anti spink3  (Cell Signaling Technology Inc)

 
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    Name:
    SPINK3 Antibody
    Description:
    The Kazal type 3 serine peptidase inhibitor SPINK3 is a serine peptidase inhibitor expressed in the adult pancreas that protects acinar cells from trypsin induced cell damage 1 Ingestion of milk by newborn SPINK3 knock out mice triggers autophagic cell death in pancreatic acinar cells and the complete disappearance of the pancreas 2 Mutations in the human SPINK3 homolog known as SPINK1 are associated with chronic pancreatitis presumably through decreased protection against trypsin induced autophagic cell death 1 3 SPINK3 may be involved in the embryonic development of several other tissues 4
    Catalog Number:
    2744
    Price:
    None
    Applications:
    Western Blot
    Category:
    Primary Antibodies
    Source:
    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the mouse SPINK3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
    Reactivity:
    Mouse Rat
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    Structured Review

    Cell Signaling Technology Inc anti spink3
    The Kazal type 3 serine peptidase inhibitor SPINK3 is a serine peptidase inhibitor expressed in the adult pancreas that protects acinar cells from trypsin induced cell damage 1 Ingestion of milk by newborn SPINK3 knock out mice triggers autophagic cell death in pancreatic acinar cells and the complete disappearance of the pancreas 2 Mutations in the human SPINK3 homolog known as SPINK1 are associated with chronic pancreatitis presumably through decreased protection against trypsin induced autophagic cell death 1 3 SPINK3 may be involved in the embryonic development of several other tissues 4
    https://www.bioz.com/result/anti spink3/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti spink3 - by Bioz Stars, 2020-09
    90/100 stars

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  • 90
    Cell Signaling Technology Inc spink3
    Interaction of Htt with <t>Spink3</t> regulates trypsin activity in vitro. ( A ) Measurement of trypsin activity in vitro by adding Spink3, HTT, nHTT, tHTT, or Spink3 and HTT, nHTT, and tHTT containing cell lysates of transfected HEK293 cells. Cell extracts were mixed with porcine trypsin (TRSEQII) and the substrate Boc-Gln-Ala-Arg-AMC, and the rates of fluorescent products generated by trypsin were measured. Results are indicated as means ± SEM ( n = 3; two-way ANOVA test, * P
    Spink3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spink3/product/Cell Signaling Technology Inc
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    spink3 - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

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    Interaction of Htt with Spink3 regulates trypsin activity in vitro. ( A ) Measurement of trypsin activity in vitro by adding Spink3, HTT, nHTT, tHTT, or Spink3 and HTT, nHTT, and tHTT containing cell lysates of transfected HEK293 cells. Cell extracts were mixed with porcine trypsin (TRSEQII) and the substrate Boc-Gln-Ala-Arg-AMC, and the rates of fluorescent products generated by trypsin were measured. Results are indicated as means ± SEM ( n = 3; two-way ANOVA test, * P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis

    doi: 10.1073/pnas.1524575113

    Figure Lengend Snippet: Interaction of Htt with Spink3 regulates trypsin activity in vitro. ( A ) Measurement of trypsin activity in vitro by adding Spink3, HTT, nHTT, tHTT, or Spink3 and HTT, nHTT, and tHTT containing cell lysates of transfected HEK293 cells. Cell extracts were mixed with porcine trypsin (TRSEQII) and the substrate Boc-Gln-Ala-Arg-AMC, and the rates of fluorescent products generated by trypsin were measured. Results are indicated as means ± SEM ( n = 3; two-way ANOVA test, * P

    Article Snippet: Rabbit antibodies used in this study were obtained from commercial sources as follows: HTT [EPR5526] (Abcam), beta-tubulin III [EP1569Y] (Novus Biologicals), NF-κB [D14E12] (Cell Signaling), LC3I/II [NB100-2220] (Novus Biologicals), ELA3B [GTX105123] (GeneTex), FAT10 [EPR4370] (GeneTex), SPINK3 [2744S] (Cell Signaling), HA [C29F4] (Cell Signaling), p-NF-κB [93H1] (Cell Signaling), RIP3 [GTX107574] (GeneTex), Caspase3 [9662] (Cell Signaling), and cleaved Caspase3 [5A1E] (Cell Signaling).

    Techniques: Activity Assay, In Vitro, Transfection, Generated

    Interaction of Htt with Spink3 inhibits trypsin activity. ( A ) Western blotting ( Upper ) and quantification ( Lower ) of Spink3 in 2-, 4-, and 8-mo-old ubiquitous KO and control mice. ( n = 3 independent experiments, two-way ANOVA test; n.s. represents no significant difference, *** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Ablation of huntingtin in adult neurons is nondeleterious but its depletion in young mice causes acute pancreatitis

    doi: 10.1073/pnas.1524575113

    Figure Lengend Snippet: Interaction of Htt with Spink3 inhibits trypsin activity. ( A ) Western blotting ( Upper ) and quantification ( Lower ) of Spink3 in 2-, 4-, and 8-mo-old ubiquitous KO and control mice. ( n = 3 independent experiments, two-way ANOVA test; n.s. represents no significant difference, *** P

    Article Snippet: Rabbit antibodies used in this study were obtained from commercial sources as follows: HTT [EPR5526] (Abcam), beta-tubulin III [EP1569Y] (Novus Biologicals), NF-κB [D14E12] (Cell Signaling), LC3I/II [NB100-2220] (Novus Biologicals), ELA3B [GTX105123] (GeneTex), FAT10 [EPR4370] (GeneTex), SPINK3 [2744S] (Cell Signaling), HA [C29F4] (Cell Signaling), p-NF-κB [93H1] (Cell Signaling), RIP3 [GTX107574] (GeneTex), Caspase3 [9662] (Cell Signaling), and cleaved Caspase3 [5A1E] (Cell Signaling).

    Techniques: Activity Assay, Western Blot, Mouse Assay