Journal: Cells

Article Title: Src Family Kinases Facilitate the Crosstalk between CGRP and Cytokines in Sensitizing Trigeminal Ganglion via Transmitting CGRP Receptor/PKA Pathway

doi: 10.3390/cells11213498

Figure Lengend Snippet: SFKs co-localized with both CGRP and RAMP1 in the mouse TG. ( A ) The representative images of double staining with the anti-CGRP antibody and anti-SFKs antibody in the TG. ( B ) The representative images of double staining with the anti-RAMP1 antibody and anti-SFKs antibody in the TG. CGRP and RAMP1 were stained with the anti-CGRP antibody and anti-RAMP1 antibody, respectively, and are shown in red; SFKs were stained with the anti-SFKs antibody and are shown in green; nucleus was stained with DAPI and is shown in blue. Co-localization of SFKs and CGRP or RAMP1 is shown in yellow and is indicated by the white arrows.

Article Snippet: Subsequently, the TG slice was incubated with anti-SFKs antibody (1:40, AF3389, R&D Systems, Minneapolis, MN, USA) with anti-CGRP antibody (1:50, ab81887, Abcam, Cambridge, UK) or anti-RAMP1 antibody (1:50, ARR-021, Alomone Labs, Jerusalem, Israel) respectively at 4 °C overnight.

Techniques: Double Staining, Staining