Structured Review

Agilent technologies anti proliferating cell nuclear antigen pcna
Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of <t>PCNA</t> , cleaved caspase‐3 and <t>GAPDH</t> in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P
Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti proliferating cell nuclear antigen pcna/product/Agilent technologies
Average 91 stars, based on 8 article reviews
Price from $9.99 to $1999.99
anti proliferating cell nuclear antigen pcna - by Bioz Stars, 2020-07
91/100 stars

Images

1) Product Images from "Pericardial patch venoplasty heals via attraction of venous progenitor cells. Pericardial patch venoplasty heals via attraction of venous progenitor cells"

Article Title: Pericardial patch venoplasty heals via attraction of venous progenitor cells. Pericardial patch venoplasty heals via attraction of venous progenitor cells

Journal: Physiological Reports

doi: 10.14814/phy2.12841

Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of PCNA , cleaved caspase‐3 and GAPDH in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P
Figure Legend Snippet: Cell turnover after patch venoplasty. (A) Representative Western blot showing expression of PCNA , cleaved caspase‐3 and GAPDH in the control IVC (vein), preimplantation patch (day 0), and patch explanted at day 7 or day 30; n = 3. (B) Immunohistochemical analysis of the neointima at day 0 (left column), day 7 (middle column), or day 30 (right column). Analysis for: upper row, proliferating cell nuclear antigen ( PCNA ) (scale bar, 100 μ m); lower row, cleaved caspase‐3 (scale bar, 50 μ m). P, patch; L, lumen; N, neointima. Yellow arrow shows the positive cells. n = 4. (C) Bar graph showing neointimal proliferation index (mean number of cells counted in 4 high power fields); P

Techniques Used: Western Blot, Expressing, Immunohistochemistry

2) Product Images from "Modulation of Fgfr1a Signaling in Zebrafish Reveals a Genetic Basis for the Aggression–Boldness Syndrome"

Article Title: Modulation of Fgfr1a Signaling in Zebrafish Reveals a Genetic Basis for the Aggression–Boldness Syndrome

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.2892-11.2011

spd have a reduction of Fgf signaling in the periventricular nucleus. a–j , High-magnification images of coronal sections through the adult zebrafish hypothalamus, showing dusp6 in situ hybridization ( a , b ), pERK immunocytochemistry ( c , d ), PCNA immunocytochemistry ( e , f ), TUNEL labeling ( g , h ), and Nissl staining ( i , j ). dusp6 and pERK are reduced in the PVN of spd ( b , d ) compared with siblings ( a , c ). PCNA and TUNEL labeling is similar between wild-type ( e , g ) and spd ( f , h ). The Nissl-stained PVN of spd ( j ) appears larger than wild-type ( i ). Arrowheads in a–d highlight the PVN. Arrowheads in e and f point to PCNA-positive cells. k , Diagram depicting the position of all sections in the zebrafish brain. l–o , The expression of mao ( l , m ) and slc6a4b ( n , o ) in the periventricular nucleus of both wild-type ( l , n ) and spd ( m , o ). Expression levels are similar in both genotypes. p–s , The expression of pomcb ( p , q ) and nvpfl ( r , s ) in the preoptic area of both wild-type ( p , r ) and spd ( q , s ). Expression levels are similar in both genotypes. PM, magnocellular preoptic nucleus; PPp, parvocellular preoptic nucleus posterior part; SC, suprachiasmatic nucleus.
Figure Legend Snippet: spd have a reduction of Fgf signaling in the periventricular nucleus. a–j , High-magnification images of coronal sections through the adult zebrafish hypothalamus, showing dusp6 in situ hybridization ( a , b ), pERK immunocytochemistry ( c , d ), PCNA immunocytochemistry ( e , f ), TUNEL labeling ( g , h ), and Nissl staining ( i , j ). dusp6 and pERK are reduced in the PVN of spd ( b , d ) compared with siblings ( a , c ). PCNA and TUNEL labeling is similar between wild-type ( e , g ) and spd ( f , h ). The Nissl-stained PVN of spd ( j ) appears larger than wild-type ( i ). Arrowheads in a–d highlight the PVN. Arrowheads in e and f point to PCNA-positive cells. k , Diagram depicting the position of all sections in the zebrafish brain. l–o , The expression of mao ( l , m ) and slc6a4b ( n , o ) in the periventricular nucleus of both wild-type ( l , n ) and spd ( m , o ). Expression levels are similar in both genotypes. p–s , The expression of pomcb ( p , q ) and nvpfl ( r , s ) in the preoptic area of both wild-type ( p , r ) and spd ( q , s ). Expression levels are similar in both genotypes. PM, magnocellular preoptic nucleus; PPp, parvocellular preoptic nucleus posterior part; SC, suprachiasmatic nucleus.

Techniques Used: In Situ Hybridization, Immunocytochemistry, TUNEL Assay, Labeling, Staining, Expressing

3) Product Images from "A hypoxia-dependent upregulation of hypoxia-inducible factor-1 by nuclear factor-κB promotes gastric tumour growth and angiogenesis"

Article Title: A hypoxia-dependent upregulation of hypoxia-inducible factor-1 by nuclear factor-κB promotes gastric tumour growth and angiogenesis

Journal: British Journal of Cancer

doi: 10.1038/sj.bjc.6606020

Representative immunohistochemical findings of the effect of I κ B α M overexpression in differently sized xenograft tumours derived from SNU-668 Vector cells (left lanes) or SNU-668 I κ B α M cells (right lanes). Tumour sections were stained with H E ( A and B ), anti-NF- κ B p65 ( C and D ), anti-PCNA ( E and F ), anti-cleaved caspase-3 ( G and H ), anti-CD31 ( I and J ), or anti-HIF-1 α ( K and L ). All sections were lightly counterstained with haematoxylin. Scale bars=50 μ m.
Figure Legend Snippet: Representative immunohistochemical findings of the effect of I κ B α M overexpression in differently sized xenograft tumours derived from SNU-668 Vector cells (left lanes) or SNU-668 I κ B α M cells (right lanes). Tumour sections were stained with H E ( A and B ), anti-NF- κ B p65 ( C and D ), anti-PCNA ( E and F ), anti-cleaved caspase-3 ( G and H ), anti-CD31 ( I and J ), or anti-HIF-1 α ( K and L ). All sections were lightly counterstained with haematoxylin. Scale bars=50 μ m.

Techniques Used: Immunohistochemistry, Over Expression, Derivative Assay, Plasmid Preparation, Staining

Related Articles

Immunohistochemistry:

Article Title: Spontaneous extraskeletal osteosarcoma with various histological growth patterns in the abdominal wall of an ICR mouse
Article Snippet: .. The primary antibodies used for immunohistochemistry were as follows: mouse anti-cytokeratin (AE1/AE3, monoclonal, prediluted, Dako, Glostrup, Denmark), anti-vimentin (V9, monoclonal, 1:50, Dako), anti-smooth muscle actin (SMA) (1A4, monoclonal, 1:100, Dako) and anti-proliferating cell nuclear antigen (PCNA) (PC10, monoclonal, 1:200, Dako), and rabbit anti-osterix (polyclonal, 1:200, Abcam, Cambridge, UK), anti-desmin (polyclonal, prediluted, Dako), anti-S100 (polyclonal, 1:400, Dako), anti-glial fibrillary acidic protein (GFAP) (polyclonal, prediluted, Dako), anti-factor VIII-related antigen (polyclonal, prediluted, BioGenex Laboratories, Fremont, CA, USA) and anti-ionized calcium binding adaptor molecule 1 (Iba1) (polyclonal, 1:500, Wako Pure Chemical Industries, Osaka, Japan). ..

Article Title: Pericardial patch venoplasty heals via attraction of venous progenitor cells. Pericardial patch venoplasty heals via attraction of venous progenitor cells
Article Snippet: .. Primary and secondary antibodies Primary antibodies included: anti‐α ‐actin (Abcam, ab5694; IHC and IF, 1:100; WB, 1:1000); anti‐cleaved Caspase‐3 (Cell Signaling #9661; IHC, 1:50; WB, 1:1000); anti‐CD31 (Abcam, ab28364; IHC and IF, 1:50); anti‐CD34 (R & D, AF4117; IF, 1:100; WB, 1:1000); anti‐CD45 (Abcam, ab10558; IHC, 1:50; WB, 1:1000); anti‐CD68 (ED1; Abcam, ab31630; IHC, 1:200; WB, 1:1000); anti‐ COUP‐TFII (Novus biologicals, NBP1‐67885; IHC, 1:100); anti‐Eph‐B4 (Santa Cruz, sc‐5536; IF, 1:50); anti‐dll‐4 (santa cruz, sc‐18640, 1:50); anti‐Eph‐B4 (Abcam, ab76657; IHC and IF, 1:50); anti‐Ephrin‐B2 (Novus, NBP1‐48610; IHC, 1:50); anti‐Eph‐B4 (R & D, AF446; WB, 1:1000); anti‐GAPDH (Cell Signaling, 14C10; WB, 1:2000); anti‐Ki67 (Abcam, ab15580; IHC, 1:100); anti‐proliferating cell nuclear antigen (PCNA) (Dako monoclonal mouse Anti‐PCNA; IHC and IF, 1:100; WB, 1:1000); anti‐VEGFR2 (ABCAM, ab2349; IF, 1:100; WB,1:1000); and anti‐vimentin (Abcam, ab8978; IHC, 1:50; WB, 1:1000). .. Secondary antibodies used for IF were: donkey anti‐goat Alexa‐Fluor‐488, donkey anti‐rabbit Alexa‐Fluor‐488, donkey anti‐rabbit Alexa‐Fluor‐568, donkey anti‐mouse Alexa‐Fluor‐568 and chicken anti‐mouse Alexa‐Fluor‐488 conjugated antibodies from Invitrogen (1:1000).

Labeling:

Article Title: Adjuvant therapy for T3N0 rectal cancer in the total mesorectal excision era- identification of the high risk patients
Article Snippet: .. Sections of 4-mm thickness were stained with hematoxylin-and-eosin for histologic diagnosis and then labeled with six primary antibodies: anti-epidermal growth factor receptor (EGFR) (Clone H11, code M3563, DAKO; dilution 1:100), anti-P21 (Clone NCC-RAS-001, code M0637, DAKO; dilution, 1:100), anti-Her-2 (Clone: PN2A, code M7269, DAKO; dilution 1:400), anti-P53 (Clone: DO-7, code M7001, DAKO; dilution, 1:50), anti-CD44v6 (Clone: VFF-7, code M0130, Antibody Diagnostica Inc; dilution 1:50), and anti-proliferating cell nuclear antigen (PCNA) (Clone: PC 10, code M 0879, DAKO; dilution 1:300). ..

Incubation:

Article Title: Prevention of diabetes-promoted colorectal cancer by (n-3) polyunsaturated fatty acids and (n-3) PUFA mimetic
Article Snippet: .. The slides were then incubated overnight with primary antibodies as indicated: anti-proliferating cell nuclear antigen (PCNA) (M0879, Dako Corp. Glostrup, Denmark) (used at 1:200 dilution); anti–E-cadherin (610181, BD Biosciences) (used at 1:100 dilution); monoclonal mouse anti-human HNF-4α (ab41898; Abcam, Cambridge, UK) (used at 1:200 dilution); and monoclonal mouse anti-human β-catenin (Santa Cruz Biotechnology, Dallas, TX) (used at 1:100 dilution). .. Following overnight incubation with primary antibodies the slides were washed with PBST, were further incubated with the protein blocking solution for 20 min., followed by incubating with respective secondary antibody: goat anti-mouse and goat anti-rabbit antibodies (Jackson Laboratory, Bar Harbor, ME) (used at 1:200 dilution).

Microscopy:

Article Title: Amelioration of diabesity-induced colorectal ontogenesis by omega-3 fatty acids in mice
Article Snippet: .. Sections (5 μm) on Superfrost Plus microscope slides (D-38116; Menzel, Braunschweig, Germany) were exposed to the following primary antibodies: anti-proliferating cell nuclear antigen (PCNA) (M0879; Dako, Glostrup, Denmark) (1:200 dilution); anti-E-cadherin (610181; BD Biosciences) (1:100 dilution); monoclonal mouse anti-human HNF-4α (ab41898; Abcam, Cambridge, UK) (1:200 dilution); monoclonal mouse anti-human β-catenin (1:100 dilution) (Santa Cruz Biotechnology, CA); polyclonal rabbit anti-aquaporin-8 (AQP8) (H-85; Santa Cruz Biotechnology) (1:300 dilution); and anti-MUC2 antibody (ab11197; Abcam) (1:200 dilution). .. The secondary antibodies used were goat anti-mouse or goat anti-rabbit (Jackson Laboratory) (1:200 dilution).

Western Blot:

Article Title: Pericardial patch venoplasty heals via attraction of venous progenitor cells. Pericardial patch venoplasty heals via attraction of venous progenitor cells
Article Snippet: .. Primary and secondary antibodies Primary antibodies included: anti‐α ‐actin (Abcam, ab5694; IHC and IF, 1:100; WB, 1:1000); anti‐cleaved Caspase‐3 (Cell Signaling #9661; IHC, 1:50; WB, 1:1000); anti‐CD31 (Abcam, ab28364; IHC and IF, 1:50); anti‐CD34 (R & D, AF4117; IF, 1:100; WB, 1:1000); anti‐CD45 (Abcam, ab10558; IHC, 1:50; WB, 1:1000); anti‐CD68 (ED1; Abcam, ab31630; IHC, 1:200; WB, 1:1000); anti‐ COUP‐TFII (Novus biologicals, NBP1‐67885; IHC, 1:100); anti‐Eph‐B4 (Santa Cruz, sc‐5536; IF, 1:50); anti‐dll‐4 (santa cruz, sc‐18640, 1:50); anti‐Eph‐B4 (Abcam, ab76657; IHC and IF, 1:50); anti‐Ephrin‐B2 (Novus, NBP1‐48610; IHC, 1:50); anti‐Eph‐B4 (R & D, AF446; WB, 1:1000); anti‐GAPDH (Cell Signaling, 14C10; WB, 1:2000); anti‐Ki67 (Abcam, ab15580; IHC, 1:100); anti‐proliferating cell nuclear antigen (PCNA) (Dako monoclonal mouse Anti‐PCNA; IHC and IF, 1:100; WB, 1:1000); anti‐VEGFR2 (ABCAM, ab2349; IF, 1:100; WB,1:1000); and anti‐vimentin (Abcam, ab8978; IHC, 1:50; WB, 1:1000). .. Secondary antibodies used for IF were: donkey anti‐goat Alexa‐Fluor‐488, donkey anti‐rabbit Alexa‐Fluor‐488, donkey anti‐rabbit Alexa‐Fluor‐568, donkey anti‐mouse Alexa‐Fluor‐568 and chicken anti‐mouse Alexa‐Fluor‐488 conjugated antibodies from Invitrogen (1:1000).

Staining:

Article Title: Spontaneous carcinosarcoma originating from the renal pelvis ina rat
Article Snippet: .. The tissue sections were also stained immunohistochemically with mouse monoclonal anti-uroplakin III , (Nichirei Bioscience, Tokyo, Japan), anti-vimentin (Dako Japan, Tokyo, Japan), anti-desmin antibodies (Dako Japan), and anti-proliferating cell nuclear antigen (PCNA) (Dako Japan) using an Envision system (Dako Japan) and visualized with diaminobenzidine tetrahydrochloride as the chromogen. ..

Article Title: Adjuvant therapy for T3N0 rectal cancer in the total mesorectal excision era- identification of the high risk patients
Article Snippet: .. Sections of 4-mm thickness were stained with hematoxylin-and-eosin for histologic diagnosis and then labeled with six primary antibodies: anti-epidermal growth factor receptor (EGFR) (Clone H11, code M3563, DAKO; dilution 1:100), anti-P21 (Clone NCC-RAS-001, code M0637, DAKO; dilution, 1:100), anti-Her-2 (Clone: PN2A, code M7269, DAKO; dilution 1:400), anti-P53 (Clone: DO-7, code M7001, DAKO; dilution, 1:50), anti-CD44v6 (Clone: VFF-7, code M0130, Antibody Diagnostica Inc; dilution 1:50), and anti-proliferating cell nuclear antigen (PCNA) (Clone: PC 10, code M 0879, DAKO; dilution 1:300). ..

Binding Assay:

Article Title: Spontaneous extraskeletal osteosarcoma with various histological growth patterns in the abdominal wall of an ICR mouse
Article Snippet: .. The primary antibodies used for immunohistochemistry were as follows: mouse anti-cytokeratin (AE1/AE3, monoclonal, prediluted, Dako, Glostrup, Denmark), anti-vimentin (V9, monoclonal, 1:50, Dako), anti-smooth muscle actin (SMA) (1A4, monoclonal, 1:100, Dako) and anti-proliferating cell nuclear antigen (PCNA) (PC10, monoclonal, 1:200, Dako), and rabbit anti-osterix (polyclonal, 1:200, Abcam, Cambridge, UK), anti-desmin (polyclonal, prediluted, Dako), anti-S100 (polyclonal, 1:400, Dako), anti-glial fibrillary acidic protein (GFAP) (polyclonal, prediluted, Dako), anti-factor VIII-related antigen (polyclonal, prediluted, BioGenex Laboratories, Fremont, CA, USA) and anti-ionized calcium binding adaptor molecule 1 (Iba1) (polyclonal, 1:500, Wako Pure Chemical Industries, Osaka, Japan). ..

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    Agilent technologies pcna
    Immunohistochemical findings of the lungs at 3 days. The images in A and B are for <t>γH2AX,</t> and those in C and D are for <t>PCNA.</t> Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.
    Pcna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcna/product/Agilent technologies
    Average 94 stars, based on 176 article reviews
    Price from $9.99 to $1999.99
    pcna - by Bioz Stars, 2020-07
    94/100 stars
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    93
    Agilent technologies mouse monoclonal antibody anti proliferating cell nuclear antigen pcna m0879
    Immunohistochemical findings of the lungs at 3 days. The images in A and B are for <t>γH2AX,</t> and those in C and D are for <t>PCNA.</t> Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.
    Mouse Monoclonal Antibody Anti Proliferating Cell Nuclear Antigen Pcna M0879, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal antibody anti proliferating cell nuclear antigen pcna m0879/product/Agilent technologies
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal antibody anti proliferating cell nuclear antigen pcna m0879 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Agilent technologies mouse anti pcna
    Immunohistochemical findings of the lungs at 3 days. The images in A and B are for <t>γH2AX,</t> and those in C and D are for <t>PCNA.</t> Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.
    Mouse Anti Pcna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pcna/product/Agilent technologies
    Average 93 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    mouse anti pcna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    Agilent technologies anti pcna antibody
    Immunohistochemical findings of the lungs at 3 days. The images in A and B are for <t>γH2AX,</t> and those in C and D are for <t>PCNA.</t> Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.
    Anti Pcna Antibody, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pcna antibody/product/Agilent technologies
    Average 93 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    anti pcna antibody - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    Image Search Results


    Immunohistochemical findings of the lungs at 3 days. The images in A and B are for γH2AX, and those in C and D are for PCNA. Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.

    Journal: Journal of Toxicologic Pathology

    Article Title: γH2AX is immunohistochemically detectable until 7 days after exposure of N-bis (2-hydroxypropyl) nitrosamine (DHPN) in rat lung carcinogenesis

    doi: 10.1293/tox.2017-0066

    Figure Lengend Snippet: Immunohistochemical findings of the lungs at 3 days. The images in A and B are for γH2AX, and those in C and D are for PCNA. Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.

    Article Snippet: A γH2AX antibody was purchased from Cell Signaling Technology (Danvers, MA, USA) and PCNA (Clone PC10, Code No. M0879) was purchased from Agilent (Santa Clara, CA, USA).

    Techniques: Immunohistochemistry, Staining