rabbit polyclonal anti k2p 3 1  (Alomone Labs)


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    Alomone Labs rabbit polyclonal anti k2p 3 1
    Rabbit Polyclonal Anti K2p 3 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti k2p 3 1/product/Alomone Labs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti k2p 3 1 - by Bioz Stars, 2022-09
    94/100 stars

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    Alomone Labs task 1
    TREK ‐1 deficiency does not affect activity or expression of other channels important for  SAN  function. A and B, Representative current traces of pacemaker current ( I f ) and summary current‐voltage data in  WT  and  Kcnk2 −/− SAN  cells (n=5 from 4 preparations for  WT , n=5 from 3 preparations for  Kcnk2 −/− ,  P = NS ). Voltage ramp protocol is shown in inset. C, Immunoblots showing expression of voltage‐gated K +  channels important for  SAN  cell repolarization (K v 2.1, K v 4.2, K v 4.3),  HCN 4, and K 2P  family member  TASK ‐1 in detergent‐soluble lysates from  WT  and α MHC ‐Kcnk2 f/f  ( Kcnk2 −/− )  SAN  (3 pooled samples from 3 preparations). D, Isolated  WT  and  Kcnk2 −/−  adult  SAN  myocytes immunostained for  HCN 4,  TREK ‐1, and  TASK ‐1. Scale bar=5 μm. SAN indicates sinoatrial node; WT, wild type.
    Task 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/task 1/product/Alomone Labs
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    task 1 - by Bioz Stars, 2022-09
    94/100 stars
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    TREK ‐1 deficiency does not affect activity or expression of other channels important for  SAN  function. A and B, Representative current traces of pacemaker current ( I f ) and summary current‐voltage data in  WT  and  Kcnk2 −/− SAN  cells (n=5 from 4 preparations for  WT , n=5 from 3 preparations for  Kcnk2 −/− ,  P = NS ). Voltage ramp protocol is shown in inset. C, Immunoblots showing expression of voltage‐gated K +  channels important for  SAN  cell repolarization (K v 2.1, K v 4.2, K v 4.3),  HCN 4, and K 2P  family member  TASK ‐1 in detergent‐soluble lysates from  WT  and α MHC ‐Kcnk2 f/f  ( Kcnk2 −/− )  SAN  (3 pooled samples from 3 preparations). D, Isolated  WT  and  Kcnk2 −/−  adult  SAN  myocytes immunostained for  HCN 4,  TREK ‐1, and  TASK ‐1. Scale bar=5 μm. SAN indicates sinoatrial node; WT, wild type.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Two‐Pore K+ Channel TREK‐1 Regulates Sinoatrial Node Membrane Excitability

    doi: 10.1161/JAHA.115.002865

    Figure Lengend Snippet: TREK ‐1 deficiency does not affect activity or expression of other channels important for SAN function. A and B, Representative current traces of pacemaker current ( I f ) and summary current‐voltage data in WT and Kcnk2 −/− SAN cells (n=5 from 4 preparations for WT , n=5 from 3 preparations for Kcnk2 −/− , P = NS ). Voltage ramp protocol is shown in inset. C, Immunoblots showing expression of voltage‐gated K + channels important for SAN cell repolarization (K v 2.1, K v 4.2, K v 4.3), HCN 4, and K 2P family member TASK ‐1 in detergent‐soluble lysates from WT and α MHC ‐Kcnk2 f/f ( Kcnk2 −/− ) SAN (3 pooled samples from 3 preparations). D, Isolated WT and Kcnk2 −/− adult SAN myocytes immunostained for HCN 4, TREK ‐1, and TASK ‐1. Scale bar=5 μm. SAN indicates sinoatrial node; WT, wild type.

    Article Snippet: The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: TREK‐1 (C‐terminal: sc50412, N‐terminal: sc11554; Santa Cruz Biotechnology), βIV ‐spectrin (N‐terminal, HPA043370, lot A96519; Sigma‐Aldrich), Kir 2.1 (Alomone), Kv 2.1 (NeuroMab), Kv 4.2 (Alomone), Kv4.3, TASK‐1 (APC024; Alomone), HCN4 (32675, lot GR153269‐7; Abcam), N‐cadherin (333900, lot 1397763A; Invitrogen), Connexin43 (MAB3067, lot 23070602; Invitrogen), and GAPDH (10R‐G109A; Fitzgerald).

    Techniques: Activity Assay, Expressing, Western Blot, Isolation

    Stimulatory effect of leptin on pSTAT3/STAT3 and TASK-1,-2,-3 channel protein expression in CBs. Gel images and bar charts showing quantitative analysis of leptin-stimulated expression of ob-Rb (A) pSTAT3/STAT3 (B) and TASK-1,-2,-3 (C–E) in the CB ( n = 8) of each group ( n = 4). ** P

    Journal: Frontiers in Physiology

    Article Title: Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression

    doi: 10.3389/fphys.2018.00249

    Figure Lengend Snippet: Stimulatory effect of leptin on pSTAT3/STAT3 and TASK-1,-2,-3 channel protein expression in CBs. Gel images and bar charts showing quantitative analysis of leptin-stimulated expression of ob-Rb (A) pSTAT3/STAT3 (B) and TASK-1,-2,-3 (C–E) in the CB ( n = 8) of each group ( n = 4). ** P

    Article Snippet: The membranes were blocked with bovine serum albumin and incubated at 4°C overnight with primary antibodies anti-ob-R (1:2000, #ab5593, Abcam, USA), anti-TASK-1 (1:200, #APC024, Alomone labs, Israel), anti-TASK-2 (1:200, #APC037, Alomone labs, Israel), anti-TASK-3 (1:200, #APC044, Alomone labs, Israel), anti-pSTAT3 (1:2000, #9145, Cell Signaling Technology, USA), anti-STAT3 (1:1000, #9139, Cell Signaling Technology, USA), and anti-β-actin (1:3,000, #T0022, Affinity Biosciences, USA).

    Techniques: Expressing

    Downregulation of pSTAT3/STAT3 and TASK-1,-2,-3 channels in CBs. Gel images and bar charts showing quantitative analysis of expression of ob-Rb (A,B) , pSTAT3/STAT3 (C,D) , and TASK-1,-2,-3 (E–H) in the CB ( n = 8) from each group ( n = 4). * P

    Journal: Frontiers in Physiology

    Article Title: Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression

    doi: 10.3389/fphys.2018.00249

    Figure Lengend Snippet: Downregulation of pSTAT3/STAT3 and TASK-1,-2,-3 channels in CBs. Gel images and bar charts showing quantitative analysis of expression of ob-Rb (A,B) , pSTAT3/STAT3 (C,D) , and TASK-1,-2,-3 (E–H) in the CB ( n = 8) from each group ( n = 4). * P

    Article Snippet: The membranes were blocked with bovine serum albumin and incubated at 4°C overnight with primary antibodies anti-ob-R (1:2000, #ab5593, Abcam, USA), anti-TASK-1 (1:200, #APC024, Alomone labs, Israel), anti-TASK-2 (1:200, #APC037, Alomone labs, Israel), anti-TASK-3 (1:200, #APC044, Alomone labs, Israel), anti-pSTAT3 (1:2000, #9145, Cell Signaling Technology, USA), anti-STAT3 (1:1000, #9139, Cell Signaling Technology, USA), and anti-β-actin (1:3,000, #T0022, Affinity Biosciences, USA).

    Techniques: Expressing

    Stimulatory effect of leptin on pSTAT3/STAT3 and TASK-1,-2,-3 channel protein expression in CBs. Gel images and bar charts showing quantitative analysis of leptin-stimulated expression of ob-Rb (A) pSTAT3/STAT3 (B) and TASK-1,-2,-3 (C–E) in the CB ( n = 8) of each group ( n = 4). ** P

    Journal: Frontiers in Physiology

    Article Title: Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression

    doi: 10.3389/fphys.2018.00249

    Figure Lengend Snippet: Stimulatory effect of leptin on pSTAT3/STAT3 and TASK-1,-2,-3 channel protein expression in CBs. Gel images and bar charts showing quantitative analysis of leptin-stimulated expression of ob-Rb (A) pSTAT3/STAT3 (B) and TASK-1,-2,-3 (C–E) in the CB ( n = 8) of each group ( n = 4). ** P

    Article Snippet: The membranes were blocked with bovine serum albumin and incubated at 4°C overnight with primary antibodies anti-ob-R (1:2000, #ab5593, Abcam, USA), anti-TASK-1 (1:200, #APC024, Alomone labs, Israel), anti-TASK-2 (1:200, #APC037, Alomone labs, Israel), anti-TASK-3 (1:200, #APC044, Alomone labs, Israel), anti-pSTAT3 (1:2000, #9145, Cell Signaling Technology, USA), anti-STAT3 (1:1000, #9139, Cell Signaling Technology, USA), and anti-β-actin (1:3,000, #T0022, Affinity Biosciences, USA).

    Techniques: Expressing

    Immunocytochemistry showing subcellular localization of five (TWIK1, TREK1, TREK2, TASK1, and TASK3) two-pore potassium (K2P) channel subtypes and either P2Y 2 or P2Y 4 receptors at or near the apical membrane. P2Y 2 -R or P2Y 4 -R (red); K2P subtypes (green);

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: P2Y receptor regulation of K2P channels that facilitate K+ secretion by human mammary epithelial cells

    doi: 10.1152/ajpcell.00342.2016

    Figure Lengend Snippet: Immunocytochemistry showing subcellular localization of five (TWIK1, TREK1, TREK2, TASK1, and TASK3) two-pore potassium (K2P) channel subtypes and either P2Y 2 or P2Y 4 receptors at or near the apical membrane. P2Y 2 -R or P2Y 4 -R (red); K2P subtypes (green);

    Article Snippet: The primary antibodies used were goat anti-TWIK1 (1:200) (Santa Cruz), rabbit anti-TREK1 (1:200), rabbit anti-TREK2 (1:200), rabbit anti-TASK1 (1:200) (Alomone Laboratories, Jerusalem, Israel), and rabbit anti-TASK3 (1:200) (Abcam, Cambridge, UK).

    Techniques: Immunocytochemistry