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Leinco Technologies anti proliferating cell nuclear antigen pcna
( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for <t>F4/80</t> in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) <t>PCNA</t> + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P
Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Leinco Technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti proliferating cell nuclear antigen pcna/product/Leinco Technologies
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti proliferating cell nuclear antigen pcna - by Bioz Stars, 2020-09
91/100 stars

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1) Product Images from "YB‐1 orchestrates onset and resolution of renal inflammation via IL10 gene regulation"

Article Title: YB‐1 orchestrates onset and resolution of renal inflammation via IL10 gene regulation

Journal: Journal of Cellular and Molecular Medicine

doi: 10.1111/jcmm.13260

( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for F4/80 in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) PCNA + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P
Figure Legend Snippet: ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for F4/80 in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) PCNA + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P

Techniques Used: Immunofluorescence, Marker, Mouse Assay, Quantitative RT-PCR, Immunostaining, Staining, Expressing

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Article Title: YB‐1 orchestrates onset and resolution of renal inflammation via IL10 gene regulation
Article Snippet: .. Renal tissues were stained using the following primary antibodies: anti‐human Col1A1 (Southern Biotech, Birmingham, AL), anti‐Kim1 (R & D systems, USA), anti‐mouse F4/80 (Serotec, Düsseldorf, Germany), anti‐proliferating cell nuclear antigen (PCNA) (Leinco Technologies, St. Louis, MO) and anti‐Ly6G (BD Biosciences, San Jose, CA). .. Negative controls for the immunohistochemical procedures consisted of substitution of the primary antibody with non‐immune IgG.

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    Leinco Technologies anti proliferating cell nuclear antigen pcna
    ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for <t>F4/80</t> in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) <t>PCNA</t> + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Leinco Technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti proliferating cell nuclear antigen pcna/product/Leinco Technologies
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti proliferating cell nuclear antigen pcna - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    85
    Leinco Technologies fitc coupled anti pcna
    ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for <t>F4/80</t> in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) <t>PCNA</t> + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P
    Fitc Coupled Anti Pcna, supplied by Leinco Technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc coupled anti pcna/product/Leinco Technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc coupled anti pcna - by Bioz Stars, 2020-09
    85/100 stars
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    ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for F4/80 in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) PCNA + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: YB‐1 orchestrates onset and resolution of renal inflammation via IL10 gene regulation

    doi: 10.1111/jcmm.13260

    Figure Lengend Snippet: ( A ) Immunofluorescence of tubular damage marker KIM ‐1 in WT and in Yb1 +/− mice. ( B ) Transcript numbers of chemokine CCL 5 were determined by qRT ‐ PCR in kidneys of WT and Yb1 +/− mice following I/R at different time‐points ( n = 5–10). ( C/D ) Numbers of renal infiltrating immune cells positive for Ly6G in renal medulla on day 1 and in renal cortex on days 1, 5 and 21 ( C ) and immune cells positive for F4/80 in cortex ( D ) in Yb1 +/− compared to WT mice following I/R ( n = 5–14). ( E/F ) Representative images of Col1 immunostaining ( E ) and quantification thereof by computer‐based morphometric analyses of the positively stained cortical area (%) ( n = 5–7) ( F ). In Yb1 +/− mice, cortical Col1A1 deposition was significantly less on day 5 but more on day 21 compared to their WT littermates. ( G/H ) PCNA + tubular nuclei in post‐ischaemic Yb1 +/− mice in comparison with WT mice on different days ( G ) and representative images on day 21 ( n = 5–11) ( H ). ( I–L ) Tubular damage in PAS ‐stained cortical tissue in I/R groups (I) and (J/K) quantification of tubular PCNA + nuclei ( J ) and F4/80 + cells ( K ) and NGAL mRNA expression ( L ) in kidneys following shortened ischaemia (25 min.) time ( n = 4–5). Scale bars, 50 μm. Data are expressed as mean values ± S.D. * P

    Article Snippet: Renal tissues were stained using the following primary antibodies: anti‐human Col1A1 (Southern Biotech, Birmingham, AL), anti‐Kim1 (R & D systems, USA), anti‐mouse F4/80 (Serotec, Düsseldorf, Germany), anti‐proliferating cell nuclear antigen (PCNA) (Leinco Technologies, St. Louis, MO) and anti‐Ly6G (BD Biosciences, San Jose, CA).

    Techniques: Immunofluorescence, Marker, Mouse Assay, Quantitative RT-PCR, Immunostaining, Staining, Expressing