anti rabbit igg  (New England Biolabs)


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    Name:
    Goat Anti Rabbit IgG Magnetic Beads
    Description:
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    Catalog Number:
    s1432s
    Price:
    188
    Size:
    20 mg
    Category:
    Magnetic Separation Equipment
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    Structured Review

    New England Biolabs anti rabbit igg
    Goat Anti Rabbit IgG Magnetic Beads
    Goat Anti Rabbit IgG Magnetic Beads 20 mg
    https://www.bioz.com/result/anti rabbit igg/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg - by Bioz Stars, 2020-09
    95/100 stars

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    Related Articles

    RNA Sequencing Assay:

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Magnetic Beads:

    Article Title: Disturbed flow increases UBE2C via loss of miR-483-3p, inducing aortic valve calcification by the HIF1α pathway in endothelial cells
    Article Snippet: .. For pVHL or ubiquitin immunoprecitations, 25 μl of goat-Anti-Rabbit IgG (New England Biolabs, S1432S) or goat-Anti-Mouse IgG (New England Biolabs, S1431S) magnetic beads were pre-incubated (2 hour at RT) with 5 μg of anti-VHL antibody (Thermo Fisher Scientific PA5–27322) or an anti-total-ubiquitin antibody (Abcam ab128424). .. Cells were lysed using an IP lysis buffer (Thermo Fisher Scientific 87787) and added to the magnetic beads overnight at 4C.

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Blocking Assay:

    Article Title: In vivo effects of rosiglitazone in a human neuroblastoma xenograft
    Article Snippet: .. After blocking (5% skimmed milk for 2 h), nitrocellulose membranes were washed and then immunostained with a rabbit anti-human PPARγ antibody (1 : 1000) (Santa Cruz Biotechnology, Santa Cruz, CA, USA) or with an anti-phospho-PPARγ (Ser82) clone AW 504 (1 : 1000) (Upstate, Lake Placid, NY, USA), followed by a secondary anti-rabbit IgG antibody (1 : 2000) (New England Biolabs, Beverly, MA, USA). .. The antibody-reacted proteins were revealed by LumiGLO chemiluminescent reagent and peroxide (New England Biolabs).

    Immunoprecipitation:

    Article Title: Fetal lung C4BPA induces p100 processing in human placenta
    Article Snippet: .. Immunoprecipitation of exosomes We coupled goat-anti-rabbit immunomagnetic beads (New England Biolabs, USA) coated with anti-AFP or C4BPA antibody by mixing them and rotating at 4 °C for 1 hr. .. The beads were washed three times with immunoprecipitation buffer (IP buffer; 10 mM Tris [pH 7.2], 10 mM MgCl2 , 200 mM KCl, 0.1% Triton X-100).

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    Incubation:

    Article Title: Beneficial Effects of Resistance Exercise on Glycemic Control Are Not Further Improved by Protein Ingestion
    Article Snippet: .. The membrane was then incubated for 1 h at room temperature within wash buffer containing the appropriate secondary antibody, either horseradish (HRP)-linked anti-mouse IgG (New England Biolabs, 7072; 1∶1,000) or anti-rabbit IgG (New England Biolabs, 7074; 1∶1,000). .. The membrane was then cleared in wash buffer three times for 5 min. Antibody binding was detected using enhanced chemiluminescence (Millipore, Billerica, MA).

    Article Title: Eukaryotic translation elongation factor-1 alpha is associated with a specific subset of mRNAs in Trypanosoma cruzi
    Article Snippet: .. EF-1α immunoprecipitation RNA-seq To identify mRNAs associated with EF-1α protein or complexes, rabbit anti-EF-1α antibody (15 μl) was incubated with 50 μl of goat anti-rabbit magnetic beads (New England Biolabs) and 40 U/ml RNAse OUT (Invitrogen) for 16 h at 4 °C with shaking. .. Cytoplasmic extract corresponding to 1 × 109 cells was then incubated with the beads.

    other:

    Article Title: Decisive role of P42/44 mitogen-activated protein kinase in Δ9-tetrahydrocannabinol-induced migration of human mesenchymal stem cells
    Article Snippet: The following antibody dilutions were used: 1:5000 (β-actin) and 1:1000 (p42/44, phospho-p42/44, anti-mouse IgG, anti-rabbit IgG).

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  • 92
    New England Biolabs horseradish peroxidase hrp conjugated anti rabbit igg
    Expression levels of the endogenously tagged TFB3 in UV-treated and untreated S. acidocaldarius ( tfb3 c SF) cells. ( A ) SDS-PAGE (12.5% (w/v) polyacrylamide; Coomassie staining) of crude extracts of tfb3 C SF before the treatment (control) and 45, 90 and 180 min after the treatment with 100 J/m 2 UV irradiation (+UV) or without UV irradiation (−UV). A total of 30 μg of protein extract was loaded per lane. M, PageRuler unstained protein ladder (Thermo Fisher Scientific). ( B ) Autoradiograph showing the expression of TFB3 (23 kDa) and TFB1 (35 kDa) before the treatment (control) and 45, 90 and 180 min after the treatment with (+UV) or without UV irradiation (−UV). To monitor both proteins, which show significant differences in size and expression level, the membrane was cut after the transfer and the parts were handled separately. For TFB3, an anti-FLAG-tag antibody was used as primary antibody with a dilution of 1:1000. As an internal loading control, TFB1 was detected using a polyclonal antibody raised in rabbit (dilution: 1:10 000). Both TFB1 and TFB3 were visualized using a <t>HRP-coupled</t> <t>anti-rabbit-IgG</t> as secondary antibody. The exposition time was 2 min for TFB3 and 5 s for TFB1. M, PageRuler prestained protein ladder (Thermo Fisher Scientific).
    Horseradish Peroxidase Hrp Conjugated Anti Rabbit Igg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/horseradish peroxidase hrp conjugated anti rabbit igg/product/New England Biolabs
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    horseradish peroxidase hrp conjugated anti rabbit igg - by Bioz Stars, 2020-09
    92/100 stars
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    91
    New England Biolabs anti igg
    SRF binds to the longevity allele A but not to the major allele G of rs4946935. Nuclear extracts from Panc1 a and Jurkat b cells were submitted to EMSA with the indicated oligonucleotides. Supershift experiments were performed with antibodies as listed. The position of the supershifted complex is indicated by an arrow. One biological replicate of four is shown. Gt goat, <t>IgG</t> immunoglobulin G, NC nonspecific control (NC I: <t>anti-STAT5A,</t> rabbit; NC II: anti-PDX1, rabbit; NC III, anti-PDX1, goat); PDX1 pancreatic and duodenal homeobox 1, rb rabbit, SRF serum response factor
    Anti Igg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti igg/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti igg - by Bioz Stars, 2020-09
    91/100 stars
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    88
    New England Biolabs anti mbp hrp
    PP1 and Cwc21 bind Snu114 domain ‘IVa’ . Pull down of purified recombinant <t>MBP</t> tagged (a) hSnu114/ snu114-787/9A-∆N (b) ySnu114/ snu114-814/6/8A -∆N with GST tagged rPP1 on glutathione beads. (c) Pull down of MBP tagged ySnu114/ snu114-814/6/8A-∆N with GST tagged Cwc21 on glutathione beads. The bead-bound proteins were recovered and analyzed by western blot, probing with anti MBP <t>HRP</t> antibodies. GST protein was used as a control for specificity. (d) Pull down of GST tagged rPP1 and/or Cwc21 on Amylose beads. Equal amounts (15 pmol) of rPP1 and hSnu114 or ySnu114 were incubated for 2 h at 4ºC. Increasing amounts (15–170 pmol) of GST-Cwc21 were added and further incubated for 2 hours. Proteins were analyzed by western blot, probing with anti-GST HRP antibodies to identify GST tagged PP1 and Cwc21.
    Anti Mbp Hrp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mbp hrp/product/New England Biolabs
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    anti mbp hrp - by Bioz Stars, 2020-09
    88/100 stars
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    91
    New England Biolabs rabbit polyclonal igg nox4 antibody
    <t>Nox4</t> expression in human tissues and cultured cells and intracellular Nox4 localization in human cell lines . (A) Real-time (RT) q-PCR analyses of Nox4 in human brain and liver as well as in SH-SY5Y neuroblastoma and HepG2 hepatoma cells. Values expressed as fold change relative to a housekeeping gene (ribosomal protein P0). Determination of significance as described in the Section “ Materials and Methods .” (B) RT q-PCR analyses of Nox4 in a hepatocellular carcinoma. (C) Immunoblot of total protein extracts from SH-SY5Y and HepG2 cells using a <t>polyclonal</t> antibody against Nox4 and loading controls (GAPDH). (D,E) Fluorescence micrographs of HepG2 and SH-SY5Y cells transiently transfected with enhanced green fluorescence protein (eGFP) in-frame fusion construct Nox4-eGFP. DAPI, Mito or ER denotes nuclear staining with DAPI, mitochondrial staining with MitoTracker Red CMXRos, or staining of endoplasmatic reticulum with ER-Tracker Blue-White DPX, respectively. (F) Immunoblot of Nox4 from a total extract of SH-SY5Y cells and purified microsomes from the same extract with loading control (cofilin) and a control blot with no primary antibody.
    Rabbit Polyclonal Igg Nox4 Antibody, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal igg nox4 antibody/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal igg nox4 antibody - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    Image Search Results


    Expression levels of the endogenously tagged TFB3 in UV-treated and untreated S. acidocaldarius ( tfb3 c SF) cells. ( A ) SDS-PAGE (12.5% (w/v) polyacrylamide; Coomassie staining) of crude extracts of tfb3 C SF before the treatment (control) and 45, 90 and 180 min after the treatment with 100 J/m 2 UV irradiation (+UV) or without UV irradiation (−UV). A total of 30 μg of protein extract was loaded per lane. M, PageRuler unstained protein ladder (Thermo Fisher Scientific). ( B ) Autoradiograph showing the expression of TFB3 (23 kDa) and TFB1 (35 kDa) before the treatment (control) and 45, 90 and 180 min after the treatment with (+UV) or without UV irradiation (−UV). To monitor both proteins, which show significant differences in size and expression level, the membrane was cut after the transfer and the parts were handled separately. For TFB3, an anti-FLAG-tag antibody was used as primary antibody with a dilution of 1:1000. As an internal loading control, TFB1 was detected using a polyclonal antibody raised in rabbit (dilution: 1:10 000). Both TFB1 and TFB3 were visualized using a HRP-coupled anti-rabbit-IgG as secondary antibody. The exposition time was 2 min for TFB3 and 5 s for TFB1. M, PageRuler prestained protein ladder (Thermo Fisher Scientific).

    Journal: Nucleic Acids Research

    Article Title: Effect of UV irradiation on Sulfolobus acidocaldarius and involvement of the general transcription factor TFB3 in the early UV response

    doi: 10.1093/nar/gky527

    Figure Lengend Snippet: Expression levels of the endogenously tagged TFB3 in UV-treated and untreated S. acidocaldarius ( tfb3 c SF) cells. ( A ) SDS-PAGE (12.5% (w/v) polyacrylamide; Coomassie staining) of crude extracts of tfb3 C SF before the treatment (control) and 45, 90 and 180 min after the treatment with 100 J/m 2 UV irradiation (+UV) or without UV irradiation (−UV). A total of 30 μg of protein extract was loaded per lane. M, PageRuler unstained protein ladder (Thermo Fisher Scientific). ( B ) Autoradiograph showing the expression of TFB3 (23 kDa) and TFB1 (35 kDa) before the treatment (control) and 45, 90 and 180 min after the treatment with (+UV) or without UV irradiation (−UV). To monitor both proteins, which show significant differences in size and expression level, the membrane was cut after the transfer and the parts were handled separately. For TFB3, an anti-FLAG-tag antibody was used as primary antibody with a dilution of 1:1000. As an internal loading control, TFB1 was detected using a polyclonal antibody raised in rabbit (dilution: 1:10 000). Both TFB1 and TFB3 were visualized using a HRP-coupled anti-rabbit-IgG as secondary antibody. The exposition time was 2 min for TFB3 and 5 s for TFB1. M, PageRuler prestained protein ladder (Thermo Fisher Scientific).

    Article Snippet: As a secondary antibody, a horseradish peroxidase (HRP)-conjugated anti-rabbit-IgG raised in mouse (NEB) was used with a dilution of 1:10 000.

    Techniques: Expressing, SDS Page, Staining, Irradiation, Autoradiography, FLAG-tag

    SRF binds to the longevity allele A but not to the major allele G of rs4946935. Nuclear extracts from Panc1 a and Jurkat b cells were submitted to EMSA with the indicated oligonucleotides. Supershift experiments were performed with antibodies as listed. The position of the supershifted complex is indicated by an arrow. One biological replicate of four is shown. Gt goat, IgG immunoglobulin G, NC nonspecific control (NC I: anti-STAT5A, rabbit; NC II: anti-PDX1, rabbit; NC III, anti-PDX1, goat); PDX1 pancreatic and duodenal homeobox 1, rb rabbit, SRF serum response factor

    Journal: Nature Communications

    Article Title: Identification and characterization of two functional variants in the human longevity gene FOXO3

    doi: 10.1038/s41467-017-02183-y

    Figure Lengend Snippet: SRF binds to the longevity allele A but not to the major allele G of rs4946935. Nuclear extracts from Panc1 a and Jurkat b cells were submitted to EMSA with the indicated oligonucleotides. Supershift experiments were performed with antibodies as listed. The position of the supershifted complex is indicated by an arrow. One biological replicate of four is shown. Gt goat, IgG immunoglobulin G, NC nonspecific control (NC I: anti-STAT5A, rabbit; NC II: anti-PDX1, rabbit; NC III, anti-PDX1, goat); PDX1 pancreatic and duodenal homeobox 1, rb rabbit, SRF serum response factor

    Article Snippet: For supershift experiments, 4 µg of antibody (anti-SRF (sc335x), anti-PDX1 (sc14664x, goat), anti-STAT5A (sc1081x), anti-CTCF (sc271474x), all Santa Cruz, Heidelberg, Germany; anti-PDX1 (5679x, rabbit), anti-IgG (2729, rabbit), both New England Biolabs GmbH, Frankfurt a.M., Germany); anti-IgG (Merck Chemicals GmbH, Darmstadt, Germany)) were additionally added to the reaction mix 30 min prior to electrophoresis and incubated at 4 °C.

    Techniques:

    PP1 and Cwc21 bind Snu114 domain ‘IVa’ . Pull down of purified recombinant MBP tagged (a) hSnu114/ snu114-787/9A-∆N (b) ySnu114/ snu114-814/6/8A -∆N with GST tagged rPP1 on glutathione beads. (c) Pull down of MBP tagged ySnu114/ snu114-814/6/8A-∆N with GST tagged Cwc21 on glutathione beads. The bead-bound proteins were recovered and analyzed by western blot, probing with anti MBP HRP antibodies. GST protein was used as a control for specificity. (d) Pull down of GST tagged rPP1 and/or Cwc21 on Amylose beads. Equal amounts (15 pmol) of rPP1 and hSnu114 or ySnu114 were incubated for 2 h at 4ºC. Increasing amounts (15–170 pmol) of GST-Cwc21 were added and further incubated for 2 hours. Proteins were analyzed by western blot, probing with anti-GST HRP antibodies to identify GST tagged PP1 and Cwc21.

    Journal: RNA Biology

    Article Title: Mutagenesis of Snu114 domain IV identifies a developmental role in meiotic splicing

    doi: 10.1080/15476286.2018.1561145

    Figure Lengend Snippet: PP1 and Cwc21 bind Snu114 domain ‘IVa’ . Pull down of purified recombinant MBP tagged (a) hSnu114/ snu114-787/9A-∆N (b) ySnu114/ snu114-814/6/8A -∆N with GST tagged rPP1 on glutathione beads. (c) Pull down of MBP tagged ySnu114/ snu114-814/6/8A-∆N with GST tagged Cwc21 on glutathione beads. The bead-bound proteins were recovered and analyzed by western blot, probing with anti MBP HRP antibodies. GST protein was used as a control for specificity. (d) Pull down of GST tagged rPP1 and/or Cwc21 on Amylose beads. Equal amounts (15 pmol) of rPP1 and hSnu114 or ySnu114 were incubated for 2 h at 4ºC. Increasing amounts (15–170 pmol) of GST-Cwc21 were added and further incubated for 2 hours. Proteins were analyzed by western blot, probing with anti-GST HRP antibodies to identify GST tagged PP1 and Cwc21.

    Article Snippet: Anti-GST (B-14) HRP (Mouse monoclonal IgG, Santa-Cruz) was used at 1/10,000 and anti-MBP HRP (Rabbit monoclonal IgG, NEB) was used at 1/8000 dilution.

    Techniques: Purification, Recombinant, Western Blot, Incubation

    Nox4 expression in human tissues and cultured cells and intracellular Nox4 localization in human cell lines . (A) Real-time (RT) q-PCR analyses of Nox4 in human brain and liver as well as in SH-SY5Y neuroblastoma and HepG2 hepatoma cells. Values expressed as fold change relative to a housekeeping gene (ribosomal protein P0). Determination of significance as described in the Section “ Materials and Methods .” (B) RT q-PCR analyses of Nox4 in a hepatocellular carcinoma. (C) Immunoblot of total protein extracts from SH-SY5Y and HepG2 cells using a polyclonal antibody against Nox4 and loading controls (GAPDH). (D,E) Fluorescence micrographs of HepG2 and SH-SY5Y cells transiently transfected with enhanced green fluorescence protein (eGFP) in-frame fusion construct Nox4-eGFP. DAPI, Mito or ER denotes nuclear staining with DAPI, mitochondrial staining with MitoTracker Red CMXRos, or staining of endoplasmatic reticulum with ER-Tracker Blue-White DPX, respectively. (F) Immunoblot of Nox4 from a total extract of SH-SY5Y cells and purified microsomes from the same extract with loading control (cofilin) and a control blot with no primary antibody.

    Journal: Frontiers in Oncology

    Article Title: The Human NADPH Oxidase, Nox4, Regulates Cytoskeletal Organization in Two Cancer Cell Lines, HepG2 and SH-SY5Y

    doi: 10.3389/fonc.2017.00111

    Figure Lengend Snippet: Nox4 expression in human tissues and cultured cells and intracellular Nox4 localization in human cell lines . (A) Real-time (RT) q-PCR analyses of Nox4 in human brain and liver as well as in SH-SY5Y neuroblastoma and HepG2 hepatoma cells. Values expressed as fold change relative to a housekeeping gene (ribosomal protein P0). Determination of significance as described in the Section “ Materials and Methods .” (B) RT q-PCR analyses of Nox4 in a hepatocellular carcinoma. (C) Immunoblot of total protein extracts from SH-SY5Y and HepG2 cells using a polyclonal antibody against Nox4 and loading controls (GAPDH). (D,E) Fluorescence micrographs of HepG2 and SH-SY5Y cells transiently transfected with enhanced green fluorescence protein (eGFP) in-frame fusion construct Nox4-eGFP. DAPI, Mito or ER denotes nuclear staining with DAPI, mitochondrial staining with MitoTracker Red CMXRos, or staining of endoplasmatic reticulum with ER-Tracker Blue-White DPX, respectively. (F) Immunoblot of Nox4 from a total extract of SH-SY5Y cells and purified microsomes from the same extract with loading control (cofilin) and a control blot with no primary antibody.

    Article Snippet: The membrane was washed 30 min with TBS-T (25 mM Tris-HCl pH 7.4, 0.15 M NaCl, 0.5% Tween20, and 0.05% NaN3 ) before blocking unspecific binding sites with MTBS-T [5% (w/v) non-fat dry milk] for 90 min. After another washing step, the membrane was incubated with the primary antibody for 2 h. A rabbit polyclonal IgG Nox4 antibody (New England Biolabs/Cell Signaling; H-300; sc-30141; dilution 1:1,000) was used for detection of Nox4 and a rabbit monoclonal N-WASP IgG antibody (Cell Signaling; 30D10; #4848; dilution 1:2,000) for the detection of N-WASP.

    Techniques: Expressing, Cell Culture, Polymerase Chain Reaction, Fluorescence, Transfection, Construct, Staining, Purification