Structured Review

Cayman Chemical anti s1p1
Representative immunofluorescence of sphingosine-1-phosphate receptor-1 <t>(S1P1;</t> A ), neuronal nuclei (NeuN; B ), terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling (TUNEL; C ), and colocalizations of S1P1+NeuN
Anti S1p1, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti s1p1/product/Cayman Chemical
Average 90 stars, based on 4 article reviews
Price from $9.99 to $1999.99
anti s1p1 - by Bioz Stars, 2022-10
90/100 stars

Images

1) Product Images from "Role of the Sphingosine Metabolism Pathway on Neurons against Experimental Cerebral Ischemia in Rats"

Article Title: Role of the Sphingosine Metabolism Pathway on Neurons against Experimental Cerebral Ischemia in Rats

Journal: Translational stroke research

doi: 10.1007/s12975-013-0260-7

Representative immunofluorescence of sphingosine-1-phosphate receptor-1 (S1P1; A ), neuronal nuclei (NeuN; B ), terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling (TUNEL; C ), and colocalizations of S1P1+NeuN
Figure Legend Snippet: Representative immunofluorescence of sphingosine-1-phosphate receptor-1 (S1P1; A ), neuronal nuclei (NeuN; B ), terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling (TUNEL; C ), and colocalizations of S1P1+NeuN

Techniques Used: Immunofluorescence, End Labeling, TUNEL Assay

Representative immunofluorescence of colocalizations (arrow) between neuronal nuclei (NeuN) and sphingosine-1-phosphate receptor-1 (S1P1; A ), sphingosine kinase 1 (SphK1; B ), or sphingosine kinase 2 (SphK2; C ) in infarct (I) and periinfarct (P) cortices
Figure Legend Snippet: Representative immunofluorescence of colocalizations (arrow) between neuronal nuclei (NeuN) and sphingosine-1-phosphate receptor-1 (S1P1; A ), sphingosine kinase 1 (SphK1; B ), or sphingosine kinase 2 (SphK2; C ) in infarct (I) and periinfarct (P) cortices

Techniques Used: Immunofluorescence

Changes in sphingosine-1-phosphate receptor-1 (S1P1), sphingosine kinase 1 (SphK1), and sphingosine kinase 2 (SphK2) expressions in the middle cerebral artery (MCA) region including infarct (I) and periinfarct (P) cortices at preoperation (pre-op.), 6
Figure Legend Snippet: Changes in sphingosine-1-phosphate receptor-1 (S1P1), sphingosine kinase 1 (SphK1), and sphingosine kinase 2 (SphK2) expressions in the middle cerebral artery (MCA) region including infarct (I) and periinfarct (P) cortices at preoperation (pre-op.), 6

Techniques Used:

2) Product Images from "Role of the Sphingosine Metabolism Pathway on Neurons against Experimental Cerebral Ischemia in Rats"

Article Title: Role of the Sphingosine Metabolism Pathway on Neurons against Experimental Cerebral Ischemia in Rats

Journal: Translational stroke research

doi: 10.1007/s12975-013-0260-7

Representative immunofluorescence of sphingosine-1-phosphate receptor-1 (S1P1; A ), neuronal nuclei (NeuN; B ), terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling (TUNEL; C ), and colocalizations of S1P1+NeuN
Figure Legend Snippet: Representative immunofluorescence of sphingosine-1-phosphate receptor-1 (S1P1; A ), neuronal nuclei (NeuN; B ), terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick end-labeling (TUNEL; C ), and colocalizations of S1P1+NeuN

Techniques Used: Immunofluorescence, End Labeling, TUNEL Assay

Representative immunofluorescence of colocalizations (arrow) between neuronal nuclei (NeuN) and sphingosine-1-phosphate receptor-1 (S1P1; A ), sphingosine kinase 1 (SphK1; B ), or sphingosine kinase 2 (SphK2; C ) in infarct (I) and periinfarct (P) cortices
Figure Legend Snippet: Representative immunofluorescence of colocalizations (arrow) between neuronal nuclei (NeuN) and sphingosine-1-phosphate receptor-1 (S1P1; A ), sphingosine kinase 1 (SphK1; B ), or sphingosine kinase 2 (SphK2; C ) in infarct (I) and periinfarct (P) cortices

Techniques Used: Immunofluorescence

Changes in sphingosine-1-phosphate receptor-1 (S1P1), sphingosine kinase 1 (SphK1), and sphingosine kinase 2 (SphK2) expressions in the middle cerebral artery (MCA) region including infarct (I) and periinfarct (P) cortices at preoperation (pre-op.), 6
Figure Legend Snippet: Changes in sphingosine-1-phosphate receptor-1 (S1P1), sphingosine kinase 1 (SphK1), and sphingosine kinase 2 (SphK2) expressions in the middle cerebral artery (MCA) region including infarct (I) and periinfarct (P) cortices at preoperation (pre-op.), 6

Techniques Used:

3) Product Images from "Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors"

Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

Journal: Journal of Ovarian Research

doi: 10.1186/1757-2215-4-4

S1P1 receptor expression in human ovarian carcinomas . (A) Normal ovary showing diffusely stained S1P+ stromal cells (white arrows) and a blood vessel (BV) with intensely stained S1P1+ endothelial cells (black arrow) (200×). (B) Serous ovarian tumor with S1P1+ stroma (white arrows) and unstained surface epithelium (200×). (C) blood vessels in normal ovary (BV, arrows) are S1P1 + (600×). (D) S1P1+ endometrioid ovarian tumor with patches of S1P1+staining within the surrounding stroma (white arrows) adjacent to unstained endometrioid tumor (400×). (A-D) are paraffin-embedded sections.
Figure Legend Snippet: S1P1 receptor expression in human ovarian carcinomas . (A) Normal ovary showing diffusely stained S1P+ stromal cells (white arrows) and a blood vessel (BV) with intensely stained S1P1+ endothelial cells (black arrow) (200×). (B) Serous ovarian tumor with S1P1+ stroma (white arrows) and unstained surface epithelium (200×). (C) blood vessels in normal ovary (BV, arrows) are S1P1 + (600×). (D) S1P1+ endometrioid ovarian tumor with patches of S1P1+staining within the surrounding stroma (white arrows) adjacent to unstained endometrioid tumor (400×). (A-D) are paraffin-embedded sections.

Techniques Used: Expressing, Staining

In ovarian tumors S1P1 expression was observed near T and B cells . Alternate serial sections of a serous ovarian tumor of the hen showing ( A) Bu1a+ cells in the stroma (arrows) and diffuse tissue stain (open arrow), ( B) CD4 T cells around tumor glands (arrows), ( C) S1P1 expression on the epithelium of tumor glands (arrow) and ( D) CD8 T cells (arrows) in the stroma (original magnification 100×).
Figure Legend Snippet: In ovarian tumors S1P1 expression was observed near T and B cells . Alternate serial sections of a serous ovarian tumor of the hen showing ( A) Bu1a+ cells in the stroma (arrows) and diffuse tissue stain (open arrow), ( B) CD4 T cells around tumor glands (arrows), ( C) S1P1 expression on the epithelium of tumor glands (arrow) and ( D) CD8 T cells (arrows) in the stroma (original magnification 100×).

Techniques Used: Expressing, Staining

S1P1 protein expression in hen and human tissue . S1P1 immunoreactions are similar in hen and human ovaries and ovarian tumors. Three bands at 47, 72, 108 kDa were observed. The band at 47 kDa was faint, while bands at 72 and 108 kDa were consistently present in all tissues but vary in intensity. The 47 kD band was not significantly enhanced using a membrane enriched (18,000 × g pellet) fraction. The pattern of immunoreactive bands was identical in the positive control recommended by the manufacturer (rat brain) and in hen brain and spleen. The bands were absent in control incubations in which the primary antibody was pre-adsorbed with a blocking peptide or in which the primary antibody was omitted.
Figure Legend Snippet: S1P1 protein expression in hen and human tissue . S1P1 immunoreactions are similar in hen and human ovaries and ovarian tumors. Three bands at 47, 72, 108 kDa were observed. The band at 47 kDa was faint, while bands at 72 and 108 kDa were consistently present in all tissues but vary in intensity. The 47 kD band was not significantly enhanced using a membrane enriched (18,000 × g pellet) fraction. The pattern of immunoreactive bands was identical in the positive control recommended by the manufacturer (rat brain) and in hen brain and spleen. The bands were absent in control incubations in which the primary antibody was pre-adsorbed with a blocking peptide or in which the primary antibody was omitted.

Techniques Used: Expressing, Positive Control, Blocking Assay

Localization of S1P1 receptor protein expression in normal hen ovary . ( A ) S1P1+ cells (black arrows) in theca of a mature follicle (F) and within small blood vessels (BV; white arrows) in ovarian stroma (100×). Primordial follicles (f) have comparatively little S1P1+ expression. ( B ) Endothelial cells of blood vessels (BV) in the theca externa (TE) of a follicle (F) and ovarian stroma are S1P1+ (200×). ( C ) Surface epithelial cells (EpC) showing intense S1P1+ expression (400×). ( D ) An atretic follicle (af) with characteristic infiltrating S1P1+ immune cells (100×). Inset: High magnification of (D) showing an S1P1+ immune cell (1000×). ( E ) Well developed blood vessels (BV) in the medullary region of the ovary also contain S1P1+ endothelial cells (400×) but staining is lighter and more diffuse than in stromal blood vessels seen in (A) and (B). Inset: high magnification (800×) shows detail of smooth muscle cells (SmC) and endothelial cells (EnC). ( A-C ) are frozen tissues; ( D ) and ( E ) are paraffin-embedded.
Figure Legend Snippet: Localization of S1P1 receptor protein expression in normal hen ovary . ( A ) S1P1+ cells (black arrows) in theca of a mature follicle (F) and within small blood vessels (BV; white arrows) in ovarian stroma (100×). Primordial follicles (f) have comparatively little S1P1+ expression. ( B ) Endothelial cells of blood vessels (BV) in the theca externa (TE) of a follicle (F) and ovarian stroma are S1P1+ (200×). ( C ) Surface epithelial cells (EpC) showing intense S1P1+ expression (400×). ( D ) An atretic follicle (af) with characteristic infiltrating S1P1+ immune cells (100×). Inset: High magnification of (D) showing an S1P1+ immune cell (1000×). ( E ) Well developed blood vessels (BV) in the medullary region of the ovary also contain S1P1+ endothelial cells (400×) but staining is lighter and more diffuse than in stromal blood vessels seen in (A) and (B). Inset: high magnification (800×) shows detail of smooth muscle cells (SmC) and endothelial cells (EnC). ( A-C ) are frozen tissues; ( D ) and ( E ) are paraffin-embedded.

Techniques Used: Expressing, Staining

Localization of S1P1 receptor protein expression in hen ovarian tumors . ( A ) An example of a mucinous ovarian tumor with S1P1+ staining associated with surface epithelium (black arrow) and mucin-secreting structures (black arrow) (100×). ( B ) H E stained section (100×) serial to that in (A) shows mucinous histology. ( C ) Higher magnification of (A) showing S1P1+ mucin-secreting glandular (MG) structures (black arrows) (400×). ( D ) H E stained section of a serous ovarian tumor (100×). ( E ) Serial section showing minimal stromal cell stain for S1P1+ but intense surface epithelial cell staining (black arrow) and lighter more diffuse S1P1+ sub-epithelial cells (white arrow) (100×). ( F ) High magnification (of box) showing S1P1+ surface epithelial cells (EpC) (600×). ( G ) Clear-cell ovarian tumor (T; left of dotted red line) with negligible S1P1+ in tumor and S1P1+ cells in adjacent uninvolved stroma (100×). ( H ) H E-stained serial section from the same tumor region in (G) shows cellular detail of clear cell carcinoma (400×). ( I ) Higher magnification of box in(G) showing stromal blood vessels (BV) with S1P1+ endothelial cells and stromal cells (S) (200×). ( J ) H E stained section of late stage endometrioid tumor (100×). ( K ) S1P1+ is highly expressed in cells in the tumor periphery and to a lesser extent in tumor stroma (100×). ( L ) High magnification of box in (K) showing cytoplasmic staining of endometrioid tumor cells (400x). All images are from paraffin-embedded tissue, except clear cell carcinoma (G-I) which is a frozen section.
Figure Legend Snippet: Localization of S1P1 receptor protein expression in hen ovarian tumors . ( A ) An example of a mucinous ovarian tumor with S1P1+ staining associated with surface epithelium (black arrow) and mucin-secreting structures (black arrow) (100×). ( B ) H E stained section (100×) serial to that in (A) shows mucinous histology. ( C ) Higher magnification of (A) showing S1P1+ mucin-secreting glandular (MG) structures (black arrows) (400×). ( D ) H E stained section of a serous ovarian tumor (100×). ( E ) Serial section showing minimal stromal cell stain for S1P1+ but intense surface epithelial cell staining (black arrow) and lighter more diffuse S1P1+ sub-epithelial cells (white arrow) (100×). ( F ) High magnification (of box) showing S1P1+ surface epithelial cells (EpC) (600×). ( G ) Clear-cell ovarian tumor (T; left of dotted red line) with negligible S1P1+ in tumor and S1P1+ cells in adjacent uninvolved stroma (100×). ( H ) H E-stained serial section from the same tumor region in (G) shows cellular detail of clear cell carcinoma (400×). ( I ) Higher magnification of box in(G) showing stromal blood vessels (BV) with S1P1+ endothelial cells and stromal cells (S) (200×). ( J ) H E stained section of late stage endometrioid tumor (100×). ( K ) S1P1+ is highly expressed in cells in the tumor periphery and to a lesser extent in tumor stroma (100×). ( L ) High magnification of box in (K) showing cytoplasmic staining of endometrioid tumor cells (400x). All images are from paraffin-embedded tissue, except clear cell carcinoma (G-I) which is a frozen section.

Techniques Used: Expressing, Staining

In normal hen ovary S1P1 expression was observed in areas of immune cell infiltration . ( Row A) Immune cells (Bu1a+ and CD3+) are adjacent to the follicle (f) in the ovarian stroma near a transverse blood vessel (arrow in column S1P1). Cells lining the vessel near the follicle (f) are S1P1+ (row A, S1P1) (original magnification 100×). ( Row B) High magnification (see red boxes in row A) showing B and T cells clustered within the stroma near S1P1 stained vascular endothelium (arrow). Some immune cells are also S1P1+ (open arrow) (original magnification 400×). ( Row C) Cross-section of a large blood vessel shows Bu1a and CD3 positive cells are clustered near the blood vessel. The apical surfaces of endothelial cell express S1P1 (original magnification 100×).
Figure Legend Snippet: In normal hen ovary S1P1 expression was observed in areas of immune cell infiltration . ( Row A) Immune cells (Bu1a+ and CD3+) are adjacent to the follicle (f) in the ovarian stroma near a transverse blood vessel (arrow in column S1P1). Cells lining the vessel near the follicle (f) are S1P1+ (row A, S1P1) (original magnification 100×). ( Row B) High magnification (see red boxes in row A) showing B and T cells clustered within the stroma near S1P1 stained vascular endothelium (arrow). Some immune cells are also S1P1+ (open arrow) (original magnification 400×). ( Row C) Cross-section of a large blood vessel shows Bu1a and CD3 positive cells are clustered near the blood vessel. The apical surfaces of endothelial cell express S1P1 (original magnification 100×).

Techniques Used: Expressing, Staining

S1P1 mRNA expression in hen tissues . ( A ) S1P1 mRNA (226 bp) is expressed in both normal and tumor ovaries. Examples of mRNA in tumors with endometrioid (En), serous (Sr), and mucinous (Mc) histology are shown. ( B ) Examples of other hen tissues that express S1P1 mRNA (226 bp) include liver (LVR), kidney (KDNY), skeletal muscle (MSCL), oviduct (OVDT) and spleen. Normal ovary and spleen are from the same hens (1-4). β-actin (300 bp) was used as a loading control. Controls for S1P1 primer include the positive control (+) lane which was the sample from an earlier experiment used to verify the RNA sequence. The negative control lane omitted the cDNA.
Figure Legend Snippet: S1P1 mRNA expression in hen tissues . ( A ) S1P1 mRNA (226 bp) is expressed in both normal and tumor ovaries. Examples of mRNA in tumors with endometrioid (En), serous (Sr), and mucinous (Mc) histology are shown. ( B ) Examples of other hen tissues that express S1P1 mRNA (226 bp) include liver (LVR), kidney (KDNY), skeletal muscle (MSCL), oviduct (OVDT) and spleen. Normal ovary and spleen are from the same hens (1-4). β-actin (300 bp) was used as a loading control. Controls for S1P1 primer include the positive control (+) lane which was the sample from an earlier experiment used to verify the RNA sequence. The negative control lane omitted the cDNA.

Techniques Used: Expressing, Positive Control, Sequencing, Negative Control

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    Cayman Chemical anti s1p1 antibody
    Representative ischemic brain and the area which is defined as periinfarct cortex (A) (stained by NeuN, I, infarct area; N, non-infarct area). Immunohistochemical colocalization of sphingosine-1-phosphate receptor-1 <t>(S1P1;</t> red, B) or phosphorylated Akt
    Anti S1p1 Antibody, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti s1p1 antibody/product/Cayman Chemical
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti s1p1 antibody - by Bioz Stars, 2022-10
    90/100 stars
      Buy from Supplier

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    Representative ischemic brain and the area which is defined as periinfarct cortex (A) (stained by NeuN, I, infarct area; N, non-infarct area). Immunohistochemical colocalization of sphingosine-1-phosphate receptor-1 (S1P1; red, B) or phosphorylated Akt

    Journal:

    Article Title: Activation of sphingosine 1-phosphate receptor-1 by FTY720 is neuroprotective after ischemic stroke in rats

    doi: 10.1161/STROKEAHA.109.568899

    Figure Lengend Snippet: Representative ischemic brain and the area which is defined as periinfarct cortex (A) (stained by NeuN, I, infarct area; N, non-infarct area). Immunohistochemical colocalization of sphingosine-1-phosphate receptor-1 (S1P1; red, B) or phosphorylated Akt

    Article Snippet: The following primary antibodies were used: 1) anti-S1P1 antibody (1:100, Cayman Chemical, Ann Arbor, MI), 2) anti-phospho-Akt (Ser473) antibody IHC-specific (1:100, Cell Signaling Technology, Danvers, MA), and 3) anti-NeuN antibody (1:200; Chemicon International, Temecula, CA).

    Techniques: Staining, Immunohistochemistry

    The expression of S1P1 receptor protein in ASC-differentiated to endothelial-like cells. Panel A : Western-blot for S1P1 and β-actin in four cell lines of ASC-differentiated endothelial cells. Panel B : Immunostaining for S1P1 and β-actin in ASC-differentiated to endothelial cells. Red fluorescence is for S1P1, green fluorescence is for β-actin, blue fluorescence is DAPI for nuclei. White arrows show the membrane distribution of S1P1. Western-blot coupled to immunostaining confirmed the expression of S1P1 in ASC-differentiated to endothelial-like cells.

    Journal: Journal of Biomedical Science

    Article Title: Sphingosine-1-phosphate promotes the differentiation of adipose-derived stem cells into endothelial nitric oxide synthase (eNOS) expressing endothelial-like cells

    doi: 10.1186/1423-0127-21-55

    Figure Lengend Snippet: The expression of S1P1 receptor protein in ASC-differentiated to endothelial-like cells. Panel A : Western-blot for S1P1 and β-actin in four cell lines of ASC-differentiated endothelial cells. Panel B : Immunostaining for S1P1 and β-actin in ASC-differentiated to endothelial cells. Red fluorescence is for S1P1, green fluorescence is for β-actin, blue fluorescence is DAPI for nuclei. White arrows show the membrane distribution of S1P1. Western-blot coupled to immunostaining confirmed the expression of S1P1 in ASC-differentiated to endothelial-like cells.

    Article Snippet: Primary antibody anti-PI3K (Abcam), anti-S1P1 receptor (Cayman Chemical Company) or anti-β-actin (Sigma) was incubated at 4°C overnight or 2 hours at room temperature then followed by 1-h incubation with corresponding secondary antibodies from Molecular Probe.

    Techniques: Expressing, Western Blot, Immunostaining, Fluorescence

    Immunoblotting of S1PR expression in control and colitic colon segments. Representative images of total membrane preps (50 μg protein) from control and colitic colon tissues, from 8–12 independent rats, that were probed with S1PR1 (a), S1PR2 (b) and S1PR3 (c) antibodies. Band intensities were normalized to actin and expressed relative to the controls. Data is mean ± SEM, * P

    Journal: PLoS ONE

    Article Title: The effect of sphingosine-1-phosphate on colonic smooth muscle contractility: Modulation by TNBS-induced colitis

    doi: 10.1371/journal.pone.0170792

    Figure Lengend Snippet: Immunoblotting of S1PR expression in control and colitic colon segments. Representative images of total membrane preps (50 μg protein) from control and colitic colon tissues, from 8–12 independent rats, that were probed with S1PR1 (a), S1PR2 (b) and S1PR3 (c) antibodies. Band intensities were normalized to actin and expressed relative to the controls. Data is mean ± SEM, * P

    Article Snippet: The membranes were immunoblotted with rabbit anti-S1PR1 (Cayman Chemical, MI, USA), rabbit anti-S1PR2 (Sigma Aldrich, Munich, Germany), rabbit anti-S1PR3 (Cayman Chemicals, MI, USA) or rabbit anti-actin (Abcam, MA, USA).

    Techniques: Expressing

    Proposed mechanism of S1P induced contraction in control and colitic colon. In control colon S1P activates S1PR1 and S1PR2 and induce contractions that are dependent on calcium influx via L-type calcium channels, calcium release from the sarcoplasmic reticulum and calcium sensitization pathways including PKC and Rho kinase (A). In colitic colon however S1P induces larger contractions via S1PR2 that are independent of calcium influx through L-type calcium channels yet it involves intracellular calcium release and calcium sensitization pathways (B). The contribution of these pathways however appears to be different in control and inflamed colon segments. Whereas the blockade of a single pathway completely abolishes the response to S1P in control segments, S1P induced contractions appear to be salvaged by other pathways in inflamed colon segments. Eradication of S1P induced contraction in inflamed colon require simultaneous blockade of intracellular calcium release (SERCA) and calcium sensitization pathways (PKC and Rho kinase).

    Journal: PLoS ONE

    Article Title: The effect of sphingosine-1-phosphate on colonic smooth muscle contractility: Modulation by TNBS-induced colitis

    doi: 10.1371/journal.pone.0170792

    Figure Lengend Snippet: Proposed mechanism of S1P induced contraction in control and colitic colon. In control colon S1P activates S1PR1 and S1PR2 and induce contractions that are dependent on calcium influx via L-type calcium channels, calcium release from the sarcoplasmic reticulum and calcium sensitization pathways including PKC and Rho kinase (A). In colitic colon however S1P induces larger contractions via S1PR2 that are independent of calcium influx through L-type calcium channels yet it involves intracellular calcium release and calcium sensitization pathways (B). The contribution of these pathways however appears to be different in control and inflamed colon segments. Whereas the blockade of a single pathway completely abolishes the response to S1P in control segments, S1P induced contractions appear to be salvaged by other pathways in inflamed colon segments. Eradication of S1P induced contraction in inflamed colon require simultaneous blockade of intracellular calcium release (SERCA) and calcium sensitization pathways (PKC and Rho kinase).

    Article Snippet: The membranes were immunoblotted with rabbit anti-S1PR1 (Cayman Chemical, MI, USA), rabbit anti-S1PR2 (Sigma Aldrich, Munich, Germany), rabbit anti-S1PR3 (Cayman Chemicals, MI, USA) or rabbit anti-actin (Abcam, MA, USA).

    Techniques:

    The Role of S1PR in S1P induced contraction in control and colitic colon. Colonic segments from control (A) and colitic (B) rats were equilibrated in Krebs solution for 30 min and contracted twice with KCL (80mM) with consecutive washes after the maximal contraction was reached. Tissues were then preincubated with S1PR1 agonist (SEW2871, 1μM) S1PR1 antagonist (W146, 10μM), S1PR2 antagonist (JTE-013, 1μM) or with S1PR3 antagonist (CAY-10444, 10μM) for 30 min before the addition of S1P (40μM). Data is mean ± SEM of S1P induced contraction is expressed relative to the second KCl-induced contraction, * P

    Journal: PLoS ONE

    Article Title: The effect of sphingosine-1-phosphate on colonic smooth muscle contractility: Modulation by TNBS-induced colitis

    doi: 10.1371/journal.pone.0170792

    Figure Lengend Snippet: The Role of S1PR in S1P induced contraction in control and colitic colon. Colonic segments from control (A) and colitic (B) rats were equilibrated in Krebs solution for 30 min and contracted twice with KCL (80mM) with consecutive washes after the maximal contraction was reached. Tissues were then preincubated with S1PR1 agonist (SEW2871, 1μM) S1PR1 antagonist (W146, 10μM), S1PR2 antagonist (JTE-013, 1μM) or with S1PR3 antagonist (CAY-10444, 10μM) for 30 min before the addition of S1P (40μM). Data is mean ± SEM of S1P induced contraction is expressed relative to the second KCl-induced contraction, * P

    Article Snippet: The membranes were immunoblotted with rabbit anti-S1PR1 (Cayman Chemical, MI, USA), rabbit anti-S1PR2 (Sigma Aldrich, Munich, Germany), rabbit anti-S1PR3 (Cayman Chemicals, MI, USA) or rabbit anti-actin (Abcam, MA, USA).

    Techniques:

    S1P1 receptor expression in human ovarian carcinomas . (A) Normal ovary showing diffusely stained S1P+ stromal cells (white arrows) and a blood vessel (BV) with intensely stained S1P1+ endothelial cells (black arrow) (200×). (B) Serous ovarian tumor with S1P1+ stroma (white arrows) and unstained surface epithelium (200×). (C) blood vessels in normal ovary (BV, arrows) are S1P1 + (600×). (D) S1P1+ endometrioid ovarian tumor with patches of S1P1+staining within the surrounding stroma (white arrows) adjacent to unstained endometrioid tumor (400×). (A-D) are paraffin-embedded sections.

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: S1P1 receptor expression in human ovarian carcinomas . (A) Normal ovary showing diffusely stained S1P+ stromal cells (white arrows) and a blood vessel (BV) with intensely stained S1P1+ endothelial cells (black arrow) (200×). (B) Serous ovarian tumor with S1P1+ stroma (white arrows) and unstained surface epithelium (200×). (C) blood vessels in normal ovary (BV, arrows) are S1P1 + (600×). (D) S1P1+ endometrioid ovarian tumor with patches of S1P1+staining within the surrounding stroma (white arrows) adjacent to unstained endometrioid tumor (400×). (A-D) are paraffin-embedded sections.

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Staining

    In ovarian tumors S1P1 expression was observed near T and B cells . Alternate serial sections of a serous ovarian tumor of the hen showing ( A) Bu1a+ cells in the stroma (arrows) and diffuse tissue stain (open arrow), ( B) CD4 T cells around tumor glands (arrows), ( C) S1P1 expression on the epithelium of tumor glands (arrow) and ( D) CD8 T cells (arrows) in the stroma (original magnification 100×).

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: In ovarian tumors S1P1 expression was observed near T and B cells . Alternate serial sections of a serous ovarian tumor of the hen showing ( A) Bu1a+ cells in the stroma (arrows) and diffuse tissue stain (open arrow), ( B) CD4 T cells around tumor glands (arrows), ( C) S1P1 expression on the epithelium of tumor glands (arrow) and ( D) CD8 T cells (arrows) in the stroma (original magnification 100×).

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Staining

    S1P1 protein expression in hen and human tissue . S1P1 immunoreactions are similar in hen and human ovaries and ovarian tumors. Three bands at 47, 72, 108 kDa were observed. The band at 47 kDa was faint, while bands at 72 and 108 kDa were consistently present in all tissues but vary in intensity. The 47 kD band was not significantly enhanced using a membrane enriched (18,000 × g pellet) fraction. The pattern of immunoreactive bands was identical in the positive control recommended by the manufacturer (rat brain) and in hen brain and spleen. The bands were absent in control incubations in which the primary antibody was pre-adsorbed with a blocking peptide or in which the primary antibody was omitted.

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: S1P1 protein expression in hen and human tissue . S1P1 immunoreactions are similar in hen and human ovaries and ovarian tumors. Three bands at 47, 72, 108 kDa were observed. The band at 47 kDa was faint, while bands at 72 and 108 kDa were consistently present in all tissues but vary in intensity. The 47 kD band was not significantly enhanced using a membrane enriched (18,000 × g pellet) fraction. The pattern of immunoreactive bands was identical in the positive control recommended by the manufacturer (rat brain) and in hen brain and spleen. The bands were absent in control incubations in which the primary antibody was pre-adsorbed with a blocking peptide or in which the primary antibody was omitted.

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Positive Control, Blocking Assay

    Localization of S1P1 receptor protein expression in normal hen ovary . ( A ) S1P1+ cells (black arrows) in theca of a mature follicle (F) and within small blood vessels (BV; white arrows) in ovarian stroma (100×). Primordial follicles (f) have comparatively little S1P1+ expression. ( B ) Endothelial cells of blood vessels (BV) in the theca externa (TE) of a follicle (F) and ovarian stroma are S1P1+ (200×). ( C ) Surface epithelial cells (EpC) showing intense S1P1+ expression (400×). ( D ) An atretic follicle (af) with characteristic infiltrating S1P1+ immune cells (100×). Inset: High magnification of (D) showing an S1P1+ immune cell (1000×). ( E ) Well developed blood vessels (BV) in the medullary region of the ovary also contain S1P1+ endothelial cells (400×) but staining is lighter and more diffuse than in stromal blood vessels seen in (A) and (B). Inset: high magnification (800×) shows detail of smooth muscle cells (SmC) and endothelial cells (EnC). ( A-C ) are frozen tissues; ( D ) and ( E ) are paraffin-embedded.

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: Localization of S1P1 receptor protein expression in normal hen ovary . ( A ) S1P1+ cells (black arrows) in theca of a mature follicle (F) and within small blood vessels (BV; white arrows) in ovarian stroma (100×). Primordial follicles (f) have comparatively little S1P1+ expression. ( B ) Endothelial cells of blood vessels (BV) in the theca externa (TE) of a follicle (F) and ovarian stroma are S1P1+ (200×). ( C ) Surface epithelial cells (EpC) showing intense S1P1+ expression (400×). ( D ) An atretic follicle (af) with characteristic infiltrating S1P1+ immune cells (100×). Inset: High magnification of (D) showing an S1P1+ immune cell (1000×). ( E ) Well developed blood vessels (BV) in the medullary region of the ovary also contain S1P1+ endothelial cells (400×) but staining is lighter and more diffuse than in stromal blood vessels seen in (A) and (B). Inset: high magnification (800×) shows detail of smooth muscle cells (SmC) and endothelial cells (EnC). ( A-C ) are frozen tissues; ( D ) and ( E ) are paraffin-embedded.

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Staining

    Localization of S1P1 receptor protein expression in hen ovarian tumors . ( A ) An example of a mucinous ovarian tumor with S1P1+ staining associated with surface epithelium (black arrow) and mucin-secreting structures (black arrow) (100×). ( B ) H E stained section (100×) serial to that in (A) shows mucinous histology. ( C ) Higher magnification of (A) showing S1P1+ mucin-secreting glandular (MG) structures (black arrows) (400×). ( D ) H E stained section of a serous ovarian tumor (100×). ( E ) Serial section showing minimal stromal cell stain for S1P1+ but intense surface epithelial cell staining (black arrow) and lighter more diffuse S1P1+ sub-epithelial cells (white arrow) (100×). ( F ) High magnification (of box) showing S1P1+ surface epithelial cells (EpC) (600×). ( G ) Clear-cell ovarian tumor (T; left of dotted red line) with negligible S1P1+ in tumor and S1P1+ cells in adjacent uninvolved stroma (100×). ( H ) H E-stained serial section from the same tumor region in (G) shows cellular detail of clear cell carcinoma (400×). ( I ) Higher magnification of box in(G) showing stromal blood vessels (BV) with S1P1+ endothelial cells and stromal cells (S) (200×). ( J ) H E stained section of late stage endometrioid tumor (100×). ( K ) S1P1+ is highly expressed in cells in the tumor periphery and to a lesser extent in tumor stroma (100×). ( L ) High magnification of box in (K) showing cytoplasmic staining of endometrioid tumor cells (400x). All images are from paraffin-embedded tissue, except clear cell carcinoma (G-I) which is a frozen section.

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: Localization of S1P1 receptor protein expression in hen ovarian tumors . ( A ) An example of a mucinous ovarian tumor with S1P1+ staining associated with surface epithelium (black arrow) and mucin-secreting structures (black arrow) (100×). ( B ) H E stained section (100×) serial to that in (A) shows mucinous histology. ( C ) Higher magnification of (A) showing S1P1+ mucin-secreting glandular (MG) structures (black arrows) (400×). ( D ) H E stained section of a serous ovarian tumor (100×). ( E ) Serial section showing minimal stromal cell stain for S1P1+ but intense surface epithelial cell staining (black arrow) and lighter more diffuse S1P1+ sub-epithelial cells (white arrow) (100×). ( F ) High magnification (of box) showing S1P1+ surface epithelial cells (EpC) (600×). ( G ) Clear-cell ovarian tumor (T; left of dotted red line) with negligible S1P1+ in tumor and S1P1+ cells in adjacent uninvolved stroma (100×). ( H ) H E-stained serial section from the same tumor region in (G) shows cellular detail of clear cell carcinoma (400×). ( I ) Higher magnification of box in(G) showing stromal blood vessels (BV) with S1P1+ endothelial cells and stromal cells (S) (200×). ( J ) H E stained section of late stage endometrioid tumor (100×). ( K ) S1P1+ is highly expressed in cells in the tumor periphery and to a lesser extent in tumor stroma (100×). ( L ) High magnification of box in (K) showing cytoplasmic staining of endometrioid tumor cells (400x). All images are from paraffin-embedded tissue, except clear cell carcinoma (G-I) which is a frozen section.

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Staining

    In normal hen ovary S1P1 expression was observed in areas of immune cell infiltration . ( Row A) Immune cells (Bu1a+ and CD3+) are adjacent to the follicle (f) in the ovarian stroma near a transverse blood vessel (arrow in column S1P1). Cells lining the vessel near the follicle (f) are S1P1+ (row A, S1P1) (original magnification 100×). ( Row B) High magnification (see red boxes in row A) showing B and T cells clustered within the stroma near S1P1 stained vascular endothelium (arrow). Some immune cells are also S1P1+ (open arrow) (original magnification 400×). ( Row C) Cross-section of a large blood vessel shows Bu1a and CD3 positive cells are clustered near the blood vessel. The apical surfaces of endothelial cell express S1P1 (original magnification 100×).

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: In normal hen ovary S1P1 expression was observed in areas of immune cell infiltration . ( Row A) Immune cells (Bu1a+ and CD3+) are adjacent to the follicle (f) in the ovarian stroma near a transverse blood vessel (arrow in column S1P1). Cells lining the vessel near the follicle (f) are S1P1+ (row A, S1P1) (original magnification 100×). ( Row B) High magnification (see red boxes in row A) showing B and T cells clustered within the stroma near S1P1 stained vascular endothelium (arrow). Some immune cells are also S1P1+ (open arrow) (original magnification 400×). ( Row C) Cross-section of a large blood vessel shows Bu1a and CD3 positive cells are clustered near the blood vessel. The apical surfaces of endothelial cell express S1P1 (original magnification 100×).

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Staining

    S1P1 mRNA expression in hen tissues . ( A ) S1P1 mRNA (226 bp) is expressed in both normal and tumor ovaries. Examples of mRNA in tumors with endometrioid (En), serous (Sr), and mucinous (Mc) histology are shown. ( B ) Examples of other hen tissues that express S1P1 mRNA (226 bp) include liver (LVR), kidney (KDNY), skeletal muscle (MSCL), oviduct (OVDT) and spleen. Normal ovary and spleen are from the same hens (1-4). β-actin (300 bp) was used as a loading control. Controls for S1P1 primer include the positive control (+) lane which was the sample from an earlier experiment used to verify the RNA sequence. The negative control lane omitted the cDNA.

    Journal: Journal of Ovarian Research

    Article Title: Sphingosine-1 phosphate receptor (S1p1), a critical receptor controlling human lymphocyte trafficking, is expressed in hen and human ovaries and ovarian tumors

    doi: 10.1186/1757-2215-4-4

    Figure Lengend Snippet: S1P1 mRNA expression in hen tissues . ( A ) S1P1 mRNA (226 bp) is expressed in both normal and tumor ovaries. Examples of mRNA in tumors with endometrioid (En), serous (Sr), and mucinous (Mc) histology are shown. ( B ) Examples of other hen tissues that express S1P1 mRNA (226 bp) include liver (LVR), kidney (KDNY), skeletal muscle (MSCL), oviduct (OVDT) and spleen. Normal ovary and spleen are from the same hens (1-4). β-actin (300 bp) was used as a loading control. Controls for S1P1 primer include the positive control (+) lane which was the sample from an earlier experiment used to verify the RNA sequence. The negative control lane omitted the cDNA.

    Article Snippet: As a control for antibody specificity the anti-S1P1 antibody was pre-absorbed with blocking peptide (Cayman, Ann Arbor, MI) (1:1, v/v; 45 minutes, 22°C).

    Techniques: Expressing, Positive Control, Sequencing, Negative Control