anti rabbit  (Millipore)


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  • 99
    Name:
    IgM Rabbit Polyclonal Antibody
    Description:
    Anti IgM antibody reacts with surface immunoglobulin IgM mu chains IgM is one of the predominant surface immunoglobulins on B lymphocytes This antibody is useful when identifying lymphomas plasmacytomas and B cell lineage derived Hodgkin s lymphomas Due to the restricted expression of heavy and light chains in these diseases demonstration of B cell lymphomas is possible with clonal gene rearrangement studies
    Catalog Number:
    270a-1
    Price:
    None
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    Structured Review

    Millipore anti rabbit
    IgM Rabbit Polyclonal Antibody
    Anti IgM antibody reacts with surface immunoglobulin IgM mu chains IgM is one of the predominant surface immunoglobulins on B lymphocytes This antibody is useful when identifying lymphomas plasmacytomas and B cell lineage derived Hodgkin s lymphomas Due to the restricted expression of heavy and light chains in these diseases demonstration of B cell lymphomas is possible with clonal gene rearrangement studies
    https://www.bioz.com/result/anti rabbit/product/Millipore
    Average 99 stars, based on 430 article reviews
    Price from $9.99 to $1999.99
    anti rabbit - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
    Article Snippet: .. Analysis of bacterial protein A surface expression Bacteria were washed in PBS and incubated for 1 h at 25°C with polyclonal FITC-conjugated rabbit-anti-goat immunoglobulin G (H+L, Sigma, Germany) for flow cytometry or polyclonal rabbit antibody directed against ovalbumin (C6534, Sigma, Germany) for immunofluorescence microscopy. .. Controls were incubated with PBS.

    Article Title: Expression and Activation by Epstein Barr Virus of Human Endogenous Retroviruses-W in Blood Cells and Astrocytes: Inference for Multiple Sclerosis
    Article Snippet: .. Flow cytometry The detection of HERV-Wenv protein on the plasma membrane was performed using anti-HERV-Wenv rabbit polyclonal antibody (Allele Biotec) and secondary fluorescein isothiocyanate-conjugated goat anti-rabbit IgG (Sigma-Aldrich). .. The isotype control was a pre-immune rabbit serum (Santa Cruz Biotechnology, Inc).

    Cytometry:

    Article Title: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
    Article Snippet: .. Analysis of bacterial protein A surface expression Bacteria were washed in PBS and incubated for 1 h at 25°C with polyclonal FITC-conjugated rabbit-anti-goat immunoglobulin G (H+L, Sigma, Germany) for flow cytometry or polyclonal rabbit antibody directed against ovalbumin (C6534, Sigma, Germany) for immunofluorescence microscopy. .. Controls were incubated with PBS.

    Article Title: Expression and Activation by Epstein Barr Virus of Human Endogenous Retroviruses-W in Blood Cells and Astrocytes: Inference for Multiple Sclerosis
    Article Snippet: .. Flow cytometry The detection of HERV-Wenv protein on the plasma membrane was performed using anti-HERV-Wenv rabbit polyclonal antibody (Allele Biotec) and secondary fluorescein isothiocyanate-conjugated goat anti-rabbit IgG (Sigma-Aldrich). .. The isotype control was a pre-immune rabbit serum (Santa Cruz Biotechnology, Inc).

    Microscopy:

    Article Title: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
    Article Snippet: .. Analysis of bacterial protein A surface expression Bacteria were washed in PBS and incubated for 1 h at 25°C with polyclonal FITC-conjugated rabbit-anti-goat immunoglobulin G (H+L, Sigma, Germany) for flow cytometry or polyclonal rabbit antibody directed against ovalbumin (C6534, Sigma, Germany) for immunofluorescence microscopy. .. Controls were incubated with PBS.

    Article Title: Identification of conserved, centrosome-targeting ASH domains in TRAPPII complex subunits and TRAPPC8
    Article Snippet: .. Cells were fixed with methanol and subjected to immunofluorescence microscopy as described [ ] using rabbit polyclonal antibody specific for TRAPPC8 (1:100 dilution; Sigma), rat monoclonal antibody specific for EB3 (1:300 dilution; Absea clone KT36), and mouse monoclonal antibodies specific for acetylated-tubulin (1:5,000 dilution; Sigma) and p150Glued (1:500 dilution; BD Biosciences). .. Imaging was performed with a motorized Olympus BX63 upright microscope equipped with a DP72 color, 12.8 megapixel, 4140 × 3096 resolution camera and differential interference contrast (DIC).

    Incubation:

    Article Title: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
    Article Snippet: .. Analysis of bacterial protein A surface expression Bacteria were washed in PBS and incubated for 1 h at 25°C with polyclonal FITC-conjugated rabbit-anti-goat immunoglobulin G (H+L, Sigma, Germany) for flow cytometry or polyclonal rabbit antibody directed against ovalbumin (C6534, Sigma, Germany) for immunofluorescence microscopy. .. Controls were incubated with PBS.

    Article Title: Identification of peptide domains involved in the subcellular localization of the feline coronavirus 3b protein
    Article Snippet: .. The blots were further incubated overnight with ABfinity recombinant rabbit GFP monoclonal antibodies (Invitrogen) or rabbit anti-FLAG polyclonal antibodies (Sigma-Aldrich) at 4 °C. .. After being washed three times with Tris-buffered saline with 0.1 % (v/v) Tween-20, the blots were incubated for 1 h at RT with HRP-conjugated goat anti-rabbit antibodies (DakoCytomation).

    other:

    Article Title: Laminin 5 Binds the NC-1 Domain of Type VII Collagen
    Article Snippet: Antibodies Preparation and characterization of laminin 5 and 6 antibodies mAb BM165 (anti-α3 chain), mAb SF12 (anti-γ2), mAb 545 (anti-β1), and polyclonal antibody (pAb)1 anti-laminin 5 have been described elsewhere ( ; ). mAb NP32 to the NC-I domain of type VIl procollagen, and pAb made to the whole type VII procollagen molecule have been previously described ( ; ). pAb anti-laminin 1 was purchased from Sigma Chimie (St. Quentin Fallavier, France).

    Expressing:

    Article Title: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
    Article Snippet: .. Analysis of bacterial protein A surface expression Bacteria were washed in PBS and incubated for 1 h at 25°C with polyclonal FITC-conjugated rabbit-anti-goat immunoglobulin G (H+L, Sigma, Germany) for flow cytometry or polyclonal rabbit antibody directed against ovalbumin (C6534, Sigma, Germany) for immunofluorescence microscopy. .. Controls were incubated with PBS.

    Marker:

    Article Title: The Insulin-Like Growth Factor 1 Receptor Is Essential for Axonal Regeneration in Adult Central Nervous System Neurons
    Article Snippet: .. Primary Antibodies The following primary antibodies were used: mouse monoclonal antibody to c-myc (Sigma) diluted 1∶600; mouse monoclonal antibody to the axonal marker Tau-1 (Calbiochem) diluted 1∶600; rabbit polyclonal antibody to βgc 1∶50 for immunofluorescence and 1∶250 for Western blots; rabbit polyclonal antibody to the neurofilament 200 kD sub-unit (NF200) (Sigma) diluted 1∶600 (NF200 is expressed by RGC and horizontal cells in the retina: ); rabbit monoclonal antibody to Phospho-IGF-1 Receptor (tyr980-C14A11-Cell Signaling) diluted 1∶50; goat polyclonal antibody to IGF-1r β subunit (20C sc-713-G, Santa Cruz Biotechnology) diluted 1∶250 for Western blots and a rabbit monoclonal antibody to phospho p85 (tyr458)/p55(Tyr199) (Cell Signaling) diluted 1∶200. .. Cell Culture Retinal cultures were prepared essentially as previously described , , .

    Western Blot:

    Article Title: The Insulin-Like Growth Factor 1 Receptor Is Essential for Axonal Regeneration in Adult Central Nervous System Neurons
    Article Snippet: .. Primary Antibodies The following primary antibodies were used: mouse monoclonal antibody to c-myc (Sigma) diluted 1∶600; mouse monoclonal antibody to the axonal marker Tau-1 (Calbiochem) diluted 1∶600; rabbit polyclonal antibody to βgc 1∶50 for immunofluorescence and 1∶250 for Western blots; rabbit polyclonal antibody to the neurofilament 200 kD sub-unit (NF200) (Sigma) diluted 1∶600 (NF200 is expressed by RGC and horizontal cells in the retina: ); rabbit monoclonal antibody to Phospho-IGF-1 Receptor (tyr980-C14A11-Cell Signaling) diluted 1∶50; goat polyclonal antibody to IGF-1r β subunit (20C sc-713-G, Santa Cruz Biotechnology) diluted 1∶250 for Western blots and a rabbit monoclonal antibody to phospho p85 (tyr458)/p55(Tyr199) (Cell Signaling) diluted 1∶200. .. Cell Culture Retinal cultures were prepared essentially as previously described , , .

    Recombinant:

    Article Title: Identification of peptide domains involved in the subcellular localization of the feline coronavirus 3b protein
    Article Snippet: .. The blots were further incubated overnight with ABfinity recombinant rabbit GFP monoclonal antibodies (Invitrogen) or rabbit anti-FLAG polyclonal antibodies (Sigma-Aldrich) at 4 °C. .. After being washed three times with Tris-buffered saline with 0.1 % (v/v) Tween-20, the blots were incubated for 1 h at RT with HRP-conjugated goat anti-rabbit antibodies (DakoCytomation).

    Immunofluorescence:

    Article Title: Specific antibody-receptor interactions trigger InlAB-independent uptake of listeria monocytogenes into tumor cell lines
    Article Snippet: .. Analysis of bacterial protein A surface expression Bacteria were washed in PBS and incubated for 1 h at 25°C with polyclonal FITC-conjugated rabbit-anti-goat immunoglobulin G (H+L, Sigma, Germany) for flow cytometry or polyclonal rabbit antibody directed against ovalbumin (C6534, Sigma, Germany) for immunofluorescence microscopy. .. Controls were incubated with PBS.

    Article Title: Identification of conserved, centrosome-targeting ASH domains in TRAPPII complex subunits and TRAPPC8
    Article Snippet: .. Cells were fixed with methanol and subjected to immunofluorescence microscopy as described [ ] using rabbit polyclonal antibody specific for TRAPPC8 (1:100 dilution; Sigma), rat monoclonal antibody specific for EB3 (1:300 dilution; Absea clone KT36), and mouse monoclonal antibodies specific for acetylated-tubulin (1:5,000 dilution; Sigma) and p150Glued (1:500 dilution; BD Biosciences). .. Imaging was performed with a motorized Olympus BX63 upright microscope equipped with a DP72 color, 12.8 megapixel, 4140 × 3096 resolution camera and differential interference contrast (DIC).

    Article Title: The Insulin-Like Growth Factor 1 Receptor Is Essential for Axonal Regeneration in Adult Central Nervous System Neurons
    Article Snippet: .. Primary Antibodies The following primary antibodies were used: mouse monoclonal antibody to c-myc (Sigma) diluted 1∶600; mouse monoclonal antibody to the axonal marker Tau-1 (Calbiochem) diluted 1∶600; rabbit polyclonal antibody to βgc 1∶50 for immunofluorescence and 1∶250 for Western blots; rabbit polyclonal antibody to the neurofilament 200 kD sub-unit (NF200) (Sigma) diluted 1∶600 (NF200 is expressed by RGC and horizontal cells in the retina: ); rabbit monoclonal antibody to Phospho-IGF-1 Receptor (tyr980-C14A11-Cell Signaling) diluted 1∶50; goat polyclonal antibody to IGF-1r β subunit (20C sc-713-G, Santa Cruz Biotechnology) diluted 1∶250 for Western blots and a rabbit monoclonal antibody to phospho p85 (tyr458)/p55(Tyr199) (Cell Signaling) diluted 1∶200. .. Cell Culture Retinal cultures were prepared essentially as previously described , , .

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  • 90
    Millipore rabbit anti melanopsin
    Interactions between dendrites of secretoneurin-IR amacrine cells (red) and <t>melanopsin-IR</t> (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.
    Rabbit Anti Melanopsin, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti melanopsin/product/Millipore
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti melanopsin - by Bioz Stars, 2020-07
    90/100 stars
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    85
    Millipore rabbit polyclonal anti e1b 19k
    Formation of tumor giant cells in tumors defective for apoptosis. ( A ) Hematoxylin and eosin-stained sections reveal numerous tumor giant cells in tumors from transformed BMK cells expressing BCL-2 or <t>E1B</t> <t>19K.</t> Typical sections of carcinomas as described in the text are shown at a magnification of 200× and highlight areas enriched for tumor giant cells in tumors from animals injected with transformed BMK cells expressing BCL-2 or E1B 19K, with insets of grossly polyploid cells in mitosis (at 1000× magnification). Several tumor giant cells in each image are indicated by white arrows. Note the absence of tumor giant cells in tumors formed by the W2.3.1–5 cells. A typical section of a tumor area enriched for tumor giant cells was also immunostained for adenovirus E1A to demonstrate that the tumor giant cells are derived from the input-transformed BMK cells. Note the numerous tumor giant cells that stain brown in the E1A immunohistochemistry, including several examples indicated by arrows. A tumor section (20 μm) enriched for tumor giant cells (arrows) was stained with YOYO-1 to reveal DNA content as described in the text. This image is shown at 630×. ( B ) Aberrant metaphases and polyploid cells accumulate during tumor progression. Tumor sections were developed by immunohistochemistry using antibodies specific for phospho-histone H3 and are shown at 200×. Black arrows indicate aberrant polyploid mitotic arrays, and mitotic arrays presented at 600× in the insets are boxed. Top panels represent images of sections from mature tumors. Phosphohistone H3 immunohistochemistry of sections of transformed BMK cell masses excised from mice on days 2 and 9 after injection are shown in the bottom two rows (200×). Insets in the phospho-histone H3 panels were photographed at 600×, and areas present in the insets are boxed. Grossly aberrant mitotic arrays stained for phospho-histone H3 that are evident in the W2.Bcl2–3 and D3.zeo-2, but not W2.3.1–5, cells on day 9 are indicated in the insets by white arrows. Necrotic centers are indicated (N).
    Rabbit Polyclonal Anti E1b 19k, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti e1b 19k/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti e1b 19k - by Bioz Stars, 2020-07
    85/100 stars
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    91
    Millipore rabbit anti nr2a
    Expression of glutamate receptors. (A) Representative western-blot analysis of GluR1, <t>NR2A,</t> and NR2B subunits in the ACC homogenates. Levels of GluR1 (B) , NR2A (C), and NR2B (D) in saline control, vehicle-, T3-, and T4-treated HT mice. n = 5 in each group. * p
    Rabbit Anti Nr2a, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti nr2a/product/Millipore
    Average 91 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    rabbit anti nr2a - by Bioz Stars, 2020-07
    91/100 stars
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    93
    Millipore cy3 conjugated anti rabbit igg
    Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit <t>IgG</t> and a secondary antibody labeled with <t>Cy3.</t> The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.
    Cy3 Conjugated Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated anti rabbit igg/product/Millipore
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated anti rabbit igg - by Bioz Stars, 2020-07
    93/100 stars
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    Image Search Results


    Interactions between dendrites of secretoneurin-IR amacrine cells (red) and melanopsin-IR (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.

    Journal: Peptides

    Article Title: Wavy Multistratified Amacrine Cells in the Monkey Retina Contain Immunoreactive Secretoneurin

    doi: 10.1016/j.peptides.2017.06.005

    Figure Lengend Snippet: Interactions between dendrites of secretoneurin-IR amacrine cells (red) and melanopsin-IR (green) retinal ganglion cells. Secretoneurin-IR dendrites are closely apposed to dendrites of outer-stratifying melanopsin cells (arrowheads). A. Note that a secretoneurin-IR dendrite also contacts the soma of an outer melanopsin cell. The main figure is an orthogonal projection of 6 optical sections showing only the melanopsin signal (green), z step = 0.5 μm, scale bar = 20 μm. Insets are single optical sections displaying both melanopsin (green) and secretoneurin (red) signals, scale bars = 2 μm. B. Note the co-fasciculation of the 2 dendrites. The top figure is an orthogonal projection of 10 optical sections, z step = 0.31 μm. The others are consecutive single optical sections. Scale bar = 5 μm.

    Article Snippet: The first step was 11 days with 1:10,000 rabbit anti-melanopsin, 20% ChemiBLOCKER (Millipore) and 0.3% Triton, and the second was 14 days with 1:10,000 goat anti-secretoneurin in the same diluent.

    Techniques:

    Formation of tumor giant cells in tumors defective for apoptosis. ( A ) Hematoxylin and eosin-stained sections reveal numerous tumor giant cells in tumors from transformed BMK cells expressing BCL-2 or E1B 19K. Typical sections of carcinomas as described in the text are shown at a magnification of 200× and highlight areas enriched for tumor giant cells in tumors from animals injected with transformed BMK cells expressing BCL-2 or E1B 19K, with insets of grossly polyploid cells in mitosis (at 1000× magnification). Several tumor giant cells in each image are indicated by white arrows. Note the absence of tumor giant cells in tumors formed by the W2.3.1–5 cells. A typical section of a tumor area enriched for tumor giant cells was also immunostained for adenovirus E1A to demonstrate that the tumor giant cells are derived from the input-transformed BMK cells. Note the numerous tumor giant cells that stain brown in the E1A immunohistochemistry, including several examples indicated by arrows. A tumor section (20 μm) enriched for tumor giant cells (arrows) was stained with YOYO-1 to reveal DNA content as described in the text. This image is shown at 630×. ( B ) Aberrant metaphases and polyploid cells accumulate during tumor progression. Tumor sections were developed by immunohistochemistry using antibodies specific for phospho-histone H3 and are shown at 200×. Black arrows indicate aberrant polyploid mitotic arrays, and mitotic arrays presented at 600× in the insets are boxed. Top panels represent images of sections from mature tumors. Phosphohistone H3 immunohistochemistry of sections of transformed BMK cell masses excised from mice on days 2 and 9 after injection are shown in the bottom two rows (200×). Insets in the phospho-histone H3 panels were photographed at 600×, and areas present in the insets are boxed. Grossly aberrant mitotic arrays stained for phospho-histone H3 that are evident in the W2.Bcl2–3 and D3.zeo-2, but not W2.3.1–5, cells on day 9 are indicated in the insets by white arrows. Necrotic centers are indicated (N).

    Journal: Genes & Development

    Article Title: Hypoxia and defective apoptosis drive genomic instability and tumorigenesis

    doi: 10.1101/gad.1204904

    Figure Lengend Snippet: Formation of tumor giant cells in tumors defective for apoptosis. ( A ) Hematoxylin and eosin-stained sections reveal numerous tumor giant cells in tumors from transformed BMK cells expressing BCL-2 or E1B 19K. Typical sections of carcinomas as described in the text are shown at a magnification of 200× and highlight areas enriched for tumor giant cells in tumors from animals injected with transformed BMK cells expressing BCL-2 or E1B 19K, with insets of grossly polyploid cells in mitosis (at 1000× magnification). Several tumor giant cells in each image are indicated by white arrows. Note the absence of tumor giant cells in tumors formed by the W2.3.1–5 cells. A typical section of a tumor area enriched for tumor giant cells was also immunostained for adenovirus E1A to demonstrate that the tumor giant cells are derived from the input-transformed BMK cells. Note the numerous tumor giant cells that stain brown in the E1A immunohistochemistry, including several examples indicated by arrows. A tumor section (20 μm) enriched for tumor giant cells (arrows) was stained with YOYO-1 to reveal DNA content as described in the text. This image is shown at 630×. ( B ) Aberrant metaphases and polyploid cells accumulate during tumor progression. Tumor sections were developed by immunohistochemistry using antibodies specific for phospho-histone H3 and are shown at 200×. Black arrows indicate aberrant polyploid mitotic arrays, and mitotic arrays presented at 600× in the insets are boxed. Top panels represent images of sections from mature tumors. Phosphohistone H3 immunohistochemistry of sections of transformed BMK cell masses excised from mice on days 2 and 9 after injection are shown in the bottom two rows (200×). Insets in the phospho-histone H3 panels were photographed at 600×, and areas present in the insets are boxed. Grossly aberrant mitotic arrays stained for phospho-histone H3 that are evident in the W2.Bcl2–3 and D3.zeo-2, but not W2.3.1–5, cells on day 9 are indicated in the insets by white arrows. Necrotic centers are indicated (N).

    Article Snippet: Primary antibodies used were hamster anti-human BCL-2 (BD-Pharmingen); rabbit polyclonal anti-E1B 19K , mouse anti-actin (Oncogene Research Products), rabbit anti-mouse HIF-1α (Cayman), rabbit anti-BIM (Axxora), or rabbit polyclonal antisera raised in our lab against a GST-human PUMA fusion protein encoding a 102-amino acid region common to PUMA-α and PUMA-β.

    Techniques: Staining, Transformation Assay, Expressing, Injection, Derivative Assay, Immunohistochemistry, Mouse Assay

    Antiapoptotic BCL-2 family proteins block apoptosis and promote tumor formation. ( A ) Generation of stable cell lines. Cell extracts made from stable BMK cells that express both BAX and BAK (W2), or that are deficient for BAX and BAK (D3), were subjected to Western blotting with antibodies specific for BCL-2 ( left top panel) or E1B 19K ( right top panel). Note the similar expression levels of each exogenous protein in three independent clones (depicted numerically) and undetectable levels of each exogenous protein in the vector-only control cell lines (W2.3.1–2,5,6 or D3.zeo-1,2,3). Blots were then reprobed with an antibody to actin to verify nearly equivalent levels of protein in all lanes, shown below the BCL-2 and E1B 19K panels. ( B ) BCL-2 and E1B 19K block apoptosis in response to staurosporine. Stable BMK cell lines expressing BCL-2, E1B 19K, and controls were treated with media alone (open bars) or media containing 0.4 μM staurosporine (filled bars) for 24 h, and the viable cell number was determined by trypan blue exclusion. Results are presented as the percent of viable cells in each condition, which in each case represents the average of three independent plates. ( C ) BCL-2 and E1B 19K antagonize BAX and BAK to promote tumor formation. Three independent stable BMK cell lines (circles, squares, and diamonds) expressing BCL-2 (green symbols), E1B 19K (blue symbols), or controls (red symbols) were injected subcutaneously into nude mice, and tumor formation was monitored over time. Each point represents the average tumor volume for five injected animals. W2 cells, which express both BAX and BAK, are shown in the left panel. D3 cells, which are deficient for both BAX and BAK, are shown in the right panel. Note that BCL-2 or E1B 19K expression caused a profound acceleration of tumor formation in the W2 cells, whereas the kinetics of tumor formation in the D3 cells, which are deficient for both BAX and BAK, were unchanged by BCL-2 or E1B 19K expression.

    Journal: Genes & Development

    Article Title: Hypoxia and defective apoptosis drive genomic instability and tumorigenesis

    doi: 10.1101/gad.1204904

    Figure Lengend Snippet: Antiapoptotic BCL-2 family proteins block apoptosis and promote tumor formation. ( A ) Generation of stable cell lines. Cell extracts made from stable BMK cells that express both BAX and BAK (W2), or that are deficient for BAX and BAK (D3), were subjected to Western blotting with antibodies specific for BCL-2 ( left top panel) or E1B 19K ( right top panel). Note the similar expression levels of each exogenous protein in three independent clones (depicted numerically) and undetectable levels of each exogenous protein in the vector-only control cell lines (W2.3.1–2,5,6 or D3.zeo-1,2,3). Blots were then reprobed with an antibody to actin to verify nearly equivalent levels of protein in all lanes, shown below the BCL-2 and E1B 19K panels. ( B ) BCL-2 and E1B 19K block apoptosis in response to staurosporine. Stable BMK cell lines expressing BCL-2, E1B 19K, and controls were treated with media alone (open bars) or media containing 0.4 μM staurosporine (filled bars) for 24 h, and the viable cell number was determined by trypan blue exclusion. Results are presented as the percent of viable cells in each condition, which in each case represents the average of three independent plates. ( C ) BCL-2 and E1B 19K antagonize BAX and BAK to promote tumor formation. Three independent stable BMK cell lines (circles, squares, and diamonds) expressing BCL-2 (green symbols), E1B 19K (blue symbols), or controls (red symbols) were injected subcutaneously into nude mice, and tumor formation was monitored over time. Each point represents the average tumor volume for five injected animals. W2 cells, which express both BAX and BAK, are shown in the left panel. D3 cells, which are deficient for both BAX and BAK, are shown in the right panel. Note that BCL-2 or E1B 19K expression caused a profound acceleration of tumor formation in the W2 cells, whereas the kinetics of tumor formation in the D3 cells, which are deficient for both BAX and BAK, were unchanged by BCL-2 or E1B 19K expression.

    Article Snippet: Primary antibodies used were hamster anti-human BCL-2 (BD-Pharmingen); rabbit polyclonal anti-E1B 19K , mouse anti-actin (Oncogene Research Products), rabbit anti-mouse HIF-1α (Cayman), rabbit anti-BIM (Axxora), or rabbit polyclonal antisera raised in our lab against a GST-human PUMA fusion protein encoding a 102-amino acid region common to PUMA-α and PUMA-β.

    Techniques: Blocking Assay, Stable Transfection, Western Blot, Expressing, Clone Assay, Plasmid Preparation, Injection, Mouse Assay

    Expression of glutamate receptors. (A) Representative western-blot analysis of GluR1, NR2A, and NR2B subunits in the ACC homogenates. Levels of GluR1 (B) , NR2A (C), and NR2B (D) in saline control, vehicle-, T3-, and T4-treated HT mice. n = 5 in each group. * p

    Journal: Molecular Pain

    Article Title: Decreased pain threshold and enhanced synaptic transmission in the anterior cingulate cortex of experimental hypothyroidism mice

    doi: 10.1186/1744-8069-10-38

    Figure Lengend Snippet: Expression of glutamate receptors. (A) Representative western-blot analysis of GluR1, NR2A, and NR2B subunits in the ACC homogenates. Levels of GluR1 (B) , NR2A (C), and NR2B (D) in saline control, vehicle-, T3-, and T4-treated HT mice. n = 5 in each group. * p

    Article Snippet: Rabbit anti-NR2A and anti-NR2B were purchased from Millipore (Billerica, MA).

    Techniques: Expressing, Western Blot, Mouse Assay

    Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit IgG and a secondary antibody labeled with Cy3. The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.

    Journal: PLoS ONE

    Article Title: Fibroblast Growth Factor Receptors (FGFRs) in Human Sperm: Expression, Functionality and Involvement in Motility Regulation

    doi: 10.1371/journal.pone.0127297

    Figure Lengend Snippet: Localization of FGFRs in human sperm. Sperm cells were stained with anti FGFR1, FGFR2, FGFR3 and FGFR4 or rabbit IgG and a secondary antibody labeled with Cy3. The corresponding fields stained with FITC-PSA to assess acrosomal status are shown. Bar: 10 μm. On the right, a representative image of individual sperm is depicted; ( A ) sperm stained with anti FGFR antibody and Cy3-conjugated secondary antibody, ( B ) FITC-PSA, ( C ) merge.

    Article Snippet: Other antibodies used were: anti pFGFR Tyr653/654 (#3476, Cell Signaling Technology, Inc., Beverley, MA, USA), anti pERK (sc-7383, Santa Cruz, and #4370, Cell Signaling), anti ERK (sc-94, Santa Cruz), anti pAkt Ser473 (sc-7985, Santa Cruz, and #4060, Cell Signaling), anti Akt (#4691, Cell Signaling), rabbit immunoglobulin G (IgG) (Sigma), horse-radish peroxidase (HRP)-conjugated anti-rabbit IgG (Sigma), Cy3-conjugated anti-rabbit IgG and FITC-conjugated anti-mouse IgG (Chemicon-Millipore, Billerica, MA, USA) and anti-rabbit IgG (Sigma).

    Techniques: Staining, Labeling