Journal: Tropical Medicine and Infectious Disease

Article Title: Echinococcus multilocularis Calreticulin Interferes with C1q-Mediated Complement Activation

doi: 10.3390/tropicalmed8010047

Figure Lengend Snippet: r Em CRT inhibited human C1q to bind to IgM. Total 10 μg/mL of C1q was pre-incubated with 0.5 to 3 times excess ( w : w ) of r Em CRT or BSA and then transferred to the plates coated with IgM. After being washed, anti-C1q polyclonal antibody was used to detect C1q binding to IgM in presence of r Em CRT. Data are expressed as mean ± SDs from three independent experiments and statistical analysis was performed using one-way ANOVA. **** p < 0.0001 compared to BSA control.

Article Snippet: After being washed for three times with PBST, each well was added with 100 µL goat anti-human C4 mAb (1:1000 Abcam, Cambridge, UK) or rabbit anti-human C3 polyclonal antibodies (1:100, BOSTER Biological Technology Co., Ltd..., Chengmai, China) to determine C4 and C3 intermediate product of classical complement activation.

Techniques: Incubation, Binding Assay

Journal: iScience

Article Title: Mulberroside A alleviates osteoarthritis via restoring impaired autophagy and suppressing MAPK/NF-κB/PI3K-AKT-mTOR signaling pathways

doi: 10.1016/j.isci.2023.105936

Figure Lengend Snippet:

Article Snippet: FITC Conjugated AffiniPure Goat Anti-rabbit IgG , Boster, Wuhan, Hubei, China , BA1105.

Techniques: Recombinant, Isolation, Lysis, CCK-8 Assay, Staining, Software

Journal: iScience

Article Title: Mulberroside A alleviates osteoarthritis via restoring impaired autophagy and suppressing MAPK/NF-κB/PI3K-AKT-mTOR signaling pathways

doi: 10.1016/j.isci.2023.105936

Figure Lengend Snippet:

Article Snippet: FITC Conjugated AffiniPure Goat Anti-rabbit IgG , Boster, Wuhan, Hubei, China , BA1105.

Techniques: Recombinant, Isolation, Lysis, CCK-8 Assay, Staining, Software