anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ripk3  (ProSci Incorporated)


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    ProSci Incorporated ripk3
    Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    A LLC-OVA cells were treated with DMSO or 5-AZA-dC (30 μM) for 72 hours prior to lysing cells for western blot. Data is representative of two independent experiments. B , C Transduced LLC-OVA cells (WT) were treated with DOX (1 μg/mL) for 8 h prior to treatment with either DMSO or MG132 (4 μM). B Cell death was measured by tracking YoYo1 (50 nM) uptake in cells via Incucyte. C Cell lysates were collected 4 h after treatment with MG132 for western blot. For B , C , data is representative of greater than three independent experiments. D , E WT cells were treated with DOX (1 μg/mL) for 7 hours, cells were then pre-treated with zVAD-fmk (20 μM) 30 mins prior to GSK’843 (20 μM) then MG132 (4 μM) after another 30 mins. D Cell death was monitored via Incucyte. E Cell lysates were collected 4 h after MG132 treatment for western blot. For D , E , data is representative of two independent experiments. F WT cells were treated with DMSO, MG132, DOX + DMSO, or DOX + MG132 and lysates were subjected to immunoprecipitation with anti-FLAG beads and subsequent western blot. IP indicates the immunoprecipitated fraction. WCL indicates the whole cell lysate prior to immunoprecipitation. Data is representative of two independent experiments. G , H WT, Casp8-KO, and MLKL-KO LLC-OVA cells were treated for 8 h with DOX (1 μg/mL) followed by treatment with MG132 (4 μM). G Cell death measured via Incucyte. H Cell lysates were collected at 4 hours following treatment with MG132 for western blot. For G , H , data is representative of two independent experiments. I , J WT or MLKL- and Casp8-KO (DKO) cells were treated for 8 hours with DOX (1 μg/mL) followed by treatment with MG132 (4 μM). I Cell death measured via Incucyte. J Cell lysates were collected at 4 hours following treatment with MG132 for western blot. For I , J , data is from a single experiment. K Graphical summary of <t>RIPK3-dependent</t> cell death in tumor cells following proteasome inhibition. Image was created with BioRender.com.
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ripk3/product/ProSci Incorporated
    Average 86 stars, based on 1 article reviews
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    anti ripk3 - by Bioz Stars, 2024-07
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    1) Product Images from "Necroptosis stimulates interferon-mediated protective anti-tumor immunity"

    Article Title: Necroptosis stimulates interferon-mediated protective anti-tumor immunity

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06801-8

    A LLC-OVA cells were treated with DMSO or 5-AZA-dC (30 μM) for 72 hours prior to lysing cells for western blot. Data is representative of two independent experiments. B , C Transduced LLC-OVA cells (WT) were treated with DOX (1 μg/mL) for 8 h prior to treatment with either DMSO or MG132 (4 μM). B Cell death was measured by tracking YoYo1 (50 nM) uptake in cells via Incucyte. C Cell lysates were collected 4 h after treatment with MG132 for western blot. For B , C , data is representative of greater than three independent experiments. D , E WT cells were treated with DOX (1 μg/mL) for 7 hours, cells were then pre-treated with zVAD-fmk (20 μM) 30 mins prior to GSK’843 (20 μM) then MG132 (4 μM) after another 30 mins. D Cell death was monitored via Incucyte. E Cell lysates were collected 4 h after MG132 treatment for western blot. For D , E , data is representative of two independent experiments. F WT cells were treated with DMSO, MG132, DOX + DMSO, or DOX + MG132 and lysates were subjected to immunoprecipitation with anti-FLAG beads and subsequent western blot. IP indicates the immunoprecipitated fraction. WCL indicates the whole cell lysate prior to immunoprecipitation. Data is representative of two independent experiments. G , H WT, Casp8-KO, and MLKL-KO LLC-OVA cells were treated for 8 h with DOX (1 μg/mL) followed by treatment with MG132 (4 μM). G Cell death measured via Incucyte. H Cell lysates were collected at 4 hours following treatment with MG132 for western blot. For G , H , data is representative of two independent experiments. I , J WT or MLKL- and Casp8-KO (DKO) cells were treated for 8 hours with DOX (1 μg/mL) followed by treatment with MG132 (4 μM). I Cell death measured via Incucyte. J Cell lysates were collected at 4 hours following treatment with MG132 for western blot. For I , J , data is from a single experiment. K Graphical summary of RIPK3-dependent cell death in tumor cells following proteasome inhibition. Image was created with BioRender.com.
    Figure Legend Snippet: A LLC-OVA cells were treated with DMSO or 5-AZA-dC (30 μM) for 72 hours prior to lysing cells for western blot. Data is representative of two independent experiments. B , C Transduced LLC-OVA cells (WT) were treated with DOX (1 μg/mL) for 8 h prior to treatment with either DMSO or MG132 (4 μM). B Cell death was measured by tracking YoYo1 (50 nM) uptake in cells via Incucyte. C Cell lysates were collected 4 h after treatment with MG132 for western blot. For B , C , data is representative of greater than three independent experiments. D , E WT cells were treated with DOX (1 μg/mL) for 7 hours, cells were then pre-treated with zVAD-fmk (20 μM) 30 mins prior to GSK’843 (20 μM) then MG132 (4 μM) after another 30 mins. D Cell death was monitored via Incucyte. E Cell lysates were collected 4 h after MG132 treatment for western blot. For D , E , data is representative of two independent experiments. F WT cells were treated with DMSO, MG132, DOX + DMSO, or DOX + MG132 and lysates were subjected to immunoprecipitation with anti-FLAG beads and subsequent western blot. IP indicates the immunoprecipitated fraction. WCL indicates the whole cell lysate prior to immunoprecipitation. Data is representative of two independent experiments. G , H WT, Casp8-KO, and MLKL-KO LLC-OVA cells were treated for 8 h with DOX (1 μg/mL) followed by treatment with MG132 (4 μM). G Cell death measured via Incucyte. H Cell lysates were collected at 4 hours following treatment with MG132 for western blot. For G , H , data is representative of two independent experiments. I , J WT or MLKL- and Casp8-KO (DKO) cells were treated for 8 hours with DOX (1 μg/mL) followed by treatment with MG132 (4 μM). I Cell death measured via Incucyte. J Cell lysates were collected at 4 hours following treatment with MG132 for western blot. For I , J , data is from a single experiment. K Graphical summary of RIPK3-dependent cell death in tumor cells following proteasome inhibition. Image was created with BioRender.com.

    Techniques Used: Western Blot, Immunoprecipitation, Inhibition

    anti ripk3 antibody  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3 antibody
    Anti Ripk3 Antibody, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ripk3/product/ProSci Incorporated
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    anti ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti ripk3
    Anti Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti mouse ripk3  (ProSci Incorporated)


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    ProSci Incorporated anti mouse ripk3
    Anti Mouse Ripk3, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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