anti rabbit igg  (Cell Signaling Technology Inc)

 
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    Name:
    Normal Rabbit IgG
    Description:
    Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non specific IgG control in chromatin immunoprecipitation using our SimpleChIP Enzymatic Chromatin IP Kits 9002 and 9003
    Catalog Number:
    2729
    Price:
    None
    Category:
    Primary Antibodies
    Source:
    The purified antibody is not directed against any known antigen. It was isolated from naive rabbit sera and prepared by Protein A chromatography.
    Applications:
    Immunoprecipitation, Chromatin Immunoprecipitation
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    Structured Review

    Cell Signaling Technology Inc anti rabbit igg
    Normal Rabbit IgG is an unconjugated rabbit polyclonal antibody that is routinely used as a non specific IgG control in chromatin immunoprecipitation using our SimpleChIP Enzymatic Chromatin IP Kits 9002 and 9003
    https://www.bioz.com/result/anti rabbit igg/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg - by Bioz Stars, 2021-03
    97/100 stars

    Images

    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Atrophin controls developmental signaling pathways via interactions with Trithorax-like
    Article Snippet: .. ChIP-re-ChIP S2 cells were double crosslinked, sonicated, and ChIP’ed as above, using 5 μL anti-Atro sera (SG2524), and 10 μL normal rabbit IgG (Cell Signaling Technology). ..

    Article Title: T-ALL leukemia stem cell 'stemness' is epigenetically controlled by the master regulator SPI1
    Article Snippet: Approximately 5 × 106 Blast-EGFP and Blast-SPI1 cells were used, and ChIP analysis was performed using a Zymo-Spin ChIP Kit (D5210, Zymo Research). .. The antibodies used for the ChIP assays were anti-SPI1 (sc-352, Santa Cruz) and normal rabbit IgG (2729, Cell Signaling Technology). .. The enriched regions were quantified by qPCR using the primers described in .

    Sonication:

    Article Title: Atrophin controls developmental signaling pathways via interactions with Trithorax-like
    Article Snippet: .. ChIP-re-ChIP S2 cells were double crosslinked, sonicated, and ChIP’ed as above, using 5 μL anti-Atro sera (SG2524), and 10 μL normal rabbit IgG (Cell Signaling Technology). ..

    other:

    Article Title: SLC6A14, a Na+/Cl−-coupled amino acid transporter, functions as a tumor promoter in colon and is a target for Wnt signaling
    Article Snippet: Antibodies Anti-mTOR (#2983S), anti-P-mTOR (#5536S), anti-S6K (#9202S), anti-P-S6K (#9204S), anti-LC3A/B (#4108S) anti-β-catenin (#8814S), anti-Cyclin D1 (#2922S), anti-TCF4 (#2569S), and anti-IgG (#2729S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, U.S.A.).

    Article Title: Elmo2 Is a Regulator of Insulin-dependent Glut4 Membrane Translocation *
    Article Snippet: Mouse monoclonal anti-Elmo2, anti-Glu4, anti-Rac1, and anti-GST were from Santa Cruz Biotechnology, Inc. Rabbit monoclonal anti-phospho-AS160 at Thr-642, normal rabbit IgG, anti-AS160, anti-Akt, and phospho-Akt antibodies were from Cell Signaling.

    Immunoprecipitation:

    Article Title: TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice
    Article Snippet: Lysates were treated with RQ1 DNase (Promega) for 5 min at 37 °C and centrifuged at 90,000×g for 30 min at 4 °C. .. For each immunoprecipitation, TDRD5 antibody, MILI antibody (PM044, MBL), or Rabbit IgG (2729, Cell Signaling Technology) were bound on protein A Dynabeads (Life Technologies) in antibody binding buffer for 3 h at 4 °C. .. The beads were washed in antibody binding buffer, followed by 1X PMPG buffer, and incubated with lysates for 3 h at 4 °C.

    Article Title: BAP1 and YY1 regulate expression of death receptors in malignant pleural mesothelioma
    Article Snippet: Briefly, the cells were cross-linked, quenched and lysed then the chromatin was fragmented by sonication shearing. .. Protein/DNA complexes were diluted, pre-cleared with Protein G agarose beads, then immunoprecipitated (IP) by incubation with antibodies against BAP1 (Cell Signaling #78105), YY1 (Abcam #ab38422) or IgG (Cell Signaling #2729) overnight with rotation, followed by incubation with protein G agarose beads for 1 hr. .. After washing beads, protein/DNA complexes were eluted, reverse crosslinked to free DNA, which was then purified using spin columns and analysed by quantitative PCR (qPCR).

    Binding Assay:

    Article Title: TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice
    Article Snippet: Lysates were treated with RQ1 DNase (Promega) for 5 min at 37 °C and centrifuged at 90,000×g for 30 min at 4 °C. .. For each immunoprecipitation, TDRD5 antibody, MILI antibody (PM044, MBL), or Rabbit IgG (2729, Cell Signaling Technology) were bound on protein A Dynabeads (Life Technologies) in antibody binding buffer for 3 h at 4 °C. .. The beads were washed in antibody binding buffer, followed by 1X PMPG buffer, and incubated with lysates for 3 h at 4 °C.

    Incubation:

    Article Title: Squelching of ETS2 Transactivation by POU5F1 Silences the Human Chorionic Gonadotropin CGA Subunit Gene in Human Choriocarcinoma and Embryonic Stem Cells
    Article Snippet: Whole-cell lysate was prepared from undifferentiated H1 hESC (hESC/FGF2) with a buffer of 50 m m Tris-HCl (pH 8), 50 m m NaCl, 5 m m MgCl2 , 10% glycerol, 1% Nonidet P-40, 1 m m phenylmethylsulfonyl fluoride, protease inhibitor cocktail from Cell Signaling Technologies. .. Cell lysate containing 136 μg protein was incubated overnight with either 2 μg of anti-ETS2 antibody (sc-351; Santa Cruz Biotechnology) or normal rabbit IgG (Cell Signaling Technologies). .. Immune complexes were captured by the addition of a 50% slurry (40 μl) of protein G agarose beads (preadsorbed with BSA and sonicated salmon sperm DNA; Cell Signaling Technologies) and incubated for 24 h at 4 C. The beads were then washed three times in the buffer of 50 m m Tris-HCl (pH 8), 50 m m NaCl, 5 m m MgCl2 , 10% glycerol, 1% Nonidet P-40, 1 m m phenylmethylsulfonyl fluoride, protease inhibitor cocktail (Cell Signaling Technologies; 1 ml each).

    Article Title: BAP1 and YY1 regulate expression of death receptors in malignant pleural mesothelioma
    Article Snippet: Briefly, the cells were cross-linked, quenched and lysed then the chromatin was fragmented by sonication shearing. .. Protein/DNA complexes were diluted, pre-cleared with Protein G agarose beads, then immunoprecipitated (IP) by incubation with antibodies against BAP1 (Cell Signaling #78105), YY1 (Abcam #ab38422) or IgG (Cell Signaling #2729) overnight with rotation, followed by incubation with protein G agarose beads for 1 hr. .. After washing beads, protein/DNA complexes were eluted, reverse crosslinked to free DNA, which was then purified using spin columns and analysed by quantitative PCR (qPCR).

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  • 97
    Cell Signaling Technology Inc peroxidase conjugated goat anti rabbit igg
    IgE binding potency of Der f 34. A , IgE binding capacity of nDer f 34 in HDM-allergic patients. IgE binding frequencies and IgE levels to nDer f 34 in HDM-allergic patients are shown for individuals. ELISA was performed using plasma from 19 HDM-allergic patients and 4 healthy volunteers. The average + (3 × S.D.) of IgE titer from four healthy volunteers was set as a threshold value for positive results (2.8 units/ml), and that threshold value is indicated by a horizontal broken line . An average value with S.D. of nDer f 34-specific IgE titer for four healthy volunteers is labeled with H . An arbitrary unit ( AU ) is defined as an amount equivalent to 10 ng of human IgE. B , cross-reactivity of Der f 34 with allergenic fungal extract. The percentage inhibition of binding between rTF-Der f 34 and <t>IgG</t> specific to Der f 34 in anti-HM2 fraction <t>pAb</t> by rTF-Der f 34, D. farinae feces extract indicated as Mite extract , crude fungal extract from A. fumigatus or A. alternata , or rTF as a negative control is plotted on the y axis. The value for 0% inhibition was determined without inhibitor, and 100% was set as the saturated value for rTF-Der f 34 as a positive control. The data represent the average ± S.D. ( error bars ) for three individual experiments. Non-repeated ANOVA was applied to compare data among the groups and, when the differences were significant ( p
    Peroxidase Conjugated Goat Anti Rabbit Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti rabbit igg/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti rabbit igg - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc rabbit mab against akt
    Effects of LiCl on the DEX-induced inhibitory effect. Both wild-type HEK293 and HEK293/tau441 cells were pre-treated with LiCl (10 mM, 1 h) and then treated with DEX for 3 days (D3; A) or 6 days (D6; B), followed by stimulation of insulin and Western analysis of <t>pAkt</t> and <t>Akt.</t> Bars representing means ± SEM. Pre-treatment with LiCl prevented the inhibitory effect of DEX in HEK293/tau441 cells on D3.
    Rabbit Mab Against Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit mab against akt/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit mab against akt - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    IgE binding potency of Der f 34. A , IgE binding capacity of nDer f 34 in HDM-allergic patients. IgE binding frequencies and IgE levels to nDer f 34 in HDM-allergic patients are shown for individuals. ELISA was performed using plasma from 19 HDM-allergic patients and 4 healthy volunteers. The average + (3 × S.D.) of IgE titer from four healthy volunteers was set as a threshold value for positive results (2.8 units/ml), and that threshold value is indicated by a horizontal broken line . An average value with S.D. of nDer f 34-specific IgE titer for four healthy volunteers is labeled with H . An arbitrary unit ( AU ) is defined as an amount equivalent to 10 ng of human IgE. B , cross-reactivity of Der f 34 with allergenic fungal extract. The percentage inhibition of binding between rTF-Der f 34 and IgG specific to Der f 34 in anti-HM2 fraction pAb by rTF-Der f 34, D. farinae feces extract indicated as Mite extract , crude fungal extract from A. fumigatus or A. alternata , or rTF as a negative control is plotted on the y axis. The value for 0% inhibition was determined without inhibitor, and 100% was set as the saturated value for rTF-Der f 34 as a positive control. The data represent the average ± S.D. ( error bars ) for three individual experiments. Non-repeated ANOVA was applied to compare data among the groups and, when the differences were significant ( p

    Journal: The Journal of Biological Chemistry

    Article Title: Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases *

    doi: 10.1074/jbc.M116.728006

    Figure Lengend Snippet: IgE binding potency of Der f 34. A , IgE binding capacity of nDer f 34 in HDM-allergic patients. IgE binding frequencies and IgE levels to nDer f 34 in HDM-allergic patients are shown for individuals. ELISA was performed using plasma from 19 HDM-allergic patients and 4 healthy volunteers. The average + (3 × S.D.) of IgE titer from four healthy volunteers was set as a threshold value for positive results (2.8 units/ml), and that threshold value is indicated by a horizontal broken line . An average value with S.D. of nDer f 34-specific IgE titer for four healthy volunteers is labeled with H . An arbitrary unit ( AU ) is defined as an amount equivalent to 10 ng of human IgE. B , cross-reactivity of Der f 34 with allergenic fungal extract. The percentage inhibition of binding between rTF-Der f 34 and IgG specific to Der f 34 in anti-HM2 fraction pAb by rTF-Der f 34, D. farinae feces extract indicated as Mite extract , crude fungal extract from A. fumigatus or A. alternata , or rTF as a negative control is plotted on the y axis. The value for 0% inhibition was determined without inhibitor, and 100% was set as the saturated value for rTF-Der f 34 as a positive control. The data represent the average ± S.D. ( error bars ) for three individual experiments. Non-repeated ANOVA was applied to compare data among the groups and, when the differences were significant ( p

    Article Snippet: The two-dimensional immunoblotting analysis in the allergenomics study was carried out using plasma samples from 40 patients allergic to HDM.6 The HM2 fraction, the enriched nDer f 34 fraction, rDer f 34, and nDer f 34 were immunostained with 20,000-fold-diluted rabbit anti-rDer f 34 pAb, followed by 20,000-fold-diluted peroxidase-conjugated goat anti-rabbit IgG (Cell Signaling Technology, Danvers, MA), and visualized with ECL Plus Western blotting detection reagents (GE Healthcare Japan).

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Labeling, Inhibition, Negative Control, Positive Control

    Effects of LiCl on the DEX-induced inhibitory effect. Both wild-type HEK293 and HEK293/tau441 cells were pre-treated with LiCl (10 mM, 1 h) and then treated with DEX for 3 days (D3; A) or 6 days (D6; B), followed by stimulation of insulin and Western analysis of pAkt and Akt. Bars representing means ± SEM. Pre-treatment with LiCl prevented the inhibitory effect of DEX in HEK293/tau441 cells on D3.

    Journal: PLoS ONE

    Article Title: Tau Phosphorylation and ?-Calpain Activation Mediate the Dexamethasone-Induced Inhibition on the Insulin-Stimulated Akt Phosphorylation

    doi: 10.1371/journal.pone.0035783

    Figure Lengend Snippet: Effects of LiCl on the DEX-induced inhibitory effect. Both wild-type HEK293 and HEK293/tau441 cells were pre-treated with LiCl (10 mM, 1 h) and then treated with DEX for 3 days (D3; A) or 6 days (D6; B), followed by stimulation of insulin and Western analysis of pAkt and Akt. Bars representing means ± SEM. Pre-treatment with LiCl prevented the inhibitory effect of DEX in HEK293/tau441 cells on D3.

    Article Snippet: Rabbit mAb against Akt and phosphorylated Akt at Ser473 (pAkt) were obtained from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Western Blot

    Effects of dexamethasone on the insulin-stimulated Akt phosphorylation. A): Both wild-type HEK293 and HEK293/tau441 cells were treated with 1 µM dexamethasone (DEX) for 1–6 days and then stimulated with 100 nM insulin (i) for 15 min. Representative immunoblots from the results on the third day (D3) and the sixth day (D6) after DEX treatment were shown. Bars representing means ± SEM were shown below. Total amounts of Akt remained stable under the conditions. In HEK293/tau441 cells, DEX prevented the insulin-stimulated increases in pAkt on D3 and D6. In wild-type HEK293 cells, the inhibitory effect of DEX was evident on D6 but not on D3. Each experiment was repeated three times unless stated otherwise. * P

    Journal: PLoS ONE

    Article Title: Tau Phosphorylation and ?-Calpain Activation Mediate the Dexamethasone-Induced Inhibition on the Insulin-Stimulated Akt Phosphorylation

    doi: 10.1371/journal.pone.0035783

    Figure Lengend Snippet: Effects of dexamethasone on the insulin-stimulated Akt phosphorylation. A): Both wild-type HEK293 and HEK293/tau441 cells were treated with 1 µM dexamethasone (DEX) for 1–6 days and then stimulated with 100 nM insulin (i) for 15 min. Representative immunoblots from the results on the third day (D3) and the sixth day (D6) after DEX treatment were shown. Bars representing means ± SEM were shown below. Total amounts of Akt remained stable under the conditions. In HEK293/tau441 cells, DEX prevented the insulin-stimulated increases in pAkt on D3 and D6. In wild-type HEK293 cells, the inhibitory effect of DEX was evident on D6 but not on D3. Each experiment was repeated three times unless stated otherwise. * P

    Article Snippet: Rabbit mAb against Akt and phosphorylated Akt at Ser473 (pAkt) were obtained from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Western Blot

    Schematic diagram of the proposed role of miR-34a in PA-induced cholangiocyte lipoapoptosis. The saturated FFA, PA, increased nuclear FoxO3 levels leading to increased miR-34a in PA-treated cells. miR-34a then promoted lipoapoptosis, likely through decreasing protein levels of its targets, SIRT1, MET, and KLF4. Based on literature reports of SIRT1 activity on FoxO3 and our previous finding of increased nuclear acetylated-FoxO3, we speculate that a miR-34a-mediated decrease in SIRT1 may enhance FoxO3 acetylation levels.

    Journal: Journal of Lipid Research

    Article Title: FoxO3 increases miR-34a to cause palmitate-induced cholangiocyte lipoapoptosis

    doi: 10.1194/jlr.M071357

    Figure Lengend Snippet: Schematic diagram of the proposed role of miR-34a in PA-induced cholangiocyte lipoapoptosis. The saturated FFA, PA, increased nuclear FoxO3 levels leading to increased miR-34a in PA-treated cells. miR-34a then promoted lipoapoptosis, likely through decreasing protein levels of its targets, SIRT1, MET, and KLF4. Based on literature reports of SIRT1 activity on FoxO3 and our previous finding of increased nuclear acetylated-FoxO3, we speculate that a miR-34a-mediated decrease in SIRT1 may enhance FoxO3 acetylation levels.

    Article Snippet: Antibodies Rabbit antiserum against FoxO3 (2497), cleaved caspase 3 (C9661), and cleaved poly (ADP-ribose) polymerase ( PARP) (P9542) were from Cell Signaling.

    Techniques: Activity Assay

    FoxO3 induced miR-34a expression. A: Nuclear extracts were prepared from H69 or KMCH cells treated with vehicle (Veh) or PA for 24 h. FoxO3 and lamin B were detected by immunoblot. B, C: Cell lines (H69 or KMCH) were transduced with shRNA to GFP or FoxO3 (two shRNAs, indicated as #1 or #2) and stably-transfected cells selected by antibiotic resistance. Whole-cell FoxO3 protein levels were compared with actin. D, E: GFP shRNA- or FoxO3 shRNA-transduced cells were treated with PA (600 μM; filled bars) or vehicle (Veh; open bars) for 24 h. miR-34a levels were normalized to Z30. Data represent the mean ± SEM for n = 4. * P

    Journal: Journal of Lipid Research

    Article Title: FoxO3 increases miR-34a to cause palmitate-induced cholangiocyte lipoapoptosis

    doi: 10.1194/jlr.M071357

    Figure Lengend Snippet: FoxO3 induced miR-34a expression. A: Nuclear extracts were prepared from H69 or KMCH cells treated with vehicle (Veh) or PA for 24 h. FoxO3 and lamin B were detected by immunoblot. B, C: Cell lines (H69 or KMCH) were transduced with shRNA to GFP or FoxO3 (two shRNAs, indicated as #1 or #2) and stably-transfected cells selected by antibiotic resistance. Whole-cell FoxO3 protein levels were compared with actin. D, E: GFP shRNA- or FoxO3 shRNA-transduced cells were treated with PA (600 μM; filled bars) or vehicle (Veh; open bars) for 24 h. miR-34a levels were normalized to Z30. Data represent the mean ± SEM for n = 4. * P

    Article Snippet: Antibodies Rabbit antiserum against FoxO3 (2497), cleaved caspase 3 (C9661), and cleaved poly (ADP-ribose) polymerase ( PARP) (P9542) were from Cell Signaling.

    Techniques: Expressing, Transduction, shRNA, Stable Transfection, Transfection

    Allicin (300 mg/kg) inhibited the reticuloendotheliosis virus (REV) replication in lymphocytes isolated from spleens by reducing the phosphorylation of ERK. (A) Western blot analysis of mitogen-activated protein kinase and nuclear factor kappa B (NF-κB). (B) REV replication (folds of the expression of pol in 5 days). (C,D) Effect of P38 inhibitor on the expression of p38 and REV pol (folds of the expression of pol in 0 µM group). (E,F) Effect of JNK inhibitor on the expression of JNK and REV pol (folds of the expression of pol in 0 µM group). (G,H) Effect of ERK inhibitor on ERK and REV pol (folds of the expression of pol in 0 µM group). (I) Western blot analysis of REV and ERK activator (Ceramide C6) on ERK. (J,K) Effect of Ceramide C6 and allicin on REV replication (folds of the expression of pol and LTR in REV group). All data are presented as mean ± SE ( n = 6). “*” denotes a statistically significant difference at P

    Journal: Frontiers in Immunology

    Article Title: Allicin Alleviates Reticuloendotheliosis Virus-Induced Immunosuppression via ERK/Mitogen-Activated Protein Kinase Pathway in Specific Pathogen-Free Chickens

    doi: 10.3389/fimmu.2017.01856

    Figure Lengend Snippet: Allicin (300 mg/kg) inhibited the reticuloendotheliosis virus (REV) replication in lymphocytes isolated from spleens by reducing the phosphorylation of ERK. (A) Western blot analysis of mitogen-activated protein kinase and nuclear factor kappa B (NF-κB). (B) REV replication (folds of the expression of pol in 5 days). (C,D) Effect of P38 inhibitor on the expression of p38 and REV pol (folds of the expression of pol in 0 µM group). (E,F) Effect of JNK inhibitor on the expression of JNK and REV pol (folds of the expression of pol in 0 µM group). (G,H) Effect of ERK inhibitor on ERK and REV pol (folds of the expression of pol in 0 µM group). (I) Western blot analysis of REV and ERK activator (Ceramide C6) on ERK. (J,K) Effect of Ceramide C6 and allicin on REV replication (folds of the expression of pol and LTR in REV group). All data are presented as mean ± SE ( n = 6). “*” denotes a statistically significant difference at P

    Article Snippet: The membranes were blocked with blocking buffer (Beyotime) at room temperature for 2 h and then were incubated with anti-phospho-P38 [#4511T, anti-rabbit, Cell Signaling Technology (CST), USA], anti-P38 (#9212S, anti-rabbit, CST), anti-phospho-JNK (#4668S, anti-rabbit, CST), anti-JNK (#928, anti-rabbit, CST), anti-phospho-ERK (#9101S, anti-rabbit, CST), anti-ERK (#9102S, anti-rabbit, CST), anti-TLR-3 (NBP2-24565, anti-rabbit, NBP2-24565Novus Biologicals, USA), and anti-TLR-4 (BA1717, anti-rabbit, Boster, China) primary antibodies overnight at 4°C, followed by incubation with the corresponding horseradish peroxidase-conjugated secondary antibody (Beyotime) at 4°C for 4 h. The protein–antibody complexes were detected with the ECL Plus A and B (Beyotime), and the results were quantified using the Fusion FX software (Vilber, France).

    Techniques: Isolation, Western Blot, Expressing