anti sox2 ab  (Thermo Fisher)


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    Name:
    anti Sox2
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    Catalog Number:
    pa5-17282
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    Structured Review

    Thermo Fisher anti sox2 ab
    TrLp is significantly more potent than CLp in causing suppression of CD133(+) and <t>SOX2(+)</t> GL261 stem cells. Treated GL261 cells were stained with antibodies against CD133 and SOX2 and analyzed via flow cytometry. ( A , E , F ) The Vehicle-treated GL261 cells showed an abundance of CD133(+) GBM stem cells (UL quadrant, within the red rectangle). ( A – C , E , F ) Compared to the Vehicle-treated, the CLp-treated cells (UL quadrant, within the red rectangle) showed a 69% suppression of CD133 IF (* p = 1.2 × 10 −3 ) and TrLp-treated cells (UL quadrant, within the red rectangle) showed a 92% suppression of CD133(+) IF (** p = 6.9 × 10 −4 ) ( E , F ); ( F ) TrLp treatment yielded 23% greater suppression of CD133 IF than CLp (Δ p = 1.6 × 10 −3 ); ( D ) 2° antibody staining showed background fluorescence; ( G – I , K , L ) Compared to the Vehicle-treated cells, the CLp-treated cells (UL quadrant, within the red circle) showed 56% suppression of SOX2 IF (* p = 3.2 × 10 −3 ), and the TrLp-treated cells showed 82% suppression of SOX2 IF (** p = 1.0 × 10 −3 ) ( K , L ); ( L ) This inhibition was 26% greater in the TrLp group than in the CLp group (Δ p = 5.6 × 10 −3 ). Data (mean ± S.E.M.) were obtained from Vehicle ( n = 3), CLp ( n = 3) and TrLp ( n = 3); ( J ) 2° antibody-treated samples showed background staining.

    https://www.bioz.com/result/anti sox2 ab/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti sox2 ab - by Bioz Stars, 2020-07
    95/100 stars

    Images

    1) Product Images from "Liposomal TriCurin, A Synergistic Combination of Curcumin, Epicatechin Gallate and Resveratrol, Repolarizes Tumor-Associated Microglia/Macrophages, and Eliminates Glioblastoma (GBM) and GBM Stem Cells"

    Article Title: Liposomal TriCurin, A Synergistic Combination of Curcumin, Epicatechin Gallate and Resveratrol, Repolarizes Tumor-Associated Microglia/Macrophages, and Eliminates Glioblastoma (GBM) and GBM Stem Cells

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23010201

    TrLp is significantly more potent than CLp in causing suppression of CD133(+) and SOX2(+) GL261 stem cells. Treated GL261 cells were stained with antibodies against CD133 and SOX2 and analyzed via flow cytometry. ( A , E , F ) The Vehicle-treated GL261 cells showed an abundance of CD133(+) GBM stem cells (UL quadrant, within the red rectangle). ( A – C , E , F ) Compared to the Vehicle-treated, the CLp-treated cells (UL quadrant, within the red rectangle) showed a 69% suppression of CD133 IF (* p = 1.2 × 10 −3 ) and TrLp-treated cells (UL quadrant, within the red rectangle) showed a 92% suppression of CD133(+) IF (** p = 6.9 × 10 −4 ) ( E , F ); ( F ) TrLp treatment yielded 23% greater suppression of CD133 IF than CLp (Δ p = 1.6 × 10 −3 ); ( D ) 2° antibody staining showed background fluorescence; ( G – I , K , L ) Compared to the Vehicle-treated cells, the CLp-treated cells (UL quadrant, within the red circle) showed 56% suppression of SOX2 IF (* p = 3.2 × 10 −3 ), and the TrLp-treated cells showed 82% suppression of SOX2 IF (** p = 1.0 × 10 −3 ) ( K , L ); ( L ) This inhibition was 26% greater in the TrLp group than in the CLp group (Δ p = 5.6 × 10 −3 ). Data (mean ± S.E.M.) were obtained from Vehicle ( n = 3), CLp ( n = 3) and TrLp ( n = 3); ( J ) 2° antibody-treated samples showed background staining.
    Figure Legend Snippet: TrLp is significantly more potent than CLp in causing suppression of CD133(+) and SOX2(+) GL261 stem cells. Treated GL261 cells were stained with antibodies against CD133 and SOX2 and analyzed via flow cytometry. ( A , E , F ) The Vehicle-treated GL261 cells showed an abundance of CD133(+) GBM stem cells (UL quadrant, within the red rectangle). ( A – C , E , F ) Compared to the Vehicle-treated, the CLp-treated cells (UL quadrant, within the red rectangle) showed a 69% suppression of CD133 IF (* p = 1.2 × 10 −3 ) and TrLp-treated cells (UL quadrant, within the red rectangle) showed a 92% suppression of CD133(+) IF (** p = 6.9 × 10 −4 ) ( E , F ); ( F ) TrLp treatment yielded 23% greater suppression of CD133 IF than CLp (Δ p = 1.6 × 10 −3 ); ( D ) 2° antibody staining showed background fluorescence; ( G – I , K , L ) Compared to the Vehicle-treated cells, the CLp-treated cells (UL quadrant, within the red circle) showed 56% suppression of SOX2 IF (* p = 3.2 × 10 −3 ), and the TrLp-treated cells showed 82% suppression of SOX2 IF (** p = 1.0 × 10 −3 ) ( K , L ); ( L ) This inhibition was 26% greater in the TrLp group than in the CLp group (Δ p = 5.6 × 10 −3 ). Data (mean ± S.E.M.) were obtained from Vehicle ( n = 3), CLp ( n = 3) and TrLp ( n = 3); ( J ) 2° antibody-treated samples showed background staining.

    Techniques Used: Staining, Flow Cytometry, Cytometry, IF-P, Fluorescence, Inhibition

    2) Product Images from "Liposomal TriCurin, A Synergistic Combination of Curcumin, Epicatechin Gallate and Resveratrol, Repolarizes Tumor-Associated Microglia/Macrophages, and Eliminates Glioblastoma (GBM) and GBM Stem Cells"

    Article Title: Liposomal TriCurin, A Synergistic Combination of Curcumin, Epicatechin Gallate and Resveratrol, Repolarizes Tumor-Associated Microglia/Macrophages, and Eliminates Glioblastoma (GBM) and GBM Stem Cells

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    doi: 10.3390/molecules23010201

    TrLp is significantly more potent than CLp in causing suppression of CD133(+) and SOX2(+) GL261 stem cells. Treated GL261 cells were stained with antibodies against CD133 and SOX2 and analyzed via flow cytometry. ( A , E , F ) The Vehicle-treated GL261 cells showed an abundance of CD133(+) GBM stem cells (UL quadrant, within the red rectangle). ( A – C , E , F ) Compared to the Vehicle-treated, the CLp-treated cells (UL quadrant, within the red rectangle) showed a 69% suppression of CD133 IF (* p = 1.2 × 10 −3 ) and TrLp-treated cells (UL quadrant, within the red rectangle) showed a 92% suppression of CD133(+) IF (** p = 6.9 × 10 −4 ) ( E , F ); ( F ) TrLp treatment yielded 23% greater suppression of CD133 IF than CLp (Δ p = 1.6 × 10 −3 ); ( D ) 2° antibody staining showed background fluorescence; ( G – I , K , L ) Compared to the Vehicle-treated cells, the CLp-treated cells (UL quadrant, within the red circle) showed 56% suppression of SOX2 IF (* p = 3.2 × 10 −3 ), and the TrLp-treated cells showed 82% suppression of SOX2 IF (** p = 1.0 × 10 −3 ) ( K , L ); ( L ) This inhibition was 26% greater in the TrLp group than in the CLp group (Δ p = 5.6 × 10 −3 ). Data (mean ± S.E.M.) were obtained from Vehicle ( n = 3), CLp ( n = 3) and TrLp ( n = 3); ( J ) 2° antibody-treated samples showed background staining.
    Figure Legend Snippet: TrLp is significantly more potent than CLp in causing suppression of CD133(+) and SOX2(+) GL261 stem cells. Treated GL261 cells were stained with antibodies against CD133 and SOX2 and analyzed via flow cytometry. ( A , E , F ) The Vehicle-treated GL261 cells showed an abundance of CD133(+) GBM stem cells (UL quadrant, within the red rectangle). ( A – C , E , F ) Compared to the Vehicle-treated, the CLp-treated cells (UL quadrant, within the red rectangle) showed a 69% suppression of CD133 IF (* p = 1.2 × 10 −3 ) and TrLp-treated cells (UL quadrant, within the red rectangle) showed a 92% suppression of CD133(+) IF (** p = 6.9 × 10 −4 ) ( E , F ); ( F ) TrLp treatment yielded 23% greater suppression of CD133 IF than CLp (Δ p = 1.6 × 10 −3 ); ( D ) 2° antibody staining showed background fluorescence; ( G – I , K , L ) Compared to the Vehicle-treated cells, the CLp-treated cells (UL quadrant, within the red circle) showed 56% suppression of SOX2 IF (* p = 3.2 × 10 −3 ), and the TrLp-treated cells showed 82% suppression of SOX2 IF (** p = 1.0 × 10 −3 ) ( K , L ); ( L ) This inhibition was 26% greater in the TrLp group than in the CLp group (Δ p = 5.6 × 10 −3 ). Data (mean ± S.E.M.) were obtained from Vehicle ( n = 3), CLp ( n = 3) and TrLp ( n = 3); ( J ) 2° antibody-treated samples showed background staining.

    Techniques Used: Staining, Flow Cytometry, Cytometry, IF-P, Fluorescence, Inhibition

    Related Articles

    Immunohistochemistry:

    Article Title: Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage
    Article Snippet: .. For immunohistochemistry, the following primary antibodies were used in various combinations: anti-Sox10 guinea pig antiserum (1:1,000; ), anti-Oct6 rabbit antiserum (1:2,000; ), anti-Krox20 rabbit antiserum (1:200; Covance), anti-Sox2 rabbit antiserum (1:500), anti-Ki67 rabbit antiserum (1:500; Thermo Fisher Scientific), anti-CD3 rabbit antiserum (1:500; Abcam), anti-GFP rabbit antiserum (1:500; Invitrogen), anti-Iba1 rabbit antiserum (1:250; Wako Chemicals USA, Inc.) antidesmin rabbit antiserum (1:1,000; Abcam), anti–von Willebrand factor rabbit antiserum (1:800; Abcam), and anti-PECAM rat antiserum (1:200; BD). .. Secondary antibodies conjugated to Cy2, Cy3, or Alexa Fluor 488 immunofluorescent dyes (Dianova) were used for detection.

    Next-Generation Sequencing:

    Article Title: Long noncoding RNA Sox2ot and transcription factor YY1 co-regulate the differentiation of cortical neural progenitors by repressing Sox2
    Article Snippet: .. For sections stained with Sox2 primary antibody, normal donkey serum was substituted for NGS and donkey anti-rabbit IgG-Alexa-Fluor-488 and donkey anti-goat IgG-Alexa-Fluor-546 were used for visualization (1:1000, Molecular Probes). .. Primary antibodies against the following antigens were used: Sox2 (1:200, Santa Cruz), Pax6 (1:500, Covance), Tbr2 (1:500, Abcam), BrdU (1:50, DSHB), Caspase3 (1:1000, R & D Systems), Tbr1 (1:500, Abcam), Satb2 (1:500, Abcam), GFP (1:1000, Rockland, rabbit), GFP (1:1000, Abcam, chicken), and Yy1 (1:200, Santa Cruz).

    Blocking Assay:

    Article Title: Genome Editing in Patient iPSCs Corrects the Most Prevalent USH2A Mutations and Reveals Intriguing Mutant mRNA Expression Profiles
    Article Snippet: .. Primary antibodies were used at a 1:200 dilution in blocking solution and incubated overnight at 4°C: rabbit anti-NANOG (Abcam, Paris, France), mouse anti-OCT3/4 (Santa Cruz Biotechnology, Heidelberg, Germany), and rabbit anti-SOX2 (Thermo Fisher Scientific) for the iPSCs, and rabbit anti-GFAP (Dako, Les Ulis, France), mouse anti-SMA (Dako), and mouse anti-AFP (Sigma Aldrich) for the embryoid bodies. .. Fluorescence-conjugated secondary anti-mouse and anti-rabbit antibodies (Jackson ImmunoResearch Laboratories, Suffolk, UK) were used at a 1:500 dilution and incubated for 1 h at room temperature.

    Incubation:

    Article Title: Genome Editing in Patient iPSCs Corrects the Most Prevalent USH2A Mutations and Reveals Intriguing Mutant mRNA Expression Profiles
    Article Snippet: .. Primary antibodies were used at a 1:200 dilution in blocking solution and incubated overnight at 4°C: rabbit anti-NANOG (Abcam, Paris, France), mouse anti-OCT3/4 (Santa Cruz Biotechnology, Heidelberg, Germany), and rabbit anti-SOX2 (Thermo Fisher Scientific) for the iPSCs, and rabbit anti-GFAP (Dako, Les Ulis, France), mouse anti-SMA (Dako), and mouse anti-AFP (Sigma Aldrich) for the embryoid bodies. .. Fluorescence-conjugated secondary anti-mouse and anti-rabbit antibodies (Jackson ImmunoResearch Laboratories, Suffolk, UK) were used at a 1:500 dilution and incubated for 1 h at room temperature.

    Staining:

    Article Title: Long noncoding RNA Sox2ot and transcription factor YY1 co-regulate the differentiation of cortical neural progenitors by repressing Sox2
    Article Snippet: .. For sections stained with Sox2 primary antibody, normal donkey serum was substituted for NGS and donkey anti-rabbit IgG-Alexa-Fluor-488 and donkey anti-goat IgG-Alexa-Fluor-546 were used for visualization (1:1000, Molecular Probes). .. Primary antibodies against the following antigens were used: Sox2 (1:200, Santa Cruz), Pax6 (1:500, Covance), Tbr2 (1:500, Abcam), BrdU (1:50, DSHB), Caspase3 (1:1000, R & D Systems), Tbr1 (1:500, Abcam), Satb2 (1:500, Abcam), GFP (1:1000, Rockland, rabbit), GFP (1:1000, Abcam, chicken), and Yy1 (1:200, Santa Cruz).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation
    Article Snippet: .. For analysis of murine tissue and cells, primary antibodies at working concentrations were: rat anti-BrdU (1:100; Accurate Chemical, cat# YSRTMCA2060GA), rat anti-CD11b (1:50; BD Biosciences, cat# 550282), mouse anti-CD45 (1:100, BioLegend, cat# 103101), rat anti-CD4 (1:200, BD Bioscience, cat# 550278) rabbit anti Chi3l3 (1:50; Stemcell Technology, cat# 60130), rabbit anti-Doublecortin (Dcx, 1:100; Abcam, cat# ab18723), mouse anti-GFAP (1:500; BD Bioscience, cat# 610566), mouse anti-Ki76-FITC (1:100; BD Bioscience, cat# 617472), mouse anti-Map2 (1:250; Sigma-Aldrich, cat# M9942), rabbit anti-NG2 (1:100; Millipore, cat# ab5320), mouse anti-O4 (1:100; Millipore, cat# MAB345), rabbit anti-p-Pyk2 (Tyr402; 1:500, CST, cat# 3291 S), rabbit anti-p-cRaf (Ser259; 1:500, CST, cat# 9421 P), rabbit anti-Sox2 (1:500, Thermo Fisher Scientific, cat# A24339), rabbit anti-p-p38MAPK (THr180/Tyr182; 1:500, CST, cat4511 P), rabbit anti-p-PLCγ2 (Tyr1217; 1:500, CST, cat# 3871 P), rabbit anti-p-PI(3)K (Tyr458; 1:500, CST cat# 4228 P), rabbit anti-p-Erk1/2 (Thr202/Tyr204; 1:500, CST, cat# 9101), rabbit anti-p-EGFR (Tyr1068; 1:100, CST, cat# 3777). .. For the analysis of human cells, primary antibodies at working concentrations were: rat anti-MBP (1: 125, Millipore, cat# MAB386), goat anti-DCX (1:250, SantaCruz, cat# sc-8066), goat anti-SOX2 (1: 250, R & D Systems, cat# AF2018), goat anti-GFAP (1: 250, Santa Cruz, cat# sc-6170), rabbit anti-NG2 (1: 125, Millipore, cat# AB5320).

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  • 95
    Thermo Fisher rabbit anti sox2
    Genome Stability and Pluripotency of Corrected iPSC Lines (A and I) Phase-contrast images of USH2A -USH-iPSC clone B3B1 (A) and USH2A -RP-iPSC clone MS3F7 (I). (B and J) Genomic stability of USH2A -USH-iPSC B3B1 (B) and USH2A -RP-iPSC MS3F7 (J) as determined by a digital qPCR analysis of the most commonly rearranged regions reported in iPSCs. The copy number for each chromosomal position is shown with colored dots. (C–E and K–M) Pluripotency of USH2A -USH-iPSC clone B3B1 and USH2A -RP-iPSC clone MS3F7 as determined by immunostaining of the markers OCT3/4 (C and K), <t>SOX2</t> (D and L), and NANOG (E and M), respectively. Scale bars, 50 μM. (F-H and N-P) Differentiation capacity of USH2A -USH-iPSC clone B3B1 and USH2A -RP-iPSC clone MS3F7 as determined by immunostaining of the germ layer markers GFAP (ectoderm; F and N), SMA (mesoderm; G and O), and AFP (endoderm; H and P), respectively. Scale bars, 20 μM.
    Rabbit Anti Sox2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti sox2/product/Thermo Fisher
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    rabbit anti sox2 - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    93
    Thermo Fisher rat anti sox2
    Western blotting. Protein extractions from 3 LGCA and 3 HGCA EpCAM High (+) and EpCAM Low (-) cell lines were probed for OCT4 (A; 40kDa), <t>SOX2</t> (B; 40-43kDa), NANOG (C; 37-40kDa), KLF4 (D; 54kDa) and c-MYC (E; 42 57kDa). NTERA-2 cells were used as the positive control for all iPSC markers. α-tubulin (F; 50kDa) was used as a loading control. EpCAM High and EpCAM Low cell lines were also probed for their expression of EpCAM (G; bands from ~30-40kDa) and α-SMA (H; 42kDa). HepG2 cells and 3T3 cells were used as the positive control for EpCAM and α-SMA, respectively.
    Rat Anti Sox2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti sox2/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rat anti sox2 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Genome Stability and Pluripotency of Corrected iPSC Lines (A and I) Phase-contrast images of USH2A -USH-iPSC clone B3B1 (A) and USH2A -RP-iPSC clone MS3F7 (I). (B and J) Genomic stability of USH2A -USH-iPSC B3B1 (B) and USH2A -RP-iPSC MS3F7 (J) as determined by a digital qPCR analysis of the most commonly rearranged regions reported in iPSCs. The copy number for each chromosomal position is shown with colored dots. (C–E and K–M) Pluripotency of USH2A -USH-iPSC clone B3B1 and USH2A -RP-iPSC clone MS3F7 as determined by immunostaining of the markers OCT3/4 (C and K), SOX2 (D and L), and NANOG (E and M), respectively. Scale bars, 50 μM. (F-H and N-P) Differentiation capacity of USH2A -USH-iPSC clone B3B1 and USH2A -RP-iPSC clone MS3F7 as determined by immunostaining of the germ layer markers GFAP (ectoderm; F and N), SMA (mesoderm; G and O), and AFP (endoderm; H and P), respectively. Scale bars, 20 μM.

    Journal: Molecular Therapy. Methods & Clinical Development

    Article Title: Genome Editing in Patient iPSCs Corrects the Most Prevalent USH2A Mutations and Reveals Intriguing Mutant mRNA Expression Profiles

    doi: 10.1016/j.omtm.2019.11.016

    Figure Lengend Snippet: Genome Stability and Pluripotency of Corrected iPSC Lines (A and I) Phase-contrast images of USH2A -USH-iPSC clone B3B1 (A) and USH2A -RP-iPSC clone MS3F7 (I). (B and J) Genomic stability of USH2A -USH-iPSC B3B1 (B) and USH2A -RP-iPSC MS3F7 (J) as determined by a digital qPCR analysis of the most commonly rearranged regions reported in iPSCs. The copy number for each chromosomal position is shown with colored dots. (C–E and K–M) Pluripotency of USH2A -USH-iPSC clone B3B1 and USH2A -RP-iPSC clone MS3F7 as determined by immunostaining of the markers OCT3/4 (C and K), SOX2 (D and L), and NANOG (E and M), respectively. Scale bars, 50 μM. (F-H and N-P) Differentiation capacity of USH2A -USH-iPSC clone B3B1 and USH2A -RP-iPSC clone MS3F7 as determined by immunostaining of the germ layer markers GFAP (ectoderm; F and N), SMA (mesoderm; G and O), and AFP (endoderm; H and P), respectively. Scale bars, 20 μM.

    Article Snippet: Primary antibodies were used at a 1:200 dilution in blocking solution and incubated overnight at 4°C: rabbit anti-NANOG (Abcam, Paris, France), mouse anti-OCT3/4 (Santa Cruz Biotechnology, Heidelberg, Germany), and rabbit anti-SOX2 (Thermo Fisher Scientific) for the iPSCs, and rabbit anti-GFAP (Dako, Les Ulis, France), mouse anti-SMA (Dako), and mouse anti-AFP (Sigma Aldrich) for the embryoid bodies.

    Techniques: Real-time Polymerase Chain Reaction, Immunostaining

    Expression of Schwann cell markers in peripheral nerves of Sox10 ΔeSC embryos. (A–L) Immunohistochemistry (A–J) and in situ hybridizations (K and L) were performed on transverse sections of wild-type (wt; A, C, E, G, I, and K) and Sox10 ΔeSC (B, D, F, H, J, and L) sciatic nerves at 16.5 (A, B, G, and H) and 18.5 dpc (C–F and I–L) using antibodies directed against Sox2 (A–D), Krox20 (E and F), Oct6 (G–J), and an antisense riboprobe for Mbp (K and L). (C, F, H, and J) Dotted lines indicate the circumference of the sciatic nerve. Bars, 50 µm.

    Journal: The Journal of Cell Biology

    Article Title: Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage

    doi: 10.1083/jcb.200912142

    Figure Lengend Snippet: Expression of Schwann cell markers in peripheral nerves of Sox10 ΔeSC embryos. (A–L) Immunohistochemistry (A–J) and in situ hybridizations (K and L) were performed on transverse sections of wild-type (wt; A, C, E, G, I, and K) and Sox10 ΔeSC (B, D, F, H, J, and L) sciatic nerves at 16.5 (A, B, G, and H) and 18.5 dpc (C–F and I–L) using antibodies directed against Sox2 (A–D), Krox20 (E and F), Oct6 (G–J), and an antisense riboprobe for Mbp (K and L). (C, F, H, and J) Dotted lines indicate the circumference of the sciatic nerve. Bars, 50 µm.

    Article Snippet: For immunohistochemistry, the following primary antibodies were used in various combinations: anti-Sox10 guinea pig antiserum (1:1,000; ), anti-Oct6 rabbit antiserum (1:2,000; ), anti-Krox20 rabbit antiserum (1:200; Covance), anti-Sox2 rabbit antiserum (1:500), anti-Ki67 rabbit antiserum (1:500; Thermo Fisher Scientific), anti-CD3 rabbit antiserum (1:500; Abcam), anti-GFP rabbit antiserum (1:500; Invitrogen), anti-Iba1 rabbit antiserum (1:250; Wako Chemicals USA, Inc.) antidesmin rabbit antiserum (1:1,000; Abcam), anti–von Willebrand factor rabbit antiserum (1:800; Abcam), and anti-PECAM rat antiserum (1:200; BD).

    Techniques: Expressing, Immunohistochemistry, In Situ

    Postnatal development of Sox10 ΔeSC mice and their sciatic nerves. (A) Weight of wild-type (open bars) and age-matched Sox10 ΔeSC (closed bars) mice was monitored at P8, P16, P24, and P32. Data are shown as means ± SEM ( n ≥ 6 for each genotype). Statistically significant differences from wild-type controls were observed from P16 onwards (**, P ≤ 0.01; and ***, P ≤ 0.001 by Student’s t test). (B) Macroscopic appearance of P32 sciatic nerves from wild-type (wt) and Sox10 ΔeSC mice. (C) Sciatic nerve thickness was quantified for wild-type and Sox10 ΔeSC mice at P8, P16, P24, and P32 by determining the area on proximal nerve sections. At least 20 sections from three mice were used per genotype for quantification. Data are presented as mean ± SEM. Differences were statistically significant between wild type and Sox10 ΔeSC mutant from P8 onwards as determined by Student’s t test (**, P ≤ 0.01; ***, P ≤ 0.001). (D–K) Immunohistochemistry was performed on sciatic nerve sections from wild-type (D, F, H, and J) and Sox10 ΔeSC (E, G, I, and K) mice at P16 (D, E, H, and I) and P32 (F, G, J, and K) using antibodies directed against Sox2 (D–G) and Krox20 (H–K). (D, F, I, and K) Dotted lines indicate the circumference of the sciatic nerve. (L–S) In situ hybridization was performed on sciatic nerve sections from wild-type (L, N, P, and R) and Sox10 ΔeSC (M, O, Q, and S) mice at P16 (L, M, P, and Q) and P32 (N, O, R, and S) using antisense riboprobes for Mpz (L–O) and Mbp (P–S). (T–W) Myelin sheaths were visualized by PPD staining of sciatic nerve sections from wild-type (T and V) and Sox10 ΔeSC (U and W) mice at P16 (T and U) and P32 (V and W). (X and Y) Electrophysiology on sciatic nerves of Sox10 ΔeSC mice. Compound action potentials were monopolarly recorded from isolated sciatic nerves of wild-type and Sox10 ΔeSC mice ( n = 2 each). Experiments were performed on both nerves of each animal with identical results within each genotype. Representative superimposed traces are presented for both genotypes showing fast nerve conduction along myelinated fibers (X) and slow conduction along nonmyelinated fibers (Y). The arrows point to components of different conduction velocities (meters/second). Bars: (B) 1 mm; (D–S) 50 µm; (T–W) 3 µm.

    Journal: The Journal of Cell Biology

    Article Title: Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage

    doi: 10.1083/jcb.200912142

    Figure Lengend Snippet: Postnatal development of Sox10 ΔeSC mice and their sciatic nerves. (A) Weight of wild-type (open bars) and age-matched Sox10 ΔeSC (closed bars) mice was monitored at P8, P16, P24, and P32. Data are shown as means ± SEM ( n ≥ 6 for each genotype). Statistically significant differences from wild-type controls were observed from P16 onwards (**, P ≤ 0.01; and ***, P ≤ 0.001 by Student’s t test). (B) Macroscopic appearance of P32 sciatic nerves from wild-type (wt) and Sox10 ΔeSC mice. (C) Sciatic nerve thickness was quantified for wild-type and Sox10 ΔeSC mice at P8, P16, P24, and P32 by determining the area on proximal nerve sections. At least 20 sections from three mice were used per genotype for quantification. Data are presented as mean ± SEM. Differences were statistically significant between wild type and Sox10 ΔeSC mutant from P8 onwards as determined by Student’s t test (**, P ≤ 0.01; ***, P ≤ 0.001). (D–K) Immunohistochemistry was performed on sciatic nerve sections from wild-type (D, F, H, and J) and Sox10 ΔeSC (E, G, I, and K) mice at P16 (D, E, H, and I) and P32 (F, G, J, and K) using antibodies directed against Sox2 (D–G) and Krox20 (H–K). (D, F, I, and K) Dotted lines indicate the circumference of the sciatic nerve. (L–S) In situ hybridization was performed on sciatic nerve sections from wild-type (L, N, P, and R) and Sox10 ΔeSC (M, O, Q, and S) mice at P16 (L, M, P, and Q) and P32 (N, O, R, and S) using antisense riboprobes for Mpz (L–O) and Mbp (P–S). (T–W) Myelin sheaths were visualized by PPD staining of sciatic nerve sections from wild-type (T and V) and Sox10 ΔeSC (U and W) mice at P16 (T and U) and P32 (V and W). (X and Y) Electrophysiology on sciatic nerves of Sox10 ΔeSC mice. Compound action potentials were monopolarly recorded from isolated sciatic nerves of wild-type and Sox10 ΔeSC mice ( n = 2 each). Experiments were performed on both nerves of each animal with identical results within each genotype. Representative superimposed traces are presented for both genotypes showing fast nerve conduction along myelinated fibers (X) and slow conduction along nonmyelinated fibers (Y). The arrows point to components of different conduction velocities (meters/second). Bars: (B) 1 mm; (D–S) 50 µm; (T–W) 3 µm.

    Article Snippet: For immunohistochemistry, the following primary antibodies were used in various combinations: anti-Sox10 guinea pig antiserum (1:1,000; ), anti-Oct6 rabbit antiserum (1:2,000; ), anti-Krox20 rabbit antiserum (1:200; Covance), anti-Sox2 rabbit antiserum (1:500), anti-Ki67 rabbit antiserum (1:500; Thermo Fisher Scientific), anti-CD3 rabbit antiserum (1:500; Abcam), anti-GFP rabbit antiserum (1:500; Invitrogen), anti-Iba1 rabbit antiserum (1:250; Wako Chemicals USA, Inc.) antidesmin rabbit antiserum (1:1,000; Abcam), anti–von Willebrand factor rabbit antiserum (1:800; Abcam), and anti-PECAM rat antiserum (1:200; BD).

    Techniques: Mouse Assay, Mutagenesis, Immunohistochemistry, In Situ Hybridization, Staining, Isolation

    Schwann cell proliferation in sciatic nerves of Sox10 ΔeSC mice. (A and B) The absolute number of Sox2-positive cells was determined in sciatic nerves of Sox10 ΔeSC mice at P8, P16, P24, and P32 (A) and set in relation to the total number of cells (B). (C) Cell numbers were also determined for YFP-expressing cells in sciatic nerves of Dhh::Cre, Rosa26 stopfloxYFP ( Rosa26 DhhYFP ; open bars) and Dhh::Cre, Sox10 fl/fl , Rosa26 stopfloxYFP mice ( Sox10 ΔeSC , Rosa26 DhhYFP ; closed bars) at P8, P16, P24, and P32. (D–G) Coimmunohistochemistry was performed on sciatic nerve sections of Sox10 ΔeSC , Rosa26 DhhYFP mice at P8 (D), P16 (E), P24 (F), and P32 (G) using antibodies against Sox2 (red) and YFP (green). (H) Proliferation rates of wild-type (wt) and Sox10 ΔeSC Schwann cells were determined at P8, P16, P24, and P32 by determining the fraction of Ki67-positive cells among the YFP-labeled cells in Rosa26 DhhYFP (open bars) and Sox10 ΔeSC , Rosa26 DhhYFP mice (closed bars). (I) The proliferation rates of Sox10 ΔeSC Schwann cells (see H) were also used to determine the relative contribution of Schwann cells to the overall proliferation in sciatic nerves of Sox10 ΔeSC mice. For all quantifications, at least 20 sections from three mice were used per genotype. Data are presented as mean ± SEM. According to the Student’s t test, differences were statistically significant between wild type and Sox10 ΔeSC mutant as indicated (**, P ≤ 0.01; ***, P ≤ 0.001). Bars, 10 µm.

    Journal: The Journal of Cell Biology

    Article Title: Sox10 is required for Schwann cell identity and progression beyond the immature Schwann cell stage

    doi: 10.1083/jcb.200912142

    Figure Lengend Snippet: Schwann cell proliferation in sciatic nerves of Sox10 ΔeSC mice. (A and B) The absolute number of Sox2-positive cells was determined in sciatic nerves of Sox10 ΔeSC mice at P8, P16, P24, and P32 (A) and set in relation to the total number of cells (B). (C) Cell numbers were also determined for YFP-expressing cells in sciatic nerves of Dhh::Cre, Rosa26 stopfloxYFP ( Rosa26 DhhYFP ; open bars) and Dhh::Cre, Sox10 fl/fl , Rosa26 stopfloxYFP mice ( Sox10 ΔeSC , Rosa26 DhhYFP ; closed bars) at P8, P16, P24, and P32. (D–G) Coimmunohistochemistry was performed on sciatic nerve sections of Sox10 ΔeSC , Rosa26 DhhYFP mice at P8 (D), P16 (E), P24 (F), and P32 (G) using antibodies against Sox2 (red) and YFP (green). (H) Proliferation rates of wild-type (wt) and Sox10 ΔeSC Schwann cells were determined at P8, P16, P24, and P32 by determining the fraction of Ki67-positive cells among the YFP-labeled cells in Rosa26 DhhYFP (open bars) and Sox10 ΔeSC , Rosa26 DhhYFP mice (closed bars). (I) The proliferation rates of Sox10 ΔeSC Schwann cells (see H) were also used to determine the relative contribution of Schwann cells to the overall proliferation in sciatic nerves of Sox10 ΔeSC mice. For all quantifications, at least 20 sections from three mice were used per genotype. Data are presented as mean ± SEM. According to the Student’s t test, differences were statistically significant between wild type and Sox10 ΔeSC mutant as indicated (**, P ≤ 0.01; ***, P ≤ 0.001). Bars, 10 µm.

    Article Snippet: For immunohistochemistry, the following primary antibodies were used in various combinations: anti-Sox10 guinea pig antiserum (1:1,000; ), anti-Oct6 rabbit antiserum (1:2,000; ), anti-Krox20 rabbit antiserum (1:200; Covance), anti-Sox2 rabbit antiserum (1:500), anti-Ki67 rabbit antiserum (1:500; Thermo Fisher Scientific), anti-CD3 rabbit antiserum (1:500; Abcam), anti-GFP rabbit antiserum (1:500; Invitrogen), anti-Iba1 rabbit antiserum (1:250; Wako Chemicals USA, Inc.) antidesmin rabbit antiserum (1:1,000; Abcam), anti–von Willebrand factor rabbit antiserum (1:800; Abcam), and anti-PECAM rat antiserum (1:200; BD).

    Techniques: Mouse Assay, Expressing, Labeling, Mutagenesis

    Human CLPs directly promote oligodendrogenesis. Human neural stem cells (NSCs) were cultured in the presence of PBS (control), human CHI3L1 and human CHIT1 at 250 ng/ml for 14 days. a – c Cells were immunostained for MBP ( a , oligodendrocytes, green), DCX ( a , neurons, red), NG2 ( b , OPC, green), GFAP ( c , astrocyte, red), SOX2 ( d , NSC, red) and nuclear stain DAPI (blue). Scale bar, 50 μm. e Quantification of MBP + , NG2 + , GFAP + , DCX + , and SOX2 + NSCs after exposure to human Chi3L1or human Chit1. Exposure of differentiating NSCs to CHI3L1 and CHIT1 led to significant increase in oligodendrogenesis. Values are expressed as percent change to PBS-treated NSCs (data are representative of three independent experiments. one-way ANOVA with Dunnett’s multiple comparison test; mean ± s.e.m * p

    Journal: Nature Communications

    Article Title: Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation

    doi: 10.1038/s41467-018-08140-7

    Figure Lengend Snippet: Human CLPs directly promote oligodendrogenesis. Human neural stem cells (NSCs) were cultured in the presence of PBS (control), human CHI3L1 and human CHIT1 at 250 ng/ml for 14 days. a – c Cells were immunostained for MBP ( a , oligodendrocytes, green), DCX ( a , neurons, red), NG2 ( b , OPC, green), GFAP ( c , astrocyte, red), SOX2 ( d , NSC, red) and nuclear stain DAPI (blue). Scale bar, 50 μm. e Quantification of MBP + , NG2 + , GFAP + , DCX + , and SOX2 + NSCs after exposure to human Chi3L1or human Chit1. Exposure of differentiating NSCs to CHI3L1 and CHIT1 led to significant increase in oligodendrogenesis. Values are expressed as percent change to PBS-treated NSCs (data are representative of three independent experiments. one-way ANOVA with Dunnett’s multiple comparison test; mean ± s.e.m * p

    Article Snippet: For analysis of murine tissue and cells, primary antibodies at working concentrations were: rat anti-BrdU (1:100; Accurate Chemical, cat# YSRTMCA2060GA), rat anti-CD11b (1:50; BD Biosciences, cat# 550282), mouse anti-CD45 (1:100, BioLegend, cat# 103101), rat anti-CD4 (1:200, BD Bioscience, cat# 550278) rabbit anti Chi3l3 (1:50; Stemcell Technology, cat# 60130), rabbit anti-Doublecortin (Dcx, 1:100; Abcam, cat# ab18723), mouse anti-GFAP (1:500; BD Bioscience, cat# 610566), mouse anti-Ki76-FITC (1:100; BD Bioscience, cat# 617472), mouse anti-Map2 (1:250; Sigma-Aldrich, cat# M9942), rabbit anti-NG2 (1:100; Millipore, cat# ab5320), mouse anti-O4 (1:100; Millipore, cat# MAB345), rabbit anti-p-Pyk2 (Tyr402; 1:500, CST, cat# 3291 S), rabbit anti-p-cRaf (Ser259; 1:500, CST, cat# 9421 P), rabbit anti-Sox2 (1:500, Thermo Fisher Scientific, cat# A24339), rabbit anti-p-p38MAPK (THr180/Tyr182; 1:500, CST, cat#4511 P), rabbit anti-p-PLCγ2 (Tyr1217; 1:500, CST, cat# 3871 P), rabbit anti-p-PI(3)K (Tyr458; 1:500, CST cat# 4228 P), rabbit anti-p-Erk1/2 (Thr202/Tyr204; 1:500, CST, cat# 9101), rabbit anti-p-EGFR (Tyr1068; 1:100, CST, cat# 3777).

    Techniques: Cell Culture, Staining

    Chi3l3 directly promotes oligodendrogenesis in vitro. Representative confocal image (above) and quantification (below) of neural stem cells (NSCs) cultured in the presence of PBS (control) or Chi3l3 (100 ng/ml) for 3 days a – d , 5 days e – h , or primary OPCs cultured in the presence of PBS (control) or Chi3l3 (500 ng/ml) for 7 days i . Cells were treated with the nuclear stain TO-PRO-3 (blue) and immunostained for early progenitor markers NG2 ( a ; oligodendrocyte precursor cells, green), GFAP ( b ; astrocytes, green), Dcx ( c ; neuroblasts, green), late progenitor markers O4 ( e ; oligodendrocytes, green), GFAP ( f ; astrocytes, green) and microtubule-associated protein 2 ( g ; Map2, neurons, green), the neural stem cell marker Sox2 ( d , h ; green) and the myelin protein MBP ( i , oligodendrocytes, green). Exposure of differentiating NSCs to Chi3l3 led to significant increase in oligodendrocyte precursor cells and oligodendrocytes, significant decrease in astrocytes, neuroblasts, and neurons and a significant increase in Sox2 + neural stem cells. Scale bar, 50 μm. Inserts show representative cells. Scale bar, 20 μm. j – n Gene expression of Cspg4 (NG2; j ), Gfap k , and Map2 ( l ; 3 days) and Ccnd1 and Ccnd2 ( m , n ; 24 h) mRNA in PBS (control) or Chi3l3-treated differentiating NSCs. Values were normalized against Gapdh (AU, arbitrary unit; n.s., not significant;). Number o and size p of neurospheres from NSCs exposed to Chi3l3 or PBS (control). (n.s., not significant; two-tailed Student’s t test; data are representative of three independent experiments with n = 5 replicates a , c , d , i , n = 9 (Control) and 10 (Chi3l3) replicates b , n = 12 (Control) and 9 (Chi3l3) replicates e , n = 12 replicates f , g , n = 3 replicates h , j , l , m , n ), n = 3 (Control) and 2 (Chi3l3) replicates k , n = 4 replicates o , n = 8 (control) and four (Chi3l3) replicates p ). mean ± s.e.m. * p

    Journal: Nature Communications

    Article Title: Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation

    doi: 10.1038/s41467-018-08140-7

    Figure Lengend Snippet: Chi3l3 directly promotes oligodendrogenesis in vitro. Representative confocal image (above) and quantification (below) of neural stem cells (NSCs) cultured in the presence of PBS (control) or Chi3l3 (100 ng/ml) for 3 days a – d , 5 days e – h , or primary OPCs cultured in the presence of PBS (control) or Chi3l3 (500 ng/ml) for 7 days i . Cells were treated with the nuclear stain TO-PRO-3 (blue) and immunostained for early progenitor markers NG2 ( a ; oligodendrocyte precursor cells, green), GFAP ( b ; astrocytes, green), Dcx ( c ; neuroblasts, green), late progenitor markers O4 ( e ; oligodendrocytes, green), GFAP ( f ; astrocytes, green) and microtubule-associated protein 2 ( g ; Map2, neurons, green), the neural stem cell marker Sox2 ( d , h ; green) and the myelin protein MBP ( i , oligodendrocytes, green). Exposure of differentiating NSCs to Chi3l3 led to significant increase in oligodendrocyte precursor cells and oligodendrocytes, significant decrease in astrocytes, neuroblasts, and neurons and a significant increase in Sox2 + neural stem cells. Scale bar, 50 μm. Inserts show representative cells. Scale bar, 20 μm. j – n Gene expression of Cspg4 (NG2; j ), Gfap k , and Map2 ( l ; 3 days) and Ccnd1 and Ccnd2 ( m , n ; 24 h) mRNA in PBS (control) or Chi3l3-treated differentiating NSCs. Values were normalized against Gapdh (AU, arbitrary unit; n.s., not significant;). Number o and size p of neurospheres from NSCs exposed to Chi3l3 or PBS (control). (n.s., not significant; two-tailed Student’s t test; data are representative of three independent experiments with n = 5 replicates a , c , d , i , n = 9 (Control) and 10 (Chi3l3) replicates b , n = 12 (Control) and 9 (Chi3l3) replicates e , n = 12 replicates f , g , n = 3 replicates h , j , l , m , n ), n = 3 (Control) and 2 (Chi3l3) replicates k , n = 4 replicates o , n = 8 (control) and four (Chi3l3) replicates p ). mean ± s.e.m. * p

    Article Snippet: For analysis of murine tissue and cells, primary antibodies at working concentrations were: rat anti-BrdU (1:100; Accurate Chemical, cat# YSRTMCA2060GA), rat anti-CD11b (1:50; BD Biosciences, cat# 550282), mouse anti-CD45 (1:100, BioLegend, cat# 103101), rat anti-CD4 (1:200, BD Bioscience, cat# 550278) rabbit anti Chi3l3 (1:50; Stemcell Technology, cat# 60130), rabbit anti-Doublecortin (Dcx, 1:100; Abcam, cat# ab18723), mouse anti-GFAP (1:500; BD Bioscience, cat# 610566), mouse anti-Ki76-FITC (1:100; BD Bioscience, cat# 617472), mouse anti-Map2 (1:250; Sigma-Aldrich, cat# M9942), rabbit anti-NG2 (1:100; Millipore, cat# ab5320), mouse anti-O4 (1:100; Millipore, cat# MAB345), rabbit anti-p-Pyk2 (Tyr402; 1:500, CST, cat# 3291 S), rabbit anti-p-cRaf (Ser259; 1:500, CST, cat# 9421 P), rabbit anti-Sox2 (1:500, Thermo Fisher Scientific, cat# A24339), rabbit anti-p-p38MAPK (THr180/Tyr182; 1:500, CST, cat#4511 P), rabbit anti-p-PLCγ2 (Tyr1217; 1:500, CST, cat# 3871 P), rabbit anti-p-PI(3)K (Tyr458; 1:500, CST cat# 4228 P), rabbit anti-p-Erk1/2 (Thr202/Tyr204; 1:500, CST, cat# 9101), rabbit anti-p-EGFR (Tyr1068; 1:100, CST, cat# 3777).

    Techniques: In Vitro, Cell Culture, Staining, Marker, Expressing, Two Tailed Test

    Western blotting. Protein extractions from 3 LGCA and 3 HGCA EpCAM High (+) and EpCAM Low (-) cell lines were probed for OCT4 (A; 40kDa), SOX2 (B; 40-43kDa), NANOG (C; 37-40kDa), KLF4 (D; 54kDa) and c-MYC (E; 42 57kDa). NTERA-2 cells were used as the positive control for all iPSC markers. α-tubulin (F; 50kDa) was used as a loading control. EpCAM High and EpCAM Low cell lines were also probed for their expression of EpCAM (G; bands from ~30-40kDa) and α-SMA (H; 42kDa). HepG2 cells and 3T3 cells were used as the positive control for EpCAM and α-SMA, respectively.

    Journal: PLoS ONE

    Article Title: Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function

    doi: 10.1371/journal.pone.0232934

    Figure Lengend Snippet: Western blotting. Protein extractions from 3 LGCA and 3 HGCA EpCAM High (+) and EpCAM Low (-) cell lines were probed for OCT4 (A; 40kDa), SOX2 (B; 40-43kDa), NANOG (C; 37-40kDa), KLF4 (D; 54kDa) and c-MYC (E; 42 57kDa). NTERA-2 cells were used as the positive control for all iPSC markers. α-tubulin (F; 50kDa) was used as a loading control. EpCAM High and EpCAM Low cell lines were also probed for their expression of EpCAM (G; bands from ~30-40kDa) and α-SMA (H; 42kDa). HepG2 cells and 3T3 cells were used as the positive control for EpCAM and α-SMA, respectively.

    Article Snippet: Primary antibodies included rabbit anti-OCT4 (cat# A24867, Thermo), rat anti-SOX2 (cat# A24759, Thermo), mouse IgG3 anti-SSEA4 (cat# A24866, Thermo) and mouse IgM anti-TRA-1-60 (cat# A24868, Thermo).

    Techniques: Western Blot, Positive Control, Expressing

    RT-qPCR. RNA was extracted from EpCAM High and EpCAM Low cells lines from 3 LGCA and 3 HGCA cases, and RT-qPCR was carried out to measure the mRNA levels of OCT4 (A), SOX2 (B), NANOG (C), KLF4 (D) and c-MYC (E). Triplicate values are shown by dots (EpCAM Low ) and squares (EpCAM High ), with mean and 95% confidence intervals. Abundance was measured relative to qPCR Human Reference Total RNA (Mediray). LGCA (n = 3); HGCA (n = 3).

    Journal: PLoS ONE

    Article Title: Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function

    doi: 10.1371/journal.pone.0232934

    Figure Lengend Snippet: RT-qPCR. RNA was extracted from EpCAM High and EpCAM Low cells lines from 3 LGCA and 3 HGCA cases, and RT-qPCR was carried out to measure the mRNA levels of OCT4 (A), SOX2 (B), NANOG (C), KLF4 (D) and c-MYC (E). Triplicate values are shown by dots (EpCAM Low ) and squares (EpCAM High ), with mean and 95% confidence intervals. Abundance was measured relative to qPCR Human Reference Total RNA (Mediray). LGCA (n = 3); HGCA (n = 3).

    Article Snippet: Primary antibodies included rabbit anti-OCT4 (cat# A24867, Thermo), rat anti-SOX2 (cat# A24759, Thermo), mouse IgG3 anti-SSEA4 (cat# A24866, Thermo) and mouse IgM anti-TRA-1-60 (cat# A24868, Thermo).

    Techniques: Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    Densitometry performed on western blot. Densitometry data provided semi-quantitative data for protein abundance. The intensity values of all LGCA cell lines (both EpCAM High and EpCAM Low ) and all HGCA cell lines (both EpCAM High and EpCAM Low ) were combined and the average intensity calculated, and these are shown for OCT4 (A), SOX2 (C), NANOG (E), KLF4 (G) and c-MYC (I). The intensity values of all EpCAM Low cell lines (both LGCA and HGCA) and all EpCAM High cell lines (both LGCA and HGCA) were combined and the average intensity calculated, and these are shown for OCT4 (B), SOX2 (D), NANOG (F), KLF4 (H), c-MYC (J), EpCAM (K) and α-SMA (L). Individual intensity values were normalized against the loading control α-tubulin before being combined and averaged. Error bars show standard deviation.

    Journal: PLoS ONE

    Article Title: Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function

    doi: 10.1371/journal.pone.0232934

    Figure Lengend Snippet: Densitometry performed on western blot. Densitometry data provided semi-quantitative data for protein abundance. The intensity values of all LGCA cell lines (both EpCAM High and EpCAM Low ) and all HGCA cell lines (both EpCAM High and EpCAM Low ) were combined and the average intensity calculated, and these are shown for OCT4 (A), SOX2 (C), NANOG (E), KLF4 (G) and c-MYC (I). The intensity values of all EpCAM Low cell lines (both LGCA and HGCA) and all EpCAM High cell lines (both LGCA and HGCA) were combined and the average intensity calculated, and these are shown for OCT4 (B), SOX2 (D), NANOG (F), KLF4 (H), c-MYC (J), EpCAM (K) and α-SMA (L). Individual intensity values were normalized against the loading control α-tubulin before being combined and averaged. Error bars show standard deviation.

    Article Snippet: Primary antibodies included rabbit anti-OCT4 (cat# A24867, Thermo), rat anti-SOX2 (cat# A24759, Thermo), mouse IgG3 anti-SSEA4 (cat# A24866, Thermo) and mouse IgM anti-TRA-1-60 (cat# A24868, Thermo).

    Techniques: Western Blot, Standard Deviation

    Representative immunofluorescence immunocytochemical images. EpCAM Low (A) and EpCAM High (B) cells from low-grade colon adenocarcinoma (LGCA)-derived primary cell lines, and EpCAM Low (C) and EpCAM High (D) cells from high-grade colon adenocarcinoma (HGCA)-derived primary cell lines showing expression of SOX2 (green) and TRA-1-60 (red). LGCA (n = 3); HGCA (n = 3). Positive control NTERA-2 (E) and CaCo2 (F) cells were stained for SOX2 (green) and TRA-1-60 (red) (B). Original magnification: 400x; scale bar = 20 μm.

    Journal: PLoS ONE

    Article Title: Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function

    doi: 10.1371/journal.pone.0232934

    Figure Lengend Snippet: Representative immunofluorescence immunocytochemical images. EpCAM Low (A) and EpCAM High (B) cells from low-grade colon adenocarcinoma (LGCA)-derived primary cell lines, and EpCAM Low (C) and EpCAM High (D) cells from high-grade colon adenocarcinoma (HGCA)-derived primary cell lines showing expression of SOX2 (green) and TRA-1-60 (red). LGCA (n = 3); HGCA (n = 3). Positive control NTERA-2 (E) and CaCo2 (F) cells were stained for SOX2 (green) and TRA-1-60 (red) (B). Original magnification: 400x; scale bar = 20 μm.

    Article Snippet: Primary antibodies included rabbit anti-OCT4 (cat# A24867, Thermo), rat anti-SOX2 (cat# A24759, Thermo), mouse IgG3 anti-SSEA4 (cat# A24866, Thermo) and mouse IgM anti-TRA-1-60 (cat# A24868, Thermo).

    Techniques: Immunofluorescence, Derivative Assay, Expressing, Positive Control, Staining