anti s1p1  (Alomone Labs)


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    Alomone Labs anti s1p1
    A model for the intervention of AD2900 in the localization of murine T lymphocytes As an antagonist to S1P receptors 1–5, AD2900 can compete with S1P to bind S1P receptors leading to reduced S1P signaling and enhanced expression of <t>S1P1</t> on T cells in S1P-rich environments such as the blood and the spleen. This altered expression, together with decreased CCR7 expression, inhibits T-cell entry into the lymph nodes (LNs) from the blood, causing accumulation of T cells in the blood. However, the entry of T cells to the spleen is not affected because it is not S1P dependent. Since Tcm-like cells express CCR7, these cells are attracted to the spleen and accumulate in it; yet, S1P1 elevated expression may have an effect on the S1P-dependent ingression of these cells from the MZ to the white pulp. Tef/em-like cells, which are CCR7 negative, are the primary T-cell subpopulation in the blood after AD2900 treatment. The significant decrease in naive T-cell counts in the circulation and peripheral lymphoid tissues tested may be explained by the inhibition of S1P signaling in the thymus leading to attenuated T-cell egression from the thymus to the circulation. Arrow key: thick = response; dashed = inhibition.
    Anti S1p1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti s1p1/product/Alomone Labs
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti s1p1 - by Bioz Stars, 2022-11
    88/100 stars

    Images

    1) Product Images from "The novel sphingosine-1-phosphate receptors antagonist AD2900 affects lymphocyte activation and inhibits T-cell entry into the lymph nodes"

    Article Title: The novel sphingosine-1-phosphate receptors antagonist AD2900 affects lymphocyte activation and inhibits T-cell entry into the lymph nodes

    Journal: Oncotarget

    doi: 10.18632/oncotarget.18626

    A model for the intervention of AD2900 in the localization of murine T lymphocytes As an antagonist to S1P receptors 1–5, AD2900 can compete with S1P to bind S1P receptors leading to reduced S1P signaling and enhanced expression of S1P1 on T cells in S1P-rich environments such as the blood and the spleen. This altered expression, together with decreased CCR7 expression, inhibits T-cell entry into the lymph nodes (LNs) from the blood, causing accumulation of T cells in the blood. However, the entry of T cells to the spleen is not affected because it is not S1P dependent. Since Tcm-like cells express CCR7, these cells are attracted to the spleen and accumulate in it; yet, S1P1 elevated expression may have an effect on the S1P-dependent ingression of these cells from the MZ to the white pulp. Tef/em-like cells, which are CCR7 negative, are the primary T-cell subpopulation in the blood after AD2900 treatment. The significant decrease in naive T-cell counts in the circulation and peripheral lymphoid tissues tested may be explained by the inhibition of S1P signaling in the thymus leading to attenuated T-cell egression from the thymus to the circulation. Arrow key: thick = response; dashed = inhibition.
    Figure Legend Snippet: A model for the intervention of AD2900 in the localization of murine T lymphocytes As an antagonist to S1P receptors 1–5, AD2900 can compete with S1P to bind S1P receptors leading to reduced S1P signaling and enhanced expression of S1P1 on T cells in S1P-rich environments such as the blood and the spleen. This altered expression, together with decreased CCR7 expression, inhibits T-cell entry into the lymph nodes (LNs) from the blood, causing accumulation of T cells in the blood. However, the entry of T cells to the spleen is not affected because it is not S1P dependent. Since Tcm-like cells express CCR7, these cells are attracted to the spleen and accumulate in it; yet, S1P1 elevated expression may have an effect on the S1P-dependent ingression of these cells from the MZ to the white pulp. Tef/em-like cells, which are CCR7 negative, are the primary T-cell subpopulation in the blood after AD2900 treatment. The significant decrease in naive T-cell counts in the circulation and peripheral lymphoid tissues tested may be explained by the inhibition of S1P signaling in the thymus leading to attenuated T-cell egression from the thymus to the circulation. Arrow key: thick = response; dashed = inhibition.

    Techniques Used: Expressing, Inhibition

    The influence of AD2900 on S1P1- and CCR7-positive T-cell populations in blood, spleen, and peripheral lymph nodes C57BL/6 mice were orally administered with 1.8, 2.7, and 3.6 mg/l AD2900 or 1.8 mg/l FTY720 for 2 days, as shown in Figure 4 . Leukocytes from blood, spleen, and pLNs were collected and stained with CD3e and S1P1 or CCR7 fluorescent antibodies and then analyzed by FACS analysis. The percentages of S1P1+ CD3e+ T cells from blood (A) , spleen (B) , and pLNs (C) are shown. The percentages of CCR7+ CD3e+ T cells from blood (D) , spleen (E) , and pLNs (F) are shown. All the significances are compared to untreated healthy mice. Results summarize at least three independent experiments. Results of Student’s t -test:*(P
    Figure Legend Snippet: The influence of AD2900 on S1P1- and CCR7-positive T-cell populations in blood, spleen, and peripheral lymph nodes C57BL/6 mice were orally administered with 1.8, 2.7, and 3.6 mg/l AD2900 or 1.8 mg/l FTY720 for 2 days, as shown in Figure 4 . Leukocytes from blood, spleen, and pLNs were collected and stained with CD3e and S1P1 or CCR7 fluorescent antibodies and then analyzed by FACS analysis. The percentages of S1P1+ CD3e+ T cells from blood (A) , spleen (B) , and pLNs (C) are shown. The percentages of CCR7+ CD3e+ T cells from blood (D) , spleen (E) , and pLNs (F) are shown. All the significances are compared to untreated healthy mice. Results summarize at least three independent experiments. Results of Student’s t -test:*(P

    Techniques Used: Mouse Assay, Staining, FACS

    AD2900 downregulates the percentage of S1P1- and CCR7-expressing cells in PBMCs (A, B) The percentages of S1P1-positive PBMCs after the treatment with different concentrations of AD2900, FTY720, or SEW2871 and at different time points were examined by FACS analysis. S1P1 expression was tested in PBMCs after a 30-min treatment with AD2900 at different concentrations (A) or after a 30-min or 60-min treatment with 100 nM AD2900, FTY720, or SEW2871 (B) . (C) The percentage of CCR7-positive PBMCs was tested by FACS analysis after a 30-min treatment with 100 nM AD2900, FTY720, or SEW2871. All the significances are compared to untreated PBMCs. Results summarize the results of at least four independent experiments. Results of Student’s t -test: *(P
    Figure Legend Snippet: AD2900 downregulates the percentage of S1P1- and CCR7-expressing cells in PBMCs (A, B) The percentages of S1P1-positive PBMCs after the treatment with different concentrations of AD2900, FTY720, or SEW2871 and at different time points were examined by FACS analysis. S1P1 expression was tested in PBMCs after a 30-min treatment with AD2900 at different concentrations (A) or after a 30-min or 60-min treatment with 100 nM AD2900, FTY720, or SEW2871 (B) . (C) The percentage of CCR7-positive PBMCs was tested by FACS analysis after a 30-min treatment with 100 nM AD2900, FTY720, or SEW2871. All the significances are compared to untreated PBMCs. Results summarize the results of at least four independent experiments. Results of Student’s t -test: *(P

    Techniques Used: Expressing, FACS

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    • anti s1pr1 antibody  (Alomone Labs)
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    Alomone Labs anti s1pr1 antibody
    Representative images of [ 3 H]CS1P1 autoradiograph, <t>S1PR1</t> immunostaining, and Hematoxylin and eosin (H E) staining in postmortem human DLPFC tissues. The distribution of [ 3 H]CS1P1 matched well with anti-S1PR1 antibody, and was mainly located in the gray matter regions as indicated in the H E staining.
    Anti S1pr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti s1pr3 edg3 extracellular antibody  (Alomone Labs)
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    Alomone Labs anti s1pr3 edg3 extracellular antibody
    Representative images of [ 3 H]CS1P1 autoradiograph, <t>S1PR1</t> immunostaining, and Hematoxylin and eosin (H E) staining in postmortem human DLPFC tissues. The distribution of [ 3 H]CS1P1 matched well with anti-S1PR1 antibody, and was mainly located in the gray matter regions as indicated in the H E staining.
    Anti S1pr3 Edg3 Extracellular Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative images of [ 3 H]CS1P1 autoradiograph, S1PR1 immunostaining, and Hematoxylin and eosin (H E) staining in postmortem human DLPFC tissues. The distribution of [ 3 H]CS1P1 matched well with anti-S1PR1 antibody, and was mainly located in the gray matter regions as indicated in the H E staining.

    Journal: Frontiers in Psychiatry

    Article Title: Differential Sphingosine-1-Phosphate Receptor-1 Protein Expression in the Dorsolateral Prefrontal Cortex Between Schizophrenia Type 1 and Type 2

    doi: 10.3389/fpsyt.2022.827981

    Figure Lengend Snippet: Representative images of [ 3 H]CS1P1 autoradiograph, S1PR1 immunostaining, and Hematoxylin and eosin (H E) staining in postmortem human DLPFC tissues. The distribution of [ 3 H]CS1P1 matched well with anti-S1PR1 antibody, and was mainly located in the gray matter regions as indicated in the H E staining.

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 h at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Autoradiography, Immunostaining, Staining

    ARG S1PR1 intensity expression (in fmol/mg) comparison between normal controls, schizophrenia Type 1, and schizophrenia Type 2 (* p

    Journal: Frontiers in Psychiatry

    Article Title: Differential Sphingosine-1-Phosphate Receptor-1 Protein Expression in the Dorsolateral Prefrontal Cortex Between Schizophrenia Type 1 and Type 2

    doi: 10.3389/fpsyt.2022.827981

    Figure Lengend Snippet: ARG S1PR1 intensity expression (in fmol/mg) comparison between normal controls, schizophrenia Type 1, and schizophrenia Type 2 (* p

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 h at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Expressing

    Immunohistochemistry (IHC) of S1PR1 in postmortem DLPFC tissues from the representative normal control and schizophrenia Type 1 and Type 2.

    Journal: Frontiers in Psychiatry

    Article Title: Differential Sphingosine-1-Phosphate Receptor-1 Protein Expression in the Dorsolateral Prefrontal Cortex Between Schizophrenia Type 1 and Type 2

    doi: 10.3389/fpsyt.2022.827981

    Figure Lengend Snippet: Immunohistochemistry (IHC) of S1PR1 in postmortem DLPFC tissues from the representative normal control and schizophrenia Type 1 and Type 2.

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 h at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Immunohistochemistry

    Autoradiography images of S1PR1 using [ 3 H]CS1P1 in postmortem DLPFC tissues from representative normal control, schizophrenia Type 1, and schizophrenia Type 2. In general, [ 3 H]CS1P1 was higher in Type 2 schizophrenia subjects compared with normal control and Type 1 schizophrenia subjects.

    Journal: Frontiers in Psychiatry

    Article Title: Differential Sphingosine-1-Phosphate Receptor-1 Protein Expression in the Dorsolateral Prefrontal Cortex Between Schizophrenia Type 1 and Type 2

    doi: 10.3389/fpsyt.2022.827981

    Figure Lengend Snippet: Autoradiography images of S1PR1 using [ 3 H]CS1P1 in postmortem DLPFC tissues from representative normal control, schizophrenia Type 1, and schizophrenia Type 2. In general, [ 3 H]CS1P1 was higher in Type 2 schizophrenia subjects compared with normal control and Type 1 schizophrenia subjects.

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 h at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Autoradiography

    ARG S1PR1 intensity expression (fmol/mg) triplicate measures (M1, M2, and M3) in the DLPFC from normal controls, schizophrenia Type 1, and schizophrenia Type 2.

    Journal: Frontiers in Psychiatry

    Article Title: Differential Sphingosine-1-Phosphate Receptor-1 Protein Expression in the Dorsolateral Prefrontal Cortex Between Schizophrenia Type 1 and Type 2

    doi: 10.3389/fpsyt.2022.827981

    Figure Lengend Snippet: ARG S1PR1 intensity expression (fmol/mg) triplicate measures (M1, M2, and M3) in the DLPFC from normal controls, schizophrenia Type 1, and schizophrenia Type 2.

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 h at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Expressing

    Immunohistochemistry analysis of S1PR1 in hind limb muscle of sham and S aureus -infected mice. S1PR1 was significantly upregulated in the muscle of infected mice (red arrow) comparing with sham mice (green arrow), scale bar = 100 μ m.

    Journal: Molecular Imaging

    Article Title: PET Study of Sphingosine-1-phosphate Receptor 1 Expression in Response to S. aureus Infection

    doi: 10.1155/2021/9982020

    Figure Lengend Snippet: Immunohistochemistry analysis of S1PR1 in hind limb muscle of sham and S aureus -infected mice. S1PR1 was significantly upregulated in the muscle of infected mice (red arrow) comparing with sham mice (green arrow), scale bar = 100 μ m.

    Article Snippet: After washing in PBS, all sections were then incubated with rabbit anti-S1PR1 (Alomone, Israel) antibody overnight at 4°C and then washed and incubated with ImmPRESS HRP Horse anti-rabbit polymer for 1 hour at room temperature and developed with ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Immunohistochemistry, Infection, Mouse Assay

    MicroPET imaging of S1PR1 activity in S aureus -infected mice. (a) Radiosynthesis of S1PR1-specific radiotracer, [ 18 F]TZ4877; (b) representative sagittal microPET images of [ 18 F]TZ4877 in mice. Comparing with sham mice, the tracer uptake was significantly higher in the infected mice, and the increased uptake of the tracer showed S aureus dose dependent; (c) the tracer uptake in the brain was quantified; time-activity curves showed that the tracer uptake in infected mice was significantly higher than mice without infections; (d) the average tracer uptake in the brain from 30 to 50 min of the PET scan showed a dose-dependent manner. Data represent the mean ± SEM, n = 3 for each group.

    Journal: Molecular Imaging

    Article Title: PET Study of Sphingosine-1-phosphate Receptor 1 Expression in Response to S. aureus Infection

    doi: 10.1155/2021/9982020

    Figure Lengend Snippet: MicroPET imaging of S1PR1 activity in S aureus -infected mice. (a) Radiosynthesis of S1PR1-specific radiotracer, [ 18 F]TZ4877; (b) representative sagittal microPET images of [ 18 F]TZ4877 in mice. Comparing with sham mice, the tracer uptake was significantly higher in the infected mice, and the increased uptake of the tracer showed S aureus dose dependent; (c) the tracer uptake in the brain was quantified; time-activity curves showed that the tracer uptake in infected mice was significantly higher than mice without infections; (d) the average tracer uptake in the brain from 30 to 50 min of the PET scan showed a dose-dependent manner. Data represent the mean ± SEM, n = 3 for each group.

    Article Snippet: After washing in PBS, all sections were then incubated with rabbit anti-S1PR1 (Alomone, Israel) antibody overnight at 4°C and then washed and incubated with ImmPRESS HRP Horse anti-rabbit polymer for 1 hour at room temperature and developed with ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Imaging, Activity Assay, Infection, Mouse Assay, Positron Emission Tomography

    MicroPET imaging of S1PR1 activity in S aureus -infected mice. (a) Representative sagittal microPET images of [ 18 F]TZ4877 in the hind limb of mice. The tracer uptake was relatively low in the hind limb muscle with a SUV of ~1.5 in sham mice. Comparing with sham mice, the tracer uptake was significantly higher in the hind limb of infected mice; (b) time-activity curves showed that the tracer uptake in infected mice was significantly higher than sham mice; (c) the average tracer uptake in the hind limb muscle from 30 to 50 min of the PET scan showed a ~39% increase of SUV in infected mice with a P value of 0.0082. Data represent the mean ± SEM, n = 3 for each group.

    Journal: Molecular Imaging

    Article Title: PET Study of Sphingosine-1-phosphate Receptor 1 Expression in Response to S. aureus Infection

    doi: 10.1155/2021/9982020

    Figure Lengend Snippet: MicroPET imaging of S1PR1 activity in S aureus -infected mice. (a) Representative sagittal microPET images of [ 18 F]TZ4877 in the hind limb of mice. The tracer uptake was relatively low in the hind limb muscle with a SUV of ~1.5 in sham mice. Comparing with sham mice, the tracer uptake was significantly higher in the hind limb of infected mice; (b) time-activity curves showed that the tracer uptake in infected mice was significantly higher than sham mice; (c) the average tracer uptake in the hind limb muscle from 30 to 50 min of the PET scan showed a ~39% increase of SUV in infected mice with a P value of 0.0082. Data represent the mean ± SEM, n = 3 for each group.

    Article Snippet: After washing in PBS, all sections were then incubated with rabbit anti-S1PR1 (Alomone, Israel) antibody overnight at 4°C and then washed and incubated with ImmPRESS HRP Horse anti-rabbit polymer for 1 hour at room temperature and developed with ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Imaging, Activity Assay, Infection, Mouse Assay, Positron Emission Tomography

    Autoradiograph analysis of S1PR1 using S1PR1 specific [ 3 H]CS1P1 in control and schizophrenia DLPFC. A) Comparison among control, schizophrenia Type 1 and schizophrenia Type 2 (* represents p

    Journal: bioRxiv

    Article Title: Differential sphingosine-1-phosphate receptor-1 (S1PR1) protein expressions in the dorsolateral prefrontal cortex between schizophrenia Type 1 and Type 2

    doi: 10.1101/2021.05.15.444302

    Figure Lengend Snippet: Autoradiograph analysis of S1PR1 using S1PR1 specific [ 3 H]CS1P1 in control and schizophrenia DLPFC. A) Comparison among control, schizophrenia Type 1 and schizophrenia Type 2 (* represents p

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 hour at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Autoradiography

    Representative images of autoradiograph analysis of S1PR1 using S1PR1 specific [ 3 H]CS1P1 in control and schizophrenia DLPFC.

    Journal: bioRxiv

    Article Title: Differential sphingosine-1-phosphate receptor-1 (S1PR1) protein expressions in the dorsolateral prefrontal cortex between schizophrenia Type 1 and Type 2

    doi: 10.1101/2021.05.15.444302

    Figure Lengend Snippet: Representative images of autoradiograph analysis of S1PR1 using S1PR1 specific [ 3 H]CS1P1 in control and schizophrenia DLPFC.

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 hour at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Autoradiography

    Immunohistochemistry of S1PR1 in control and schizophrenia DLPFC.

    Journal: bioRxiv

    Article Title: Differential sphingosine-1-phosphate receptor-1 (S1PR1) protein expressions in the dorsolateral prefrontal cortex between schizophrenia Type 1 and Type 2

    doi: 10.1101/2021.05.15.444302

    Figure Lengend Snippet: Immunohistochemistry of S1PR1 in control and schizophrenia DLPFC.

    Article Snippet: After that, all sections were stained with anti-S1PR1 antibody (Alomone, Jerusalem, Israel) overnight at 4°C, washed and followed by incubation with ImmPRESS HRP Horse anti-rabbit polymer for 1 hour at RT, and developed using ImmPACT DAB (Vector Laboratories, Burlingame, CA).

    Techniques: Immunohistochemistry