Structured Review

Abcam anti tapa1 antibody epr4244
miR-1258 knockdown attenuates the influence of exosomal circ_0000519 downregulation on NSCLC cell growth and metastasis. H2170 and A549 cells were transfected with <t>anti-NC</t> or <t>anti-miR-1258,</t> and then, the transfected or non-transfected cells were incubated with the exosomes from the H1299 cells transfected with si-NC or si-circ_0000519. (a) miR-1258 expression was detected by qRT-PCR in the transfected cells. (b and c) Cell growth was evaluated via EdU staining and colony formation assays in the treated cells. (d and e) Cell migration and invasion were examined via transwell analysis in the treated cells. (f and g) Cyclin D1, Vimentin and MMP9 abundances were examined via Western blotting in the treated cells. *** P
Anti Tapa1 Antibody Epr4244, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 40 article reviews
Price from $9.99 to $1999.99
anti tapa1 antibody epr4244 - by Bioz Stars, 2022-10
95/100 stars

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1) Product Images from "Exosomal hsa_circ_0000519 modulates the NSCLC cell growth and metastasis via miR-1258/RHOV axis"

Article Title: Exosomal hsa_circ_0000519 modulates the NSCLC cell growth and metastasis via miR-1258/RHOV axis

Journal: Open Medicine

doi: 10.1515/med-2022-0428

miR-1258 knockdown attenuates the influence of exosomal circ_0000519 downregulation on NSCLC cell growth and metastasis. H2170 and A549 cells were transfected with anti-NC or anti-miR-1258, and then, the transfected or non-transfected cells were incubated with the exosomes from the H1299 cells transfected with si-NC or si-circ_0000519. (a) miR-1258 expression was detected by qRT-PCR in the transfected cells. (b and c) Cell growth was evaluated via EdU staining and colony formation assays in the treated cells. (d and e) Cell migration and invasion were examined via transwell analysis in the treated cells. (f and g) Cyclin D1, Vimentin and MMP9 abundances were examined via Western blotting in the treated cells. *** P
Figure Legend Snippet: miR-1258 knockdown attenuates the influence of exosomal circ_0000519 downregulation on NSCLC cell growth and metastasis. H2170 and A549 cells were transfected with anti-NC or anti-miR-1258, and then, the transfected or non-transfected cells were incubated with the exosomes from the H1299 cells transfected with si-NC or si-circ_0000519. (a) miR-1258 expression was detected by qRT-PCR in the transfected cells. (b and c) Cell growth was evaluated via EdU staining and colony formation assays in the treated cells. (d and e) Cell migration and invasion were examined via transwell analysis in the treated cells. (f and g) Cyclin D1, Vimentin and MMP9 abundances were examined via Western blotting in the treated cells. *** P

Techniques Used: Transfection, Incubation, Expressing, Quantitative RT-PCR, Staining, Migration, Western Blot

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  • cd81  (Abcam)
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    Abcam cd81
    Levels of serum exosomal miR-21 are over-expressed in smokers and COPD patients and are negatively related to lung function . Exosomes from sera of non-smokers (n=26), smokers (n=24), and smokers with COPD (n=29) were isolated by ExoQuick. (A) Western blots of CD9, CD63, and <t>CD81</t> in serum exosomes. (B) Representative electron micrograph of serum exosomes (bars = 200 nm). (C) The levels of serum exosomal miR-21 were determined by quantitative RT-PCR. Data are means ± SD. * P
    Cd81, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd81/product/Abcam
    Average 96 stars, based on 1 article reviews
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    cd81 - by Bioz Stars, 2022-10
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    Levels of serum exosomal miR-21 are over-expressed in smokers and COPD patients and are negatively related to lung function . Exosomes from sera of non-smokers (n=26), smokers (n=24), and smokers with COPD (n=29) were isolated by ExoQuick. (A) Western blots of CD9, CD63, and CD81 in serum exosomes. (B) Representative electron micrograph of serum exosomes (bars = 200 nm). (C) The levels of serum exosomal miR-21 were determined by quantitative RT-PCR. Data are means ± SD. * P

    Journal: Theranostics

    Article Title: Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking

    doi: 10.7150/thno.27876

    Figure Lengend Snippet: Levels of serum exosomal miR-21 are over-expressed in smokers and COPD patients and are negatively related to lung function . Exosomes from sera of non-smokers (n=26), smokers (n=24), and smokers with COPD (n=29) were isolated by ExoQuick. (A) Western blots of CD9, CD63, and CD81 in serum exosomes. (B) Representative electron micrograph of serum exosomes (bars = 200 nm). (C) The levels of serum exosomal miR-21 were determined by quantitative RT-PCR. Data are means ± SD. * P

    Article Snippet: Membranes were then incubated overnight at 4ºC with a primary antibody for collagen I (1:2,000, ab138492, Abcam), α-SMA (1:2,000, ab7817, Abcam), hypoxia inducible factor-1 alpha (HIF-1α) (1:1,000, #36169, Cell Signaling Technology), von Hippel Lindau protein (pVHL) (1:1,000, sc-17780, Santa Cruz Biotechnology), tubulin (1:1,000, AF0001, Beyotime), CD9 (1:2,000, ab92726, Abcam), CD63 (1:1,000, ab68418, Abcam), CD81 (1:1,000, ab109201, Abcam), or heat shock protein 90k Da beta 1 (HSP90B1) (1:1,000, #2104, Cell Signaling Technology).

    Techniques: Isolation, Western Blot, Quantitative RT-PCR

    Exosomes derived from CSE-treated HBE cells transfer miR-21 to MRC-5 cells, which promotes the myofibroblast differentiation phenotype. HBE-Exo, exosomes derived from normal HBE cells; CHBE-Exo, exosomes derived from HBE cells treated with CSE. Densities of bands were quantified by Image J software. Tubulin levels, measured in parallel, served as controls. Exosomes from normal or CSE-treated HBE cells were fractionated by ExoQuick-TC. (A) Western blots of CD9, CD63, CD81, and HSP90B1 in exosomes. (B) Electron microscopic images of exosomes (bars = 100 nm). (C) Particle number and size analysis of HBE-exo or CHBE-exo were determined by dynamic light scattering using a ZetaView® nanoparticle tracker (ParticleMetrix, Germany). (D) Microscopic images of MRC-5 cells after incubation with PKH67-labeled (green) exosomes; nuclei were stained with DAPI (blue). (E) The levels of miR-21 in exosomes derived from normal or CSE-treated HBE cells were determined by qRT-PCR. MRC-5 cells were treated exosomes (50 μg/mL) derived from normal or CSE-treated HBE cells. (F) The levels of miR-21 in MRC-5 cells were determined by qRT-PCR. (G) Western blots were performed, and (H) relative protein levels of α-SMA and collagen I were determined for MRC-5 cells. Data are means ± SD, n=3 cultures. * P

    Journal: Theranostics

    Article Title: Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking

    doi: 10.7150/thno.27876

    Figure Lengend Snippet: Exosomes derived from CSE-treated HBE cells transfer miR-21 to MRC-5 cells, which promotes the myofibroblast differentiation phenotype. HBE-Exo, exosomes derived from normal HBE cells; CHBE-Exo, exosomes derived from HBE cells treated with CSE. Densities of bands were quantified by Image J software. Tubulin levels, measured in parallel, served as controls. Exosomes from normal or CSE-treated HBE cells were fractionated by ExoQuick-TC. (A) Western blots of CD9, CD63, CD81, and HSP90B1 in exosomes. (B) Electron microscopic images of exosomes (bars = 100 nm). (C) Particle number and size analysis of HBE-exo or CHBE-exo were determined by dynamic light scattering using a ZetaView® nanoparticle tracker (ParticleMetrix, Germany). (D) Microscopic images of MRC-5 cells after incubation with PKH67-labeled (green) exosomes; nuclei were stained with DAPI (blue). (E) The levels of miR-21 in exosomes derived from normal or CSE-treated HBE cells were determined by qRT-PCR. MRC-5 cells were treated exosomes (50 μg/mL) derived from normal or CSE-treated HBE cells. (F) The levels of miR-21 in MRC-5 cells were determined by qRT-PCR. (G) Western blots were performed, and (H) relative protein levels of α-SMA and collagen I were determined for MRC-5 cells. Data are means ± SD, n=3 cultures. * P

    Article Snippet: Membranes were then incubated overnight at 4ºC with a primary antibody for collagen I (1:2,000, ab138492, Abcam), α-SMA (1:2,000, ab7817, Abcam), hypoxia inducible factor-1 alpha (HIF-1α) (1:1,000, #36169, Cell Signaling Technology), von Hippel Lindau protein (pVHL) (1:1,000, sc-17780, Santa Cruz Biotechnology), tubulin (1:1,000, AF0001, Beyotime), CD9 (1:2,000, ab92726, Abcam), CD63 (1:1,000, ab68418, Abcam), CD81 (1:1,000, ab109201, Abcam), or heat shock protein 90k Da beta 1 (HSP90B1) (1:1,000, #2104, Cell Signaling Technology).

    Techniques: Derivative Assay, Software, Western Blot, Incubation, Labeling, Staining, Quantitative RT-PCR

    Comparison of extracellular vesicles (EVs) purified by ultracentrifugation and P/PEG precipitation. (A) Representative transmission electron microscopy of EVs isolated by ultracentrifugation (UC) or by P/PEG precipitation and negatively stained with NanoVan. EVs were viewed using a JEOL Jem 1010 electron microscope (black line, 100 nm). Three experiments were performed with similar results. (B) Representative western blot analysis of CD63, CD9, CD81 and Actin expression by EVs isolated with UC or by P/PEG precipitation from serum, saliva and human liver stem cell (HLSC) (four experiments were performed with similar results) and of apolipoprotein B100 (ApoB100) and apolipoprotein A1 (ApoA1) associated with EVs (five experiments were performed with similar results).

    Journal: International Journal of Molecular Medicine

    Article Title: Charge-based precipitation of extracellular vesicles

    doi: 10.3892/ijmm.2016.2759

    Figure Lengend Snippet: Comparison of extracellular vesicles (EVs) purified by ultracentrifugation and P/PEG precipitation. (A) Representative transmission electron microscopy of EVs isolated by ultracentrifugation (UC) or by P/PEG precipitation and negatively stained with NanoVan. EVs were viewed using a JEOL Jem 1010 electron microscope (black line, 100 nm). Three experiments were performed with similar results. (B) Representative western blot analysis of CD63, CD9, CD81 and Actin expression by EVs isolated with UC or by P/PEG precipitation from serum, saliva and human liver stem cell (HLSC) (four experiments were performed with similar results) and of apolipoprotein B100 (ApoB100) and apolipoprotein A1 (ApoA1) associated with EVs (five experiments were performed with similar results).

    Article Snippet: Protein samples were separated by 4–15% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subjected to immunoblotting with rabbit polyclonal antibodies (1:1000 dilutions) anti-CD9 (Cat. no. ab155825), CD63 (Cat. no. ab199921), CD81 (Cat. no. ab109201), anti-apolipoprotein B100 (ApoB100; 1:5,000 dilution; Cat. no. ab20737) and goat polyclonal antibody anti-apolipoprotein A1 (ApoA1; 1:5,000 dilution; Cat. no. ab7613) (Abcam, Cambridge, UK).

    Techniques: Purification, Transmission Assay, Electron Microscopy, Isolation, Staining, Microscopy, Western Blot, Expressing

    Exosomes derived from CSE-treated HBE cells transfer miR-21 to MRC-5 cells, which promotes the myofibroblast differentiation phenotype. HBE-Exo, exosomes derived from normal HBE cells; CHBE-Exo, exosomes derived from HBE cells treated with CSE. Densities of bands were quantified by Image J software. Tubulin levels, measured in parallel, served as controls. Exosomes from normal or CSE-treated HBE cells were fractionated by ExoQuick-TC. (A) Western blots of CD9, CD63, CD81, and HSP90B1 in exosomes. (B) Electron microscopic images of exosomes (bars = 100 nm). (C) Particle number and size analysis of HBE-exo or CHBE-exo were determined by dynamic light scattering using a ZetaView® nanoparticle tracker (ParticleMetrix, Germany). (D) Microscopic images of MRC-5 cells after incubation with PKH67-labeled (green) exosomes; nuclei were stained with DAPI (blue). (E) The levels of miR-21 in exosomes derived from normal or CSE-treated HBE cells were determined by qRT-PCR. MRC-5 cells were treated exosomes (50 μg/mL) derived from normal or CSE-treated HBE cells. (F) The levels of miR-21 in MRC-5 cells were determined by qRT-PCR. (G) Western blots were performed, and (H) relative protein levels of α-SMA and collagen I were determined for MRC-5 cells. Data are means ± SD, n=3 cultures. * P

    Journal: Theranostics

    Article Title: Exosomal microRNA-21 derived from bronchial epithelial cells is involved in aberrant epithelium-fibroblast cross-talk in COPD induced by cigarette smoking

    doi: 10.7150/thno.27876

    Figure Lengend Snippet: Exosomes derived from CSE-treated HBE cells transfer miR-21 to MRC-5 cells, which promotes the myofibroblast differentiation phenotype. HBE-Exo, exosomes derived from normal HBE cells; CHBE-Exo, exosomes derived from HBE cells treated with CSE. Densities of bands were quantified by Image J software. Tubulin levels, measured in parallel, served as controls. Exosomes from normal or CSE-treated HBE cells were fractionated by ExoQuick-TC. (A) Western blots of CD9, CD63, CD81, and HSP90B1 in exosomes. (B) Electron microscopic images of exosomes (bars = 100 nm). (C) Particle number and size analysis of HBE-exo or CHBE-exo were determined by dynamic light scattering using a ZetaView® nanoparticle tracker (ParticleMetrix, Germany). (D) Microscopic images of MRC-5 cells after incubation with PKH67-labeled (green) exosomes; nuclei were stained with DAPI (blue). (E) The levels of miR-21 in exosomes derived from normal or CSE-treated HBE cells were determined by qRT-PCR. MRC-5 cells were treated exosomes (50 μg/mL) derived from normal or CSE-treated HBE cells. (F) The levels of miR-21 in MRC-5 cells were determined by qRT-PCR. (G) Western blots were performed, and (H) relative protein levels of α-SMA and collagen I were determined for MRC-5 cells. Data are means ± SD, n=3 cultures. * P

    Article Snippet: Membranes were then incubated overnight at 4ºC with a primary antibody for collagen I (1:2,000, ab138492, Abcam), α-SMA (1:2,000, ab7817, Abcam), hypoxia inducible factor-1 alpha (HIF-1α) (1:1,000, #36169, Cell Signaling Technology), von Hippel Lindau protein (pVHL) (1:1,000, sc-17780, Santa Cruz Biotechnology), tubulin (1:1,000, AF0001, Beyotime), CD9 (1:2,000, ab92726, Abcam), CD63 (1:1,000, ab68418, Abcam), CD81 (1:1,000, ab109201, Abcam), or heat shock protein 90k Da beta 1 (HSP90B1) (1:1,000, #2104, Cell Signaling Technology).

    Techniques: Derivative Assay, Software, Western Blot, Incubation, Labeling, Staining, Quantitative RT-PCR

    Characterization of exosomes released from trastuzumab-resistant and -sensitive SKBR-3 cells. (A) Transmission electron microscopy images of the exosomes released by SKBR-3/Pr and SKBR-3/Tr cells. (B) Nanoparticle tracking analysis on an LM10 Nanosight unit demonstrating a mean size of 100 nm for SKBR-3/Tr and 120 nm for SKBR-3/Pr exosomes. The size distribution and relative concentration were calculated using the Nano-sight software. (C) Exosomal protein marker (CD63 and CD81) detection by western blotting from purified exosomes and cell extracts. (D) Flow cytometric analysis of the MFI for a panel of exosomal markers: CD9, CD63, CD81 and Alix. Data are presented as the median ± interquartile range of triplicate experiments. MFI, mean fluorescence intensity; CD, cluster of differentiation; Alix, programmed cell death 6-interacting protein.

    Journal: International Journal of Oncology

    Article Title: Exosome-mediated transfer of lncRNA-SNHG14 promotes trastuzumab chemoresistance in breast cancer

    doi: 10.3892/ijo.2018.4467

    Figure Lengend Snippet: Characterization of exosomes released from trastuzumab-resistant and -sensitive SKBR-3 cells. (A) Transmission electron microscopy images of the exosomes released by SKBR-3/Pr and SKBR-3/Tr cells. (B) Nanoparticle tracking analysis on an LM10 Nanosight unit demonstrating a mean size of 100 nm for SKBR-3/Tr and 120 nm for SKBR-3/Pr exosomes. The size distribution and relative concentration were calculated using the Nano-sight software. (C) Exosomal protein marker (CD63 and CD81) detection by western blotting from purified exosomes and cell extracts. (D) Flow cytometric analysis of the MFI for a panel of exosomal markers: CD9, CD63, CD81 and Alix. Data are presented as the median ± interquartile range of triplicate experiments. MFI, mean fluorescence intensity; CD, cluster of differentiation; Alix, programmed cell death 6-interacting protein.

    Article Snippet: The membranes were incubated overnight at 4°C with a 1:1,000 solution of primary rabbit antibodies: Anti-E-cadherin (Abcam, Cambridge, UK; cat. no. ab15148), anti-β-catenin (Abcam; cat. no. ab16051), anti-vimentin (Abcam; cat. no. ab92547), anti-cluster of differentiation (CD)63 (Abcam; cat. no. ab134045), anti-CD81 (Abcam; cat. no. ab109201), anti-cleaved poly(ADP-ribose) polymerase (PARP; cat. no. 5625; Cell Signaling Technology, Inc., Danvers, MA, USA), anti-cleaved caspase-3 (cat. no. 9664; Cell Signaling Technology, Inc.), anti-apoptosis regulator Bcl-2 (Bcl-2; cat. no. 4223, Cell Signaling Technology, Inc.), anti-apoptosis regulator BAX (Bax; cat. no. 5023; Cell Signaling Technology, Inc.), and anti-β-actin (Abcam; cat. no. ab8227).

    Techniques: Transmission Assay, Electron Microscopy, Concentration Assay, Software, Marker, Western Blot, Purification, Flow Cytometry, Fluorescence