Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti proliferating cell nuclear antigen pcna/product/Cell Signaling Technology Inc
Average 99 stars, based on 8 article reviews
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1) Product Images from "An Interaction of Renin-Angiotensin and Kallikrein-Kinin Systems Contributes to Vascular Hypertrophy in Angiotensin II-Induced Hypertension: In Vivo and In Vitro Studies"
Article Title: An Interaction of Renin-Angiotensin and Kallikrein-Kinin Systems Contributes to Vascular Hypertrophy in Angiotensin II-Induced Hypertension: In Vivo and In Vitro Studies
Journal: PLoS ONE
Figure Legend Snippet: Effects of des-Arg 9 -bradykinin (DABK) and angiotensin II (ANG II) vascular smooth muscle cells (VSMC) growth. [A] Aortic VSMC from Wistar rats treated with ANG II and/or DABK at low concentration (0.1 nM) in the presence or not of losartan (LOS – 10 µM), des-arg9-leu8-bradykinin (DAL – 10 µM) and TIRON (1 mM). Bar graph shows PCNA expression normalized against the housekeeping protein β-actin. Results are represented as mean±SEM of 4 experiments. *P
Techniques Used: Concentration Assay, Expressing
2) Product Images from "Inhibition of Intestinal Adenoma Formation in APCMin/+ Mice by Riccardin D, a Natural Product Derived from Liverwort Plant Dumortiera hirsuta"
Article Title: Inhibition of Intestinal Adenoma Formation in APCMin/+ Mice by Riccardin D, a Natural Product Derived from Liverwort Plant Dumortiera hirsuta
Journal: PLoS ONE
Figure Legend Snippet: Riccardin D decreased expression of β-catenin and cyclin D1 in intestinal polyps. Intestinal sections were processed for β-catenin and cyclin D1 immunohistochemistry staining. Intestinal sections of control and Riccardin D-treated mice showed brown-colored PCNA-positive (A) and cyclin D1-positive cells (B) in polyps on small intestines and colons (400×). Bar represents the mean ± S.D of six animals. *, p
Techniques Used: Expressing, Immunohistochemistry, Staining, Mouse Assay
3) Product Images from "MEF2D Deficiency in Neonatal Cardiomyocytes Triggers Cell Cycle Re-entry and Programmed Cell Death in Vitro *"
Article Title: MEF2D Deficiency in Neonatal Cardiomyocytes Triggers Cell Cycle Re-entry and Programmed Cell Death in Vitro *
Journal: The Journal of Biological Chemistry
Figure Legend Snippet: Microarray validation in NRVMs. A , summary of a subset of cell cycle genes dysregulated by 1.5-fold or more in MEF2D depleted NRVMs. The majority of the positive cell cycle genes are up-regulated. Cdt1 , chromatin licensing and DNA replication factor 1; Ccne1 , cyclin E1; Ccne2 , cyclin E2; Mcm3 , minichromosome maintenance complex component 3; Mcm5 , minichromosome maintenance complex component 5; Mcm5 , minichromosome maintenance complex component 6. B , quantitative RT-PCR analysis of cell cycle regulatory genes dysregulated in MEF2D-depleted NRVMs at day 3. Pcna , proliferating cell nuclear antigen. C , Western blot analysis of cell cycle-associated proteins cyclin D1, cyclin D3, and cyclin-dependent kinase 2 ( CDK2 ). D , Western blot densitometry. The data are means ± S.E. ( error bars ). *, p
Techniques Used: Microarray, Quantitative RT-PCR, Western Blot
Figure Legend Snippet: PI3K/Akt inhibition rescues apoptotic cell death associated with MEF2D depletion. A , summary of apoptosis-associated genes dysregulated by 1.5-fold or more in MEF2D-depleted NRVMs. B , quantitative RT-PCR analysis of the activating E2Fs, E2f1 , E2f2 , and E2f3 , revealed that MEF2D deficiency results in their increased expression. C , two pro-apoptotic transcriptional targets of E2F, Apaf1 and Casp8 , are significantly up-regulated in MEF2D depleted NRVMs. Up-regulation of E2f1 , E2f2 , and E2f3 transcript levels ( D ) and the E2F pro-apoptotic targets Apaf1 and Casp8 ( E ) is abolished when PI3K/Akt activation is inhibited by the addition of GDC-0941 in MEF2D-depleted NRVMs. F , TUNEL assay of NRVMs treated with GDC-0941 demonstrates an abrogation of apoptosis observed in MEF2D-deficient NRVMs 5 days post-transduction. G , post-natal cardiomyocytes are terminally differentiated and are unable to re-enter the cell cycle. MEF2D depletion results in an up-regulation of Mcm3 , Mcm5 , Mcm6 , Pcna , Ccne1 , and Ccne2 and aberrant cell cycle re-entry. E2F transcription factors likely sense unprogrammed cell cycle activation and mediate cardiomyocyte apoptosis by transcriptionally activating pro-apoptotic genes Apaf1 and Casp8. Apaf1 , apoptotic peptidase-activating factor 1; Casp8 , caspase-8. The data are means ± S.E. ( error bars ). *, p
Techniques Used: Inhibition, Quantitative RT-PCR, Expressing, Activation Assay, TUNEL Assay, Transduction
4) Product Images from "OCT4 maintains self-renewal and reverses senescence in human hair follicle mesenchymal stem cells through the downregulation of p21 by DNA methyltransferases"
Article Title: OCT4 maintains self-renewal and reverses senescence in human hair follicle mesenchymal stem cells through the downregulation of p21 by DNA methyltransferases
Journal: Stem Cell Research & Therapy
Figure Legend Snippet: DNMT inhibition decreased OCT4-increased proliferation capacity and differentiation potential in hHFMSCs. a The impact of 5-aza-dC (100 μM, 72 h) and zebularine (100 μM, 48 h) treatment on the expression of DNMTs, p21, OCT4, PCNA, and cyclin D1. b Immunofluorescence of proliferation-associated protein Ki67 (left panels) and OCT4 (right panels) expression and location after DNMT inhibition (bar, 50 μm). c MSP of p21 in hHFMSCs OCT4 treated with DMSO, 5-aza-dC, or zebularine. Cell proliferation curve ( d ) and clone formation assay ( e ) after DNMT inhibition; and the enlarged views showed the difference between DMSO and DNMT inhibitor-treated hHFMSCs OCT4 . The histogram is the formation rate of the three clones. f Effects of DNMT inhibition on hHFMSC cell cycle phase distribution. The percentage of the G1, G2, and S phases in the cell cycle (left of the lower panel) and the PI (right of the lower panel) after DNMT inhibition. “dip” is the abbreviation for diploid. g Osteogenic differentiation after DNMT inhibition. Calcium nodules were detected by Alizarin Red S staining (bar, 50 μm). (* p
Techniques Used: Inhibition, Expressing, Immunofluorescence, Tube Formation Assay, Clone Assay, Staining
Figure Legend Snippet: OCT4 increased proliferation capacity and differentiation potential in hHFMSCs. Cell proliferation curve ( a ) and cell population doubling time ( b ) of hHFMSCs EGFP and hHFMSCs OCT4 . c Clone formation assay of hHFMSCs EGFP and hHFMSCs OCT4 ; the enlarged views show the difference between the two cell clones. The histogram is the formation rate for each clone (bar, 200 μm). d Representative sphere images and bar graph of the percentages of spheres in hHFMSCs EGFP and hHFMSCs OCT . e Western blot results for the expression of proliferation-associated proteins PCNA and cyclin D1 in hHFMSCs EGFP and hHFMSCs OCT4 . f Immunofluorescence of proliferation-associated protein Ki67 expression and location in hHFMSCs EGFP and hHFMSCs OCT4 (bar, 50 μm). g Effects of OCT4 on hHFMSC cell cycle phase distribution. The percentage of the G1, G2, and S phase in the cell cycle (left of the lower panel) and the PI (right of the lower panel) of the hHFMSCs EGFP and hHFMSCs OCT4 . “dip” is the abbreviation for diploid. h Osteogenic differentiation in hHFMSCs EGFP and hHFMSCs OCT4 . Calcium nodules were detected by Alizarin Red S staining (bar, 200 μm). (* p
Techniques Used: Tube Formation Assay, Clone Assay, Western Blot, Expressing, Immunofluorescence, Staining
5) Product Images from "Herbal composition of Cinnamomum cassia, Pinus densiflora, Curcuma longa and Glycyrrhiza glabra prevents atherosclerosis by upregulating p27 (Kip1) expression"
Article Title: Herbal composition of Cinnamomum cassia, Pinus densiflora, Curcuma longa and Glycyrrhiza glabra prevents atherosclerosis by upregulating p27 (Kip1) expression
Journal: BMC Complementary and Alternative Medicine
Figure Legend Snippet: Effect of cinnamon extract on cell cycle progression and cell cycle-related proteins. VSMCs cultured in serum-starved medium were stimulated with 25 ng/mL PDGF-BB and the effect in the presence of Kiom-18 extract (5–50 μg/ml) on the DNA synthesis ( a ) and the histogram data ( b ) for each phase of the cell cycle is shown. The cell cycle progression data are representative of three independent experiments. Moreover, the effect of Kiom-18 extract on cell cycle regulatory proteins stimulated by PDGF-BB, including cyclin D1/E1, CDK2/4, Rb, and PCNA as negative regulatory molecules ( c ), was measured using SDS-PAGE followed by immunoblotting. Total β-actin was used for normalization. These results were analyzed using densitometry; the values represent the percentage of the control stimulated by PDGF-BB. All values are expressed as the mean ± SEM ( n = 3). Significant differences from the PDGF control (PDGF-stimulated) are shown by * p
Techniques Used: Cell Culture, DNA Synthesis, SDS Page
6) Product Images from "Macrophage-stimulated microRNA expression in mural cells promotes transplantation-induced neointima formation"
Article Title: Macrophage-stimulated microRNA expression in mural cells promotes transplantation-induced neointima formation
Figure Legend Snippet: Effects of macrophage depletion with clodronate liposomes on transplantation-induced arterial remodeling and inflammation ( A ) Time course of early adventitial macrophage infiltration and subsequent neointimal hyperplasia revealed by hematoxylin and eosin (H E) staining and immunohistochemistry. Macrophages were stained with anti-CD68; T cells stained with anti-CD3; and proliferating cells stained with anti-PCNA. ( B ) Histology images showing that macrophage depletion inhibited the growth of neointima, diminished infiltrating macrophages in the adventitia, reduced the number of PCNA + cells, and reduced inflammatory responses (samples of day 14). ( C ) Effects of clodronate on the expression levels of various inflammatory molecules measured with qPCR. ( D ) Proportion of smooth muscle cells (stained with anti-a-SM actin, indicated by arrows) and non-muscle cells (arrowheads) in the neointima of untreated and clodronate-treated vessels. Data are mean ± SD. * P
Techniques Used: Transplantation Assay, Staining, Immunohistochemistry, Expressing, Real-time Polymerase Chain Reaction
7) Product Images from "Epigenetic reactivation of estrogen receptor-? (ER?) by genistein enhances hormonal therapy sensitivity in ER?-negative breast cancer"
Article Title: Epigenetic reactivation of estrogen receptor-? (ER?) by genistein enhances hormonal therapy sensitivity in ER?-negative breast cancer
Journal: Molecular Cancer
Figure Legend Snippet: GE and TAM inhibited the expression of PCNA and increased ERα expression in vivo . Immunohistochemical analysis was performed in tumor samples to detect PCNA-positive cells for proliferation index (left panel) and ERα in vivo expression (right panel). A ) and B ) PCNA and ERα expression in MDA-MB-231 tumor xenogratfs ( Protocol 1). C ) and D ) PCNA and ERα expression in C3(1)-SV40 Tag transgenic mice tumors ( Protocol 2). Immunohistochemical data in terms of percentage of positive cells are presented as mean ± SD from each group. PCNA-positive and ERα-positive cells were counted in 5 different areas of the sections, and data are summarized in terms of percent positive cells from all tumor samples. Representative photograph from one field of each experimental group. Columns, mean; Bars, SD from 5 or 10 mice per group; *, p
Techniques Used: Expressing, In Vivo, Immunohistochemistry, Multiple Displacement Amplification, Transgenic Assay, Mouse Assay
Article Title: Targeted Profiling of Heat Shock Proteome in Radioresistant Breast Cancer Cells
Article Snippet: ..
Article Title: Focused ultrasound delivery of a selective TrkA agonist rescues cholinergic function in a mouse model of Alzheimer’s disease
Article Snippet: .. The following primary antibodies were incubated overnight at 4°C: TrkA (1:1000; Cell Signaling, 2505), p75NTR (1:1000; Santa Cruz Biotechnology, sc-271708), pAkt (1:1000; Cell Signaling, 4060), Akt (1:1000; Cell Signaling, 9272), pCREB (1:1500; Cell Signaling, 9198), CREB (1:1500; Cell Signaling, 9104),
Article Title: Maternal L-Carnitine Supplementation Improves Brain Health in Offspring from Cigarette Smoke Exposed Mothers
Article Snippet: .. Protein samples (20 μg) were separated on NuPage® Novex® 4%–12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes; 1:2500, Abcam, Cambridge, UK), Drp-1 (1:2000, Novus Biotechnology, Littleton, CO, USA), Opa-1 (1:2000, Novus Biotechnology, Littleton, CO, USA),
Article Title: Chlorogenic Acid (CGA) Isomers Alleviate Interleukin 8 (IL-8) Production in Caco-2 Cells by Decreasing Phosphorylation of p38 and Increasing Cell Integrity
Article Snippet: .. Specifically, the membrane that was used for the detection of p-Erk1/2 was washed three times with TBST and incubated in a mixture antibody solution that contained both
Article Title: Total flavonoids extracted from Nervilia Fordii function in polycystic ovary syndrome through IL-6 mediated JAK2/STAT3 signaling pathway
Article Snippet: .. Incubated with
Article Title: Fibroblast Growth Factor 2 Regulates High Mobility Group A2 Expression in Human Bone Marrow-Derived Mesenchymal Stem Cells
Article Snippet: .. Proteins were transferred onto PVDF membranes and incubated overnight with primary