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Becton Dickinson anti proliferating cell nuclear antigen pcna
Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti proliferating cell nuclear antigen pcna/product/Becton Dickinson
Average 91 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti proliferating cell nuclear antigen pcna - by Bioz Stars, 2020-07
91/100 stars

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Article Title: Caffeic Acid Derivatives Inhibit the Growth of Colon Cancer: Involvement of the PI3-K/Akt and AMPK Signaling Pathways
Article Snippet: PI (propidium Iodine) and anti- proliferating cell nuclear antigen (PCNA) (#610664) monoclonal antibodies were purchased from BD Biosciences Inc. (Franklin Lakes, NJ).

Article Title: Oregano Essential Oil Attenuates RAW264.7 Cells from Lipopolysaccharide-Induced Inflammatory Response through Regulating NADPH Oxidase Activation-Driven Oxidative Stress
Article Snippet: The anti-proliferating cell nuclear antigen (PCNA) was from BD Transduction Laboratories (San Diego, CA, USA).

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  • 93
    Becton Dickinson anti pcna
    Arg 72 -p53 interacts directly with mitochondrial Bcl-xL and increases neuronal vulnerability to ischemia-induced apoptosis. (A) HEK293T cells were transfected with Arg 72 -p53 or Pro 72 -p53. Cytoplasmic (cyto) and mitochondrial (mito) fractions were isolated and analyzed by Western blotting. <t>VDAC,</t> control for the integrity of the mitochondria. <t>PCNA,</t> control indicating absence of nuclear contamination in the mitochondrial fractions. Black lines indicate that intervening lanes have been spliced out. (B) Rat cortical primary neurons were transfected with Arg 72 -p53-IRES-EGFP or Pro 72 -p53-IRES-EGFP. p53 colocalization with Bcl-xL was analyzed by immunofluorescence. DAPI, nuclear staining. (C) Rat cortical primary neurons were transfected with Arg 72 -p53-IRES-EGFP, Pro 72 -p53-IRES-EGFP, or Arg 72 -p53-IRES-EGFP + Bcl-xL. Apoptosis was measured 14 h later. (D) Neurons were transfected with empty vector (control) or the minimum amount of Arg 72 -p53-IRES-EGFP or Arg 72 -p53-IRES-EGFP cDNA not altering neuronal survival (0.08 µg/10 6 neurons) and were exposed to 100 µM glutamate for 5 min and incubated in culture medium for a further 8 h. Apoptosis was measured. (E) Neurons were transfected as described in D and were exposed to oxygen and glucose deprivation (OGD) for 1 h. Apoptosis was measured. (F) Neurons from Tp53 −/− mice were transfected with human BAC containing the entire Tp53 gene locus encoding either proline (p53 −/− Pro 72 ) or arginine (p53 −/− Arg 72 ) and were exposed to oxygen and glucose deprivation (OGD) for 3 h. Apoptosis was measured. The data in A and B represent four independent experiments. The data in C–F are means ± SEM of four different cell cultures. *, P
    Anti Pcna, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pcna/product/Becton Dickinson
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    anti pcna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    85
    Becton Dickinson mouse monoclonal anti human pcna antibody
    Newborn hypothalamic cells take on <t>AgRP</t> neuronal identity. (A-C) ). (D-G) X-gal staining combined with immunohistochemical analysis of <t>PCNA</t> or Ki-67 in hypothalamic sections from the AgRP-Tfam mutant animals. Control and mutant animals carried the Tg.AgRP-Cre ). In this preparation, LacZ expression is characteristically seen as perinuclear blue dots while brown nuclear staining represents PCNA or Ki-67 signal as indicated. Arrows show a LacZ/PCNA co-expressing neuron and a LacZ/Ki-67 co-expressing neuron. Panel E is a magnified view of the boxed area in Panel D, while Panel G is a magnified view of the boxed area in Panel F. The specificity of the Tg.AgRP-Cre ). 3V: third ventricle.
    Mouse Monoclonal Anti Human Pcna Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal anti human pcna antibody/product/Becton Dickinson
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal anti human pcna antibody - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Becton Dickinson immunohistochemical staining mouse monoclonal anti proliferating cell nuclear antigen pcna antibody
    <t>Immunohistochemical</t> detection of makers in endometriotic lesions. (a) Representative immunohistochemical images of ectopic endometrium from vehicle- and Shaofu Zhuyu decoction- (SZD-) treated rats are shown. Sections were stained with <t>PCNA</t> to detect proliferating cells, TUNEL assay to confirmed apoptosis, CD-34 to detected microvessels, and HIF-1 α to evaluate hypoxia status; magnification: ×100. (b) PCNA-positive (‰), apoptosis index (%), microvessel density (mm −2 ), and HIF-1 α -positive (%) cells in endometriotic lesions after treatment, analyzed with quantitative analysis of immunohistochemical sections. Mean ± standard deviation (SD). SZD group ( n = 8) versus vehicle group ( n = 7): ∗ P
    Immunohistochemical Staining Mouse Monoclonal Anti Proliferating Cell Nuclear Antigen Pcna Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical staining mouse monoclonal anti proliferating cell nuclear antigen pcna antibody/product/Becton Dickinson
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunohistochemical staining mouse monoclonal anti proliferating cell nuclear antigen pcna antibody - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Arg 72 -p53 interacts directly with mitochondrial Bcl-xL and increases neuronal vulnerability to ischemia-induced apoptosis. (A) HEK293T cells were transfected with Arg 72 -p53 or Pro 72 -p53. Cytoplasmic (cyto) and mitochondrial (mito) fractions were isolated and analyzed by Western blotting. VDAC, control for the integrity of the mitochondria. PCNA, control indicating absence of nuclear contamination in the mitochondrial fractions. Black lines indicate that intervening lanes have been spliced out. (B) Rat cortical primary neurons were transfected with Arg 72 -p53-IRES-EGFP or Pro 72 -p53-IRES-EGFP. p53 colocalization with Bcl-xL was analyzed by immunofluorescence. DAPI, nuclear staining. (C) Rat cortical primary neurons were transfected with Arg 72 -p53-IRES-EGFP, Pro 72 -p53-IRES-EGFP, or Arg 72 -p53-IRES-EGFP + Bcl-xL. Apoptosis was measured 14 h later. (D) Neurons were transfected with empty vector (control) or the minimum amount of Arg 72 -p53-IRES-EGFP or Arg 72 -p53-IRES-EGFP cDNA not altering neuronal survival (0.08 µg/10 6 neurons) and were exposed to 100 µM glutamate for 5 min and incubated in culture medium for a further 8 h. Apoptosis was measured. (E) Neurons were transfected as described in D and were exposed to oxygen and glucose deprivation (OGD) for 1 h. Apoptosis was measured. (F) Neurons from Tp53 −/− mice were transfected with human BAC containing the entire Tp53 gene locus encoding either proline (p53 −/− Pro 72 ) or arginine (p53 −/− Arg 72 ) and were exposed to oxygen and glucose deprivation (OGD) for 3 h. Apoptosis was measured. The data in A and B represent four independent experiments. The data in C–F are means ± SEM of four different cell cultures. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: The human Tp53 Arg72Pro polymorphism explains different functional prognosis in stroke

    doi: 10.1084/jem.20101523

    Figure Lengend Snippet: Arg 72 -p53 interacts directly with mitochondrial Bcl-xL and increases neuronal vulnerability to ischemia-induced apoptosis. (A) HEK293T cells were transfected with Arg 72 -p53 or Pro 72 -p53. Cytoplasmic (cyto) and mitochondrial (mito) fractions were isolated and analyzed by Western blotting. VDAC, control for the integrity of the mitochondria. PCNA, control indicating absence of nuclear contamination in the mitochondrial fractions. Black lines indicate that intervening lanes have been spliced out. (B) Rat cortical primary neurons were transfected with Arg 72 -p53-IRES-EGFP or Pro 72 -p53-IRES-EGFP. p53 colocalization with Bcl-xL was analyzed by immunofluorescence. DAPI, nuclear staining. (C) Rat cortical primary neurons were transfected with Arg 72 -p53-IRES-EGFP, Pro 72 -p53-IRES-EGFP, or Arg 72 -p53-IRES-EGFP + Bcl-xL. Apoptosis was measured 14 h later. (D) Neurons were transfected with empty vector (control) or the minimum amount of Arg 72 -p53-IRES-EGFP or Arg 72 -p53-IRES-EGFP cDNA not altering neuronal survival (0.08 µg/10 6 neurons) and were exposed to 100 µM glutamate for 5 min and incubated in culture medium for a further 8 h. Apoptosis was measured. (E) Neurons were transfected as described in D and were exposed to oxygen and glucose deprivation (OGD) for 1 h. Apoptosis was measured. (F) Neurons from Tp53 −/− mice were transfected with human BAC containing the entire Tp53 gene locus encoding either proline (p53 −/− Pro 72 ) or arginine (p53 −/− Arg 72 ) and were exposed to oxygen and glucose deprivation (OGD) for 3 h. Apoptosis was measured. The data in A and B represent four independent experiments. The data in C–F are means ± SEM of four different cell cultures. *, P

    Article Snippet: Aliquots of cell, mitochondrial, or cytosolic extracts were subjected to SDS polyacrylamide gel (Mini-PROTEAN; Bio-Rad Laboratories) and blotted with anti-p53, anti-p21, anticleaved PARP-1 (BD), anti-Bax, anti–active caspase 3 (Santa Cruz Biotechnology, Inc.), anti-PUMA, anti-PERP, anti-AIP1 (Abcam), anti–cytochrome c (Santa Cruz Biotechnology, Inc.), anti-VDAC (EMD), anti-PCNA (BD), or anti-GAPDH (Sigma-Aldrich) overnight at 4°C.

    Techniques: Transfection, Isolation, Western Blot, Immunofluorescence, Staining, Plasmid Preparation, Incubation, Mouse Assay, BAC Assay

    Actions of a NOX4 blocker in KGN. KGN treatment with GKT137831 resulted in a significant reduction in cell number ( A ; n = 6) and confluence ( B ; n = 4), as well as reduced PCNA content. The figure was cropped and the original gel images are shown in the supplementary data ( C ) PCNA in KGN compared to control (blotted membrane of one representative example is shown). Diagram shows PCNA levels of control versus treated cells normalized to β-actin (n = 4). ( D ) Treatment with GKT137831 had no cytotoxic effect in KGN compared to control (LDH cytotoxicity assay; n = 5). One-sample t -tests (two-tailed; *p

    Journal: Scientific Reports

    Article Title: The NADPH oxidase 4 is a major source of hydrogen peroxide in human granulosa-lutein and granulosa tumor cells

    doi: 10.1038/s41598-019-40329-8

    Figure Lengend Snippet: Actions of a NOX4 blocker in KGN. KGN treatment with GKT137831 resulted in a significant reduction in cell number ( A ; n = 6) and confluence ( B ; n = 4), as well as reduced PCNA content. The figure was cropped and the original gel images are shown in the supplementary data ( C ) PCNA in KGN compared to control (blotted membrane of one representative example is shown). Diagram shows PCNA levels of control versus treated cells normalized to β-actin (n = 4). ( D ) Treatment with GKT137831 had no cytotoxic effect in KGN compared to control (LDH cytotoxicity assay; n = 5). One-sample t -tests (two-tailed; *p

    Article Snippet: Protein samples were analyzed (7,5 µg of GCs on culture day 2 and 10 µg of KGN) with anti-NOX4 rabbit polyclonal antibody (Cat#: 7927, ProSci; Fort Collins, CO, USA), anti-PCNA (Cat#: 610664; BD), anti-MAPK (Cat#: 4695, Cell Signaling), anti-pMAPK (Cat#: 9106, Cell Signaling), as well as anti-β-Actin mouse monoclonal antibody (Cat#: A5441, Sigma-Aldrich).

    Techniques: LDH Cytotoxicity Assay, Two Tailed Test

    Newborn hypothalamic cells take on AgRP neuronal identity. (A-C) ). (D-G) X-gal staining combined with immunohistochemical analysis of PCNA or Ki-67 in hypothalamic sections from the AgRP-Tfam mutant animals. Control and mutant animals carried the Tg.AgRP-Cre ). In this preparation, LacZ expression is characteristically seen as perinuclear blue dots while brown nuclear staining represents PCNA or Ki-67 signal as indicated. Arrows show a LacZ/PCNA co-expressing neuron and a LacZ/Ki-67 co-expressing neuron. Panel E is a magnified view of the boxed area in Panel D, while Panel G is a magnified view of the boxed area in Panel F. The specificity of the Tg.AgRP-Cre ). 3V: third ventricle.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: De novo neurogenesis in adult hypothalamus as a compensatory mechanism to regulate energy balance

    doi: 10.1523/JNEUROSCI.2479-09.2010

    Figure Lengend Snippet: Newborn hypothalamic cells take on AgRP neuronal identity. (A-C) ). (D-G) X-gal staining combined with immunohistochemical analysis of PCNA or Ki-67 in hypothalamic sections from the AgRP-Tfam mutant animals. Control and mutant animals carried the Tg.AgRP-Cre ). In this preparation, LacZ expression is characteristically seen as perinuclear blue dots while brown nuclear staining represents PCNA or Ki-67 signal as indicated. Arrows show a LacZ/PCNA co-expressing neuron and a LacZ/Ki-67 co-expressing neuron. Panel E is a magnified view of the boxed area in Panel D, while Panel G is a magnified view of the boxed area in Panel F. The specificity of the Tg.AgRP-Cre ). 3V: third ventricle.

    Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal AGRP antibody ( ; ) and a mouse monoclonal anti-human PCNA antibody described above (1:4000, BD Pharmigen).

    Techniques: Staining, Immunohistochemistry, Mutagenesis, Expressing

    Newborn hypothalamic cells take on Pomc neuronal identity. (A) A representative field of the arcuate nucleus showing expression of PCNA (nuclear signal) and ACTH (product of the Pomc neurons, cytoplasmic signal) in an AgRP-Tfam mutant animal. DAPI marked all nuclei in the field. (B-D) ). 3V: third ventricle.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: De novo neurogenesis in adult hypothalamus as a compensatory mechanism to regulate energy balance

    doi: 10.1523/JNEUROSCI.2479-09.2010

    Figure Lengend Snippet: Newborn hypothalamic cells take on Pomc neuronal identity. (A) A representative field of the arcuate nucleus showing expression of PCNA (nuclear signal) and ACTH (product of the Pomc neurons, cytoplasmic signal) in an AgRP-Tfam mutant animal. DAPI marked all nuclei in the field. (B-D) ). 3V: third ventricle.

    Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal AGRP antibody ( ; ) and a mouse monoclonal anti-human PCNA antibody described above (1:4000, BD Pharmigen).

    Techniques: Expressing, Mutagenesis

    Newly generated hypothalamic cells are responsive to leptin. Four and a half month old AgRP-Tfam mutant mice were treated with either vehicle or leptin (5mg/kg) ip, and mice were killed 45 minutes later. Immunofluorescence analysis was carried out to identify cells that express pSTAT3 and PCNA. (A, B) Leptin but not saline treatment activated pSTAT3 in the arcuate nucleus of the hypothalamus. (C) A field in the arcuate nucleus of the hypothalamus showing expression of PCNA and leptin-induced pSTAT3 expression. Arrow indicates a colocalized cell. (D-F) Magnified views of the colocalized cell indicated by arrow in (C), showing PCNA, pSTAT3 immunoreactivity and merged images. Arrow indicates a colocalized cell. Twenty-four of 106 PCNA cells (22.6%) were found to be positive for pSTAT3. Fourteen sections from 6 mice were analyzed. 3V: third ventricle.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: De novo neurogenesis in adult hypothalamus as a compensatory mechanism to regulate energy balance

    doi: 10.1523/JNEUROSCI.2479-09.2010

    Figure Lengend Snippet: Newly generated hypothalamic cells are responsive to leptin. Four and a half month old AgRP-Tfam mutant mice were treated with either vehicle or leptin (5mg/kg) ip, and mice were killed 45 minutes later. Immunofluorescence analysis was carried out to identify cells that express pSTAT3 and PCNA. (A, B) Leptin but not saline treatment activated pSTAT3 in the arcuate nucleus of the hypothalamus. (C) A field in the arcuate nucleus of the hypothalamus showing expression of PCNA and leptin-induced pSTAT3 expression. Arrow indicates a colocalized cell. (D-F) Magnified views of the colocalized cell indicated by arrow in (C), showing PCNA, pSTAT3 immunoreactivity and merged images. Arrow indicates a colocalized cell. Twenty-four of 106 PCNA cells (22.6%) were found to be positive for pSTAT3. Fourteen sections from 6 mice were analyzed. 3V: third ventricle.

    Article Snippet: Following blocking, sections were incubated with a rabbit polyclonal AGRP antibody ( ; ) and a mouse monoclonal anti-human PCNA antibody described above (1:4000, BD Pharmigen).

    Techniques: Generated, Mutagenesis, Mouse Assay, Immunofluorescence, Expressing

    Immunohistochemical detection of makers in endometriotic lesions. (a) Representative immunohistochemical images of ectopic endometrium from vehicle- and Shaofu Zhuyu decoction- (SZD-) treated rats are shown. Sections were stained with PCNA to detect proliferating cells, TUNEL assay to confirmed apoptosis, CD-34 to detected microvessels, and HIF-1 α to evaluate hypoxia status; magnification: ×100. (b) PCNA-positive (‰), apoptosis index (%), microvessel density (mm −2 ), and HIF-1 α -positive (%) cells in endometriotic lesions after treatment, analyzed with quantitative analysis of immunohistochemical sections. Mean ± standard deviation (SD). SZD group ( n = 8) versus vehicle group ( n = 7): ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Shaofu Zhuyu Decoction Regresses Endometriotic Lesions in a Rat Model

    doi: 10.1155/2018/3927096

    Figure Lengend Snippet: Immunohistochemical detection of makers in endometriotic lesions. (a) Representative immunohistochemical images of ectopic endometrium from vehicle- and Shaofu Zhuyu decoction- (SZD-) treated rats are shown. Sections were stained with PCNA to detect proliferating cells, TUNEL assay to confirmed apoptosis, CD-34 to detected microvessels, and HIF-1 α to evaluate hypoxia status; magnification: ×100. (b) PCNA-positive (‰), apoptosis index (%), microvessel density (mm −2 ), and HIF-1 α -positive (%) cells in endometriotic lesions after treatment, analyzed with quantitative analysis of immunohistochemical sections. Mean ± standard deviation (SD). SZD group ( n = 8) versus vehicle group ( n = 7): ∗ P

    Article Snippet: Immunohistochemical Staining Mouse monoclonal anti-proliferating cell nuclear antigen (PCNA) antibody (1:50, 610664, BD, USA) was used to detect the proliferating cells.

    Techniques: Immunohistochemistry, Staining, TUNEL Assay, Standard Deviation