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Santa Cruz Biotechnology anti rxrα d 20
Anti Rxrα D 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rxrα d 20/product/Santa Cruz Biotechnology
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
anti rxrα d 20 - by Bioz Stars, 2020-05
85/100 stars

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Immunoprecipitation:

Article Title: SP1 and RARα regulate AGAP2 expression in cancer
Article Snippet: .. Afterwards, crosslinked proteins of interest were immunoprecipitated with 2 μg of either anti-RARα (C-20), anti-RXRα (D-20) (both from Santa Cruz Biotechnology), anti-PCAF (C14G9, Cell Signaling), or 1 μg of anti-SP1 (D4C3, Cell Signalling). .. Anti-Pol II (N-20, Santa Cruz Biotechnology) was used as positive control and rabbit IgG (Invitrogen) as negative control.

Generated:

Article Title: Transactivation of a DR-1 PPRE by a human constitutive androstane receptor variant expressed from internal protein translation start sites
Article Snippet: .. One-hundred and fifty micrograms of total protein were dissolved in loading buffer and were resolved by SDS-PAGE and separated proteins were transferred to PVDF membrane (BioRad, Hercules, CA), then probed with primary antibodies against human CAR (generated in our laboratory) or RXRα (D-20) (Santa Cruz). .. Secondary antibody goat–anti-rabbit-IgG-HRP (Santa Cruz Biotechnology, Santa Cruz, CA) was used at 1:5000 dilution.

SDS Page:

Article Title: Transactivation of a DR-1 PPRE by a human constitutive androstane receptor variant expressed from internal protein translation start sites
Article Snippet: .. One-hundred and fifty micrograms of total protein were dissolved in loading buffer and were resolved by SDS-PAGE and separated proteins were transferred to PVDF membrane (BioRad, Hercules, CA), then probed with primary antibodies against human CAR (generated in our laboratory) or RXRα (D-20) (Santa Cruz). .. Secondary antibody goat–anti-rabbit-IgG-HRP (Santa Cruz Biotechnology, Santa Cruz, CA) was used at 1:5000 dilution.

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    Santa Cruz Biotechnology anti rxrα
    Transcription of Ccl6 and Ccl9 is regulated by <t>RXRα</t> via DR-1 rexinoid response elements. ( A and B ) RAW 264.7 macrophages were cotransfected with a luciferase reporter plasmid under the transcriptional control of either the Ccl6 (pGL3-CCL6-luc)
    Anti Rxrα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rxrα/product/Santa Cruz Biotechnology
    Average 89 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    anti rxrα - by Bioz Stars, 2020-05
    89/100 stars
      Buy from Supplier

    85
    Santa Cruz Biotechnology chip quality anti rxrα antibody
    Correlation of gender differential <t>RXRα,</t> Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).
    Chip Quality Anti Rxrα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip quality anti rxrα antibody/product/Santa Cruz Biotechnology
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    chip quality anti rxrα antibody - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

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    Transcription of Ccl6 and Ccl9 is regulated by RXRα via DR-1 rexinoid response elements. ( A and B ) RAW 264.7 macrophages were cotransfected with a luciferase reporter plasmid under the transcriptional control of either the Ccl6 (pGL3-CCL6-luc)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Retinoid X receptor ? controls innate inflammatory responses through the up-regulation of chemokine expression

    doi: 10.1073/pnas.0913545107

    Figure Lengend Snippet: Transcription of Ccl6 and Ccl9 is regulated by RXRα via DR-1 rexinoid response elements. ( A and B ) RAW 264.7 macrophages were cotransfected with a luciferase reporter plasmid under the transcriptional control of either the Ccl6 (pGL3-CCL6-luc)

    Article Snippet: Anti-RXRα (D-20, sc-553) and anti-RXRDN-197 (sc-774) (Santa Cruz Biotechnology) antibodies were used in combination.

    Techniques: Luciferase, Plasmid Preparation

    Loss of RXRα in macrophages reduces Ccl6 and Ccl9 chemokine expression. ( A ) Q-PCR analysis of Ccl6 and Ccl9 mRNA expression in RXRα KO peritoneal macrophages. *, P ≤ 0.05 compared with WT. ( B ) Q-PCR analysis of Ccl6 and Ccl9 gene

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Retinoid X receptor ? controls innate inflammatory responses through the up-regulation of chemokine expression

    doi: 10.1073/pnas.0913545107

    Figure Lengend Snippet: Loss of RXRα in macrophages reduces Ccl6 and Ccl9 chemokine expression. ( A ) Q-PCR analysis of Ccl6 and Ccl9 mRNA expression in RXRα KO peritoneal macrophages. *, P ≤ 0.05 compared with WT. ( B ) Q-PCR analysis of Ccl6 and Ccl9 gene

    Article Snippet: Anti-RXRα (D-20, sc-553) and anti-RXRDN-197 (sc-774) (Santa Cruz Biotechnology) antibodies were used in combination.

    Techniques: Expressing, Polymerase Chain Reaction

    RXRα deficiency impairs leukocyte recruitment and decreases CCL6 and CCL9 expression during peritonitis induced by thioglycollate or zymosan. ( A ) ELISA analysis of CCL6 and CCL9 in peritoneal exudates collected from WT and RXRα KO mice

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Retinoid X receptor ? controls innate inflammatory responses through the up-regulation of chemokine expression

    doi: 10.1073/pnas.0913545107

    Figure Lengend Snippet: RXRα deficiency impairs leukocyte recruitment and decreases CCL6 and CCL9 expression during peritonitis induced by thioglycollate or zymosan. ( A ) ELISA analysis of CCL6 and CCL9 in peritoneal exudates collected from WT and RXRα KO mice

    Article Snippet: Anti-RXRα (D-20, sc-553) and anti-RXRDN-197 (sc-774) (Santa Cruz Biotechnology) antibodies were used in combination.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Mouse Assay

    Delayed CLP- and endotoxin-induced mortality in RXRα KO mice is associated with decreased levels of CCL6 and CCL9. ( A ) Decreased CCL6 and CCL9 in peritoneal exudates and plasma from WT and RXRα KO mice. Peritoneal exudates and plasma were

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Retinoid X receptor ? controls innate inflammatory responses through the up-regulation of chemokine expression

    doi: 10.1073/pnas.0913545107

    Figure Lengend Snippet: Delayed CLP- and endotoxin-induced mortality in RXRα KO mice is associated with decreased levels of CCL6 and CCL9. ( A ) Decreased CCL6 and CCL9 in peritoneal exudates and plasma from WT and RXRα KO mice. Peritoneal exudates and plasma were

    Article Snippet: Anti-RXRα (D-20, sc-553) and anti-RXRDN-197 (sc-774) (Santa Cruz Biotechnology) antibodies were used in combination.

    Techniques: Mouse Assay

    Induction of apoM gene transcription by retinoic acid receptors RXR and RAR. A , HepG2 cells were infected with a control adenovirus expressing GFP ( Ad-GFP ) or with a recombinant adenovirus expressing RXRα ( Ad-RXR ) at an m.o.i. of 10 for 24 h and

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of Human Apolipoprotein M Gene Expression by Orphan and Ligand-dependent Nuclear Receptors *

    doi: 10.1074/jbc.M110.131771

    Figure Lengend Snippet: Induction of apoM gene transcription by retinoic acid receptors RXR and RAR. A , HepG2 cells were infected with a control adenovirus expressing GFP ( Ad-GFP ) or with a recombinant adenovirus expressing RXRα ( Ad-RXR ) at an m.o.i. of 10 for 24 h and

    Article Snippet: Anti-HNF-4α (C-19), anti-RXRα (D-20), anti-RARα (C-20), anti-LXR (H-144), anti-TRβ1 (J51), and anti-PPARα (H-98) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Infection, Expressing, Recombinant

    RARγ, RXRα, H3K27ac, p300, and PolII-CTD association with specific Meis1 elements. ChIP assays of Meis1 P refseq (pp) and Meis1 downstream elements (DS1 and DS2) in WT and RARγ −/− ES cells to evaluate the association

    Journal: Journal of Cell Science

    Article Title: RAR? is essential for retinoic acid induced chromatin remodeling and transcriptional activation in embryonic stem cells

    doi: 10.1242/jcs.119701

    Figure Lengend Snippet: RARγ, RXRα, H3K27ac, p300, and PolII-CTD association with specific Meis1 elements. ChIP assays of Meis1 P refseq (pp) and Meis1 downstream elements (DS1 and DS2) in WT and RARγ −/− ES cells to evaluate the association

    Article Snippet: Anti-RXRα (D-20, sc-553), anti-p300 (N-15, sc-584), and anti-IgG (sc-2030) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Chromatin Immunoprecipitation

    Correlation of gender differential RXRα, Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Correlation of gender differential RXRα, Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: RNA Expression, Binding Assay, Expressing

    Gender differential responsiveness to RXRα activation in vivo. Male and female mice were gavaged once daily for 5 days with the RXRα ligand LG268. A) Relative RNA levels in male and female mouse liver as determined by QPCR in response to RXRα activation by LG268 for selected male and female enriched genes. B) RXRα occupancy in response to RXRα activation on selected male and female enriched genes (see also Fig. S5 and S6 ). *p

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Gender differential responsiveness to RXRα activation in vivo. Male and female mice were gavaged once daily for 5 days with the RXRα ligand LG268. A) Relative RNA levels in male and female mouse liver as determined by QPCR in response to RXRα activation by LG268 for selected male and female enriched genes. B) RXRα occupancy in response to RXRα activation on selected male and female enriched genes (see also Fig. S5 and S6 ). *p

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Activation Assay, In Vivo, Mouse Assay, Real-time Polymerase Chain Reaction

    Genome wide mapping of RXRα and Pol2 binding sites in male and female mouse liver. A) Venn diagrams show number of RXRα binding sites in male and female livers with 37602 binding sites shared between genders, and 10243 unique RXRα male and 9275 unique RXRα female binding sites (see also Table S1A and S1B in File S1 ). B) Venn diagrams show number of Pol2 binding sites in male and female livers, with 19307 binding sites shared between genders, and 6037 unique male and 1332 unique female Pol2 binding sites. C) Venn diagrams representing number of genes associated with RXRα peaks shown in A), with 11660 genes shared between genders, and 1099 unique genes for male and 1021 genes for female. D) Western blot analysis shows increased nuclear RXRα protein levels in female mouse liver compared to male mouse liver. E) Number of RXRα peaks as stratified by peak height (see also Table S2A and S2B in File S1 ). F) Genomic positions of RXRα binding sites in male and female mouse liver. Data expressed as percentage of total peaks per gender. RXRα binding was significantly increased at TSS (+/−500 bp), TES, within exons and introns with a depletion in binding compared to the expected number of sites based on random distribution (see also Fig. S1A and S1B ).

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Genome wide mapping of RXRα and Pol2 binding sites in male and female mouse liver. A) Venn diagrams show number of RXRα binding sites in male and female livers with 37602 binding sites shared between genders, and 10243 unique RXRα male and 9275 unique RXRα female binding sites (see also Table S1A and S1B in File S1 ). B) Venn diagrams show number of Pol2 binding sites in male and female livers, with 19307 binding sites shared between genders, and 6037 unique male and 1332 unique female Pol2 binding sites. C) Venn diagrams representing number of genes associated with RXRα peaks shown in A), with 11660 genes shared between genders, and 1099 unique genes for male and 1021 genes for female. D) Western blot analysis shows increased nuclear RXRα protein levels in female mouse liver compared to male mouse liver. E) Number of RXRα peaks as stratified by peak height (see also Table S2A and S2B in File S1 ). F) Genomic positions of RXRα binding sites in male and female mouse liver. Data expressed as percentage of total peaks per gender. RXRα binding was significantly increased at TSS (+/−500 bp), TES, within exons and introns with a depletion in binding compared to the expected number of sites based on random distribution (see also Fig. S1A and S1B ).

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Genome Wide, Binding Assay, Western Blot

    RXRα and Pol2 binding sites in mouse liver 10 kB surrounding the Transcriptional Start Sites. A) Venn diagram showing number of genes with shared RXRα regulation between male and female within 10 kB up and downstream of TSS, with 1285 genes found in male only and 1152 regulated by RXRα in females (see also Table S3A–D in File S1 ). B) Ontology analysis and pathway analysis of RXRα peaks with score > 6 or

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: RXRα and Pol2 binding sites in mouse liver 10 kB surrounding the Transcriptional Start Sites. A) Venn diagram showing number of genes with shared RXRα regulation between male and female within 10 kB up and downstream of TSS, with 1285 genes found in male only and 1152 regulated by RXRα in females (see also Table S3A–D in File S1 ). B) Ontology analysis and pathway analysis of RXRα peaks with score > 6 or

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Binding Assay

    Sexual dimorphic RXRα-dependent gene regulation in mouse liver. A) Scatter plot of RXRα scores correlating with Pol2 scores. Genes identified having a RXRα and Pol2 score > 6 for male and -

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Sexual dimorphic RXRα-dependent gene regulation in mouse liver. A) Scatter plot of RXRα scores correlating with Pol2 scores. Genes identified having a RXRα and Pol2 score > 6 for male and -

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques:

    Representatives of gender differential RXRαregulated genes. A) Predominant RXRα and Pol2 binding for Cyp7b1 in male mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Cyp7b1 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Cyp7b1 TSS. Male enriched RXRα peaks are indicated by arrows P1, P2 and P3. Right-hand panel: Sexual dimorphic RNA levels of Cyp7b1 were confirmed by qPCR (n = 5–6) (see also Fig. S2 and S3A–G ). B) Predominant RXRα and Pol2 binding for Pnpla3 in female mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Pnpla3 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Pnpla3 TSS. Female enriched RXRα peaks are indicated by arrows P1, P2, P3 and P4. Right-hand panel: Sexual dimorphic RNA levels of Pnpla3 were confirmed by qPCR (n = 5–6) (see also Fig. S2 , S3A–G , S7 and S8). *p

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Representatives of gender differential RXRαregulated genes. A) Predominant RXRα and Pol2 binding for Cyp7b1 in male mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Cyp7b1 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Cyp7b1 TSS. Male enriched RXRα peaks are indicated by arrows P1, P2 and P3. Right-hand panel: Sexual dimorphic RNA levels of Cyp7b1 were confirmed by qPCR (n = 5–6) (see also Fig. S2 and S3A–G ). B) Predominant RXRα and Pol2 binding for Pnpla3 in female mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Pnpla3 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Pnpla3 TSS. Female enriched RXRα peaks are indicated by arrows P1, P2, P3 and P4. Right-hand panel: Sexual dimorphic RNA levels of Pnpla3 were confirmed by qPCR (n = 5–6) (see also Fig. S2 , S3A–G , S7 and S8). *p

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Binding Assay, Real-time Polymerase Chain Reaction