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rabbit monoclonal sumo1  (Cell Signaling Technology Inc)
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Rabbit Monoclonal Sumo1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sumo1 Antibody, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by <t>SUMO1</t> and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.
Anti Sumo1, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by <t>SUMO1</t> and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.
Anti Sumo1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sumo1  (Danaher Inc)
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Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by <t>SUMO1</t> and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.
Sumo1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti sumo1  (Cell Signaling Technology Inc)
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Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by <t>SUMO1</t> and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.
Anti Sumo1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies against sumo1  (Affinity Biosciences)
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Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by <t>SUMO1</t> and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.
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Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by SUMO1 and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.

Journal: MedComm

Article Title: The SUMOylated RREB1 interacts with KDM1A to induce 5‐fluorouracil resistance via upregulating thymidylate synthase and activating DNA damage response pathway in colorectal cancer

doi: 10.1002/mco2.70105

Figure Lengend Snippet: Ras‐responsive element binding protein 1 (RREB1) was SUMOylated by SUMO1 and SUMO3 conjugation in colorectal cancer (CRC) cells. (A) SUMOylation of RREB1 was determined by western blot in HEK293T cells transfected with pFlag‐RREB1 plasmid combined with pHis‐SUMO1, pHis‐SUMO2, or pHis‐SUMO3. (B, C) RREB1 SUMOylation was detected in HCT116 cells. (D) RREB1 modified by endogenous SUMO1 or SUMO2/3 was detected in HCT116 cells stably expressing Flag‐RREB1. (E) RREB1 SUMOylation modified by His‐SUMO3 was increased by pHA‐UBC9 inducement in HCT116. (F, G) A SUMOylation inhibitor ML‐792 inhibited the His‐SUMO3‐modified RREB1 (F) or Myc‐SUMO1‐modified RREB1 (G) of HCT116 cells that were cotransfected with pFlag‐RREB1, pHA‐UBC9, and pHis‐SUMO3/pMyc‐SUMO1 plasmids. (H, I) RREB1 interacted with sentrin‐specific protease 1 (SENP1) in HCT116 cells. pMyc‐RREB1 and pFlag‐SENP1 were cotransfected into HCT116 cells as indicated for 48 h, and co‐immunoprecipitation (Co‐IP) was performed by anti‐Myc antibody (H) or anti‐Flag M2 antibody (I). (J) Flag‐SENP1 overexpression obviously reduced the RREB1 SUMOylation modified by His‐SUMO3 in HCT116 cells. (K) pFlag‐SENP1, pFlag‐RREB1, pHA‐UBC9, and pMyc‐SUMO1 were cotransfected in HCT116 cells for 48 h, then the SUMO1‐modified RREB1 was detected after IP. (L, M) Flag‐SENP1 C60S mutant abolished the catalytic analysis of wild‐type Flag‐SENP1 to inhibit the Myc‐SUMO1 (L) or His‐SUMO3 (M) conjugation with RREB1.

Article Snippet: Cell lysates and sodium dodecyl‐sulfate polyacrylamide gel electrophoresis (SDS‐) analysis was referred our previous methods., Specific primary antibodies were included rabbit anti‐RREB1 (A303‐129A, Bethyl), rabbit anti‐SUMO1 (ET1606‐53, HuaBio), rabbit anti‐SUMO2/3 (ET1701‐17, HuaBio), rabbit anti‐UBC9 (ET1610‐21, HuaBio), mouse anti‐Flag (F1804, Sigma‐Aldrich), His (HRP‐66005, Proteintech), rabbit anti‐TK1 (ET1702‐31, HuaBio), rabbit anti‐TS (ET1705‐24, HuaBio), rabbit anti‐KDM1A (R24798, zenbio), rabbit anti‐p‐Chk1 (HA721189, HuaBio), rabbit anti‐H2AX (AF6187, Affinity), and rabbit anti‐γH2AX (ET1602‐2, HuaBio).

Techniques: Binding Assay, Conjugation Assay, Western Blot, Transfection, Plasmid Preparation, Modification, Stable Transfection, Expressing, Immunoprecipitation, Co-Immunoprecipitation Assay, Over Expression, Mutagenesis

Ras‐responsive element binding protein 1 (RREB1) was mainly SUMOylated at K551, K885, and K913 sites. (A) Prediction of RREB1 SUMOylation using online software SUMOplot ( http://www.abgent.com/sumoplot ) and gps‐SUMO ( http://sumosp.biocuckoo.org/ ). (B, C) SUMOylation sites were determined by western blot in HEK293T cells. The SUMOylated Flag‐RREB1 or mutant was detected by anti‐SUMO1 (B) or anti‐SUMO2/3 (C) antibody. (D, E) The SUMOylation sites of RREB1 were measured at K551, K615, K885, K913 and their combination in HEK293T cells. Simultaneous mutation of K551 and K885 into arginine to generate Flag‐RREB1 2KR or mutations of K551, K885, and K913 into arginine to generate Flag‐RREB1 3KR . Similarly with panels B, C, pFlag‐RREB1 and mutants were cotransfected with pMyc‐SUMO1 or pHis‐SUMO3 into HEK293T cells for 48 h. (F, G) SUMO1 or SUMO3 modification on Flag‐RREB1 (F) or Flag‐RREB1 3KR (G) were detected in HEK‐293T cells.

Journal: MedComm

Article Title: The SUMOylated RREB1 interacts with KDM1A to induce 5‐fluorouracil resistance via upregulating thymidylate synthase and activating DNA damage response pathway in colorectal cancer

doi: 10.1002/mco2.70105

Figure Lengend Snippet: Ras‐responsive element binding protein 1 (RREB1) was mainly SUMOylated at K551, K885, and K913 sites. (A) Prediction of RREB1 SUMOylation using online software SUMOplot ( http://www.abgent.com/sumoplot ) and gps‐SUMO ( http://sumosp.biocuckoo.org/ ). (B, C) SUMOylation sites were determined by western blot in HEK293T cells. The SUMOylated Flag‐RREB1 or mutant was detected by anti‐SUMO1 (B) or anti‐SUMO2/3 (C) antibody. (D, E) The SUMOylation sites of RREB1 were measured at K551, K615, K885, K913 and their combination in HEK293T cells. Simultaneous mutation of K551 and K885 into arginine to generate Flag‐RREB1 2KR or mutations of K551, K885, and K913 into arginine to generate Flag‐RREB1 3KR . Similarly with panels B, C, pFlag‐RREB1 and mutants were cotransfected with pMyc‐SUMO1 or pHis‐SUMO3 into HEK293T cells for 48 h. (F, G) SUMO1 or SUMO3 modification on Flag‐RREB1 (F) or Flag‐RREB1 3KR (G) were detected in HEK‐293T cells.

Article Snippet: Cell lysates and sodium dodecyl‐sulfate polyacrylamide gel electrophoresis (SDS‐) analysis was referred our previous methods., Specific primary antibodies were included rabbit anti‐RREB1 (A303‐129A, Bethyl), rabbit anti‐SUMO1 (ET1606‐53, HuaBio), rabbit anti‐SUMO2/3 (ET1701‐17, HuaBio), rabbit anti‐UBC9 (ET1610‐21, HuaBio), mouse anti‐Flag (F1804, Sigma‐Aldrich), His (HRP‐66005, Proteintech), rabbit anti‐TK1 (ET1702‐31, HuaBio), rabbit anti‐TS (ET1705‐24, HuaBio), rabbit anti‐KDM1A (R24798, zenbio), rabbit anti‐p‐Chk1 (HA721189, HuaBio), rabbit anti‐H2AX (AF6187, Affinity), and rabbit anti‐γH2AX (ET1602‐2, HuaBio).

Techniques: Binding Assay, Software, Western Blot, Mutagenesis, Modification