mouse anti prox1  (Novus Biologicals)


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    Novus Biologicals mouse anti prox1
    A) Diffusion maps of rat intestinal epithelial cells, showing their differentiation as a measure of pseudotime. E1-E6 denote enterocytes of increasing maturation. B) Reclustered UMAP of cells arising from stem and secretory progenitor clusters. C) Diffusion maps of stem cells and secretory progenitors as a readout of pseudotime. Note the clear bifurcation between Goblet/Paneth and the EEC/Tuft/CHE lineages. D) Diffusion map color coded for Atoh1 expression. E) Atoh1 (magenta) and Goblet cell staining (Muc2; cyan). F) Atoh1 (magenta) and Best4 (cyan; CHE marker) costaining. G) Atoh1 (magenta) and Dclk1 (cyan; Tuft cell marker) costaining. H) Diffusion map of stem and secretory cells color coded for <t>Prox1</t> expression. I) CFTR (cyan; CHE marker) and Prox1 (magenta) co-staining. J) ChgA (cyan; EEC marker) and Prox1 (magenta) costaining. Arrow marks Prox1+/ChgA+ cell, red arrowhead marks Prox1-/ChgA+ cell. K-K’) Dclk1 (cyan, Tuft cell marker) and Prox1 costaining (in magenta, K’). L) Proportion of Tuft cells and EECs in rat proximal jejunum that are Prox1+. n=2 animals. Error bars, SD. M) Diffusion map of stem and secretory cells color-coded for Meis1 expression. N) Meis1 (magenta) and CFTR (cyan, CHE marker) costaining. DAPI in white. O-Q) Meis1 (magenta) staining with O) Muc2 (cyan, Goblet cells), P) Dclk1 (cyan, Tuft cells) or Q) ChgA (cyan, EECs). All scale bars, 10 μm.
    Mouse Anti Prox1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti prox1/product/Novus Biologicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse anti prox1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "A second wave of Notch signaling diversifies the intestinal secretory lineage"

    Article Title: A second wave of Notch signaling diversifies the intestinal secretory lineage

    Journal: bioRxiv

    doi: 10.1101/2024.07.15.603542

    A) Diffusion maps of rat intestinal epithelial cells, showing their differentiation as a measure of pseudotime. E1-E6 denote enterocytes of increasing maturation. B) Reclustered UMAP of cells arising from stem and secretory progenitor clusters. C) Diffusion maps of stem cells and secretory progenitors as a readout of pseudotime. Note the clear bifurcation between Goblet/Paneth and the EEC/Tuft/CHE lineages. D) Diffusion map color coded for Atoh1 expression. E) Atoh1 (magenta) and Goblet cell staining (Muc2; cyan). F) Atoh1 (magenta) and Best4 (cyan; CHE marker) costaining. G) Atoh1 (magenta) and Dclk1 (cyan; Tuft cell marker) costaining. H) Diffusion map of stem and secretory cells color coded for Prox1 expression. I) CFTR (cyan; CHE marker) and Prox1 (magenta) co-staining. J) ChgA (cyan; EEC marker) and Prox1 (magenta) costaining. Arrow marks Prox1+/ChgA+ cell, red arrowhead marks Prox1-/ChgA+ cell. K-K’) Dclk1 (cyan, Tuft cell marker) and Prox1 costaining (in magenta, K’). L) Proportion of Tuft cells and EECs in rat proximal jejunum that are Prox1+. n=2 animals. Error bars, SD. M) Diffusion map of stem and secretory cells color-coded for Meis1 expression. N) Meis1 (magenta) and CFTR (cyan, CHE marker) costaining. DAPI in white. O-Q) Meis1 (magenta) staining with O) Muc2 (cyan, Goblet cells), P) Dclk1 (cyan, Tuft cells) or Q) ChgA (cyan, EECs). All scale bars, 10 μm.
    Figure Legend Snippet: A) Diffusion maps of rat intestinal epithelial cells, showing their differentiation as a measure of pseudotime. E1-E6 denote enterocytes of increasing maturation. B) Reclustered UMAP of cells arising from stem and secretory progenitor clusters. C) Diffusion maps of stem cells and secretory progenitors as a readout of pseudotime. Note the clear bifurcation between Goblet/Paneth and the EEC/Tuft/CHE lineages. D) Diffusion map color coded for Atoh1 expression. E) Atoh1 (magenta) and Goblet cell staining (Muc2; cyan). F) Atoh1 (magenta) and Best4 (cyan; CHE marker) costaining. G) Atoh1 (magenta) and Dclk1 (cyan; Tuft cell marker) costaining. H) Diffusion map of stem and secretory cells color coded for Prox1 expression. I) CFTR (cyan; CHE marker) and Prox1 (magenta) co-staining. J) ChgA (cyan; EEC marker) and Prox1 (magenta) costaining. Arrow marks Prox1+/ChgA+ cell, red arrowhead marks Prox1-/ChgA+ cell. K-K’) Dclk1 (cyan, Tuft cell marker) and Prox1 costaining (in magenta, K’). L) Proportion of Tuft cells and EECs in rat proximal jejunum that are Prox1+. n=2 animals. Error bars, SD. M) Diffusion map of stem and secretory cells color-coded for Meis1 expression. N) Meis1 (magenta) and CFTR (cyan, CHE marker) costaining. DAPI in white. O-Q) Meis1 (magenta) staining with O) Muc2 (cyan, Goblet cells), P) Dclk1 (cyan, Tuft cells) or Q) ChgA (cyan, EECs). All scale bars, 10 μm.

    Techniques Used: Diffusion-based Assay, Expressing, Staining, Marker

    A) Dotplot from scRNA-seq data showing relative expression of factors associated with Notch signaling across intestinal epithelial cell types. B-C) Module scores for Notch-on (B) and Notch-off (C) gene signatures. D) Meis1 (magenta, CHEs) and Notch2 receptor (cyan) costaining. DAPI in white. E) Rat proximal jejunum stained for Best4 (cyan), Prox1 (yellow), and Notch2 (magenta). E’) triple positive CHEs in the differentiated villus region, E’’-E’’’) Notch2+/Prox1+ cells in crypts that do not express Best4. Color of insert box denotes approximate localization along the crypt-villus axis (left). DAPI in white. F) Rat proximal jejunum stained for Notch2 (magenta) and Meis1 (yellow) with CFTR (cyan). Color of insert box denotes approximate localization along the crypt-villus axis (left; F’-F”’). In F”’, magenta asterisk denotes a stromal non-epithelial expressing Meis1. G-G”) Notch2+ /Prox1+ cell expressing Ki67. Notch2 in yellow, Prox1 in magenta, Ki67 in cyan. H-H’) Notch2+/Prox1+ crypt cells in active mitosis. Note symmetric cell division for both Notch2 (yellow) and Prox1 (magenta). H’) Low level of phospho-histone 3 (in cyan) as cell is exiting mitosis. I-I’) Notch2+/Meis1 Low cells in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. J-J’) Notch2+ cell lacking Meis1 in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. All scale bars, 10 μm.
    Figure Legend Snippet: A) Dotplot from scRNA-seq data showing relative expression of factors associated with Notch signaling across intestinal epithelial cell types. B-C) Module scores for Notch-on (B) and Notch-off (C) gene signatures. D) Meis1 (magenta, CHEs) and Notch2 receptor (cyan) costaining. DAPI in white. E) Rat proximal jejunum stained for Best4 (cyan), Prox1 (yellow), and Notch2 (magenta). E’) triple positive CHEs in the differentiated villus region, E’’-E’’’) Notch2+/Prox1+ cells in crypts that do not express Best4. Color of insert box denotes approximate localization along the crypt-villus axis (left). DAPI in white. F) Rat proximal jejunum stained for Notch2 (magenta) and Meis1 (yellow) with CFTR (cyan). Color of insert box denotes approximate localization along the crypt-villus axis (left; F’-F”’). In F”’, magenta asterisk denotes a stromal non-epithelial expressing Meis1. G-G”) Notch2+ /Prox1+ cell expressing Ki67. Notch2 in yellow, Prox1 in magenta, Ki67 in cyan. H-H’) Notch2+/Prox1+ crypt cells in active mitosis. Note symmetric cell division for both Notch2 (yellow) and Prox1 (magenta). H’) Low level of phospho-histone 3 (in cyan) as cell is exiting mitosis. I-I’) Notch2+/Meis1 Low cells in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. J-J’) Notch2+ cell lacking Meis1 in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. All scale bars, 10 μm.

    Techniques Used: Expressing, Staining

    Working model of CHE cell differentiation in which: 1) CHEs arise from the secretory lineage, which is initially Notch off, 2) Notch is reactivated along the secretory lineage as a potentially multipotent Notch2+/Prox1+ progenitor and 3) Meis1 is upregulated to promote the expression of differentiated CHE markers, including CFTR and Best4.
    Figure Legend Snippet: Working model of CHE cell differentiation in which: 1) CHEs arise from the secretory lineage, which is initially Notch off, 2) Notch is reactivated along the secretory lineage as a potentially multipotent Notch2+/Prox1+ progenitor and 3) Meis1 is upregulated to promote the expression of differentiated CHE markers, including CFTR and Best4.

    Techniques Used: Cell Differentiation, Expressing

    prox1  (R&D Systems)


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    R&D Systems prox1
    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, <t>Prox1,</t> and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prox1/product/R&D Systems
    Average 86 stars, based on 1 article reviews
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    prox1 - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Ventricular lymphatic vessel density correlates with mild cardiac remodelling and preserved heart function in moderate murine aortic valve stenosis"

    Article Title: Ventricular lymphatic vessel density correlates with mild cardiac remodelling and preserved heart function in moderate murine aortic valve stenosis

    Journal: bioRxiv

    doi: 10.1101/2024.07.06.602334

    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, Prox1, and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Figure Legend Snippet: Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, Prox1, and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.

    Techniques Used: Marker, Staining, MANN-WHITNEY

    monoclonal mouse prox 1  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
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    Thermo Fisher monoclonal mouse prox 1
    Monoclonal Mouse Prox 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    monoclonal mouse prox 1 - by Bioz Stars, 2024-07
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    Structured Review

    Reliatech anti prox1 antibody
    Anti Prox1 Antibody, supplied by Reliatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti prox1 antibody/product/Reliatech
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    anti prox1  (R&D Systems)


    Bioz Verified Symbol R&D Systems is a verified supplier
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    R&D Systems anti prox1
    Anti Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti prox1/product/R&D Systems
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    anti prox1 - by Bioz Stars, 2024-07
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    Structured Review

    Millipore anti prox1
    Anti Prox1, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti prox1 - by Bioz Stars, 2024-07
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    rabbit anti prox1  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
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    Danaher Inc rabbit anti prox1
    (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) <t>Prox1-</t> and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone
    Rabbit Anti Prox1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti prox1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
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    rabbit anti prox1 - by Bioz Stars, 2024-07
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    Images

    1) Product Images from "Single-Cell and Spatial Transcriptomic Analyses Reveals the Dynamic Transcript Profiles of Myocardial Lymphangiogenesis post Myocardial Infarction"

    Article Title: Single-Cell and Spatial Transcriptomic Analyses Reveals the Dynamic Transcript Profiles of Myocardial Lymphangiogenesis post Myocardial Infarction

    Journal: bioRxiv

    doi: 10.1101/2024.06.10.598235

    (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) Prox1- and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone
    Figure Legend Snippet: (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) Prox1- and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone

    Techniques Used: Labeling, Staining


    Structured Review

    Proteintech rabbit anti prox1
    Rabbit Anti Prox1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti prox1 - by Bioz Stars, 2024-07
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    Structured Review

    Proteintech rabbit anti prox1
    Rabbit Anti Prox1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti prox1/product/Proteintech
    Average 86 stars, based on 1 article reviews
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    rabbit anti prox1 - by Bioz Stars, 2024-07
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    Structured Review

    Proteintech prox1 antibody
    Prox1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prox1 antibody/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prox1 antibody - by Bioz Stars, 2024-07
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    Novus Biologicals mouse anti prox1
    A) Diffusion maps of rat intestinal epithelial cells, showing their differentiation as a measure of pseudotime. E1-E6 denote enterocytes of increasing maturation. B) Reclustered UMAP of cells arising from stem and secretory progenitor clusters. C) Diffusion maps of stem cells and secretory progenitors as a readout of pseudotime. Note the clear bifurcation between Goblet/Paneth and the EEC/Tuft/CHE lineages. D) Diffusion map color coded for Atoh1 expression. E) Atoh1 (magenta) and Goblet cell staining (Muc2; cyan). F) Atoh1 (magenta) and Best4 (cyan; CHE marker) costaining. G) Atoh1 (magenta) and Dclk1 (cyan; Tuft cell marker) costaining. H) Diffusion map of stem and secretory cells color coded for <t>Prox1</t> expression. I) CFTR (cyan; CHE marker) and Prox1 (magenta) co-staining. J) ChgA (cyan; EEC marker) and Prox1 (magenta) costaining. Arrow marks Prox1+/ChgA+ cell, red arrowhead marks Prox1-/ChgA+ cell. K-K’) Dclk1 (cyan, Tuft cell marker) and Prox1 costaining (in magenta, K’). L) Proportion of Tuft cells and EECs in rat proximal jejunum that are Prox1+. n=2 animals. Error bars, SD. M) Diffusion map of stem and secretory cells color-coded for Meis1 expression. N) Meis1 (magenta) and CFTR (cyan, CHE marker) costaining. DAPI in white. O-Q) Meis1 (magenta) staining with O) Muc2 (cyan, Goblet cells), P) Dclk1 (cyan, Tuft cells) or Q) ChgA (cyan, EECs). All scale bars, 10 μm.
    Mouse Anti Prox1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti prox1/product/Novus Biologicals
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    mouse anti prox1 - by Bioz Stars, 2024-07
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    R&D Systems prox1
    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, <t>Prox1,</t> and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prox1/product/R&D Systems
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    prox1 - by Bioz Stars, 2024-07
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    Thermo Fisher monoclonal mouse prox 1
    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, <t>Prox1,</t> and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Monoclonal Mouse Prox 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal mouse prox 1/product/Thermo Fisher
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    Reliatech anti prox1 antibody
    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, <t>Prox1,</t> and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Anti Prox1 Antibody, supplied by Reliatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti prox1 antibody/product/Reliatech
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    anti prox1 antibody - by Bioz Stars, 2024-07
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    R&D Systems anti prox1
    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, <t>Prox1,</t> and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Anti Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti prox1/product/R&D Systems
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    Millipore anti prox1
    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, <t>Prox1,</t> and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.
    Anti Prox1, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Danaher Inc rabbit anti prox1
    (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) <t>Prox1-</t> and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone
    Rabbit Anti Prox1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti prox1/product/Danaher Inc
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    Proteintech rabbit anti prox1
    (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) <t>Prox1-</t> and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone
    Rabbit Anti Prox1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti prox1 - by Bioz Stars, 2024-07
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    Proteintech prox1 antibody
    (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) <t>Prox1-</t> and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone
    Prox1 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    A) Diffusion maps of rat intestinal epithelial cells, showing their differentiation as a measure of pseudotime. E1-E6 denote enterocytes of increasing maturation. B) Reclustered UMAP of cells arising from stem and secretory progenitor clusters. C) Diffusion maps of stem cells and secretory progenitors as a readout of pseudotime. Note the clear bifurcation between Goblet/Paneth and the EEC/Tuft/CHE lineages. D) Diffusion map color coded for Atoh1 expression. E) Atoh1 (magenta) and Goblet cell staining (Muc2; cyan). F) Atoh1 (magenta) and Best4 (cyan; CHE marker) costaining. G) Atoh1 (magenta) and Dclk1 (cyan; Tuft cell marker) costaining. H) Diffusion map of stem and secretory cells color coded for Prox1 expression. I) CFTR (cyan; CHE marker) and Prox1 (magenta) co-staining. J) ChgA (cyan; EEC marker) and Prox1 (magenta) costaining. Arrow marks Prox1+/ChgA+ cell, red arrowhead marks Prox1-/ChgA+ cell. K-K’) Dclk1 (cyan, Tuft cell marker) and Prox1 costaining (in magenta, K’). L) Proportion of Tuft cells and EECs in rat proximal jejunum that are Prox1+. n=2 animals. Error bars, SD. M) Diffusion map of stem and secretory cells color-coded for Meis1 expression. N) Meis1 (magenta) and CFTR (cyan, CHE marker) costaining. DAPI in white. O-Q) Meis1 (magenta) staining with O) Muc2 (cyan, Goblet cells), P) Dclk1 (cyan, Tuft cells) or Q) ChgA (cyan, EECs). All scale bars, 10 μm.

    Journal: bioRxiv

    Article Title: A second wave of Notch signaling diversifies the intestinal secretory lineage

    doi: 10.1101/2024.07.15.603542

    Figure Lengend Snippet: A) Diffusion maps of rat intestinal epithelial cells, showing their differentiation as a measure of pseudotime. E1-E6 denote enterocytes of increasing maturation. B) Reclustered UMAP of cells arising from stem and secretory progenitor clusters. C) Diffusion maps of stem cells and secretory progenitors as a readout of pseudotime. Note the clear bifurcation between Goblet/Paneth and the EEC/Tuft/CHE lineages. D) Diffusion map color coded for Atoh1 expression. E) Atoh1 (magenta) and Goblet cell staining (Muc2; cyan). F) Atoh1 (magenta) and Best4 (cyan; CHE marker) costaining. G) Atoh1 (magenta) and Dclk1 (cyan; Tuft cell marker) costaining. H) Diffusion map of stem and secretory cells color coded for Prox1 expression. I) CFTR (cyan; CHE marker) and Prox1 (magenta) co-staining. J) ChgA (cyan; EEC marker) and Prox1 (magenta) costaining. Arrow marks Prox1+/ChgA+ cell, red arrowhead marks Prox1-/ChgA+ cell. K-K’) Dclk1 (cyan, Tuft cell marker) and Prox1 costaining (in magenta, K’). L) Proportion of Tuft cells and EECs in rat proximal jejunum that are Prox1+. n=2 animals. Error bars, SD. M) Diffusion map of stem and secretory cells color-coded for Meis1 expression. N) Meis1 (magenta) and CFTR (cyan, CHE marker) costaining. DAPI in white. O-Q) Meis1 (magenta) staining with O) Muc2 (cyan, Goblet cells), P) Dclk1 (cyan, Tuft cells) or Q) ChgA (cyan, EECs). All scale bars, 10 μm.

    Article Snippet: The remaining primary antibodies are commercially available: Mouse anti-Meis1 (1:100 O/N, Invitrogen, MA5-27191), Mouse anti-Prox1 (1:100 O/N, Novus Biologicals, NBP1-30045), Goat anti-Notch2 (1:100 15min, R&D Systems, AF1190-SP), Rabbit anti-Muc2 (1:1000 O/N, Abcam ab272692), Rabbit anti-Dclk1 (1:1000 O/N, Abcam ab31704), Rabbit anti-ChgA (requires prefixation, 1:500 O/N, Abcam, ab254557), Sheep anti-Dll1 (1:100 O/N, R&D Systems, AF5026-SP), Rabbit anti-Ki67 (1:200,1hr, Abcam, ab15580), Rabbit anti-Phospho Histone3 (1:500, 1hr, Cell Signaling, 3377), Rabbit anti-Hes1* (1:2500 Cell Signaling, 11988S) * requires 7.5 min incubation with Tyramide Signal Amplification Kit (ThermoFisher, B40922) Secondary antibodies were used at 1:200 as follows, except for DAPI (5µg/mL, Invitrogen, D1306).

    Techniques: Diffusion-based Assay, Expressing, Staining, Marker

    A) Dotplot from scRNA-seq data showing relative expression of factors associated with Notch signaling across intestinal epithelial cell types. B-C) Module scores for Notch-on (B) and Notch-off (C) gene signatures. D) Meis1 (magenta, CHEs) and Notch2 receptor (cyan) costaining. DAPI in white. E) Rat proximal jejunum stained for Best4 (cyan), Prox1 (yellow), and Notch2 (magenta). E’) triple positive CHEs in the differentiated villus region, E’’-E’’’) Notch2+/Prox1+ cells in crypts that do not express Best4. Color of insert box denotes approximate localization along the crypt-villus axis (left). DAPI in white. F) Rat proximal jejunum stained for Notch2 (magenta) and Meis1 (yellow) with CFTR (cyan). Color of insert box denotes approximate localization along the crypt-villus axis (left; F’-F”’). In F”’, magenta asterisk denotes a stromal non-epithelial expressing Meis1. G-G”) Notch2+ /Prox1+ cell expressing Ki67. Notch2 in yellow, Prox1 in magenta, Ki67 in cyan. H-H’) Notch2+/Prox1+ crypt cells in active mitosis. Note symmetric cell division for both Notch2 (yellow) and Prox1 (magenta). H’) Low level of phospho-histone 3 (in cyan) as cell is exiting mitosis. I-I’) Notch2+/Meis1 Low cells in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. J-J’) Notch2+ cell lacking Meis1 in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. All scale bars, 10 μm.

    Journal: bioRxiv

    Article Title: A second wave of Notch signaling diversifies the intestinal secretory lineage

    doi: 10.1101/2024.07.15.603542

    Figure Lengend Snippet: A) Dotplot from scRNA-seq data showing relative expression of factors associated with Notch signaling across intestinal epithelial cell types. B-C) Module scores for Notch-on (B) and Notch-off (C) gene signatures. D) Meis1 (magenta, CHEs) and Notch2 receptor (cyan) costaining. DAPI in white. E) Rat proximal jejunum stained for Best4 (cyan), Prox1 (yellow), and Notch2 (magenta). E’) triple positive CHEs in the differentiated villus region, E’’-E’’’) Notch2+/Prox1+ cells in crypts that do not express Best4. Color of insert box denotes approximate localization along the crypt-villus axis (left). DAPI in white. F) Rat proximal jejunum stained for Notch2 (magenta) and Meis1 (yellow) with CFTR (cyan). Color of insert box denotes approximate localization along the crypt-villus axis (left; F’-F”’). In F”’, magenta asterisk denotes a stromal non-epithelial expressing Meis1. G-G”) Notch2+ /Prox1+ cell expressing Ki67. Notch2 in yellow, Prox1 in magenta, Ki67 in cyan. H-H’) Notch2+/Prox1+ crypt cells in active mitosis. Note symmetric cell division for both Notch2 (yellow) and Prox1 (magenta). H’) Low level of phospho-histone 3 (in cyan) as cell is exiting mitosis. I-I’) Notch2+/Meis1 Low cells in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. J-J’) Notch2+ cell lacking Meis1 in active mitosis. Notch2 (magenta), Meis1 (yellow), DAPI in white. All scale bars, 10 μm.

    Article Snippet: The remaining primary antibodies are commercially available: Mouse anti-Meis1 (1:100 O/N, Invitrogen, MA5-27191), Mouse anti-Prox1 (1:100 O/N, Novus Biologicals, NBP1-30045), Goat anti-Notch2 (1:100 15min, R&D Systems, AF1190-SP), Rabbit anti-Muc2 (1:1000 O/N, Abcam ab272692), Rabbit anti-Dclk1 (1:1000 O/N, Abcam ab31704), Rabbit anti-ChgA (requires prefixation, 1:500 O/N, Abcam, ab254557), Sheep anti-Dll1 (1:100 O/N, R&D Systems, AF5026-SP), Rabbit anti-Ki67 (1:200,1hr, Abcam, ab15580), Rabbit anti-Phospho Histone3 (1:500, 1hr, Cell Signaling, 3377), Rabbit anti-Hes1* (1:2500 Cell Signaling, 11988S) * requires 7.5 min incubation with Tyramide Signal Amplification Kit (ThermoFisher, B40922) Secondary antibodies were used at 1:200 as follows, except for DAPI (5µg/mL, Invitrogen, D1306).

    Techniques: Expressing, Staining

    Working model of CHE cell differentiation in which: 1) CHEs arise from the secretory lineage, which is initially Notch off, 2) Notch is reactivated along the secretory lineage as a potentially multipotent Notch2+/Prox1+ progenitor and 3) Meis1 is upregulated to promote the expression of differentiated CHE markers, including CFTR and Best4.

    Journal: bioRxiv

    Article Title: A second wave of Notch signaling diversifies the intestinal secretory lineage

    doi: 10.1101/2024.07.15.603542

    Figure Lengend Snippet: Working model of CHE cell differentiation in which: 1) CHEs arise from the secretory lineage, which is initially Notch off, 2) Notch is reactivated along the secretory lineage as a potentially multipotent Notch2+/Prox1+ progenitor and 3) Meis1 is upregulated to promote the expression of differentiated CHE markers, including CFTR and Best4.

    Article Snippet: The remaining primary antibodies are commercially available: Mouse anti-Meis1 (1:100 O/N, Invitrogen, MA5-27191), Mouse anti-Prox1 (1:100 O/N, Novus Biologicals, NBP1-30045), Goat anti-Notch2 (1:100 15min, R&D Systems, AF1190-SP), Rabbit anti-Muc2 (1:1000 O/N, Abcam ab272692), Rabbit anti-Dclk1 (1:1000 O/N, Abcam ab31704), Rabbit anti-ChgA (requires prefixation, 1:500 O/N, Abcam, ab254557), Sheep anti-Dll1 (1:100 O/N, R&D Systems, AF5026-SP), Rabbit anti-Ki67 (1:200,1hr, Abcam, ab15580), Rabbit anti-Phospho Histone3 (1:500, 1hr, Cell Signaling, 3377), Rabbit anti-Hes1* (1:2500 Cell Signaling, 11988S) * requires 7.5 min incubation with Tyramide Signal Amplification Kit (ThermoFisher, B40922) Secondary antibodies were used at 1:200 as follows, except for DAPI (5µg/mL, Invitrogen, D1306).

    Techniques: Cell Differentiation, Expressing

    Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, Prox1, and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.

    Journal: bioRxiv

    Article Title: Ventricular lymphatic vessel density correlates with mild cardiac remodelling and preserved heart function in moderate murine aortic valve stenosis

    doi: 10.1101/2024.07.06.602334

    Figure Lengend Snippet: Histologic quantification of cardiac vessels: A ) Pan-endothelial marker: CD31, B ) lymphatic markers: Lyve1, Podocalyxin, Prox1, and C ) lymphatic mediator Reelin. Counterstaining of nuclei: 4’,6-diamidino-2-phenylindole (DAPI). Images were taken at 20x magnification. Cardiac area for the respective marker in relation to number of DAPI-stained nuclei was calculated. Displayed are mean values ± SD of n=5 (sham) and n=3 (wire injury/ WI) animals. For statistical analysis, Mann-Whitney test was used, * = p<0.05.

    Article Snippet: Primary antibodies: Vimentin (cell signaling, #5741), desmin (abcam, #ab15200), N-Cadherin (cell signaling, #13116), β-Catenin (cell signaling, #8480), TGF-β (Thermo Fisher, #PA1-29032), CD31 (R&D Systems, #AF3628), Lyve1 (R&D Systems, # AF2125), Podocalyxin (R&D Systems, #AF1556), Prox1 (R&D Systems, #AF2727) and Reelin (R&D Systems, #AF3820).

    Techniques: Marker, Staining, MANN-WHITNEY

    (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) Prox1- and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone

    Journal: bioRxiv

    Article Title: Single-Cell and Spatial Transcriptomic Analyses Reveals the Dynamic Transcript Profiles of Myocardial Lymphangiogenesis post Myocardial Infarction

    doi: 10.1101/2024.06.10.598235

    Figure Lengend Snippet: (A) Time points for heart harvest after the establishment of the MI models. (B) Transthoracic echocardiography confirmation of the MI mouse model. (C) Prox1- and Lyve1-labeled cardiac lymphatics in IF-stained frozen tissue section. (D) Lyve1-labeled collecting lymphatics with the lymphatic valves highlighted with white arrows. (E) IF-stained tissue section of the cardiac lymphatics in the remote zone

    Article Snippet: For Immunofluorescence(IF), frozen section were blocked with 5% normal goat serum (BioGenex, Fremont, CA) at room temperature for 1hour, and then stained with primary antibodies including, rabbit anti-Lyve1 (1:300, ab14917, Abcam, UK), rabbit anti-Prox1(1:300, ab199359, Abcam, UK) and mouse anti-Aqp1(1:300, sc-25287, Santa, USA) overnight at 4℃, Alexa Fluor 488- or 595-conjugated secondary antibodies including, wheat germ agglutinin (WGA) (1:500, Sigma-Aldrich) in the dark for 1 hours at room temperature on secondary morning.

    Techniques: Labeling, Staining