Journal: eLife

Article Title: Spinal V1 inhibitory interneuron clades differ in birthdate, projections to motoneurons, and heterogeneity

doi: 10.7554/eLife.95172

Figure Lengend Snippet: Antibodies.

Article Snippet: Prox1 , Synthetic peptide from the C-terminus of mouse Prox1 , Rabbit, polyclonal , Millipore Sigma , AB5475 , RRID: AB_177485 , 1:500.

Techniques: Recombinant, Variant Assay, Isolation

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility

doi: 10.1007/s00018-024-05481-z

Figure Lengend Snippet: Immunofluorescence analyses on differentiated MNCdLS1 and G12 iPSCs Immunofluorescence analyses were performed on differentiated MNCdLS1 and G12 iPSC s at day 9 and day 22, as indicated. Fluorescent images were captured under the microscope (Magnification × 200). We used the following antibodies: anti-FOXA2 (Invitrogen 701,698), anti-PROX1 (Invitrogen PA5-85,552), anti-HNF4A (Invitrogen MA1-199), anti-AFP (anti-AFPα; Invitrogen PA5-16,658) and anti-ALB (anti-albumin; Invitrogen PA5-111,010). a Representative immunofluorescence images obtained with anti-FOXA2 and anti-PROX1 antibodies are illustrated. b Representative immunofluorescence images obtained with combinations of anti-HNF4A and anti-AFP or combinations of anti-HNF4A and anti-ALB antibodies are illustrated. The number of HNF4A positive cells (magenta-fluorescence) and the number of nuclei (blu-fluorescence; DAPI) were counted in 10 separate fields (approximately 1000 cells/field) and the percentage of HNF4A positive nuclei relative to the total number of nuclei was calculated (upper right bar-graph). The percentage of HNF4A positive cells (magenta-fluorescence) in AFP positive cells (green-fluorescence) is illustrated by the middle right graph. The percentage of HNF4A positive cells (magenta-fluorescence) in ALB positive cells (green-fluorescence) is shown in the bottom right graph. ** Significantly lower in MNCdLS1 relative to G12 cell cultures ( p < 0.01; unpaired t-test). c CYP3A4 enzymatic activity was measured in hepatocyte differentiated cells at D25 of differentiation with or without induction with dexamethasone (50 µM) for 24 h. Following exposure of cells to the luciferin substrate for 1 h, cell supernatants were used for CYP3A4 enzymatic assays. In parallel cells were detached from the plates and the number of hepatocytes (30–40 µm in diameter) was determined and used for the normalization of the results. ** Significantly lower in MNCdLS1 relative to G12 cell cultures ( p < 0.01; unpaired t-test)

Article Snippet: We used the following antibodies: anti-FOXA2 (Invitrogen 701,698), anti-PROX1 (Invitrogen PA5-85,552), anti-HNF4A (Invitrogen MA1-199), anti-AFP (anti-AFPα; Invitrogen PA5-16,658) and anti-ALB (anti-albumin; Invitrogen PA5-111,010). a Representative immunofluorescence images obtained with anti-FOXA2 and anti-PROX1 antibodies are illustrated. b Representative immunofluorescence images obtained with combinations of anti-HNF4A and anti-AFP or combinations of anti-HNF4A and anti-ALB antibodies are illustrated.

Techniques: Immunofluorescence, Microscopy, Fluorescence, Activity Assay