing in MCF-7 cells decreased, while ectopic expression of ZFHX3 in T-47D ( b ) cells increased, mRNA levels of MYC and TBX3 , as detected by real-time PCR. ( c ) Schematic for the construction of different MYC promoter-luciferase reporter plasmids. MYC-Luc, 2 kb promoter sequences; MYC-B-Luc, 1 kb; ΔMYC-Luc, the 171 bp from −1607 to −1437 bp upstream to the transcription initiation site was deleted. ( d , e ) ZFHX3 silencing in MCF-7 cells ( d ) and ZFHX3 expression in T-47D cells ( e ) had different effects on the activities of different MYC promoter constructs, as detected by luciferase reporter assay. ( f , g ) Location of PCR primer pairs, indicated by arrows, that define fragments A, B, C, and D in the MYC promoter ( f ) and detection of ZFHX3 binding to the C region (−1607 to −1437 bp) by ChIP-PCR. ( h ) Schematic for the construction of TBX3 promoter-reporter plasmids. L-TBX3-Luc and S-TBX3-Luc contain 1.52 kb and 0.51 kb DNA sequence, respectively, upstream to the TBX3 transcription initiation site. ( i , j ) Effects of ZFHX3 silencing in MCF-7 cells ( i ) and ZFHX3 expression in T-47D cells ( j ) on the two TBX3 constructs’ luciferase activities, as detected by luciferase reporter assay. ( k , l ) Location of PCR primer pairs, indicated by arrows, that define fragments a, b, and c in the TBX3 promoter ( k ) and detection of ZFHX3 binding to the b region (−1480 to −1292 bp) by ChIP-PCR ( l ). siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . ns, not significant; * p < 0.05; ** p < 0.01. Cells were cultured on a 2D plastic surface, then examined in real-time PCR, promoter-luciferase assay, and ChIP assay.

    Techniques Used: Binding Assay, Expressing, Real-time Polymerase Chain Reaction, Luciferase, Construct, Reporter Assay, Sequencing, Cell Culture

    MYC and TBX3 play a role in ZFHX3-mediated sphere formation in Matrigel and ALDH + cell population in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) In T-47D cells ectopically expressing ZFHX3, MYC silencing, as confirmed by western blotting in 2D culture ( a ), and culture in Matrigel (3D culture) that attenuated sphere formation in both ZFHX3 and vector control groups ( b ). ( c , d ) In MCF-7 cells with ZFHX3 silencing, MYC ectopic expression, as confirmed by western blotting in 2D culture ( c ), and culture in Matrigel (3D culture) that did not significantly increase sphere number ( d ). ( e , f ) In T-47D cells ectopically expressing ZFHX3, TBX3 silencing, as confirmed by western blotting in 2D culture ( e ), and culture in Matrigel (3D culture) that attenuated sphere formation ( f ). ( g , h ) In MCF-7 cells with ZFHX3 silencing, TBX3 ectopic expression, as confirmed by western blotting in 2D culture ( g ), and culture in Matrigel (3D culture) that did not significantly increase sphere number ( h ). ( i , j ) ALDH + cells were then detected by flow cytometry in MCF-7 cells with ZFHX3 silencing and MYC or TBX3 overexpression ( i ) and T-47D cells with ZFHX3 overexpression and MYC or TBX3 knockdown ( j ). ns, not significant; * p < 0.05; ** p < 0.01. Cells were cultured on a 2D plastic surface. siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . Ratios of protein band intensities to those of their loading controls, with the control sample’s normalized to 1, are shown under western blot bands ( a , c , e , g ). Uncropped western blot images are available in .
    Figure Legend Snippet: MYC and TBX3 play a role in ZFHX3-mediated sphere formation in Matrigel and ALDH + cell population in ER + MCF-7 and T-47D breast cancer cells. ( a , b ) In T-47D cells ectopically expressing ZFHX3, MYC silencing, as confirmed by western blotting in 2D culture ( a ), and culture in Matrigel (3D culture) that attenuated sphere formation in both ZFHX3 and vector control groups ( b ). ( c , d ) In MCF-7 cells with ZFHX3 silencing, MYC ectopic expression, as confirmed by western blotting in 2D culture ( c ), and culture in Matrigel (3D culture) that did not significantly increase sphere number ( d ). ( e , f ) In T-47D cells ectopically expressing ZFHX3, TBX3 silencing, as confirmed by western blotting in 2D culture ( e ), and culture in Matrigel (3D culture) that attenuated sphere formation ( f ). ( g , h ) In MCF-7 cells with ZFHX3 silencing, TBX3 ectopic expression, as confirmed by western blotting in 2D culture ( g ), and culture in Matrigel (3D culture) that did not significantly increase sphere number ( h ). ( i , j ) ALDH + cells were then detected by flow cytometry in MCF-7 cells with ZFHX3 silencing and MYC or TBX3 overexpression ( i ) and T-47D cells with ZFHX3 overexpression and MYC or TBX3 knockdown ( j ). ns, not significant; * p < 0.05; ** p < 0.01. Cells were cultured on a 2D plastic surface. siCon, control siRNA; siZFHX3, siRNA against ZFHX3 . Ratios of protein band intensities to those of their loading controls, with the control sample’s normalized to 1, are shown under western blot bands ( a , c , e , g ). Uncropped western blot images are available in .

    Techniques Used: Expressing, Western Blot, Plasmid Preparation, Flow Cytometry, Over Expression, Cell Culture

    tbx3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc tbx3
    Tbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti tbx3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti tbx3
    <t>TBX3</t> is expressed in normal skeletal muscle and not over expressed in RMS cells. A. TBX3 is expressed in C2C12 cells throughout differentiation. C2C12 cells were differentiated for the indicted number of days and analyzed by western blot analysis. B. TBX3 is not over expressed in RMS cells. Western blot was probed with specific antibodies against TBX3 and GAPDH. Asterisk indicates the predicted size of TBX3. C. TBX3 is expressed in both normal tissue and primary RMS tumors. Immunofluorescence staining of primary RMS tumor and normal skeletal muscle sections using TBX3 specific antibodies is shown. Images were taken at 100X magnification and scale bars represent 20 μm.
    Anti Tbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tbx3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    1) Product Images from "TBX2 represses PTEN in rhabdomyosarcoma and skeletal muscle"

    Article Title: TBX2 represses PTEN in rhabdomyosarcoma and skeletal muscle

    Journal: Oncogene

    doi: 10.1038/onc.2015.486

    TBX3 is expressed in normal skeletal muscle and not over expressed in RMS cells. A. TBX3 is expressed in C2C12 cells throughout differentiation. C2C12 cells were differentiated for the indicted number of days and analyzed by western blot analysis. B. TBX3 is not over expressed in RMS cells. Western blot was probed with specific antibodies against TBX3 and GAPDH. Asterisk indicates the predicted size of TBX3. C. TBX3 is expressed in both normal tissue and primary RMS tumors. Immunofluorescence staining of primary RMS tumor and normal skeletal muscle sections using TBX3 specific antibodies is shown. Images were taken at 100X magnification and scale bars represent 20 μm.
    Figure Legend Snippet: TBX3 is expressed in normal skeletal muscle and not over expressed in RMS cells. A. TBX3 is expressed in C2C12 cells throughout differentiation. C2C12 cells were differentiated for the indicted number of days and analyzed by western blot analysis. B. TBX3 is not over expressed in RMS cells. Western blot was probed with specific antibodies against TBX3 and GAPDH. Asterisk indicates the predicted size of TBX3. C. TBX3 is expressed in both normal tissue and primary RMS tumors. Immunofluorescence staining of primary RMS tumor and normal skeletal muscle sections using TBX3 specific antibodies is shown. Images were taken at 100X magnification and scale bars represent 20 μm.

    Techniques Used: Western Blot, Immunofluorescence, Staining

    TBX3 does not repress PTEN in C2C12 cells. A-B. Stable C2C12 cell lines over expressing V5 tagged TBX3 (TBX3) or a vector control (pEF) were assayed for expression of TBX3 by qRT-PCR (A.) and western blot analysis with indicated antibodies (B.). C-D. Over expression of TBX3 does not repress PTEN. The expression of PTEN was assayed in the cell lines described in A. by qRT-PCR (C.) and western blot analysis (D.) E. Over expression of TBX3 does not inhibit myogenic differentiation. Cell lines described in A. were differentiated for 2 days and gene expression for the indicated genes was analyzed by qRT-PCR. F. TBX2, not TBX3, inhibits myogenesis. C2C12 cells were transiently transfected with expression constructs for vector control, TBX2 or TBX3 and differentiated for 24 hours prior to RNA isolation 48 hours post transfection. Gene expression for the indicated genes was analyzed by qRT-PCR. G. TBX3 does not regulate PTEN in C2C12 cells. Cells described in F. were analyzed for PTEN expression by qRT-PCR.
    Figure Legend Snippet: TBX3 does not repress PTEN in C2C12 cells. A-B. Stable C2C12 cell lines over expressing V5 tagged TBX3 (TBX3) or a vector control (pEF) were assayed for expression of TBX3 by qRT-PCR (A.) and western blot analysis with indicated antibodies (B.). C-D. Over expression of TBX3 does not repress PTEN. The expression of PTEN was assayed in the cell lines described in A. by qRT-PCR (C.) and western blot analysis (D.) E. Over expression of TBX3 does not inhibit myogenic differentiation. Cell lines described in A. were differentiated for 2 days and gene expression for the indicated genes was analyzed by qRT-PCR. F. TBX2, not TBX3, inhibits myogenesis. C2C12 cells were transiently transfected with expression constructs for vector control, TBX2 or TBX3 and differentiated for 24 hours prior to RNA isolation 48 hours post transfection. Gene expression for the indicated genes was analyzed by qRT-PCR. G. TBX3 does not regulate PTEN in C2C12 cells. Cells described in F. were analyzed for PTEN expression by qRT-PCR.

    Techniques Used: Expressing, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Over Expression, Transfection, Construct, Isolation

    anti tbx3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti tbx3
    Anti Tbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc tbx3
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    Cell Signaling Technology Inc anti tbx3
    <t>TBX3</t> is expressed in normal skeletal muscle and not over expressed in RMS cells. A. TBX3 is expressed in C2C12 cells throughout differentiation. C2C12 cells were differentiated for the indicted number of days and analyzed by western blot analysis. B. TBX3 is not over expressed in RMS cells. Western blot was probed with specific antibodies against TBX3 and GAPDH. Asterisk indicates the predicted size of TBX3. C. TBX3 is expressed in both normal tissue and primary RMS tumors. Immunofluorescence staining of primary RMS tumor and normal skeletal muscle sections using TBX3 specific antibodies is shown. Images were taken at 100X magnification and scale bars represent 20 μm.
    Anti Tbx3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tbx3/product/Cell Signaling Technology Inc
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    TBX3 is expressed in normal skeletal muscle and not over expressed in RMS cells. A. TBX3 is expressed in C2C12 cells throughout differentiation. C2C12 cells were differentiated for the indicted number of days and analyzed by western blot analysis. B. TBX3 is not over expressed in RMS cells. Western blot was probed with specific antibodies against TBX3 and GAPDH. Asterisk indicates the predicted size of TBX3. C. TBX3 is expressed in both normal tissue and primary RMS tumors. Immunofluorescence staining of primary RMS tumor and normal skeletal muscle sections using TBX3 specific antibodies is shown. Images were taken at 100X magnification and scale bars represent 20 μm.

    Journal: Oncogene

    Article Title: TBX2 represses PTEN in rhabdomyosarcoma and skeletal muscle

    doi: 10.1038/onc.2015.486

    Figure Lengend Snippet: TBX3 is expressed in normal skeletal muscle and not over expressed in RMS cells. A. TBX3 is expressed in C2C12 cells throughout differentiation. C2C12 cells were differentiated for the indicted number of days and analyzed by western blot analysis. B. TBX3 is not over expressed in RMS cells. Western blot was probed with specific antibodies against TBX3 and GAPDH. Asterisk indicates the predicted size of TBX3. C. TBX3 is expressed in both normal tissue and primary RMS tumors. Immunofluorescence staining of primary RMS tumor and normal skeletal muscle sections using TBX3 specific antibodies is shown. Images were taken at 100X magnification and scale bars represent 20 μm.

    Article Snippet: The antibodies used include anti-PTEN (559600, BD Pharmingen), anti-phospho PTEN (9554, Cell Signaling), anti-TBX2 (C-17, Santa Cruz Biotechnology), anti-TBX2 (gift of C. Goding, University of Oxford), anti-TBX3 (A-20, Santa Cruz Biotechnology), anti-AKT (pan, C67E7, Cell Signaling), anti-phospho-AKT (Ser 473 D9E, Cell Signaling) and anti-GAPDH (6C5, Millipore).

    Techniques: Western Blot, Immunofluorescence, Staining

    TBX3 does not repress PTEN in C2C12 cells. A-B. Stable C2C12 cell lines over expressing V5 tagged TBX3 (TBX3) or a vector control (pEF) were assayed for expression of TBX3 by qRT-PCR (A.) and western blot analysis with indicated antibodies (B.). C-D. Over expression of TBX3 does not repress PTEN. The expression of PTEN was assayed in the cell lines described in A. by qRT-PCR (C.) and western blot analysis (D.) E. Over expression of TBX3 does not inhibit myogenic differentiation. Cell lines described in A. were differentiated for 2 days and gene expression for the indicated genes was analyzed by qRT-PCR. F. TBX2, not TBX3, inhibits myogenesis. C2C12 cells were transiently transfected with expression constructs for vector control, TBX2 or TBX3 and differentiated for 24 hours prior to RNA isolation 48 hours post transfection. Gene expression for the indicated genes was analyzed by qRT-PCR. G. TBX3 does not regulate PTEN in C2C12 cells. Cells described in F. were analyzed for PTEN expression by qRT-PCR.

    Journal: Oncogene

    Article Title: TBX2 represses PTEN in rhabdomyosarcoma and skeletal muscle

    doi: 10.1038/onc.2015.486

    Figure Lengend Snippet: TBX3 does not repress PTEN in C2C12 cells. A-B. Stable C2C12 cell lines over expressing V5 tagged TBX3 (TBX3) or a vector control (pEF) were assayed for expression of TBX3 by qRT-PCR (A.) and western blot analysis with indicated antibodies (B.). C-D. Over expression of TBX3 does not repress PTEN. The expression of PTEN was assayed in the cell lines described in A. by qRT-PCR (C.) and western blot analysis (D.) E. Over expression of TBX3 does not inhibit myogenic differentiation. Cell lines described in A. were differentiated for 2 days and gene expression for the indicated genes was analyzed by qRT-PCR. F. TBX2, not TBX3, inhibits myogenesis. C2C12 cells were transiently transfected with expression constructs for vector control, TBX2 or TBX3 and differentiated for 24 hours prior to RNA isolation 48 hours post transfection. Gene expression for the indicated genes was analyzed by qRT-PCR. G. TBX3 does not regulate PTEN in C2C12 cells. Cells described in F. were analyzed for PTEN expression by qRT-PCR.

    Article Snippet: The antibodies used include anti-PTEN (559600, BD Pharmingen), anti-phospho PTEN (9554, Cell Signaling), anti-TBX2 (C-17, Santa Cruz Biotechnology), anti-TBX2 (gift of C. Goding, University of Oxford), anti-TBX3 (A-20, Santa Cruz Biotechnology), anti-AKT (pan, C67E7, Cell Signaling), anti-phospho-AKT (Ser 473 D9E, Cell Signaling) and anti-GAPDH (6C5, Millipore).

    Techniques: Expressing, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Over Expression, Transfection, Construct, Isolation