anti rabbit igg  (Nordic-Mubio)

 
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  • 91
    Name:
    Rabbit IgG
    Description:
    Chromatographically purified Rabbit IgG Rabbit
    Catalog Number:
    RIgG
    Price:
    [322.26]
    Size:
    100 mg
    Category:
    Antibody
    Antibody Type:
    Isotype control
    Reactivity:
    Rabbit
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    Structured Review

    Nordic-Mubio anti rabbit igg
    Chromatographically purified Rabbit IgG Rabbit
    https://www.bioz.com/result/anti rabbit igg/product/Nordic-Mubio
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg - by Bioz Stars, 2020-08
    91/100 stars

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    Related Articles

    IA:

    Article Title: Impact of viral-mediated IGF-I gene transfer on skeletal muscle following cast immobilization
    Article Snippet: .. Sections were incubated with rabbit anti-laminin (Neomarker; Labvision, Fremont, CA) and mouse anti-embryonic myosin heavy chain antibodies (4°C overnight; DSHB, Iowa City, IA), followed by incubation with rhodamine-conjugated anti-rabbit IgG (Nordic Immunological Laboratories) and FITC-conjugated anti-mouse IgG (Nordic Immunological Laboratories). .. A mouse blocking kit (BMK-2202, Mouse on Mouse; Vector Laboratories) was used for staining of Pax7.

    Incubation:

    Article Title: Impact of viral-mediated IGF-I gene transfer on skeletal muscle following cast immobilization
    Article Snippet: .. Sections were incubated with rabbit anti-laminin (Neomarker; Labvision, Fremont, CA) and mouse anti-embryonic myosin heavy chain antibodies (4°C overnight; DSHB, Iowa City, IA), followed by incubation with rhodamine-conjugated anti-rabbit IgG (Nordic Immunological Laboratories) and FITC-conjugated anti-mouse IgG (Nordic Immunological Laboratories). .. A mouse blocking kit (BMK-2202, Mouse on Mouse; Vector Laboratories) was used for staining of Pax7.

    Article Title: Characterization of Syntenin, a Syndecan-binding PDZ Protein, as a Component of Cell Adhesion Sites and Microfilaments
    Article Snippet: .. Where required, the cells were permeabilized with 0.1% Triton X-100 in PBS, incubated with first antibody and/or Texas Red-X–phalloidin, for 1 h at RT, followed by goat anti-mouse or anti-rabbit IgG conjugated to fluorescein isothiocyanate (Nordic Immunology, Tilburg, The Netherlands) or Texas-Red (Molecular Probes). .. For competition experiments, the GST fusion proteins were prepared as described in and preincubated with the first antibody as described in Figure , K–X.

    Article Title: Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies
    Article Snippet: .. After washing, membranes were incubated with anti-Rabbit-IgG-PO (1 : 30,000) (Nordic Immunology) and finally developed. .. Native serum samples without inhibition and those inhibited with bovine serum albumin (BSA) were used as controls.

    Immunofluorescence:

    Article Title: Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires
    Article Snippet: .. Direct immunofluorescence (IF) was carried out using Rabbit IgG against a pool of the studied serovars of Leptospira (made in-house) and goat anti-rabbit IgG-fluorescein isothiocyanate conjugate (Nordic Immunological Laboratories, Netherlands) following the procedure of . ..

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  • 85
    Nordic-Mubio igg2b
    Induction of anti-nucleolar autoantibodies (ANolA) of <t>IgG1</t> and <t>IgG2a</t> isotypes in outbred Institute of Cancer Research (ICR), Naval Medical Research Institute (NMRI) and Black Swiss mice treated with silver nitrate (AgNO 3 ). Groups of female ICR (solid and open circles), NMRI (solid and open squares) and Black Swiss (solid and open hexagons) outbred mice were injected repeatedly subcutaneously (s.c.) with AgNO 3 (solid symbols) or NaCl (open symbols) for 4 weeks. At the end of each experiment the mice were bled and killed. The sera were tested for the presence of immunoglobulin (Ig)G1 (a) and IgG2a (b) ANolA by using an indirect immunofluorescence (IIF) method. Each symbol represents a single animal. The mean values ± standard error are presented as thin vertical lines. Values in parentheses represent the proportions of silver-treated animals, which produce ANolA.
    Igg2b, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg2b/product/Nordic-Mubio
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    igg2b - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    85
    Nordic-Mubio fitc conjugated goat anti mouse igg antibodies
    Cell-surface expression of PFF0620C, PFD1130W or PF14_0325 on stably transfected HEK cells . Fluorescence staining of living or methanol fixed untransfected HEK cells, PFF0620C-HEK cells, PFD1130w-HEK cells and PF14_0325 HEK cells after staining with anti-His tag or anti-FLAG tag antibodies and <t>FITC-labelled</t> anti-mouse <t>IgG</t> antibodies. Nuclei were stained with DAPI. With the anti-FLAG tag antibody both living and methanol-fixed transfectants were stained, whereas the anti-His tag antibody only stained methanol-fixed transfectants, indicating intracellular localisation of the His tag and extracellular localisation of the FLAG tag together with the P. falciparum derived protein domain.
    Fitc Conjugated Goat Anti Mouse Igg Antibodies, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated goat anti mouse igg antibodies/product/Nordic-Mubio
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated goat anti mouse igg antibodies - by Bioz Stars, 2020-08
    85/100 stars
      Buy from Supplier

    Image Search Results


    Induction of anti-nucleolar autoantibodies (ANolA) of IgG1 and IgG2a isotypes in outbred Institute of Cancer Research (ICR), Naval Medical Research Institute (NMRI) and Black Swiss mice treated with silver nitrate (AgNO 3 ). Groups of female ICR (solid and open circles), NMRI (solid and open squares) and Black Swiss (solid and open hexagons) outbred mice were injected repeatedly subcutaneously (s.c.) with AgNO 3 (solid symbols) or NaCl (open symbols) for 4 weeks. At the end of each experiment the mice were bled and killed. The sera were tested for the presence of immunoglobulin (Ig)G1 (a) and IgG2a (b) ANolA by using an indirect immunofluorescence (IIF) method. Each symbol represents a single animal. The mean values ± standard error are presented as thin vertical lines. Values in parentheses represent the proportions of silver-treated animals, which produce ANolA.

    Journal: Clinical and Experimental Immunology

    Article Title: Mercury and silver induce B cell activation and anti-nucleolar autoantibody production in outbred mouse stocks: are environmental factors more important than the susceptibility genes in connection with autoimmunity?

    doi: 10.1111/j.1365-2249.2008.03801.x

    Figure Lengend Snippet: Induction of anti-nucleolar autoantibodies (ANolA) of IgG1 and IgG2a isotypes in outbred Institute of Cancer Research (ICR), Naval Medical Research Institute (NMRI) and Black Swiss mice treated with silver nitrate (AgNO 3 ). Groups of female ICR (solid and open circles), NMRI (solid and open squares) and Black Swiss (solid and open hexagons) outbred mice were injected repeatedly subcutaneously (s.c.) with AgNO 3 (solid symbols) or NaCl (open symbols) for 4 weeks. At the end of each experiment the mice were bled and killed. The sera were tested for the presence of immunoglobulin (Ig)G1 (a) and IgG2a (b) ANolA by using an indirect immunofluorescence (IIF) method. Each symbol represents a single animal. The mean values ± standard error are presented as thin vertical lines. Values in parentheses represent the proportions of silver-treated animals, which produce ANolA.

    Article Snippet: The numbers of splenic cells secreting antibodies belonging to different Ig classes and subclasses were quantified utilizing the protein A plaque assay described by Gronowicz et al. [ ], employing rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC, USA) and IgG2b (Nordic Immunological Laboratories, Tillburg, the Netherlands) as the developing reagents.

    Techniques: Mouse Assay, Injection, Immunofluorescence

    Induction of anti-nucleolar autoantibodies (ANolA) of IgG1 and IgG2a isotypes in outbred Institute of Cancer Research (ICR), Naval Medical Research Institute (NMRI) and Black Swiss mice treated with mercuric chloride (HgCl 2 ). Groups of female ICR (solid and open circles), NMRI (solid and open squares) and Black Swiss (solid and open hexagons) outbred mice were injected repeatedly subcutaneously (s.c.) with HgCl 2 (solid symbols) or NaCl (open symbols) for 4 weeks. At the end of each experiment the mice were bled and killed. The sera were tested for the presence of IgG1 (a) and IgG2a (b) ANolA by using an indirect immunofluorescence (IIF) method. Each symbol represents a single animal. The mean values ± standard error are presented as thin vertical lines. Values in parentheses represent the proportions of mercury-treated animals, which produce ANolA.

    Journal: Clinical and Experimental Immunology

    Article Title: Mercury and silver induce B cell activation and anti-nucleolar autoantibody production in outbred mouse stocks: are environmental factors more important than the susceptibility genes in connection with autoimmunity?

    doi: 10.1111/j.1365-2249.2008.03801.x

    Figure Lengend Snippet: Induction of anti-nucleolar autoantibodies (ANolA) of IgG1 and IgG2a isotypes in outbred Institute of Cancer Research (ICR), Naval Medical Research Institute (NMRI) and Black Swiss mice treated with mercuric chloride (HgCl 2 ). Groups of female ICR (solid and open circles), NMRI (solid and open squares) and Black Swiss (solid and open hexagons) outbred mice were injected repeatedly subcutaneously (s.c.) with HgCl 2 (solid symbols) or NaCl (open symbols) for 4 weeks. At the end of each experiment the mice were bled and killed. The sera were tested for the presence of IgG1 (a) and IgG2a (b) ANolA by using an indirect immunofluorescence (IIF) method. Each symbol represents a single animal. The mean values ± standard error are presented as thin vertical lines. Values in parentheses represent the proportions of mercury-treated animals, which produce ANolA.

    Article Snippet: The numbers of splenic cells secreting antibodies belonging to different Ig classes and subclasses were quantified utilizing the protein A plaque assay described by Gronowicz et al. [ ], employing rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC, USA) and IgG2b (Nordic Immunological Laboratories, Tillburg, the Netherlands) as the developing reagents.

    Techniques: Mouse Assay, Injection, Immunofluorescence

    Mercury induces antibody production of various isotypes in young NOD mice. Groups of young female NOD mice (3–12 mice/group) were repeatedly injected s.c. with mercuric chloride (•). Control mice were either injected with sterile saline or left uninjected (○). At different times after injections, the mice were bled and killed. The spleens were tested for IgM, IgG1, IgG2b and IgG3 antibody secreting cells by using a protein A plaque assay and the sera were tested for IgE by using an ELISA method. Data are shown as the means + 1 s.e. Significant differences between the parameters in mercury- and saline-injected mice were calculated by Wilcoxon–Mann–Whitney test. Tukey's test was used to correct the P -values. * P

    Journal: Clinical and Experimental Immunology

    Article Title: Analysis of mercury-induced immune activation in nonobese diabetic (NOD) mice

    doi: 10.1046/j.1365-2249.2001.01580.x

    Figure Lengend Snippet: Mercury induces antibody production of various isotypes in young NOD mice. Groups of young female NOD mice (3–12 mice/group) were repeatedly injected s.c. with mercuric chloride (•). Control mice were either injected with sterile saline or left uninjected (○). At different times after injections, the mice were bled and killed. The spleens were tested for IgM, IgG1, IgG2b and IgG3 antibody secreting cells by using a protein A plaque assay and the sera were tested for IgE by using an ELISA method. Data are shown as the means + 1 s.e. Significant differences between the parameters in mercury- and saline-injected mice were calculated by Wilcoxon–Mann–Whitney test. Tukey's test was used to correct the P -values. * P

    Article Snippet: Rabbit antimouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC, USA) and IgG2b (Nordic Immunological Laboratories, Tillburg, The Netherlands) were used as developing reagents.

    Techniques: Mouse Assay, Injection, Plaque Assay, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Mercury-induced renal IgG deposits of various antibody isotypes in both C57BL/6 wild-type and IL-4-deficient mice

    Journal: Immunology

    Article Title: Mechanism of mercury-induced autoimmunity: both T helper 1- and T helper 2-type responses are involved

    doi: 10.1046/j.1365-2567.1999.00671.x

    Figure Lengend Snippet: Mercury-induced renal IgG deposits of various antibody isotypes in both C57BL/6 wild-type and IL-4-deficient mice

    Article Snippet: Rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC) and IgG2b (Nordic Immunological Laboratories, Tilburg, The Netherlands) were used as developing reagents.

    Techniques: Mouse Assay

    Downregulation of IgG2a and IgG3 but not IgG2b antibody production by anti-IFN-γ antibody treatment in mercury-treated C57BL/6 IL-4-deficient mice. C57BL/6 IL-4-deficient mice (three to four mice per group) were treated with saline or mercury and anti-IFN-γ or control antibodies as described in the Materials and Methods. After 4 weeks, the IgG2b and IgG3 antibody-producing cells in the spleens were measured by PFC (a) and serum IgG2a levels were measured by ELISA (b). Data are shown as the means of PFC/spleen±1 SD or absorbance values±1 SD. * P

    Journal: Immunology

    Article Title: Mechanism of mercury-induced autoimmunity: both T helper 1- and T helper 2-type responses are involved

    doi: 10.1046/j.1365-2567.1999.00671.x

    Figure Lengend Snippet: Downregulation of IgG2a and IgG3 but not IgG2b antibody production by anti-IFN-γ antibody treatment in mercury-treated C57BL/6 IL-4-deficient mice. C57BL/6 IL-4-deficient mice (three to four mice per group) were treated with saline or mercury and anti-IFN-γ or control antibodies as described in the Materials and Methods. After 4 weeks, the IgG2b and IgG3 antibody-producing cells in the spleens were measured by PFC (a) and serum IgG2a levels were measured by ELISA (b). Data are shown as the means of PFC/spleen±1 SD or absorbance values±1 SD. * P

    Article Snippet: Rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC) and IgG2b (Nordic Immunological Laboratories, Tilburg, The Netherlands) were used as developing reagents.

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Mercury induced various antibody production in the spleens of C57BL/6 wild-type and IL-4-deficient mice. Groups of mice were repeatedly injected s.c. with either HgCl 2 or saline. At different times after injections, spleens of three to eight mice were tested for IgM, IgG1, IgG2b and IgG3 antibody-secreting cells. Data are shown as the means of PFC/spleen±1 SD. Significant differences between mercury- and saline-injected mice were calculated by Wilcoxon–Mann–Whitney test, except the data of IL-4-deficient mice on weeks 6 and 8, to which a Student’s t -test was applied because of the small number of mice (two per group) used in saline control groups, * P

    Journal: Immunology

    Article Title: Mechanism of mercury-induced autoimmunity: both T helper 1- and T helper 2-type responses are involved

    doi: 10.1046/j.1365-2567.1999.00671.x

    Figure Lengend Snippet: Mercury induced various antibody production in the spleens of C57BL/6 wild-type and IL-4-deficient mice. Groups of mice were repeatedly injected s.c. with either HgCl 2 or saline. At different times after injections, spleens of three to eight mice were tested for IgM, IgG1, IgG2b and IgG3 antibody-secreting cells. Data are shown as the means of PFC/spleen±1 SD. Significant differences between mercury- and saline-injected mice were calculated by Wilcoxon–Mann–Whitney test, except the data of IL-4-deficient mice on weeks 6 and 8, to which a Student’s t -test was applied because of the small number of mice (two per group) used in saline control groups, * P

    Article Snippet: Rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC) and IgG2b (Nordic Immunological Laboratories, Tilburg, The Netherlands) were used as developing reagents.

    Techniques: Mouse Assay, Injection, MANN-WHITNEY

    Serum antibody levels of different isotypes in mercury-treated C57BL/6 IL-4-deficient mice. Groups of mice were repeatedly injected s.c. with either HgCl 2 (solid symbols) or saline (open symbols). At week 4 (w4) and 6 (w6), pooled sera collected from the blood of three to four experimental mice were measured for serum levels of IgM (a), IgG1 (b), IgG2a (c) IgG2b (d) and IgG3(e) antibodies. In the case of IgE antibody, the serum levels of this antibody (f) were measured at different times: solid circle (day 7, mercury-treated), solid triangle (day 14, mercury-treated), solid square (week 4, mercury-treated) and open square (week 4, saline-treated). Since similar levels of IgE antibodies were found in the saline-treated mice at different times, we showed only the serum IgE levels for week 4 in these mice.

    Journal: Immunology

    Article Title: Mechanism of mercury-induced autoimmunity: both T helper 1- and T helper 2-type responses are involved

    doi: 10.1046/j.1365-2567.1999.00671.x

    Figure Lengend Snippet: Serum antibody levels of different isotypes in mercury-treated C57BL/6 IL-4-deficient mice. Groups of mice were repeatedly injected s.c. with either HgCl 2 (solid symbols) or saline (open symbols). At week 4 (w4) and 6 (w6), pooled sera collected from the blood of three to four experimental mice were measured for serum levels of IgM (a), IgG1 (b), IgG2a (c) IgG2b (d) and IgG3(e) antibodies. In the case of IgE antibody, the serum levels of this antibody (f) were measured at different times: solid circle (day 7, mercury-treated), solid triangle (day 14, mercury-treated), solid square (week 4, mercury-treated) and open square (week 4, saline-treated). Since similar levels of IgE antibodies were found in the saline-treated mice at different times, we showed only the serum IgE levels for week 4 in these mice.

    Article Snippet: Rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC) and IgG2b (Nordic Immunological Laboratories, Tilburg, The Netherlands) were used as developing reagents.

    Techniques: Mouse Assay, Injection

    Serum antibody levels of different isotypes in mercury-treated C57BL/6 wild-type mice. Groups of mice were repeatedly injected s.c. with either HgCl 2 (solid symbols) or saline (open symbols). At week 4 (w4) and 6 (w6), pooled sera collected from the blood of three to four experimental mice were measured for serum levels of IgM (a), IgG1 (b), IgG2a (c) IgG2b (d) and IgG3 (e) antibodies. In the case of IgE antibody, the serum levels of this antibody (f) were measured at different times: solid circle (day 7, mercury treated), solid triangle (day 14, mercury treated), solid square (week 4, mercury treated) and open square (week 4, saline treated). As similar levels of IgE antibodies were found in the saline-treated mice at different times, we showed only the serum IgE levels for week 4 in these mice.

    Journal: Immunology

    Article Title: Mechanism of mercury-induced autoimmunity: both T helper 1- and T helper 2-type responses are involved

    doi: 10.1046/j.1365-2567.1999.00671.x

    Figure Lengend Snippet: Serum antibody levels of different isotypes in mercury-treated C57BL/6 wild-type mice. Groups of mice were repeatedly injected s.c. with either HgCl 2 (solid symbols) or saline (open symbols). At week 4 (w4) and 6 (w6), pooled sera collected from the blood of three to four experimental mice were measured for serum levels of IgM (a), IgG1 (b), IgG2a (c) IgG2b (d) and IgG3 (e) antibodies. In the case of IgE antibody, the serum levels of this antibody (f) were measured at different times: solid circle (day 7, mercury treated), solid triangle (day 14, mercury treated), solid square (week 4, mercury treated) and open square (week 4, saline treated). As similar levels of IgE antibodies were found in the saline-treated mice at different times, we showed only the serum IgE levels for week 4 in these mice.

    Article Snippet: Rabbit anti-mouse IgM, IgG1, IgG3 (Organon Teknika, Durham, NC) and IgG2b (Nordic Immunological Laboratories, Tilburg, The Netherlands) were used as developing reagents.

    Techniques: Mouse Assay, Injection

    Cell-surface expression of PFF0620C, PFD1130W or PF14_0325 on stably transfected HEK cells . Fluorescence staining of living or methanol fixed untransfected HEK cells, PFF0620C-HEK cells, PFD1130w-HEK cells and PF14_0325 HEK cells after staining with anti-His tag or anti-FLAG tag antibodies and FITC-labelled anti-mouse IgG antibodies. Nuclei were stained with DAPI. With the anti-FLAG tag antibody both living and methanol-fixed transfectants were stained, whereas the anti-His tag antibody only stained methanol-fixed transfectants, indicating intracellular localisation of the His tag and extracellular localisation of the FLAG tag together with the P. falciparum derived protein domain.

    Journal: BMC Biotechnology

    Article Title: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

    doi: 10.1186/1472-6750-10-87

    Figure Lengend Snippet: Cell-surface expression of PFF0620C, PFD1130W or PF14_0325 on stably transfected HEK cells . Fluorescence staining of living or methanol fixed untransfected HEK cells, PFF0620C-HEK cells, PFD1130w-HEK cells and PF14_0325 HEK cells after staining with anti-His tag or anti-FLAG tag antibodies and FITC-labelled anti-mouse IgG antibodies. Nuclei were stained with DAPI. With the anti-FLAG tag antibody both living and methanol-fixed transfectants were stained, whereas the anti-His tag antibody only stained methanol-fixed transfectants, indicating intracellular localisation of the His tag and extracellular localisation of the FLAG tag together with the P. falciparum derived protein domain.

    Article Snippet: After washing two times with serum-free culture medium 500 μl of 100 μg/ml FITC-conjugated goat anti-mouse IgG antibodies (RAM/IgG(H+L)/FITC, Nordic Immunological Laboratories) diluted in serum-free culture medium were added to the wells and incubated for 30 min on ice.

    Techniques: Expressing, Stable Transfection, Transfection, Fluorescence, Staining, FLAG-tag, Derivative Assay

    Immunofluorescence microscopic screening of hybridoma cell culture supernatants for antibodies binding to cell-surface expressed PFF0620C . PFF0620C-HEK cells (right column) and untransfected HEK cells (left column) were fixed with methanol and stained with hybridoma supernatants of individual cell culture wells taken 15 days after the fusion. A FITC-labelled anti-mouse IgG antibody served as secondary antibody. Representative fluorescence micrographs from the PFF0620C-fusion are shown, demonstrating typical results for hybidoma lines producing PFF0620C-specific (line 1, 2 3), non-binding (line 4), or HEK cell antigen specific (line 5 6) antibodies. For some wells mixed specificity was seen, indicating presence of two ore more cell clones with one producing Abs specific for the transgene and the other producing Abs specific for a HEK-cell protein (line 7).

    Journal: BMC Biotechnology

    Article Title: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

    doi: 10.1186/1472-6750-10-87

    Figure Lengend Snippet: Immunofluorescence microscopic screening of hybridoma cell culture supernatants for antibodies binding to cell-surface expressed PFF0620C . PFF0620C-HEK cells (right column) and untransfected HEK cells (left column) were fixed with methanol and stained with hybridoma supernatants of individual cell culture wells taken 15 days after the fusion. A FITC-labelled anti-mouse IgG antibody served as secondary antibody. Representative fluorescence micrographs from the PFF0620C-fusion are shown, demonstrating typical results for hybidoma lines producing PFF0620C-specific (line 1, 2 3), non-binding (line 4), or HEK cell antigen specific (line 5 6) antibodies. For some wells mixed specificity was seen, indicating presence of two ore more cell clones with one producing Abs specific for the transgene and the other producing Abs specific for a HEK-cell protein (line 7).

    Article Snippet: After washing two times with serum-free culture medium 500 μl of 100 μg/ml FITC-conjugated goat anti-mouse IgG antibodies (RAM/IgG(H+L)/FITC, Nordic Immunological Laboratories) diluted in serum-free culture medium were added to the wells and incubated for 30 min on ice.

    Techniques: Immunofluorescence, Cell Culture, Binding Assay, Staining, Fluorescence, Clone Assay