anti rabbit igg cy3  (Jackson Immuno)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Fluorescein FITC AffiniPure Donkey Anti Mouse IgG
    Description:
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine chicken goat guinea pig syrian hamster horse human rabbit and sheep serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    715-095-150
    Price:
    101.0
    Category:
    Whole IgG Affinity Purified Antibodies
    Conjugate:
    Fluorescein FITC
    Size:
    0 5 mg
    Format:
    Whole IgG
    Host:
    Donkey
    Buy from Supplier


    Structured Review

    Jackson Immuno anti rabbit igg cy3
    Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent The average molecular weight is reported to be about 160 kDa The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective Based on immunoelectrophoresis and or ELISA the antibody reacts with whole molecule mouse IgG It also reacts with the light chains of other mouse immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with bovine chicken goat guinea pig syrian hamster horse human rabbit and sheep serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/anti rabbit igg cy3/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti rabbit igg cy3 - by Bioz Stars, 2021-09
    99/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells
    Article Snippet: .. Then, cells were rinsed with PBS, fixed in 4% formalin (10 min), washed 3× by PBS, and permeabilized with 0.5% Triton X-100 in PBS for 5 min. After, cells were washed with PBS 3 × 5 min and blocked with 1% bovine serum albumin (BSA, Sigma-Aldrich, Poland, Poznan) in PBS for 1 h. The following antibodies were used: primary antibody monoclonal anti-MDR1 (1:200; Santa Cruz, USA) for overnight incubation at 4 °C, and secondary antibody Fluorescein (FITC)-conjugated AffiniPure Fragment Donkey Anti-Mouse IgG (cat. no.: 715-095-150), where the incubation was for 60 min, at RT (1:100; Jackson ImmunoResearch Laboratories, Biokom, Poland, Wroclaw). ..

    Article Title: Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy
    Article Snippet: .. After 3x 5 min washing steps in PBS, cover slips were incubated with Cy3 donkey anti-rabbit (Jackson Immuno Research, 711-165-152, 1:100) or FITC donkey anti-mouse (Jackson Immuno Research, 715-095-150, 1:100) secondary antibodies in 1% BSA in 0.1% PBS-T. ..

    Article Title: Combination treatment with U0126 and rt-PA prevents adverse effects of the delayed rt-PA treatment after acute ischemic stroke
    Article Snippet: .. The sections were subsequently incubated for 1 h at room temperature with FITC-conjugated donkey anti-mouse (1:100, Jackson ImmunoResearch) or FITC-conjugated goat anti-rabbit (1:100, Jackson ImmunoResearch) as secondary antibody. ..

    Article Title: Low dose/dose rate γ irradiation protects Drosophila melanogaster chromosomes from double strand breaks and telomere fusions by modulating the expression of Loquacious
    Article Snippet: .. Then, all slides were incubated with the fluorescein (FITC)-conjugated AffiniPure Donkey Anti-Mouse IgG (H+L) (1:150; Jackson ImmunoResearch) for 1 hour at room temperature (RT). ..

    Article Title: Wnt-Signaling Inhibitor Wnt-C59 Suppresses the Cytokine Upregulation in Multiple Organs of Lipopolysaccharide-Induced Endotoxemic Mice via Reducing the Interaction between β-Catenin and NF-κB
    Article Snippet: .. Next, the sections were washed three times with PBS containing 0.03% Triton X-100 and then incubated with Cy3-labeled donkey anti-rabbit IgG (1:400; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) and FITC-labeled donkey anti-mouse IgG (1:400; Jackson ImmunoResearch Laboratories) antibodies in PBS at 25 °C for 1 h. After counterstaining with 1 μg/mL of DAPI (Sigma-Aldrich), the sections were examined using a confocal microscope (Nikon, Tokyo, Japan). ..

    other:

    Article Title: Regulation of layered T cell tolerance mechanisms by the NR4A family is essential to preserve immune homeostasis and suppress autoimmunity
    Article Snippet: Anti-nuclear antibody (ANA) NOVA LiteTM HEp-2 ANA Substrate Slide and mounting medium (INOVA Diagnostics, Inc, #708100); FITC donkey anti-mouse IgG (Jackson ImmunoResearch, #715-095-150).

    Microscopy:

    Article Title: Wnt-Signaling Inhibitor Wnt-C59 Suppresses the Cytokine Upregulation in Multiple Organs of Lipopolysaccharide-Induced Endotoxemic Mice via Reducing the Interaction between β-Catenin and NF-κB
    Article Snippet: .. Next, the sections were washed three times with PBS containing 0.03% Triton X-100 and then incubated with Cy3-labeled donkey anti-rabbit IgG (1:400; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA) and FITC-labeled donkey anti-mouse IgG (1:400; Jackson ImmunoResearch Laboratories) antibodies in PBS at 25 °C for 1 h. After counterstaining with 1 μg/mL of DAPI (Sigma-Aldrich), the sections were examined using a confocal microscope (Nikon, Tokyo, Japan). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Jackson Immuno cy3 goat anti rabbit ab
    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat <t>Cy3</t> <t>anti-rabbit</t> Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).
    Cy3 Goat Anti Rabbit Ab, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 goat anti rabbit ab/product/Jackson Immuno
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 goat anti rabbit ab - by Bioz Stars, 2021-09
    96/100 stars
      Buy from Supplier

    99
    Jackson Immuno cy3 conjugated goat anti rabbit igg
    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit <t>IgG</t> (green) and mono-clonal rat antibody against mouse CD68 followed by <t>Cy3-con-jugated</t> anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.
    Cy3 Conjugated Goat Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated goat anti rabbit igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated goat anti rabbit igg - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    99
    Jackson Immuno cy3 conjugated donkey anti rabbit igg
    Cellugyrin with the C-terminus of GLUT4 (CG) co-localizes with GLUT4 and syntaxin 6 in the perinuclear compartment ( A ) Immunofluorescence analysis of 3T3-L1 adipocytes stably expressing EGFP–cellugyrin or EGFP–CG. ( B ) 3T3-L1 adipocytes stably expressing EGFP–CG were electroporated with the cDNA for Myc–GLUT4. Cells were re-plated, grown overnight, then fixed, permeabilized and stained with monoclonal anti-Myc antibody followed by <t>Cy3-conjugated</t> donkey anti-mouse <t>IgG.</t> ( C ) 3T3-L1 adipocytes stably expressing GFP–CG were fixed, permeabilized and stained with a monoclonal antibody against syntaxin 6 followed by Cy3-conjugated donkey anti-mouse IgG.
    Cy3 Conjugated Donkey Anti Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy3 conjugated donkey anti rabbit igg/product/Jackson Immuno
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy3 conjugated donkey anti rabbit igg - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Journal: The Journal of Cell Biology

    Article Title: Regulated Targeting of BAX to Mitochondria

    doi:

    Figure Lengend Snippet: Subcellular distribution of BAX. BAX immunoreactivity localizes to mitochondria, perinuclear membrane, and cytosol in FL5.12 cells. Immunofluorescent confocal microscopy of FL5.12 cells using rabbit anti-BAX (P-19) antibody and goat Cy3 anti-rabbit Ig antibody shows both punctate and diffuse cytoplasmic immunoreactivity ( A ). MitoTracker Green FM shows exclusive punctate labeling ( B ). Dual exposure of BAX and MitoTracker labeling reveals colocalization of BAX with mitochondria ( yellow ) as well as nonmitochondrial BAX immunoreactivity ( red ; C ). Conventional fluorescence detection of anti-BAX (P-19) immunoreactivity (Cy3) and Hoechst H33258 nuclear stain shows red cytoplasmic BAX immunoreactivity, blue nuclear Hoechst staining and pink perinuclear signal where the two reactivities overlap ( D ).

    Article Snippet: Anti-mBAX (P-19) Ab ( Santa Cruz Biotechnology , Santa Cruz, CA) and Cy3 goat anti–rabbit Ab (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) were used to detect BAX immunoreactivity.

    Techniques: Confocal Microscopy, Labeling, Fluorescence, Staining

    Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: TLR4 mediates LPS-induced HO-1 expression in mouse liver: Role of TNF-α and IL-1β

    doi: 10.3748/wjg.v9.i8.1799

    Figure Lengend Snippet: Double immunofluorescent staining of HO-1 local-ization in liver. After three doses of LPS treatment, HO-1 and CD68 in liver tissue were detected by double immunofluores-cent staining with polyclonal rabbit antibody against HO-1 followed by FITC-conjugated anti-rabbit IgG (green) and mono-clonal rat antibody against mouse CD68 followed by Cy3-con-jugated anti-rat IgG (red). Cell nuclei were counterstained with bis -benzimide (blue). A: HO-1; B: Macrophages (Kupffer cells); C: HO-1 + Macrophages. magnification × 400.

    Article Snippet: Rat IgG and Cy3-conjugated goat anti-rabbit IgG were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA).

    Techniques: Staining

    Glut4 is translocated to the cell surface of cerebellar neurons in response to insulin stimulation A: Primary cultures of cerebellum neurons were serum-starved for 2h and treated or not treated with 100 nM insulin for 15 min. Top panels: cells were stained with the polyclonal antibody MC2A against Glut4 followed by Cy3-conjugated donkey anti-rabbit IgG. Bottom panels: phase-contrast images. B: Primary cultures of cerebellar neurons were serum starved for 2 h and treated as indicated with Indinavir (100 nM) for 4 hrs, Wortmannin (100 nM) and Cytochalasin B (5 μM) for 30 min, and Insulin (100 nM) for 15 min. Glucose uptake was measured in cultured cells in triplicate. A representative result of three independent experiments is shown.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Cerebellar neurons possess a vesicular compartment structurally and functionally similar to Glut4-storage vesicles from peripheral insulin-sensitive tissues

    doi: 10.1523/JNEUROSCI.0858-09.2009

    Figure Lengend Snippet: Glut4 is translocated to the cell surface of cerebellar neurons in response to insulin stimulation A: Primary cultures of cerebellum neurons were serum-starved for 2h and treated or not treated with 100 nM insulin for 15 min. Top panels: cells were stained with the polyclonal antibody MC2A against Glut4 followed by Cy3-conjugated donkey anti-rabbit IgG. Bottom panels: phase-contrast images. B: Primary cultures of cerebellar neurons were serum starved for 2 h and treated as indicated with Indinavir (100 nM) for 4 hrs, Wortmannin (100 nM) and Cytochalasin B (5 μM) for 30 min, and Insulin (100 nM) for 15 min. Glucose uptake was measured in cultured cells in triplicate. A representative result of three independent experiments is shown.

    Article Snippet: Sections were blocked with MOM mouse IgG blocking reagent (Vector Laboratories), washed with Gadenza buffer (Vector Laboratories) and stained with polyclonal anti-Glut4 antibody MC2A followed by Cy3-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch).

    Techniques: Staining, Cell Culture

    Cellugyrin with the C-terminus of GLUT4 (CG) co-localizes with GLUT4 and syntaxin 6 in the perinuclear compartment ( A ) Immunofluorescence analysis of 3T3-L1 adipocytes stably expressing EGFP–cellugyrin or EGFP–CG. ( B ) 3T3-L1 adipocytes stably expressing EGFP–CG were electroporated with the cDNA for Myc–GLUT4. Cells were re-plated, grown overnight, then fixed, permeabilized and stained with monoclonal anti-Myc antibody followed by Cy3-conjugated donkey anti-mouse IgG. ( C ) 3T3-L1 adipocytes stably expressing GFP–CG were fixed, permeabilized and stained with a monoclonal antibody against syntaxin 6 followed by Cy3-conjugated donkey anti-mouse IgG.

    Journal: The Biochemical journal

    Article Title: The C-terminus of GLUT4 targets the transporter to the perinuclear compartment but not to the insulin-responsive vesicles

    doi: 10.1042/BJ20081448

    Figure Lengend Snippet: Cellugyrin with the C-terminus of GLUT4 (CG) co-localizes with GLUT4 and syntaxin 6 in the perinuclear compartment ( A ) Immunofluorescence analysis of 3T3-L1 adipocytes stably expressing EGFP–cellugyrin or EGFP–CG. ( B ) 3T3-L1 adipocytes stably expressing EGFP–CG were electroporated with the cDNA for Myc–GLUT4. Cells were re-plated, grown overnight, then fixed, permeabilized and stained with monoclonal anti-Myc antibody followed by Cy3-conjugated donkey anti-mouse IgG. ( C ) 3T3-L1 adipocytes stably expressing GFP–CG were fixed, permeabilized and stained with a monoclonal antibody against syntaxin 6 followed by Cy3-conjugated donkey anti-mouse IgG.

    Article Snippet: Cy3 (indocarbocyanine)-conjugated donkey anti-mouse IgG and Cy3-conjugated donkey anti-rabbit IgG were purchased from Jackson ImmunoResearch Laboratories.

    Techniques: Immunofluorescence, Stable Transfection, Expressing, Staining