anti rat igg whole molecule  (Millipore)


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  • 96
    Name:
    Anti Rat IgG
    Description:

    Catalog Number:
    sab4600017
    Price:
    None
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    Structured Review

    Millipore anti rat igg whole molecule
    Anti Rat IgG

    https://www.bioz.com/result/anti rat igg whole molecule/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rat igg whole molecule - by Bioz Stars, 2021-01
    96/100 stars

    Related Products / Commonly Used Together

    pbs
    fluorescein isothiocyanate

    Images

    Related Articles

    Microscopy:

    Article Title: The effects of N-terminal insertion into VSV-G of an scFv peptide
    Article Snippet: .. The proteins were visualised with a rat anti-HA antibody together with a FITC labelled anti-Rat IgG (both Sigma), and analysed with a confocal microscope (Leica). .. Detection of scFv/VSV-G by flow cytometry 2 × 105 transfected 293T cells were collected in phosphate buffered saline (PBS), incubated in block buffer (BB: 10% bovines serum albumin, 0.1 M Glycine in PBS) for 30 minutes on ice, which was replaced by 200 μl of 5G8F11 hybridoma supernatant.

    Purification:

    Article Title: Interleukin-12 Promotes Gamma Interferon-Dependent Neutrophil Recruitment in the Lung and Improves Protection against Respiratory Streptococcus pneumoniae Infection ▿
    Article Snippet: .. Anti-Ly6G MAb was purified from culture supernatants of the RB6-8C5 hybridoma using anti-rat IgG agarose columns (Sigma). .. Wild-type BALB/c mice were injected intraperitoneally with 0.5 mg of RB6-8C5 anti-Ly6G MAb or rat IgG as a control on days −1 and 0 of infection to deplete neutrophils.

    Article Title: A Detrimental Effect of Interleukin-10 on Protective Pulmonary Humoral Immunity during Primary Influenza A Virus Infection ▿
    Article Snippet: .. Anti-Ly6G MAb was purified from culture supernatants of RB6-8C5 hybridomas by use of anti-rat IgG agarose columns (Sigma). .. Mice were injected intraperitoneally (i.p.) with 100 μg of MAb RB6-8C5 daily for 2 days before influenza infection, followed by inoculation every 3 days after infection (three to five mice/group).

    Incubation:

    Article Title: High-molecular-weight hyaluronan is a novel inhibitor of pulmonary vascular leakiness
    Article Snippet: .. Human pulmonary microvascular EC were plated on 8-well glass cover slides and allowed to adhere for 48 h. Cells were then serum-starved for 1 h and incubated with 100 nM HMW-HA for 15 min and fixed in 4% paraformaldehyde or incubated with rat anti-CD44 (IM-7) antibody at 10 μg/ml for 2 h. Cells were then washed briefly in PBS, pH 7.4, and secondary anti-rat IgG (1 μg/ml; Sigma-Aldrich) was added for CD44 antibody cross-linking ( .. CEM are dynamic structures that can redistribute in EC under certain conditions, including cytokine stimulation, laminar flow, and migration ( , ).

    Article Title: Peripheral blood fibrocytes: new information to explain the dynamics of Leishmania infection
    Article Snippet: .. The cells were then incubated with a rat anti-CD45 IgG2a monoclonal primary antibody (Santa Cruz Biotechnology, Inc, Heidelbergh, Germany) and an anti-rat IgG fluorescein isothiocyanate (FITC)-conjugated secondary antibody (Sigma-Aldrich). .. The cells were fixed in 1% paraformaldehyde (PFA), permeabilised with 0.2% saponin (Sigma-Aldrich) and incubated with a rabbit anti-heat shock protein (HSP)47 primary antibody (Santa Cruz Biotechnology).

    Article Title: Transgenic Mice With Enhanced Neuronal Major Histocompatibility Complex Class I Expression Recover Locomotor Function Better After Spinal Cord Injury
    Article Snippet: .. After blocking of nonspecific binding, endogenous peroxidase activity, and biotin, sections were incubated with rat ER-HR52 (2 μg/ml) or rat IgG2a isotype control (eBR2a, 2 μg/ml; eBioscience), followed by biotinylated anti-rat IgG (2 μg/ml; Sigma, St. Louis, MO) and a streptoavidin-HRP detection system (Dako, Carpinteria, CA). .. Images were taken using a Nikon microscope (Microphot-FXA) with a ×20 objective and a Spot digital camera (1400Color).

    Article Title: Habituation of Bean (Phaseolus vulgaris) Cell Cultures to Quinclorac and Analysis of the Subsequent Cell Wall Modifications
    Article Snippet: .. After exhaustive washes with PBS, the sections were incubated in darkness for 2 h with a 1/100 dilution of an anti-rat immunoglobulin G linked to fluorescein isothiocyanate (FITC; Sigma) in MPBS at room temperature. .. Finally, the sections were washed with PBS and mounted in a glycerol/PBS-based antifade solution (Citifluor AF1; Agar Scientific, London, UK).

    Activity Assay:

    Article Title: Transgenic Mice With Enhanced Neuronal Major Histocompatibility Complex Class I Expression Recover Locomotor Function Better After Spinal Cord Injury
    Article Snippet: .. After blocking of nonspecific binding, endogenous peroxidase activity, and biotin, sections were incubated with rat ER-HR52 (2 μg/ml) or rat IgG2a isotype control (eBR2a, 2 μg/ml; eBioscience), followed by biotinylated anti-rat IgG (2 μg/ml; Sigma, St. Louis, MO) and a streptoavidin-HRP detection system (Dako, Carpinteria, CA). .. Images were taken using a Nikon microscope (Microphot-FXA) with a ×20 objective and a Spot digital camera (1400Color).

    Blocking Assay:

    Article Title: Transgenic Mice With Enhanced Neuronal Major Histocompatibility Complex Class I Expression Recover Locomotor Function Better After Spinal Cord Injury
    Article Snippet: .. After blocking of nonspecific binding, endogenous peroxidase activity, and biotin, sections were incubated with rat ER-HR52 (2 μg/ml) or rat IgG2a isotype control (eBR2a, 2 μg/ml; eBioscience), followed by biotinylated anti-rat IgG (2 μg/ml; Sigma, St. Louis, MO) and a streptoavidin-HRP detection system (Dako, Carpinteria, CA). .. Images were taken using a Nikon microscope (Microphot-FXA) with a ×20 objective and a Spot digital camera (1400Color).

    Binding Assay:

    Article Title: Transgenic Mice With Enhanced Neuronal Major Histocompatibility Complex Class I Expression Recover Locomotor Function Better After Spinal Cord Injury
    Article Snippet: .. After blocking of nonspecific binding, endogenous peroxidase activity, and biotin, sections were incubated with rat ER-HR52 (2 μg/ml) or rat IgG2a isotype control (eBR2a, 2 μg/ml; eBioscience), followed by biotinylated anti-rat IgG (2 μg/ml; Sigma, St. Louis, MO) and a streptoavidin-HRP detection system (Dako, Carpinteria, CA). .. Images were taken using a Nikon microscope (Microphot-FXA) with a ×20 objective and a Spot digital camera (1400Color).

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  • 97
    Millipore rat anti mouse igg1
    Serum titers of IgG, IgM, <t>IgG1</t> and IgG2a antibodies. A . ELISA analysis of titers of IgG, IgM, and specific anti-AGE-LDL IgG and IgM antibodies in sera from diabetic apoE−/− and LDLR−/− mice (n = 10 per group) treated with Alum adjuvant (bar with stripes), AGE-LDL + Alum adjuvant (black bar) or PBS controls (white bar). B . ELISA analysis of specific IgG1 and IgG2a antibodies against AGE-LDL in sera from diabetic apoE−/− and LDLR−/− mice treated with Alum adjuvant, AGE-LDL + Alum adjuvant or PBS controls. C shows the ratio of serum IgG1/IgG2a antibodies among three groups. *indicates p
    Rat Anti Mouse Igg1, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti mouse igg1/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rat anti mouse igg1 - by Bioz Stars, 2021-01
    97/100 stars
      Buy from Supplier

    99
    Millipore fitc conjugated anti rabbit igg
    NMI inhibits the nuclear translocation of NF-κB/p65 after TNF-α stimulation. (A) HeLa cells were transfected with control or Myc-NMI plasmids, and at 24 h posttransfection, the cells were left untreated or treated with TNF-α (10 ng/ml) for 60 min. The cells were fixed and incubated with anti-p65 or anti-Myc Ab and then stained with rhodamine-conjugated anti-mouse <t>IgG</t> (red) or <t>FITC-conjugated</t> anti-rabbit Ab (green). The same slide was also stained with DAPI to show the nucleus. The expression and localization of p65 and Myc-NMI was determined by confocal immunofluorescence analysis. The percentage of the cells expressing p65 in the nucleus among the cells that express Myc-NMI and the cells that don’t express Myc-NMI was calculated in the bottom panel. (B) HeLa cells were transfected with control or Myc-NMI plasmids, and at 24 h posttransfection, the cells were left untreated or treated with TNF-α (10 ng/ml) for 60 min. The cells were then harvested and fractionated into the nuclear and cytoplasmic fractions, and the fractions were immunoblotted with anti-NMI and anti-p65 Abs. The quantification by densitometry of western blots of p65 bands without or with Myc-NMI in TNF-α-stimulated nuclear fractions was shown in the right panel. The results are representative of three independent experiments, and the error bars represent the SD.**p
    Fitc Conjugated Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated anti rabbit igg/product/Millipore
    Average 99 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated anti rabbit igg - by Bioz Stars, 2021-01
    99/100 stars
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    97
    Millipore whole immunoglobulin g igg
    Taenia solium oncospheres obtained from a tapeworm carrier are seen under contrast phase microscopy, PI, FITC and a merged image of both stains . The first row corresponds to oncospheres incubated with anti GST <t>IgG</t> as a negative control; rows 2 and 3 include images obtained with the specific anti TSOL18 and anti TSOL45-1A antibodies, respectively. The arrows in the first column point to the hooks of the oncospheres that was used for focus. Positive reactions were detected with anti-TSOL18 and anti-TSOL45-1A antibodies only on the surface of the oncospheres, as seen in the merged image. The high intensity emission of PI in FITC sections caused non-specific background fluorescence on the same internal structures seen in PI sections.
    Whole Immunoglobulin G Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole immunoglobulin g igg/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    whole immunoglobulin g igg - by Bioz Stars, 2021-01
    97/100 stars
      Buy from Supplier

    Image Search Results


    Serum titers of IgG, IgM, IgG1 and IgG2a antibodies. A . ELISA analysis of titers of IgG, IgM, and specific anti-AGE-LDL IgG and IgM antibodies in sera from diabetic apoE−/− and LDLR−/− mice (n = 10 per group) treated with Alum adjuvant (bar with stripes), AGE-LDL + Alum adjuvant (black bar) or PBS controls (white bar). B . ELISA analysis of specific IgG1 and IgG2a antibodies against AGE-LDL in sera from diabetic apoE−/− and LDLR−/− mice treated with Alum adjuvant, AGE-LDL + Alum adjuvant or PBS controls. C shows the ratio of serum IgG1/IgG2a antibodies among three groups. *indicates p

    Journal: Cardiovascular Diabetology

    Article Title: Immunization with advanced glycation end products modified low density lipoprotein inhibits atherosclerosis progression in diabetic apoE and LDLR null mice

    doi: 10.1186/s12933-014-0151-6

    Figure Lengend Snippet: Serum titers of IgG, IgM, IgG1 and IgG2a antibodies. A . ELISA analysis of titers of IgG, IgM, and specific anti-AGE-LDL IgG and IgM antibodies in sera from diabetic apoE−/− and LDLR−/− mice (n = 10 per group) treated with Alum adjuvant (bar with stripes), AGE-LDL + Alum adjuvant (black bar) or PBS controls (white bar). B . ELISA analysis of specific IgG1 and IgG2a antibodies against AGE-LDL in sera from diabetic apoE−/− and LDLR−/− mice treated with Alum adjuvant, AGE-LDL + Alum adjuvant or PBS controls. C shows the ratio of serum IgG1/IgG2a antibodies among three groups. *indicates p

    Article Snippet: After washing, bound antibodies were detected by using biotinylated goat anti-mouse IgM (AP500B, Millipore, USA) or IgG (B7264, Sigma, USA) or rat anti-mouse IgG1 or IgG2a secondary antibodies (RMG115 and RMG2a15, Millipore, USA) that were incubated for 2 hours at room temperature.

    Techniques: Enzyme-linked Immunosorbent Assay, Mouse Assay

    NMI inhibits the nuclear translocation of NF-κB/p65 after TNF-α stimulation. (A) HeLa cells were transfected with control or Myc-NMI plasmids, and at 24 h posttransfection, the cells were left untreated or treated with TNF-α (10 ng/ml) for 60 min. The cells were fixed and incubated with anti-p65 or anti-Myc Ab and then stained with rhodamine-conjugated anti-mouse IgG (red) or FITC-conjugated anti-rabbit Ab (green). The same slide was also stained with DAPI to show the nucleus. The expression and localization of p65 and Myc-NMI was determined by confocal immunofluorescence analysis. The percentage of the cells expressing p65 in the nucleus among the cells that express Myc-NMI and the cells that don’t express Myc-NMI was calculated in the bottom panel. (B) HeLa cells were transfected with control or Myc-NMI plasmids, and at 24 h posttransfection, the cells were left untreated or treated with TNF-α (10 ng/ml) for 60 min. The cells were then harvested and fractionated into the nuclear and cytoplasmic fractions, and the fractions were immunoblotted with anti-NMI and anti-p65 Abs. The quantification by densitometry of western blots of p65 bands without or with Myc-NMI in TNF-α-stimulated nuclear fractions was shown in the right panel. The results are representative of three independent experiments, and the error bars represent the SD.**p

    Journal: Scientific Reports

    Article Title: N-Myc-Interacting Protein Negatively Regulates TNF-α-Induced NF-κB Transcriptional Activity by Sequestering NF-κB/p65 in the Cytoplasm

    doi: 10.1038/s41598-017-15074-5

    Figure Lengend Snippet: NMI inhibits the nuclear translocation of NF-κB/p65 after TNF-α stimulation. (A) HeLa cells were transfected with control or Myc-NMI plasmids, and at 24 h posttransfection, the cells were left untreated or treated with TNF-α (10 ng/ml) for 60 min. The cells were fixed and incubated with anti-p65 or anti-Myc Ab and then stained with rhodamine-conjugated anti-mouse IgG (red) or FITC-conjugated anti-rabbit Ab (green). The same slide was also stained with DAPI to show the nucleus. The expression and localization of p65 and Myc-NMI was determined by confocal immunofluorescence analysis. The percentage of the cells expressing p65 in the nucleus among the cells that express Myc-NMI and the cells that don’t express Myc-NMI was calculated in the bottom panel. (B) HeLa cells were transfected with control or Myc-NMI plasmids, and at 24 h posttransfection, the cells were left untreated or treated with TNF-α (10 ng/ml) for 60 min. The cells were then harvested and fractionated into the nuclear and cytoplasmic fractions, and the fractions were immunoblotted with anti-NMI and anti-p65 Abs. The quantification by densitometry of western blots of p65 bands without or with Myc-NMI in TNF-α-stimulated nuclear fractions was shown in the right panel. The results are representative of three independent experiments, and the error bars represent the SD.**p

    Article Snippet: Immunocomplexes were resolved by SDS-PAGE, and Western blotting was performed with the following Abs: anti-FLAG M2 and anti-actin monoclonal Abs (Sigma-Aldrich); anti-HA (Y-11), anti-IκB (C-20) and anti-NMI (N-16) polyclonal Abs, anti-Myc (9E10), anti-GFP (B-2) mAb (Santa Cruz Biotechnology); NF-κB/p65 (L8F6) mAb (Cell signaling); horseradish peroxidade-conjugated goat anti-mouse, goat anti-rabbit and donkey-anti goat (Thermo Scientific, Waltham, MA), and FITC-conjugated anti-rabbit IgG or rhodamine-conjugated anti-mouse IgG (Millipore).

    Techniques: Translocation Assay, Transfection, Incubation, Staining, Expressing, Immunofluorescence, Western Blot

    Effect of MIR exposure on DNA double strain breaks in A549 cells. Cells were seeded onto the glass coverslip in 12-well plate, exposure by MIR for 48 hours in the presence or absence of 10 mM N-Acetyl-Cysteine (NAC). Cells were treated with NAC for 1 h prior to MIR exposure (NAC 1 h) or cotreated throughout the exposure for 48 h (NAC). Cells were fixed for staining and visualized by fluorescence microscopy. 53BP1 was labeled with rabbit anti-53BP1 antibody and corresponded FITC–conjugated anti-rabbit IgG antibody (green), γ-H2AX was labeled with mouse anti-γ-H2AX antibody following corresponded PE–conjugated anti-mouse IgG antibody (red), and nuclei were labeled with DAPI (blue). Scale bar represents 10 µm.

    Journal: PLoS ONE

    Article Title: Middle Infrared Radiation Induces G2/M Cell Cycle Arrest in A549 Lung Cancer Cells

    doi: 10.1371/journal.pone.0054117

    Figure Lengend Snippet: Effect of MIR exposure on DNA double strain breaks in A549 cells. Cells were seeded onto the glass coverslip in 12-well plate, exposure by MIR for 48 hours in the presence or absence of 10 mM N-Acetyl-Cysteine (NAC). Cells were treated with NAC for 1 h prior to MIR exposure (NAC 1 h) or cotreated throughout the exposure for 48 h (NAC). Cells were fixed for staining and visualized by fluorescence microscopy. 53BP1 was labeled with rabbit anti-53BP1 antibody and corresponded FITC–conjugated anti-rabbit IgG antibody (green), γ-H2AX was labeled with mouse anti-γ-H2AX antibody following corresponded PE–conjugated anti-mouse IgG antibody (red), and nuclei were labeled with DAPI (blue). Scale bar represents 10 µm.

    Article Snippet: After washed with PBST (PBS containing 0.05% Tween-20, Sigma-Aldrich, St Louis, MO, USA) three times, cells were labeled with corresponding secondary anti-mouse IgG-Alexa 488 (Invitrogen, Carlsbad, CA; 1∶1000), anti-mouse IgG-PE (Abcam; 1∶1000), or secondary anti-rabbit FITC-IgG (Millipore; 1∶200) for 1 hour in the dark at room temperature.

    Techniques: Staining, Fluorescence, Microscopy, Labeling

    Effect of MIR exposure on the actin filaments and focal adhesions of A549 cells. Cells were seeded onto glass coverslips in 12-well plates, exposed to MIR for 48 hours, fixed for staining and visualized by fluorescence microscopy. Actin filaments were tagged with rhodamine-labeled phalloidin (red), vinculin was labeled with mouse anti-vinculin antibody and the corresponding FITC– conjugated secondary anti-mouse IgG antibody (green), and nuclei were stained with DAPI (blue). Scale bar represents 10 µm. Arrows indicate the position of vinculin.

    Journal: PLoS ONE

    Article Title: Middle Infrared Radiation Induces G2/M Cell Cycle Arrest in A549 Lung Cancer Cells

    doi: 10.1371/journal.pone.0054117

    Figure Lengend Snippet: Effect of MIR exposure on the actin filaments and focal adhesions of A549 cells. Cells were seeded onto glass coverslips in 12-well plates, exposed to MIR for 48 hours, fixed for staining and visualized by fluorescence microscopy. Actin filaments were tagged with rhodamine-labeled phalloidin (red), vinculin was labeled with mouse anti-vinculin antibody and the corresponding FITC– conjugated secondary anti-mouse IgG antibody (green), and nuclei were stained with DAPI (blue). Scale bar represents 10 µm. Arrows indicate the position of vinculin.

    Article Snippet: After washed with PBST (PBS containing 0.05% Tween-20, Sigma-Aldrich, St Louis, MO, USA) three times, cells were labeled with corresponding secondary anti-mouse IgG-Alexa 488 (Invitrogen, Carlsbad, CA; 1∶1000), anti-mouse IgG-PE (Abcam; 1∶1000), or secondary anti-rabbit FITC-IgG (Millipore; 1∶200) for 1 hour in the dark at room temperature.

    Techniques: Staining, Fluorescence, Microscopy, Labeling

    Taenia solium oncospheres obtained from a tapeworm carrier are seen under contrast phase microscopy, PI, FITC and a merged image of both stains . The first row corresponds to oncospheres incubated with anti GST IgG as a negative control; rows 2 and 3 include images obtained with the specific anti TSOL18 and anti TSOL45-1A antibodies, respectively. The arrows in the first column point to the hooks of the oncospheres that was used for focus. Positive reactions were detected with anti-TSOL18 and anti-TSOL45-1A antibodies only on the surface of the oncospheres, as seen in the merged image. The high intensity emission of PI in FITC sections caused non-specific background fluorescence on the same internal structures seen in PI sections.

    Journal: Parasites & Vectors

    Article Title: Immunolocalization of TSOL18 and TSOL45-1A, the successful protective peptides against porcine cysticercosis, in Taenia solium oncospheres

    doi: 10.1186/1756-3305-4-3

    Figure Lengend Snippet: Taenia solium oncospheres obtained from a tapeworm carrier are seen under contrast phase microscopy, PI, FITC and a merged image of both stains . The first row corresponds to oncospheres incubated with anti GST IgG as a negative control; rows 2 and 3 include images obtained with the specific anti TSOL18 and anti TSOL45-1A antibodies, respectively. The arrows in the first column point to the hooks of the oncospheres that was used for focus. Positive reactions were detected with anti-TSOL18 and anti-TSOL45-1A antibodies only on the surface of the oncospheres, as seen in the merged image. The high intensity emission of PI in FITC sections caused non-specific background fluorescence on the same internal structures seen in PI sections.

    Article Snippet: Whole immunoglobulin G (IgG) was purified with a commercial kit (Montage Antibody Purification Prosep-A, Millipore, Bedford, MA, USA), the purified IgG was adjusted at 1 mg/ml.

    Techniques: Microscopy, Incubation, Negative Control, Fluorescence