anti rat trpv1  (Alomone Labs)


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    Structured Review

    Alomone Labs anti rat trpv1
    Immunofluorescence for CD88, interleukin-8 RA (IL-8RA) and transient receptor potential cation channel subfamily V member 1 <t>(TRPV1)</t> on 3rd day after administration of doxorubicin into mouse dorsal skin followed by 3-hrs compress. Box plot explanation: The median for each group is shown as a box plot with whiskers from minimum to maximum in (B, D and F). The interquartile range is shown as a box with the median marked as a horizontal line, minimum and maximum from lower and upper quartile represent error bar. (A B) Immunofluorescence for CD88 (A) and IL-8RA (C), and the number (per mm 2 ) of CD88 (B) and IL-8RA (D)-positive cells after 3-hrs cold or hot compresses. Immunofluorescence of TRPV1-positive cells (E) and the number (per mm 2 ) of TRPV1-positive nerve fascicles (F) after 3-hrs cold or hot compresses. [(a) TRPV1 (fluorescein isothiocyanate; FITC), (d) α-smooth muscle actin (α-SMA) (tetramethylrhodamine; TRITC), (g) merge TRPV1 (arrowheads); (b) TRPV1 (FITC), (e) α-SMA (TRITC), (h) merge TRPV1 (arrowheads); (c) TRPV1 (FITC), (f) α-SMA (TRITC), (i) merge TRPV1 (arrowheads)]. (a) control group. (b) cold compress group. (c) hot compress group in (A, C and E). Bar = 50 μm, *: P
    Anti Rat Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rat trpv1/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    anti rat trpv1 - by Bioz Stars, 2022-09
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    Images

    1) Product Images from "Effect of cold and hot compress on neutrophilic migration to the site of doxorubicin extravasation"

    Article Title: Effect of cold and hot compress on neutrophilic migration to the site of doxorubicin extravasation

    Journal: International Journal of Clinical and Experimental Pathology

    doi:

    Immunofluorescence for CD88, interleukin-8 RA (IL-8RA) and transient receptor potential cation channel subfamily V member 1 (TRPV1) on 3rd day after administration of doxorubicin into mouse dorsal skin followed by 3-hrs compress. Box plot explanation: The median for each group is shown as a box plot with whiskers from minimum to maximum in (B, D and F). The interquartile range is shown as a box with the median marked as a horizontal line, minimum and maximum from lower and upper quartile represent error bar. (A B) Immunofluorescence for CD88 (A) and IL-8RA (C), and the number (per mm 2 ) of CD88 (B) and IL-8RA (D)-positive cells after 3-hrs cold or hot compresses. Immunofluorescence of TRPV1-positive cells (E) and the number (per mm 2 ) of TRPV1-positive nerve fascicles (F) after 3-hrs cold or hot compresses. [(a) TRPV1 (fluorescein isothiocyanate; FITC), (d) α-smooth muscle actin (α-SMA) (tetramethylrhodamine; TRITC), (g) merge TRPV1 (arrowheads); (b) TRPV1 (FITC), (e) α-SMA (TRITC), (h) merge TRPV1 (arrowheads); (c) TRPV1 (FITC), (f) α-SMA (TRITC), (i) merge TRPV1 (arrowheads)]. (a) control group. (b) cold compress group. (c) hot compress group in (A, C and E). Bar = 50 μm, *: P
    Figure Legend Snippet: Immunofluorescence for CD88, interleukin-8 RA (IL-8RA) and transient receptor potential cation channel subfamily V member 1 (TRPV1) on 3rd day after administration of doxorubicin into mouse dorsal skin followed by 3-hrs compress. Box plot explanation: The median for each group is shown as a box plot with whiskers from minimum to maximum in (B, D and F). The interquartile range is shown as a box with the median marked as a horizontal line, minimum and maximum from lower and upper quartile represent error bar. (A B) Immunofluorescence for CD88 (A) and IL-8RA (C), and the number (per mm 2 ) of CD88 (B) and IL-8RA (D)-positive cells after 3-hrs cold or hot compresses. Immunofluorescence of TRPV1-positive cells (E) and the number (per mm 2 ) of TRPV1-positive nerve fascicles (F) after 3-hrs cold or hot compresses. [(a) TRPV1 (fluorescein isothiocyanate; FITC), (d) α-smooth muscle actin (α-SMA) (tetramethylrhodamine; TRITC), (g) merge TRPV1 (arrowheads); (b) TRPV1 (FITC), (e) α-SMA (TRITC), (h) merge TRPV1 (arrowheads); (c) TRPV1 (FITC), (f) α-SMA (TRITC), (i) merge TRPV1 (arrowheads)]. (a) control group. (b) cold compress group. (c) hot compress group in (A, C and E). Bar = 50 μm, *: P

    Techniques Used: Immunofluorescence

    Reverse transcription-polymerase chain reaction (RT-PCR) for CD88, interleukin-8 RA (IL-8RA), and transient receptor potential cation channel subfamily V member 1 (TRPV1). mRNAs of CD88, IL-8RA, and TRPV1 including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were expressed in skin tissue on the first day after doxorubicin administration. MM: molecular marker, Lane 1; positive control (brain), Lane 2; control (no compress), Lane 3; cold compress, Lane 4; hot compress, Lane 5; negative control.
    Figure Legend Snippet: Reverse transcription-polymerase chain reaction (RT-PCR) for CD88, interleukin-8 RA (IL-8RA), and transient receptor potential cation channel subfamily V member 1 (TRPV1). mRNAs of CD88, IL-8RA, and TRPV1 including glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were expressed in skin tissue on the first day after doxorubicin administration. MM: molecular marker, Lane 1; positive control (brain), Lane 2; control (no compress), Lane 3; cold compress, Lane 4; hot compress, Lane 5; negative control.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Marker, Positive Control, Negative Control

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    Alomone Labs rabbit anti trpv1
    Harpagophytum procumbens (HP) prevents upregulation of <t>TRPV1</t> in dorsal root ganglions (DRG) and attenuates chronic neuropathic pain. (a) Representative immunohistochemical images of TRPV1 (green) and NeuN (red) in DRGs at 3 weeks after LSS in each group. White scale bar = 200 μ m. (b) Percentage of TRPV1 + neurons that colabeled with NeuN + neurons in DRG. (c) Quantification of fluorescence intensity in TRPV1 + neuron within DRG. Data are expressed as the means ± SEM. Significant differences are indicated as #### p
    Rabbit Anti Trpv1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti trpv1/product/Alomone Labs
    Average 88 stars, based on 1 article reviews
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    rabbit anti trpv1 - by Bioz Stars, 2022-09
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    96
    Alomone Labs anti trpv1 vr1 antibody
    STZ injection upregulated the expression of <t>TRPV1</t> in DRG, whereas ALA downregulated TRPV1 expression. A, Western blots for TRPV1 of DRGs innervating the hindpaw from control and STZ injection rats. Bar graph indicated mean density relative of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and TRPV1 from CNT and STZ rats. STZ injection significantly enhanced expression of TRPV1 of DRGs innervating the hindpaw (N = 4 for each group, * P
    Anti Trpv1 Vr1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti trpv1 vr1 antibody/product/Alomone Labs
    Average 96 stars, based on 1 article reviews
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    93
    Alomone Labs rabbit anti trpv1 antibody
    IAN transection both in NP and non-NP groups changes the expression profile of <t>TRPV1</t> to myelinated neurons of a larger diameter. The total number of TG cells labeled for the fluoro-gold (FG + ) (A: NP group, B: Non-NP group); TG cells that labeled for TRPV1 and FG (TRPV1 + +FG + ) in 2-; 3-and 4-week NP groups and in sham-operated group (C: NP group, D: Non-NP group). The ratio of TRPV1 + +FG + to all FG+ positive cells (E: NP group, F: Non-NP group). n = 5 for each group, (ANOVA followed by the Student–Newman–Keuls test, *p
    Rabbit Anti Trpv1 Antibody, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti trpv1 antibody/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti trpv1 antibody - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Harpagophytum procumbens (HP) prevents upregulation of TRPV1 in dorsal root ganglions (DRG) and attenuates chronic neuropathic pain. (a) Representative immunohistochemical images of TRPV1 (green) and NeuN (red) in DRGs at 3 weeks after LSS in each group. White scale bar = 200 μ m. (b) Percentage of TRPV1 + neurons that colabeled with NeuN + neurons in DRG. (c) Quantification of fluorescence intensity in TRPV1 + neuron within DRG. Data are expressed as the means ± SEM. Significant differences are indicated as #### p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Harpagophytum procumbens Inhibits Iron Overload-Induced Oxidative Stress through Activation of Nrf2 Signaling in a Rat Model of Lumbar Spinal Stenosis

    doi: 10.1155/2022/3472443

    Figure Lengend Snippet: Harpagophytum procumbens (HP) prevents upregulation of TRPV1 in dorsal root ganglions (DRG) and attenuates chronic neuropathic pain. (a) Representative immunohistochemical images of TRPV1 (green) and NeuN (red) in DRGs at 3 weeks after LSS in each group. White scale bar = 200 μ m. (b) Percentage of TRPV1 + neurons that colabeled with NeuN + neurons in DRG. (c) Quantification of fluorescence intensity in TRPV1 + neuron within DRG. Data are expressed as the means ± SEM. Significant differences are indicated as #### p

    Article Snippet: Sections were incubated with primary antibodies against rabbit anti-CD68 (Abcam, 1 : 500), rabbit anti-TRPV1 (Alomone, Hadassah Ein Kerem, Israel, 1 : 100), guinea pig anti-NeuN (Synaptic Systems, Gottingen, Germany, 1 : 500), mouse antiferritin heavy chain (Santa Cruz Biotechnology, Santa Cruz, CA, USA, 1 : 400), mouse anti-iNOS (R & D Systems, Minneapolis, MN, USA, 1 : 100), rabbit anti-NRF2 (Abcam, 1 : 200), and mouse anti-NF200 (Millipore, Billerica, MA, USA, 1 : 200) overnight at 4°C.

    Techniques: Immunohistochemistry, Fluorescence

    STZ injection upregulated the expression of TRPV1 in DRG, whereas ALA downregulated TRPV1 expression. A, Western blots for TRPV1 of DRGs innervating the hindpaw from control and STZ injection rats. Bar graph indicated mean density relative of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and TRPV1 from CNT and STZ rats. STZ injection significantly enhanced expression of TRPV1 of DRGs innervating the hindpaw (N = 4 for each group, * P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes, et al. Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes

    doi: 10.1111/cns.13303

    Figure Lengend Snippet: STZ injection upregulated the expression of TRPV1 in DRG, whereas ALA downregulated TRPV1 expression. A, Western blots for TRPV1 of DRGs innervating the hindpaw from control and STZ injection rats. Bar graph indicated mean density relative of glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and TRPV1 from CNT and STZ rats. STZ injection significantly enhanced expression of TRPV1 of DRGs innervating the hindpaw (N = 4 for each group, * P

    Article Snippet: Mouse anti‐p65 (#6956, Cell Signaling Technology, 1:50) and rabbit anti‐TRPV1 (#ACC‐030, Alomone, 1:50) were used in this study.

    Techniques: Injection, Expressing, Western Blot

    TRPV1 and NF‐κB were co‐expressed in DRG neurons. A, NF‐κB‐positive cells were shown in red. B, TRPV1‐positive cells were shown in green. C, Merge of double labeling of TRPV1 and NF‐κB. Scale bar was 50 μm. D, Quantified analysis showed the majority of TRPV1 was co‐expressed with NF‐κB‐positive DRG neurons, and the majority of NF‐κB was also co‐expressed with TRPV1‐positive DRG neurons

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes, et al. Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes

    doi: 10.1111/cns.13303

    Figure Lengend Snippet: TRPV1 and NF‐κB were co‐expressed in DRG neurons. A, NF‐κB‐positive cells were shown in red. B, TRPV1‐positive cells were shown in green. C, Merge of double labeling of TRPV1 and NF‐κB. Scale bar was 50 μm. D, Quantified analysis showed the majority of TRPV1 was co‐expressed with NF‐κB‐positive DRG neurons, and the majority of NF‐κB was also co‐expressed with TRPV1‐positive DRG neurons

    Article Snippet: Mouse anti‐p65 (#6956, Cell Signaling Technology, 1:50) and rabbit anti‐TRPV1 (#ACC‐030, Alomone, 1:50) were used in this study.

    Techniques: Labeling

    ALA inhibited TRPV1 expression via suppressing NF‐κB. A, Western blots for NF‐κB in DRGs innervating the hindpaw from control and STZ‐induced diabetic rats. Bar graph involved mean density relative of β‐actin and NF‐κB from control and STZ‐induced diabetic rats. STZ injection significantly enhanced expression of NF‐κB (N = 4 for each group; * P

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes, et al. Alpha‐lipoic acid downregulates TRPV1 receptor via NF‐κB and attenuates neuropathic pain in rats with diabetes

    doi: 10.1111/cns.13303

    Figure Lengend Snippet: ALA inhibited TRPV1 expression via suppressing NF‐κB. A, Western blots for NF‐κB in DRGs innervating the hindpaw from control and STZ‐induced diabetic rats. Bar graph involved mean density relative of β‐actin and NF‐κB from control and STZ‐induced diabetic rats. STZ injection significantly enhanced expression of NF‐κB (N = 4 for each group; * P

    Article Snippet: Mouse anti‐p65 (#6956, Cell Signaling Technology, 1:50) and rabbit anti‐TRPV1 (#ACC‐030, Alomone, 1:50) were used in this study.

    Techniques: Expressing, Western Blot, Injection

    NGF induces a minor increase in Ca 2+ - and SNARE-dependent CGRP release, whereas it greatly enhances the CAP-evoked exocytosis which is blocked by BoNT/A and/EA at low [CAP] but only abolished by BoNT/EA at higher [CAP]. ( A ) Illustrates the effect of acute NGF on CGRP exocytosis from control neonatal rat TGNs starved of the neurotrophin for 2 days, and ( B ) in TGNs pre-treated with BoNT/A or /EA. ( A ) NGF binds to its receptor TrkA, activates the signalling cascades shown [ 18 ], and induces Ca 2+ influx by an unidentified mechanism ( ? ). Elevated intracellular Ca 2+ ([Ca 2+ ] i ) triggers the fusion of large dense core vesicles (LDCVs) via SNARE-complexes (VAMP, syntaxin-1 and SNAP-25), thereby, causing exocytotic release of CGRP and surface delivery of vesicle constituents. This acute potentiation by NGF can involve the phosphatidylinositol 3-kinase—Src ( PI3K-Src) pathway, which promotes trafficking of LDCVs, and insertion of their TRPV1 channels into the plasmalemma by Ca 2+ -regulated exocytosis (blue arrows) c.f. [ 18 , 21 ]. Additionally, the phospholipase C γ (PLCγ) cascade leads to sensitisation of TRPV1 already on the plasmalemma (red dashed arrows) [ 18 ]. The outcome of these composite influences of NGF on TRPV1 is that when the channel is activated by CAP [Ca 2+ ] I is raised even more than normally [ 15 , 18 , 21 ] and this further enhances CGRP release ( Figure 2 ). ( B ) The proteases of BoNT/A and/EA delete 9 (purple arrow) and 26 (green arrow) residues from SNAP-25 (Insert), respectively, preventing the fusion of LDCVs; this blocks the minimal CGRP exocytosis elicited by NGF (arrow with crosses, left) and its enhancement of the release evoked by 20 nM CAP ( ) (arrow with crosses, centre). Stronger stimulation of TRPV1 with 1 µM CAP ( ) induces a lot more Ca 2+ influx ( [Ca 2+ ] i , ( [Ca 2+ ] i , ( [Ca 2+ ] i ; Figure 1 ) which causes a moderate increase in CGRP release but overcomes the inhibition by BoNT/A (purple cross with broken lines, right) while/EA (green cross, right) remains effective in diminishing CGRP release. Acute sensitisation by NGF of TRPV1 selectively enhances neuropeptide exocytosis stimulated by low [CAP] (

    Journal: International Journal of Molecular Sciences

    Article Title: NGF Enhances CGRP Release Evoked by Capsaicin from Rat Trigeminal Neurons: Differential Inhibition by SNAP-25-Cleaving Proteases

    doi: 10.3390/ijms23020892

    Figure Lengend Snippet: NGF induces a minor increase in Ca 2+ - and SNARE-dependent CGRP release, whereas it greatly enhances the CAP-evoked exocytosis which is blocked by BoNT/A and/EA at low [CAP] but only abolished by BoNT/EA at higher [CAP]. ( A ) Illustrates the effect of acute NGF on CGRP exocytosis from control neonatal rat TGNs starved of the neurotrophin for 2 days, and ( B ) in TGNs pre-treated with BoNT/A or /EA. ( A ) NGF binds to its receptor TrkA, activates the signalling cascades shown [ 18 ], and induces Ca 2+ influx by an unidentified mechanism ( ? ). Elevated intracellular Ca 2+ ([Ca 2+ ] i ) triggers the fusion of large dense core vesicles (LDCVs) via SNARE-complexes (VAMP, syntaxin-1 and SNAP-25), thereby, causing exocytotic release of CGRP and surface delivery of vesicle constituents. This acute potentiation by NGF can involve the phosphatidylinositol 3-kinase—Src ( PI3K-Src) pathway, which promotes trafficking of LDCVs, and insertion of their TRPV1 channels into the plasmalemma by Ca 2+ -regulated exocytosis (blue arrows) c.f. [ 18 , 21 ]. Additionally, the phospholipase C γ (PLCγ) cascade leads to sensitisation of TRPV1 already on the plasmalemma (red dashed arrows) [ 18 ]. The outcome of these composite influences of NGF on TRPV1 is that when the channel is activated by CAP [Ca 2+ ] I is raised even more than normally [ 15 , 18 , 21 ] and this further enhances CGRP release ( Figure 2 ). ( B ) The proteases of BoNT/A and/EA delete 9 (purple arrow) and 26 (green arrow) residues from SNAP-25 (Insert), respectively, preventing the fusion of LDCVs; this blocks the minimal CGRP exocytosis elicited by NGF (arrow with crosses, left) and its enhancement of the release evoked by 20 nM CAP ( ) (arrow with crosses, centre). Stronger stimulation of TRPV1 with 1 µM CAP ( ) induces a lot more Ca 2+ influx ( [Ca 2+ ] i , ( [Ca 2+ ] i , ( [Ca 2+ ] i ; Figure 1 ) which causes a moderate increase in CGRP release but overcomes the inhibition by BoNT/A (purple cross with broken lines, right) while/EA (green cross, right) remains effective in diminishing CGRP release. Acute sensitisation by NGF of TRPV1 selectively enhances neuropeptide exocytosis stimulated by low [CAP] (

    Article Snippet: NGF 2.5S and antibodies to NGF (AN-240) and TRPV1 (ACC-030; used to determine TRPV1 expression by Western blotting) were supplied by Alomone Labs (Jerusalem, Israel).

    Techniques: Inhibition

    IAN transection both in NP and non-NP groups changes the expression profile of TRPV1 to myelinated neurons of a larger diameter. The total number of TG cells labeled for the fluoro-gold (FG + ) (A: NP group, B: Non-NP group); TG cells that labeled for TRPV1 and FG (TRPV1 + +FG + ) in 2-; 3-and 4-week NP groups and in sham-operated group (C: NP group, D: Non-NP group). The ratio of TRPV1 + +FG + to all FG+ positive cells (E: NP group, F: Non-NP group). n = 5 for each group, (ANOVA followed by the Student–Newman–Keuls test, *p

    Journal: PLoS ONE

    Article Title: Expression of TRPV1 Channels after Nerve Injury Provides an Essential Delivery Tool for Neuropathic Pain Attenuation

    doi: 10.1371/journal.pone.0044023

    Figure Lengend Snippet: IAN transection both in NP and non-NP groups changes the expression profile of TRPV1 to myelinated neurons of a larger diameter. The total number of TG cells labeled for the fluoro-gold (FG + ) (A: NP group, B: Non-NP group); TG cells that labeled for TRPV1 and FG (TRPV1 + +FG + ) in 2-; 3-and 4-week NP groups and in sham-operated group (C: NP group, D: Non-NP group). The ratio of TRPV1 + +FG + to all FG+ positive cells (E: NP group, F: Non-NP group). n = 5 for each group, (ANOVA followed by the Student–Newman–Keuls test, *p

    Article Snippet: They were then coincubated overnight at 4°C with a combination of rabbit anti-TRPV1 antibody (1∶200; Alomone Labs Ltd., Israel), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100, and mouse monoclonal anti-neurofilament 200 (NF200) antibody (1∶1000; Sigma-Aldrich), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100.

    Techniques: Expressing, Labeling

    Photomicrographs of immunohistochemistry of TG cells labeled for TRPV1, NF200 and FG in sham-operated group and in 2-; 3-and 4-week NP groups and in 2-; 3- and 4 weeks non-NP groups. Expanded view of TG in the sham-operated group (D1–D4). Arrow points on an example of TRPV1 + +FG + +NF - cell. Arrowhead points on an example of TRPV1 + +FG + +NF + cell. Note that TRPV1-positive cells increased with time after transection. Scale bar: 50 µm.

    Journal: PLoS ONE

    Article Title: Expression of TRPV1 Channels after Nerve Injury Provides an Essential Delivery Tool for Neuropathic Pain Attenuation

    doi: 10.1371/journal.pone.0044023

    Figure Lengend Snippet: Photomicrographs of immunohistochemistry of TG cells labeled for TRPV1, NF200 and FG in sham-operated group and in 2-; 3-and 4-week NP groups and in 2-; 3- and 4 weeks non-NP groups. Expanded view of TG in the sham-operated group (D1–D4). Arrow points on an example of TRPV1 + +FG + +NF - cell. Arrowhead points on an example of TRPV1 + +FG + +NF + cell. Note that TRPV1-positive cells increased with time after transection. Scale bar: 50 µm.

    Article Snippet: They were then coincubated overnight at 4°C with a combination of rabbit anti-TRPV1 antibody (1∶200; Alomone Labs Ltd., Israel), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100, and mouse monoclonal anti-neurofilament 200 (NF200) antibody (1∶1000; Sigma-Aldrich), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100.

    Techniques: Immunohistochemistry, Labeling

    The pattern of distribution of TRPV1 was altered in non-NP groups. The distribution area of TRPV1 + +FG + +NF + positive cells for all experimental groups. A cell area > 1000 µm 2 was considered large, while that

    Journal: PLoS ONE

    Article Title: Expression of TRPV1 Channels after Nerve Injury Provides an Essential Delivery Tool for Neuropathic Pain Attenuation

    doi: 10.1371/journal.pone.0044023

    Figure Lengend Snippet: The pattern of distribution of TRPV1 was altered in non-NP groups. The distribution area of TRPV1 + +FG + +NF + positive cells for all experimental groups. A cell area > 1000 µm 2 was considered large, while that

    Article Snippet: They were then coincubated overnight at 4°C with a combination of rabbit anti-TRPV1 antibody (1∶200; Alomone Labs Ltd., Israel), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100, and mouse monoclonal anti-neurofilament 200 (NF200) antibody (1∶1000; Sigma-Aldrich), which was diluted with 3% NGS in 0.01 M PBS with 0.3% Triton X-100.

    Techniques: