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Santa Cruz Biotechnology anti rxrα d 20
Anti Rxrα D 20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti rxrα d 20/product/Santa Cruz Biotechnology
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti rxrα d 20 - by Bioz Stars, 2019-10
85/100 stars

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Immunoprecipitation:

Article Title: SP1 and RARα regulate AGAP2 expression in cancer
Article Snippet: The chromatin was sheared to 100–600 bp by sonication using an AFA Focused-Ultrasonicator (S220- Series from CovarisTM ) at 6 × 60 second on-off pulses at 4 °C. .. Afterwards, crosslinked proteins of interest were immunoprecipitated with 2 μg of either anti-RARα (C-20), anti-RXRα (D-20) (both from Santa Cruz Biotechnology), anti-PCAF (C14G9, Cell Signaling), or 1 μg of anti-SP1 (D4C3, Cell Signalling). .. Anti-Pol II (N-20, Santa Cruz Biotechnology) was used as positive control and rabbit IgG (Invitrogen) as negative control.

Purification:

Article Title: SP1 and RARα regulate AGAP2 expression in cancer
Article Snippet: Afterwards, crosslinked proteins of interest were immunoprecipitated with 2 μg of either anti-RARα (C-20), anti-RXRα (D-20) (both from Santa Cruz Biotechnology), anti-PCAF (C14G9, Cell Signaling), or 1 μg of anti-SP1 (D4C3, Cell Signalling). .. Anti-Pol II (N-20, Santa Cruz Biotechnology) was used as positive control and rabbit IgG (Invitrogen) as negative control.

Article Title: Differential Role of Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein in Toll-Like Receptor 2-Mediated Regulation of Gene Expression of Hepatic Cytokines and Drug-Metabolizing Enzymes
Article Snippet: Highly purified LTA ( Staphylococcus aureus ) was purchased from InvivoGen (San Diego, CA) and freshly diluted to the desired concentration in pyrogen-free 0.9% saline before injection. .. Anti-JNK, phospho-JNK, anti-IκB, β-actin (Cell Signaling Technology, Danvers, MA), and anti-RXRα (D-20) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) were used according to the manufacturer's instructions.

Real-time Polymerase Chain Reaction:

Article Title: SP1 and RARα regulate AGAP2 expression in cancer
Article Snippet: Afterwards, crosslinked proteins of interest were immunoprecipitated with 2 μg of either anti-RARα (C-20), anti-RXRα (D-20) (both from Santa Cruz Biotechnology), anti-PCAF (C14G9, Cell Signaling), or 1 μg of anti-SP1 (D4C3, Cell Signalling). .. Anti-Pol II (N-20, Santa Cruz Biotechnology) was used as positive control and rabbit IgG (Invitrogen) as negative control.

Article Title: Differential Role of Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein in Toll-Like Receptor 2-Mediated Regulation of Gene Expression of Hepatic Cytokines and Drug-Metabolizing Enzymes
Article Snippet: Anti-JNK, phospho-JNK, anti-IκB, β-actin (Cell Signaling Technology, Danvers, MA), and anti-RXRα (D-20) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) were used according to the manufacturer's instructions. .. Anti-JNK, phospho-JNK, anti-IκB, β-actin (Cell Signaling Technology, Danvers, MA), and anti-RXRα (D-20) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) were used according to the manufacturer's instructions.

Concentration Assay:

Article Title: Differential Role of Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein in Toll-Like Receptor 2-Mediated Regulation of Gene Expression of Hepatic Cytokines and Drug-Metabolizing Enzymes
Article Snippet: Highly purified LTA ( Staphylococcus aureus ) was purchased from InvivoGen (San Diego, CA) and freshly diluted to the desired concentration in pyrogen-free 0.9% saline before injection. .. Anti-JNK, phospho-JNK, anti-IκB, β-actin (Cell Signaling Technology, Danvers, MA), and anti-RXRα (D-20) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) were used according to the manufacturer's instructions.

Cell Fractionation:

Article Title: Rosiglitazone attenuates suppression of RXR?-dependent gene expression in inflamed liver
Article Snippet: Paragraph title: 2.4. Cell fractionation and immunoblotting ... The following antibodies were used in immunoblot analysis: JNK, phospho-JNK and phospho-c-Jun antibodies (Ser 63) (Cell Signaling, Beverly, MA), IκBα and anti-RXRα (D-20) (Santa Cruz Biotechnology, Santa Cruz, CA).

Sonication:

Article Title: SP1 and RARα regulate AGAP2 expression in cancer
Article Snippet: The chromatin was sheared to 100–600 bp by sonication using an AFA Focused-Ultrasonicator (S220- Series from CovarisTM ) at 6 × 60 second on-off pulses at 4 °C. .. Afterwards, crosslinked proteins of interest were immunoprecipitated with 2 μg of either anti-RARα (C-20), anti-RXRα (D-20) (both from Santa Cruz Biotechnology), anti-PCAF (C14G9, Cell Signaling), or 1 μg of anti-SP1 (D4C3, Cell Signalling).

Injection:

Article Title: Differential Role of Toll-Interleukin 1 Receptor Domain-Containing Adaptor Protein in Toll-Like Receptor 2-Mediated Regulation of Gene Expression of Hepatic Cytokines and Drug-Metabolizing Enzymes
Article Snippet: Highly purified LTA ( Staphylococcus aureus ) was purchased from InvivoGen (San Diego, CA) and freshly diluted to the desired concentration in pyrogen-free 0.9% saline before injection. .. Anti-JNK, phospho-JNK, anti-IκB, β-actin (Cell Signaling Technology, Danvers, MA), and anti-RXRα (D-20) (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) were used according to the manufacturer's instructions.

Chromatin Immunoprecipitation:

Article Title: SP1 and RARα regulate AGAP2 expression in cancer
Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Afterwards, crosslinked proteins of interest were immunoprecipitated with 2 μg of either anti-RARα (C-20), anti-RXRα (D-20) (both from Santa Cruz Biotechnology), anti-PCAF (C14G9, Cell Signaling), or 1 μg of anti-SP1 (D4C3, Cell Signalling).

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    Santa Cruz Biotechnology chip quality anti rxrα antibody
    Correlation of gender differential <t>RXRα,</t> Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).
    Chip Quality Anti Rxrα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 81/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chip quality anti rxrα antibody/product/Santa Cruz Biotechnology
    Average 81 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    chip quality anti rxrα antibody - by Bioz Stars, 2019-10
    81/100 stars
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    Correlation of gender differential RXRα, Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Correlation of gender differential RXRα, Pol2 and RNA expression levels. A) Correlation of RXRα and Pol2 binding with gene expression levels. B) Scatterplot representation of gender differential RXRα binding with gender differential gene expression on the left-hand side, and scatterplot representation of gender differential Pol2 binding with gender differential gene expression on the right-hand side. C) Genes with a gender specific positive correlation between RXRα binding Pol2 binding and changes in RNA levels (see also Fig. S4A–B and Table S6A–H in File S1 ).

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: RNA Expression, Binding Assay, Expressing

    Gender differential responsiveness to RXRα activation in vivo. Male and female mice were gavaged once daily for 5 days with the RXRα ligand LG268. A) Relative RNA levels in male and female mouse liver as determined by QPCR in response to RXRα activation by LG268 for selected male and female enriched genes. B) RXRα occupancy in response to RXRα activation on selected male and female enriched genes (see also Fig. S5 and S6 ). *p

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Gender differential responsiveness to RXRα activation in vivo. Male and female mice were gavaged once daily for 5 days with the RXRα ligand LG268. A) Relative RNA levels in male and female mouse liver as determined by QPCR in response to RXRα activation by LG268 for selected male and female enriched genes. B) RXRα occupancy in response to RXRα activation on selected male and female enriched genes (see also Fig. S5 and S6 ). *p

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Activation Assay, In Vivo, Mouse Assay, Real-time Polymerase Chain Reaction

    Genome wide mapping of RXRα and Pol2 binding sites in male and female mouse liver. A) Venn diagrams show number of RXRα binding sites in male and female livers with 37602 binding sites shared between genders, and 10243 unique RXRα male and 9275 unique RXRα female binding sites (see also Table S1A and S1B in File S1 ). B) Venn diagrams show number of Pol2 binding sites in male and female livers, with 19307 binding sites shared between genders, and 6037 unique male and 1332 unique female Pol2 binding sites. C) Venn diagrams representing number of genes associated with RXRα peaks shown in A), with 11660 genes shared between genders, and 1099 unique genes for male and 1021 genes for female. D) Western blot analysis shows increased nuclear RXRα protein levels in female mouse liver compared to male mouse liver. E) Number of RXRα peaks as stratified by peak height (see also Table S2A and S2B in File S1 ). F) Genomic positions of RXRα binding sites in male and female mouse liver. Data expressed as percentage of total peaks per gender. RXRα binding was significantly increased at TSS (+/−500 bp), TES, within exons and introns with a depletion in binding compared to the expected number of sites based on random distribution (see also Fig. S1A and S1B ).

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Genome wide mapping of RXRα and Pol2 binding sites in male and female mouse liver. A) Venn diagrams show number of RXRα binding sites in male and female livers with 37602 binding sites shared between genders, and 10243 unique RXRα male and 9275 unique RXRα female binding sites (see also Table S1A and S1B in File S1 ). B) Venn diagrams show number of Pol2 binding sites in male and female livers, with 19307 binding sites shared between genders, and 6037 unique male and 1332 unique female Pol2 binding sites. C) Venn diagrams representing number of genes associated with RXRα peaks shown in A), with 11660 genes shared between genders, and 1099 unique genes for male and 1021 genes for female. D) Western blot analysis shows increased nuclear RXRα protein levels in female mouse liver compared to male mouse liver. E) Number of RXRα peaks as stratified by peak height (see also Table S2A and S2B in File S1 ). F) Genomic positions of RXRα binding sites in male and female mouse liver. Data expressed as percentage of total peaks per gender. RXRα binding was significantly increased at TSS (+/−500 bp), TES, within exons and introns with a depletion in binding compared to the expected number of sites based on random distribution (see also Fig. S1A and S1B ).

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Genome Wide, Binding Assay, Western Blot

    RXRα and Pol2 binding sites in mouse liver 10 kB surrounding the Transcriptional Start Sites. A) Venn diagram showing number of genes with shared RXRα regulation between male and female within 10 kB up and downstream of TSS, with 1285 genes found in male only and 1152 regulated by RXRα in females (see also Table S3A–D in File S1 ). B) Ontology analysis and pathway analysis of RXRα peaks with score > 6 or

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: RXRα and Pol2 binding sites in mouse liver 10 kB surrounding the Transcriptional Start Sites. A) Venn diagram showing number of genes with shared RXRα regulation between male and female within 10 kB up and downstream of TSS, with 1285 genes found in male only and 1152 regulated by RXRα in females (see also Table S3A–D in File S1 ). B) Ontology analysis and pathway analysis of RXRα peaks with score > 6 or

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Binding Assay

    Sexual dimorphic RXRα-dependent gene regulation in mouse liver. A) Scatter plot of RXRα scores correlating with Pol2 scores. Genes identified having a RXRα and Pol2 score > 6 for male and -

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Sexual dimorphic RXRα-dependent gene regulation in mouse liver. A) Scatter plot of RXRα scores correlating with Pol2 scores. Genes identified having a RXRα and Pol2 score > 6 for male and -

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques:

    Representatives of gender differential RXRαregulated genes. A) Predominant RXRα and Pol2 binding for Cyp7b1 in male mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Cyp7b1 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Cyp7b1 TSS. Male enriched RXRα peaks are indicated by arrows P1, P2 and P3. Right-hand panel: Sexual dimorphic RNA levels of Cyp7b1 were confirmed by qPCR (n = 5–6) (see also Fig. S2 and S3A–G ). B) Predominant RXRα and Pol2 binding for Pnpla3 in female mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Pnpla3 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Pnpla3 TSS. Female enriched RXRα peaks are indicated by arrows P1, P2, P3 and P4. Right-hand panel: Sexual dimorphic RNA levels of Pnpla3 were confirmed by qPCR (n = 5–6) (see also Fig. S2 , S3A–G , S7 and S8). *p

    Journal: PLoS ONE

    Article Title: Sexually Dimorphic Genome-Wide Binding of Retinoid X Receptor alpha (RXR?) Determines Male-Female Differences in the Expression of Hepatic Lipid Processing Genes in Mice

    doi: 10.1371/journal.pone.0071538

    Figure Lengend Snippet: Representatives of gender differential RXRαregulated genes. A) Predominant RXRα and Pol2 binding for Cyp7b1 in male mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Cyp7b1 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Cyp7b1 TSS. Male enriched RXRα peaks are indicated by arrows P1, P2 and P3. Right-hand panel: Sexual dimorphic RNA levels of Cyp7b1 were confirmed by qPCR (n = 5–6) (see also Fig. S2 and S3A–G ). B) Predominant RXRα and Pol2 binding for Pnpla3 in female mouse liver. Left-hand panel: Top 2 tracks show Pol2 binding along the Pnpla3 gene-span in male and female mouse liver. Bottom 2 tracks show RXRα peaks binding upstream of the TSS of Pnpla3 TSS. Female enriched RXRα peaks are indicated by arrows P1, P2, P3 and P4. Right-hand panel: Sexual dimorphic RNA levels of Pnpla3 were confirmed by qPCR (n = 5–6) (see also Fig. S2 , S3A–G , S7 and S8). *p

    Article Snippet: ChIP-quality anti-RXRα antibody (D-20,sc-774X, Santa Cruz Biotechnology, Santa Cruz, CA) and RNA Pol2 (ab24758, Abcam) were used.

    Techniques: Binding Assay, Real-time Polymerase Chain Reaction

    Direct protein-protein interactions between NPDC-1 and RXR. Bacterially expressed flag epitope tagged NPDC-1 [NPDC(F)] (40 μg) was incubated with glutathione-agarose bound GST/human RXRα (GST-RXR) fusion protein in the absence or presence of bacterially expressed human RARγ (RAR) (60 μg) or in the presence of 9-cis retinoic acid (9cRA) (10 -6 M) as indicated. NPDC(F) was also incubated with glutathione-agarose bound GST. After extensive washing, the retained proteins were resolved by gel electrophoresis and analyzed by immunoblot with anti-flag M2 antibodies. One percent (0.4 μg) of the available NPDC(F) input was also electrophoresed and a unique immunoreactive band of approximately 45 kDa representing NPDC(F) is indicated by an arrow. The position of prestained molecular weight standards are shown.

    Journal: Nuclear Receptor

    Article Title: A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

    doi: 10.1186/1478-1336-1-7

    Figure Lengend Snippet: Direct protein-protein interactions between NPDC-1 and RXR. Bacterially expressed flag epitope tagged NPDC-1 [NPDC(F)] (40 μg) was incubated with glutathione-agarose bound GST/human RXRα (GST-RXR) fusion protein in the absence or presence of bacterially expressed human RARγ (RAR) (60 μg) or in the presence of 9-cis retinoic acid (9cRA) (10 -6 M) as indicated. NPDC(F) was also incubated with glutathione-agarose bound GST. After extensive washing, the retained proteins were resolved by gel electrophoresis and analyzed by immunoblot with anti-flag M2 antibodies. One percent (0.4 μg) of the available NPDC(F) input was also electrophoresed and a unique immunoreactive band of approximately 45 kDa representing NPDC(F) is indicated by an arrow. The position of prestained molecular weight standards are shown.

    Article Snippet: Filters were probed with anti-HA (Cell Signaling), anti-RARβ (C-19, Santa Cruz), or anti-RXRα (D-20, Santa Cruz).

    Techniques: FLAG-tag, Incubation, Nucleic Acid Electrophoresis, Molecular Weight

    RXR binds the amino terminus of hNPDC-1 . Approximately 10 μg of recombinant S-tagged hNPDC-1 or NPDC-1 deletion mutant (A) was pre-coupled to S-protein to generate a 50% slurry of NPDC-1-beads. Reactions containing 30 μl NPDC-beads, 5 μg of recombinant GST-RXRα and 300 μl of 1X gel-shift buffer were incubated at 4°C for 1 hour with rotation. S-protein pellets were collected by centrifugation and washed 3 times in gel-shift buffer. Proteins were released from the pellet by resuspending S-protein agarose in 2X SDS-PAGE sample buffer. The amount of GST-RXRα bound to beads was analyzed by Western blotting onto nitrocellulose filters and probing blots with anti-RXRα (B, top panel). To normalize for unequal expression of the various deletion mutants, duplicate reactions without GST-RXRα were performed and subjected to coomassie staining instead of immunoblotting, and NIH Image Software was used to normalize the bands in the immunoblot (B, top panel) to the amount of S-tagged NPDC-1 precipitated in the coomassie stained gel. Normalized binding is depicted in B, bottom panel.

    Journal: Nuclear Receptor

    Article Title: A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

    doi: 10.1186/1478-1336-1-7

    Figure Lengend Snippet: RXR binds the amino terminus of hNPDC-1 . Approximately 10 μg of recombinant S-tagged hNPDC-1 or NPDC-1 deletion mutant (A) was pre-coupled to S-protein to generate a 50% slurry of NPDC-1-beads. Reactions containing 30 μl NPDC-beads, 5 μg of recombinant GST-RXRα and 300 μl of 1X gel-shift buffer were incubated at 4°C for 1 hour with rotation. S-protein pellets were collected by centrifugation and washed 3 times in gel-shift buffer. Proteins were released from the pellet by resuspending S-protein agarose in 2X SDS-PAGE sample buffer. The amount of GST-RXRα bound to beads was analyzed by Western blotting onto nitrocellulose filters and probing blots with anti-RXRα (B, top panel). To normalize for unequal expression of the various deletion mutants, duplicate reactions without GST-RXRα were performed and subjected to coomassie staining instead of immunoblotting, and NIH Image Software was used to normalize the bands in the immunoblot (B, top panel) to the amount of S-tagged NPDC-1 precipitated in the coomassie stained gel. Normalized binding is depicted in B, bottom panel.

    Article Snippet: Filters were probed with anti-HA (Cell Signaling), anti-RARβ (C-19, Santa Cruz), or anti-RXRα (D-20, Santa Cruz).

    Techniques: Recombinant, Mutagenesis, Electrophoretic Mobility Shift Assay, Incubation, Centrifugation, SDS Page, Western Blot, Expressing, Staining, Software, Binding Assay

    NPDC-1 Alters RARβ••••α and RXRα DNA binding properties. Oligonucleotides corresponding to the consensus RARβ•RARE (DR-5: A) or the consensus RXRα RARE (DR-1: B C) were end-labeled with 32- P by T4 polynucleotide kinase according to the manufacture's instructions. The resultant DNA binding probes were incubated with PC12 lysate (A), recombinant NPDC-1 and recombinant RXRα (B C) as indicated. DNA:protein complexes were resolved on non-denaturing PAGE gels which were subsequently exposed to Kodak Xar-5 film. In C, the presence of NPDC-1 within the RXR gel-shift complex was assayed by supershift. Antibodies specific for either NPDC-1 or RXR were used to identify proteins present in the complex.

    Journal: Nuclear Receptor

    Article Title: A neuronal-specific differentiation protein that directly modulates retinoid receptor transcriptional activation

    doi: 10.1186/1478-1336-1-7

    Figure Lengend Snippet: NPDC-1 Alters RARβ••••α and RXRα DNA binding properties. Oligonucleotides corresponding to the consensus RARβ•RARE (DR-5: A) or the consensus RXRα RARE (DR-1: B C) were end-labeled with 32- P by T4 polynucleotide kinase according to the manufacture's instructions. The resultant DNA binding probes were incubated with PC12 lysate (A), recombinant NPDC-1 and recombinant RXRα (B C) as indicated. DNA:protein complexes were resolved on non-denaturing PAGE gels which were subsequently exposed to Kodak Xar-5 film. In C, the presence of NPDC-1 within the RXR gel-shift complex was assayed by supershift. Antibodies specific for either NPDC-1 or RXR were used to identify proteins present in the complex.

    Article Snippet: Filters were probed with anti-HA (Cell Signaling), anti-RARβ (C-19, Santa Cruz), or anti-RXRα (D-20, Santa Cruz).

    Techniques: Binding Assay, Labeling, Incubation, Recombinant, Polyacrylamide Gel Electrophoresis, Electrophoretic Mobility Shift Assay

    Real-time RT-PCR analysis of CYP1A1 mRNA expression in Caco-2 cells following stimulation with different nuclear receptor agonists. Cells were treated with agonists of the AHR, the different retinoid receptors, or combinations of both. Combination of RXRα and AHR activation yields over-additive effects. Mean + SD (n ≥ 3 independent experiments) are shown. Statistical significance is indicated as follows: *, p

    Journal: EXCLI Journal

    Article Title: The aryl hydrocarbon receptor and retinoid receptors cross-talk at the CYP1A1 promoter in vitro

    doi: 10.17179/excli2018-1147

    Figure Lengend Snippet: Real-time RT-PCR analysis of CYP1A1 mRNA expression in Caco-2 cells following stimulation with different nuclear receptor agonists. Cells were treated with agonists of the AHR, the different retinoid receptors, or combinations of both. Combination of RXRα and AHR activation yields over-additive effects. Mean + SD (n ≥ 3 independent experiments) are shown. Statistical significance is indicated as follows: *, p

    Article Snippet: Immunoprecipitation was carried out with antibodies directed against acetyl-histone H3 (H3-K9/K14, 06-599; Upstate, Temecula, CA, USA), RXRα (D 20), RXRβ (C 20), and AHR (H 211) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Quantitative RT-PCR, Expressing, Activation Assay

    ChIP analysis of CYP1A1 promoter binding of H3Ac, AHR, RXRα and RXRβ

    Journal: EXCLI Journal

    Article Title: The aryl hydrocarbon receptor and retinoid receptors cross-talk at the CYP1A1 promoter in vitro

    doi: 10.17179/excli2018-1147

    Figure Lengend Snippet: ChIP analysis of CYP1A1 promoter binding of H3Ac, AHR, RXRα and RXRβ

    Article Snippet: Immunoprecipitation was carried out with antibodies directed against acetyl-histone H3 (H3-K9/K14, 06-599; Upstate, Temecula, CA, USA), RXRα (D 20), RXRβ (C 20), and AHR (H 211) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Chromatin Immunoprecipitation, Binding Assay

    Chromatin immunoprecipitation at the CYP1A1 promoter. Immunoprecipitation results with antibodies directed against acetylated histone H3 (H3Ac) (A), the AHR (B), RXRα (C), and RXRβ (D) are presented. The dashed line indicates the level at which no enrichment of the respective promoter region is present. Negative controls (amplification of the PAX5 coding region not responsive to the AHR or retinoid receptors) are shown as white bars for comparison. Mean + SD (n ≥ 4 independent experiments) are shown. Statistical significance is indicated as follows: a , p

    Journal: EXCLI Journal

    Article Title: The aryl hydrocarbon receptor and retinoid receptors cross-talk at the CYP1A1 promoter in vitro

    doi: 10.17179/excli2018-1147

    Figure Lengend Snippet: Chromatin immunoprecipitation at the CYP1A1 promoter. Immunoprecipitation results with antibodies directed against acetylated histone H3 (H3Ac) (A), the AHR (B), RXRα (C), and RXRβ (D) are presented. The dashed line indicates the level at which no enrichment of the respective promoter region is present. Negative controls (amplification of the PAX5 coding region not responsive to the AHR or retinoid receptors) are shown as white bars for comparison. Mean + SD (n ≥ 4 independent experiments) are shown. Statistical significance is indicated as follows: a , p

    Article Snippet: Immunoprecipitation was carried out with antibodies directed against acetyl-histone H3 (H3-K9/K14, 06-599; Upstate, Temecula, CA, USA), RXRα (D 20), RXRβ (C 20), and AHR (H 211) (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Chromatin Immunoprecipitation, Immunoprecipitation, Amplification

    CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by RXRα/RARα requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase

    Journal:

    Article Title: The Corepressor CTBP2 Is a Coactivator of Retinoic Acid Receptor/Retinoid X Receptor in Retinoic Acid Signaling

    doi: 10.1128/MCB.01213-12

    Figure Lengend Snippet: CTBP2 is a transcriptional cofactor for RXR α/ RAR α. (A to C) Activation of a RARE-luciferase (RARE-Luc) reporter gene by RXRα/RARα requires CTBP2. The normalized luciferase activities shown represent ratios between luciferase

    Article Snippet: The following antibodies were used: anti-p300 (C-20, Sc585; Santa Cruz), histone H3 (ab1791; Abcam) and H3 acetyl antibodies (06-599; Upstate Millipore), normal rabbit IgG (Santa Cruz), anti-RXRα (D-20; Santa Cruz), anti-CTBP2 (612044; BD Transduction Laboratories), and anti-CD11b-fluorescein isothiocyanate (FITC) antibody (F2648; Sigma). siRNA for mouse Ctbp2 was purchased from Thermo Scientific (#MU-059787-01-0002; SiGenome mouse Ctbp2 ).

    Techniques: Activation Assay, Luciferase

    CTBP2 physically associates with RARα/RXRα and the RARE region of RA target gene promoters. (A) In vitro interaction of CTBP2 and RXRα. A GST pulldown assay was used to determine the binding between GST fusions of RARα

    Journal:

    Article Title: The Corepressor CTBP2 Is a Coactivator of Retinoic Acid Receptor/Retinoid X Receptor in Retinoic Acid Signaling

    doi: 10.1128/MCB.01213-12

    Figure Lengend Snippet: CTBP2 physically associates with RARα/RXRα and the RARE region of RA target gene promoters. (A) In vitro interaction of CTBP2 and RXRα. A GST pulldown assay was used to determine the binding between GST fusions of RARα

    Article Snippet: The following antibodies were used: anti-p300 (C-20, Sc585; Santa Cruz), histone H3 (ab1791; Abcam) and H3 acetyl antibodies (06-599; Upstate Millipore), normal rabbit IgG (Santa Cruz), anti-RXRα (D-20; Santa Cruz), anti-CTBP2 (612044; BD Transduction Laboratories), and anti-CD11b-fluorescein isothiocyanate (FITC) antibody (F2648; Sigma). siRNA for mouse Ctbp2 was purchased from Thermo Scientific (#MU-059787-01-0002; SiGenome mouse Ctbp2 ).

    Techniques: In Vitro, GST Pulldown Assay, Binding Assay