Journal: International Journal of Molecular Sciences

Article Title: Store-Operated Ca 2+ Entry Contributes to Piezo1-Induced Ca 2+ Increase in Human Endometrial Stem Cells

doi: 10.3390/ijms23073763

Figure Lengend Snippet: ORAI1 , STIM1 and STIM2 expression in human eMSCs. ( A ) RT-PCR analysis of ORAI , STIM1 and STIM2 expression in eMSCs. M—size marker. Line 1—primers specific for ORAI1 , STIM1 and STIM2 amplified the PCR products of the expected sizes (78, 124 and 247 bp, correspondingly). Line 2—RT-PCR negative control in which reverse transcriptase was omitted. Shown are cropped gels with enhanced contrast. Original gel is shown in . The distribution of ORAI1 ( B ), STIM1 ( C ) and STIM2 ( D ). Immunoreactivity in eMSCs was examined with confocal microscopy. No staining of the cells was observed after incubation of the cells with only fluorescent secondary antibodies . Scale bar is 50 µm.

Article Snippet: Then cells were incubated with goat anti-rabbit polyclonal Abs against Piezo1 (Novus Bio, Centennial, CO, USA, cat. no. NBP1-78537) or rabbit polyclonal Abs against ORAI1 (Alomone Labs, Jerusalem, Israel, cat. no. ACC-060) or Abs against STIM1 (Alomone Labs, Jerusalem, Israel, cat. no. ACC-063) or STIM2 (Alomone Labs, Jerusalem, Israel, cat. no. ACC-064) at 4 °C overnight.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Amplification, Negative Control, Confocal Microscopy, Staining, Incubation

Journal: International Journal of Molecular Sciences

Article Title: High-Throughput Screen Detects Calcium Signaling Dysfunction in Hutchinson-Gilford Progeria Syndrome

doi: 10.3390/ijms22147327

Figure Lengend Snippet: Ca 2+ transporters localized in MAM.

Article Snippet: STIM1 , Stromal interaction molecule 1 , Rabbit , ACC-063-AO. Alomone Labs..

Techniques:

Journal: Frontiers in Cellular Neuroscience

Article Title: Ginkgolide B Maintains Calcium Homeostasis in Hypoxic Hippocampal Neurons by Inhibiting Calcium Influx and Intracellular Calcium Release

doi: 10.3389/fncel.2020.627846

Figure Lengend Snippet: GB reduced the expressions of proteins involved in intracellular calcium homeostasis in neurons exposed to hypoxia. (A) Representative western blots showing the expressions of Ca v 1.2, STIM1 and RyR2 proteins in hippocampal tissue from the various groups. (B) Quantification of the western blot data. (C) qPCR analysis of the mRNA levels of Ca v 1.2, STIM1 and RyR2. Datafor (B) and (C) are expressed as the mean ± SEM ( n = 3). ** P < 0.01 for hypoxia group vs. control group; # P < 0.05, ## P < 0.01 for hypoxia-GB group vs. hypoxia group (Student's t -test).

Article Snippet: The membranes were blocked for 1 h in non-fat milk and then incubated overnight at 4°C with the following primary antibodies: rabbit anti-Ca v 1.2 (1:200, cat. #ACC-005, Alomone, Jerusalem, Israel), rabbit anti-stromal interaction molecule-1 (STIM1; 1:500, cat. #ACC-063, Alomone), rabbit anti-ryanodine receptor type-1 (RyR2; 1:200, cat. #ARR-001, Alomone) and rabbit anti-β-actin (1:2,000, cat. #4970, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot