anti proliferating cell nuclear antigen pcna  (Abcam)

 
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    Structured Review

    Abcam anti proliferating cell nuclear antigen pcna
    The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein <t>(BLM)</t> and proliferating cell nuclear antigen <t>(PCNA)</t> were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Interactome Analysis Reveals that Heterochromatin Protein 1γ (HP1γ) Is Associated with the DNA Damage Response Pathway"

    Article Title: Interactome Analysis Reveals that Heterochromatin Protein 1γ (HP1γ) Is Associated with the DNA Damage Response Pathway

    Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

    doi: 10.4143/crt.2014.294

    The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein (BLM) and proliferating cell nuclear antigen (PCNA) were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.
    Figure Legend Snippet: The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein (BLM) and proliferating cell nuclear antigen (PCNA) were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.

    Techniques Used: Transfection

    2) Product Images from "FoxM1 overexpression promotes cell proliferation and migration and inhibits apoptosis in hypopharyngeal squamous cell carcinoma resulting in poor clinical prognosis"

    Article Title: FoxM1 overexpression promotes cell proliferation and migration and inhibits apoptosis in hypopharyngeal squamous cell carcinoma resulting in poor clinical prognosis

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2017.4094

    Expression of FoxM1 in proliferating Fadu cells. (A and B) Fadu cells were serum-deprived for 72 h in advance. (A) Cell cycle distribution was analyzed by flow cytometry. (B) Statistical analysis revealed a gradual decrease in the proportion of cells in G1 phase, and an increase in cells in S phase. (C) As more cells transitioned into S phase, the protein levels of FoxM1 and PCNA were also increased. Data represent the mean ± standard deviation from three independent experiments. Data were analyzed with a Student's t-test. ^,* P
    Figure Legend Snippet: Expression of FoxM1 in proliferating Fadu cells. (A and B) Fadu cells were serum-deprived for 72 h in advance. (A) Cell cycle distribution was analyzed by flow cytometry. (B) Statistical analysis revealed a gradual decrease in the proportion of cells in G1 phase, and an increase in cells in S phase. (C) As more cells transitioned into S phase, the protein levels of FoxM1 and PCNA were also increased. Data represent the mean ± standard deviation from three independent experiments. Data were analyzed with a Student's t-test. ^,* P

    Techniques Used: Expressing, Flow Cytometry, Cytometry, Standard Deviation

    3) Product Images from "Role of P2X7R in the development and progression of pulmonary hypertension"

    Article Title: Role of P2X7R in the development and progression of pulmonary hypertension

    Journal: Respiratory Research

    doi: 10.1186/s12931-017-0603-0

    A-740003 decreased the expression of proliferating cell nuclear antigen (PCNA). Immunofluorescence co-staining of PCNA ( Red ) with α-SMA ( Green ) in the a sham, b P/MCT vehicle group, c P/MCT + CA group, d P/MCT + DA group and e negative control. f The mean percentage of PCNA-positive α-SMA cells (magnification × 40, bar = 50 μm). Data are the mean ± SD, n = 6. ** p
    Figure Legend Snippet: A-740003 decreased the expression of proliferating cell nuclear antigen (PCNA). Immunofluorescence co-staining of PCNA ( Red ) with α-SMA ( Green ) in the a sham, b P/MCT vehicle group, c P/MCT + CA group, d P/MCT + DA group and e negative control. f The mean percentage of PCNA-positive α-SMA cells (magnification × 40, bar = 50 μm). Data are the mean ± SD, n = 6. ** p

    Techniques Used: Expressing, Immunofluorescence, Staining, Negative Control

    4) Product Images from "Apoptotic Effects of Xanthium strumarium via PI3K/AKT/mTOR Pathway in Hepatocellular Carcinoma"

    Article Title: Apoptotic Effects of Xanthium strumarium via PI3K/AKT/mTOR Pathway in Hepatocellular Carcinoma

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2019/2176701

    Effects of XS-5 and XS-6 in HCC ex vivo tumor models. (a) Balb/c nude mice bearing Huh-7 HCC xenograft tumors were cut into small pieces of ∼2 mm, and each piece of tumor was maintained in culture media. Tumor spheroids cultured from xenograft tumor tissues were treated with XS-5 and XS-6 (100 μ g/ml) for 5 days. Immunostaining and hematoxylin and eosin staining for PCNA and cleaved caspase-3 were carried out. (b) Tumor spheroids were excised and processed for immunofluorescence for p-AKT and p-mTOR. Images were captured at 400X magnification. Data are represented as the mean ± SD ( ∗∗∗ p
    Figure Legend Snippet: Effects of XS-5 and XS-6 in HCC ex vivo tumor models. (a) Balb/c nude mice bearing Huh-7 HCC xenograft tumors were cut into small pieces of ∼2 mm, and each piece of tumor was maintained in culture media. Tumor spheroids cultured from xenograft tumor tissues were treated with XS-5 and XS-6 (100 μ g/ml) for 5 days. Immunostaining and hematoxylin and eosin staining for PCNA and cleaved caspase-3 were carried out. (b) Tumor spheroids were excised and processed for immunofluorescence for p-AKT and p-mTOR. Images were captured at 400X magnification. Data are represented as the mean ± SD ( ∗∗∗ p

    Techniques Used: Ex Vivo, Mouse Assay, Cell Culture, Immunostaining, Staining, Immunofluorescence

    5) Product Images from "Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1"

    Article Title: Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19092660

    Effects of UPM on structure of reconstructed human epidermis, and expression of PCNA and filaggrin genes in the reconstructed epidermis. ( A ) Reconstructed epidermis was incubated in medium containing 200 ppm of UPM for 96 h in the presence of SB203580 and PDTC and then subjected to hemoxylin and eosin (H and E) staining and immunohistochemical staining for PCNA ( B ) and filaggrin ( C ). UPM, urban particulate matter; SB, SB203580; PDTC, ammonium pyrrolidinedithiocarbamate.
    Figure Legend Snippet: Effects of UPM on structure of reconstructed human epidermis, and expression of PCNA and filaggrin genes in the reconstructed epidermis. ( A ) Reconstructed epidermis was incubated in medium containing 200 ppm of UPM for 96 h in the presence of SB203580 and PDTC and then subjected to hemoxylin and eosin (H and E) staining and immunohistochemical staining for PCNA ( B ) and filaggrin ( C ). UPM, urban particulate matter; SB, SB203580; PDTC, ammonium pyrrolidinedithiocarbamate.

    Techniques Used: Expressing, Incubation, Staining, Immunohistochemistry

    6) Product Images from "HOTAIR contributes to the growth of liver cancer via targeting miR-217"

    Article Title: HOTAIR contributes to the growth of liver cancer via targeting miR-217

    Journal: Oncology Letters

    doi: 10.3892/ol.2018.8341

    miR-217 was involved in the regulation of HOTAIR on cell proliferation and cycle arrest in HepG2 cells. HepG2 cells were transfected with HOTAIR siRNA lentiviral vectors or miR-217 inhibitors, or the combination. (A) The proliferation rate was measured by CCK-8 assay. (B) The cell number was assessed by optical microscope. (C) The protein expression of Ki67, PCNA, p27 and cyclin D1 was evaluated by western blotting. (D) The cell cycle arrest was evaluated by flow cytometry (**P
    Figure Legend Snippet: miR-217 was involved in the regulation of HOTAIR on cell proliferation and cycle arrest in HepG2 cells. HepG2 cells were transfected with HOTAIR siRNA lentiviral vectors or miR-217 inhibitors, or the combination. (A) The proliferation rate was measured by CCK-8 assay. (B) The cell number was assessed by optical microscope. (C) The protein expression of Ki67, PCNA, p27 and cyclin D1 was evaluated by western blotting. (D) The cell cycle arrest was evaluated by flow cytometry (**P

    Techniques Used: Transfection, CCK-8 Assay, Microscopy, Expressing, Western Blot, Flow Cytometry, Cytometry

    HOTAIR inhibition suppresses tumor growth in xenograft models. Xenograft mouse model was created by subcutaneous injection of HepG2 cells pretreated with or without HOTAIR siRNA lentiviral vectors to nude mouse. (A) Five primary tumors from each group were photographed after sacrifice at 30th day. (B) Tumor growth curves in two groups were shown every 6 days until the 30th day following cells injection. (C and D) The expression of HPTAIR and miR-217 in primary tumor tissues was measured by qRT-PCR. (E and F) The expression of HPTAIR and miR-217 in primary tumor tissues was measured by northern blotting. (G and H) The expression of Ki67 and PCNA in primary tumor tissues was visualized by immunohistochemistry (*P
    Figure Legend Snippet: HOTAIR inhibition suppresses tumor growth in xenograft models. Xenograft mouse model was created by subcutaneous injection of HepG2 cells pretreated with or without HOTAIR siRNA lentiviral vectors to nude mouse. (A) Five primary tumors from each group were photographed after sacrifice at 30th day. (B) Tumor growth curves in two groups were shown every 6 days until the 30th day following cells injection. (C and D) The expression of HPTAIR and miR-217 in primary tumor tissues was measured by qRT-PCR. (E and F) The expression of HPTAIR and miR-217 in primary tumor tissues was measured by northern blotting. (G and H) The expression of Ki67 and PCNA in primary tumor tissues was visualized by immunohistochemistry (*P

    Techniques Used: Inhibition, Injection, Expressing, Quantitative RT-PCR, Northern Blot, Immunohistochemistry

    HOTAIR inhibition suppressed the proliferation of HepG2 cells. HepG2 cells were infected with HOTAIR siRNA lentiviral vectors or the empty lentiviral vectors for 72 h. (A) The expression of HOTAIR was evaluated by qRT-RCR. (B) The proliferation rate was measured by CCK-8 assay. (C and D) The cell number was assessed by optical microscope. (E) The protein expression of two proliferation marker proteins Ki67 and PCNA was evaluated by western blotting. GAPDH was used as an internal control (*P
    Figure Legend Snippet: HOTAIR inhibition suppressed the proliferation of HepG2 cells. HepG2 cells were infected with HOTAIR siRNA lentiviral vectors or the empty lentiviral vectors for 72 h. (A) The expression of HOTAIR was evaluated by qRT-RCR. (B) The proliferation rate was measured by CCK-8 assay. (C and D) The cell number was assessed by optical microscope. (E) The protein expression of two proliferation marker proteins Ki67 and PCNA was evaluated by western blotting. GAPDH was used as an internal control (*P

    Techniques Used: Inhibition, Infection, Expressing, CCK-8 Assay, Microscopy, Marker, Western Blot

    7) Product Images from "Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1"

    Article Title: Negative Cellular Effects of Urban Particulate Matter on Human Keratinocytes Are Mediated by P38 MAPK and NF-κB-dependent Expression of TRPV 1

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19092660

    Effects of UPM on structure of reconstructed human epidermis, and expression of PCNA and filaggrin genes in the reconstructed epidermis. ( A ) Reconstructed epidermis was incubated in medium containing 200 ppm of UPM for 96 h in the presence of SB203580 and PDTC and then subjected to hemoxylin and eosin (H and E) staining and immunohistochemical staining for PCNA ( B ) and filaggrin ( C ). UPM, urban particulate matter; SB, SB203580; PDTC, ammonium pyrrolidinedithiocarbamate.
    Figure Legend Snippet: Effects of UPM on structure of reconstructed human epidermis, and expression of PCNA and filaggrin genes in the reconstructed epidermis. ( A ) Reconstructed epidermis was incubated in medium containing 200 ppm of UPM for 96 h in the presence of SB203580 and PDTC and then subjected to hemoxylin and eosin (H and E) staining and immunohistochemical staining for PCNA ( B ) and filaggrin ( C ). UPM, urban particulate matter; SB, SB203580; PDTC, ammonium pyrrolidinedithiocarbamate.

    Techniques Used: Expressing, Incubation, Staining, Immunohistochemistry

    8) Product Images from "Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Facilitate Tubular Epithelial Cell Dedifferentiation and Growth via Hepatocyte Growth Factor Induction"

    Article Title: Microvesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Facilitate Tubular Epithelial Cell Dedifferentiation and Growth via Hepatocyte Growth Factor Induction

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0121534

    c-Met inhibitor or MEK inhibitor addition abrogates tubular cell dedifferentiation and growth, as well as Erk1/2 signaling activation induced by the CM of MV-treated injured tubular cells. (A), (C) Densitometric analysis of vimentin, PCNA and p-Erk1/2 protein levels in tubular cells. c-Met inhibitor or MEK inhibitor addition completely abrogated the up-regulation of vimentin, PCNA and p-Erk1/2 protein expression induced by CM of damaged tubular cells treated with MVs. Values in the graph are expressed as densitometric ratios of vimentin/β-actin, PCNA/β-actin or p-Erk1/2/β-actin as fold changes compared with the control (CM of tubular cells treated with vehicle) (dotted line). *P
    Figure Legend Snippet: c-Met inhibitor or MEK inhibitor addition abrogates tubular cell dedifferentiation and growth, as well as Erk1/2 signaling activation induced by the CM of MV-treated injured tubular cells. (A), (C) Densitometric analysis of vimentin, PCNA and p-Erk1/2 protein levels in tubular cells. c-Met inhibitor or MEK inhibitor addition completely abrogated the up-regulation of vimentin, PCNA and p-Erk1/2 protein expression induced by CM of damaged tubular cells treated with MVs. Values in the graph are expressed as densitometric ratios of vimentin/β-actin, PCNA/β-actin or p-Erk1/2/β-actin as fold changes compared with the control (CM of tubular cells treated with vehicle) (dotted line). *P

    Techniques Used: Activation Assay, Expressing

    MV administration promotes tubular cell dedifferentiation and proliferation at 48 h post-injury, whereas cell apoptosis is inhibited. Representative micrographs showing vimentin, PCNA and TUNEL staining of tubular cells. Immuno-staining for vimentin and PCNA proteins, which are indictors for tubular cell dedifferentiation and cell proliferation, respectively, was employed. TUNEL staining was used to detect cell apoptosis. In contrast to the rats treated with vehicle or with RNase-MVs, the rats receiving MV treatment displayed more PCNA- and vimentin-positive stained tubular cells and fewer TUNEL-positive cells on kidney tissue sections. Magnification, ×40.
    Figure Legend Snippet: MV administration promotes tubular cell dedifferentiation and proliferation at 48 h post-injury, whereas cell apoptosis is inhibited. Representative micrographs showing vimentin, PCNA and TUNEL staining of tubular cells. Immuno-staining for vimentin and PCNA proteins, which are indictors for tubular cell dedifferentiation and cell proliferation, respectively, was employed. TUNEL staining was used to detect cell apoptosis. In contrast to the rats treated with vehicle or with RNase-MVs, the rats receiving MV treatment displayed more PCNA- and vimentin-positive stained tubular cells and fewer TUNEL-positive cells on kidney tissue sections. Magnification, ×40.

    Techniques Used: TUNEL Assay, Staining, Immunostaining

    9) Product Images from "KIFC1 promotes the proliferation of hepatocellular carcinoma in vitro and in vivo"

    Article Title: KIFC1 promotes the proliferation of hepatocellular carcinoma in vitro and in vivo

    Journal: Oncology Letters

    doi: 10.3892/ol.2019.10985

    Knockdown of KIFC1 reduces proliferation of Hep3B and SNU-475 cells. (A) Colony formation and (B) MTT assays examined Hep3B and SNU-475 cell lines transfected with control or KIFC1 shRNA plasmids. (C and D) Western blot analysis demonstrated that (C) Ki67 and (D) PCNA expression was downregulated in Hep3B and SNU-475 cells. Data are presented as mean ± standard deviation. *P
    Figure Legend Snippet: Knockdown of KIFC1 reduces proliferation of Hep3B and SNU-475 cells. (A) Colony formation and (B) MTT assays examined Hep3B and SNU-475 cell lines transfected with control or KIFC1 shRNA plasmids. (C and D) Western blot analysis demonstrated that (C) Ki67 and (D) PCNA expression was downregulated in Hep3B and SNU-475 cells. Data are presented as mean ± standard deviation. *P

    Techniques Used: MTT Assay, Transfection, shRNA, Western Blot, Expressing, Standard Deviation

    10) Product Images from "Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy"

    Article Title: Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy

    Journal: Scientific Reports

    doi: 10.1038/srep15807

    Amiodarone enhanced liver growth and hepatocyte proliferation in the liver regeneration after PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 168 h. Liver tissues were harvested at 0–168 h after surgery. Liver-to-body-weight ratios were calculated ( A ). Liver sections at 24 h after the sham operation, PHx with Veh, AD, or CQ were stained with H E; original magnification, 400X ( B ). Representative immunohistochemical staining of Ki67 is shown ( C ). The percentage of Ki67-positive nuclei in hepatocyte was counted under low-power fields (200 ×) in 15 random sections from at least six different mice ( D ). Liver tissues were harvested at 0 h, 12 h, 24 h, or 48 h after surgery, and the tissue extracts were analyzed for PCNA, cyclin A, B, D1, E, p21, TGF-β1, and β-actin protein by Western blotting ( E,F ). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. # P
    Figure Legend Snippet: Amiodarone enhanced liver growth and hepatocyte proliferation in the liver regeneration after PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 168 h. Liver tissues were harvested at 0–168 h after surgery. Liver-to-body-weight ratios were calculated ( A ). Liver sections at 24 h after the sham operation, PHx with Veh, AD, or CQ were stained with H E; original magnification, 400X ( B ). Representative immunohistochemical staining of Ki67 is shown ( C ). The percentage of Ki67-positive nuclei in hepatocyte was counted under low-power fields (200 ×) in 15 random sections from at least six different mice ( D ). Liver tissues were harvested at 0 h, 12 h, 24 h, or 48 h after surgery, and the tissue extracts were analyzed for PCNA, cyclin A, B, D1, E, p21, TGF-β1, and β-actin protein by Western blotting ( E,F ). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. # P

    Techniques Used: Mouse Assay, Injection, Staining, Immunohistochemistry, Western Blot

    Inhibition of autophagy reduced liver growth and hepatocyte proliferation in the early phase of liver regeneration after PHx. Wild-type mice were given control or Atg7-specific siRNA for 48 h before treatment with PHx. Liver tissues were harvested at 24 h after surgery and tissue extracts were analyzed for Atg7, LC3-II, and β-actin by Western blotting ( A ). The liver-to-body-weight ratios were calculated ( B ). Representative immunohistochemical staining of Ki67 is shown. Scale bar, 50 μm ( C ). The percentage of Ki67-positive nuclei in hepatocytes was counted in low-power field (200X) in 15 random sections from 3 different mice ( D ). Tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( E ). Immunohistochemical staining of senescence-associated β-galactosidase (SA-β-gal) in hepatocytes. Scale bar, 100 μm ( F ). Fold-changes in IL-6 ( G ) and IL-8 ( H ) mRNA expression at 24 h after 70% PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh) or chloroquine (CQ) at 0.5 h before PHx or the sham operation and then once per day until 48 h. Liver tissues were harvested at 0–48 h after surgery and tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( I,J ). The values are shown as the mean ± SD in the bar graph and compared using Student’s t test. #P
    Figure Legend Snippet: Inhibition of autophagy reduced liver growth and hepatocyte proliferation in the early phase of liver regeneration after PHx. Wild-type mice were given control or Atg7-specific siRNA for 48 h before treatment with PHx. Liver tissues were harvested at 24 h after surgery and tissue extracts were analyzed for Atg7, LC3-II, and β-actin by Western blotting ( A ). The liver-to-body-weight ratios were calculated ( B ). Representative immunohistochemical staining of Ki67 is shown. Scale bar, 50 μm ( C ). The percentage of Ki67-positive nuclei in hepatocytes was counted in low-power field (200X) in 15 random sections from 3 different mice ( D ). Tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( E ). Immunohistochemical staining of senescence-associated β-galactosidase (SA-β-gal) in hepatocytes. Scale bar, 100 μm ( F ). Fold-changes in IL-6 ( G ) and IL-8 ( H ) mRNA expression at 24 h after 70% PHx. Wild-type mice were intraperitoneally injected with vehicle (Veh) or chloroquine (CQ) at 0.5 h before PHx or the sham operation and then once per day until 48 h. Liver tissues were harvested at 0–48 h after surgery and tissue extracts were analyzed for PCNA, cyclin D1, TGF-β1, and β-actin by Western blotting ( I,J ). The values are shown as the mean ± SD in the bar graph and compared using Student’s t test. #P

    Techniques Used: Inhibition, Mouse Assay, Western Blot, Immunohistochemistry, Staining, Expressing, Injection

    11) Product Images from "Reduction of atherosclerotic lesions in rabbits treated with etoposide associated with cholesterol-rich nanoemulsions"

    Article Title: Reduction of atherosclerotic lesions in rabbits treated with etoposide associated with cholesterol-rich nanoemulsions

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S24048

    Immunohistochemistry of artery tissues from rabbits treated with saline solution (control group) or LDE-etoposide. Photomicrographs of diaminobenzidine chromogen immunostaining for MMP9, IL-1β, TNF-α, PCNA, topoisomerase IIα, tubulin. Magnifications: 100×. Abbreviations: IL, interleukin; LDE, cholesterol-rich nanoemulsion; MMP9, matrix metallopeptidase 9; PCNA, proliferating cell nuclear antigen; TNF, tumor necrosis factor.
    Figure Legend Snippet: Immunohistochemistry of artery tissues from rabbits treated with saline solution (control group) or LDE-etoposide. Photomicrographs of diaminobenzidine chromogen immunostaining for MMP9, IL-1β, TNF-α, PCNA, topoisomerase IIα, tubulin. Magnifications: 100×. Abbreviations: IL, interleukin; LDE, cholesterol-rich nanoemulsion; MMP9, matrix metallopeptidase 9; PCNA, proliferating cell nuclear antigen; TNF, tumor necrosis factor.

    Techniques Used: Immunohistochemistry, Immunostaining

    12) Product Images from "Mesenchymal stem cells alleviate acute kidney injury via miR-107-mediated regulation of ribosomal protein S19"

    Article Title: Mesenchymal stem cells alleviate acute kidney injury via miR-107-mediated regulation of ribosomal protein S19

    Journal: Annals of Translational Medicine

    doi: 10.21037/atm.2019.11.89

    Inhibition of microRNA (miR)-107 improves cell proliferation and hinders apoptosis. (A) miR-107 was inhibited in HK-2 cells transfected with inhibitors. HK-2 cells treated with NC were adopted as controls; (B) the protein expression levels of RPS19, PCNA, Bax and, Bcl-2 in HK-2 cells transfected with NC or inhibitor were analyzed by Western blot. Compared with NC transfection, transfection with miR-107 inhibitor increased the expression of RPS19. The protein expression level of PCNA in the inhibitor group increased, and the Bax-Bcl-2 ratio decreased. *, P
    Figure Legend Snippet: Inhibition of microRNA (miR)-107 improves cell proliferation and hinders apoptosis. (A) miR-107 was inhibited in HK-2 cells transfected with inhibitors. HK-2 cells treated with NC were adopted as controls; (B) the protein expression levels of RPS19, PCNA, Bax and, Bcl-2 in HK-2 cells transfected with NC or inhibitor were analyzed by Western blot. Compared with NC transfection, transfection with miR-107 inhibitor increased the expression of RPS19. The protein expression level of PCNA in the inhibitor group increased, and the Bax-Bcl-2 ratio decreased. *, P

    Techniques Used: Inhibition, Transfection, Expressing, Western Blot

    miR-107 is downregulated and RPS19 is upregulated in HK-2 cells cocultured with MSCs. (A) Expression of miR-107 in various groups of HK-2 cells. Cells in the MSC group were pretreated with cisplatin and cocultured in Transwell plates for 6 h; normal HK-2 cells served as a control. (B) Western blot analysis indicated that the protein expression of RPS19 was downregulated in the cisplatin group and upregulated in the MSC group. Western blot analysis suggested that the expression of PCNA in the cisplatin group was higher than that in the MSC group. In contrast, the ratio of Bax to Bcl-2 was increased in the cisplatin group and reduced in the MSC group. *, P
    Figure Legend Snippet: miR-107 is downregulated and RPS19 is upregulated in HK-2 cells cocultured with MSCs. (A) Expression of miR-107 in various groups of HK-2 cells. Cells in the MSC group were pretreated with cisplatin and cocultured in Transwell plates for 6 h; normal HK-2 cells served as a control. (B) Western blot analysis indicated that the protein expression of RPS19 was downregulated in the cisplatin group and upregulated in the MSC group. Western blot analysis suggested that the expression of PCNA in the cisplatin group was higher than that in the MSC group. In contrast, the ratio of Bax to Bcl-2 was increased in the cisplatin group and reduced in the MSC group. *, P

    Techniques Used: Expressing, Western Blot

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    Immunohistochemistry:

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    Article Snippet: .. IHC staining of patient tissue sections was performed using the anti-E2F7 (Santa Cruz), anti-Ki67 (Abcam), anti-PCNA (Abcam), anti-E-cadherin (Cell Signalling Technology), anti-N-cadherin (Cell Signalling Technology), anti-vimentin (Cell Signalling Technology). .. After washing three times with PBS, the sections were incubated with the secondary antibodies at room temperature for 1 h. Subsequently, the sections were immersed in DAB for 5–10 min and counterstained with 10% Mayer’s hematoxylin.

    Article Title: Ubiquitin specific peptidase 5 promotes ovarian cancer cell proliferation through deubiquitinating HDAC2
    Article Snippet: .. Parts of the xenografts were immediately frozen and kept at −80 °C for further western blot analysis, and remaining tissues were processed for formalin-fixed paraffin-embedded-sectioned and immunohistochemistry staining with anti-PCNA (Abcam). .. Coimmunoprecipitation assays Cell lysates were incubated with anti-USP5 (Abcam), anti-HDAC2 (Abcam) or control IgG (Santa Cruz Biotech., Santa Cruz, CA, USA) for 1 h at 4°C with rotation, and then with protein A/G-agarose (Santa Cruz Biotech.) for 2 h at 4°C with rotation.

    Immunostaining:

    Article Title: DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression
    Article Snippet: .. For immunostaining, the following antibodies were used: anti-BLM (rabbit, polyclonal; ab476, Abcam), anti-BRCA2 (mouse; mAb Ab-1, Calbiochem), anti-EXO1 (rabbit, polyclonal; GTX109891, GeneTex), anti–HA-tag (mouse; mAb peroxidase-conjugated 3F10, Roche), anti-HLTF (rabbit, polyclonal; A300-229A, Bethyl), anti-MRE11 (rabbit, polyclonal; NB100-142, Novus), anti-p21 (mouse; mAb SX118, BD Biosciences), anti-p53 (mouse; mAb DO1, Calbiochem), anti-PARI (rabbit, polyclonal; ab107308, Abcam), anti-PCNA (mouse; mAb PC10, Abcam), anti-POLη (rabbit, polyclonal; NB100-60424, Novus), anti-POLι (rabbit, polyclonal; ab1324, Abcam), anti-RAD18 (rabbit, polyclonal; A301-340A, Bethyl), anti-RAD51 (rabbit, polyclonal; H-92, Santa-Cruz Biotechnology), anti-SMARCAL1 (rabbit, polyclonal; A301-616A, Bethyl), anti-WRN (rabbit, polyclonal; H-300, Santa Cruz Biotechnology), and anti-ZRANB3 (rabbit, polyclonal; A303-033A, Bethyl). .. To verify equal amounts of proteins, the membranes were prestained with Ponceau Red (Sigma) or reincubated with anti–α-actin antibody (goat, polyclonal; I-19, Santa Cruz Biotechnology) or anti-GAPDH (mouse mAb; Abcam).

    Incubation:

    Article Title: Long Non-coding RNA Profiling Reveals an Abundant MDNCR that Promotes Differentiation of Myoblasts by Sponging miR-133a
    Article Snippet: .. The membrane was then incubated overnight with primary antibodies specific for anti-MyoD, anti-MyHC, anti-MyoG, anti-PCNA, anti-CyclinD1 (Abcam, Cambridge, England), and anti-β-actin (Sungene Biotech, Tianjin, China) at 4°C. .. The PVDF membrane was washed three times with tris saline with tween (TBST) buffer and then incubated with secondary antibody for 2 hr at room temperature. β-Actin was used as the internal control with a secondary antibody that was horseradish peroxidase (HRP)-labeled anti-mouse immunoglobulin G (IgG) (Sungene Biotech, China).

    Article Title: Administration of Steamed and Freeze-Dried Mature Silkworm Larval Powder Prevents Hepatic Fibrosis and Hepatocellular Carcinogenesis by Blocking TGF-β/STAT3 Signaling Cascades in Rats
    Article Snippet: .. After blocking by blocking solution for 1 h, the sections were incubated 2 h at room temperature with primary antibodies against PCNA (1:20,000, ab29, Abcam, Cambridge, UK) or Ki67 (1:250, ab15580, Abcam, Cambridge, UK). .. After incubation, the slices were washed three times for 5 min with PBS.

    Blocking Assay:

    Article Title: Biomimetic Culture Reactor for Whole-Lung Engineering
    Article Snippet: .. Antigen retrieval was performed in 1 mM EDTA, 10 mM Tris, and 0.05% Tween-20 buffer at pH 9 for 20 min at 75°C and allowed to cool to room temperature for 20 min. After blocking sections with PBS containing 10% FBS and 0.2% Triton X-100 for 45 min, primary antibodies were used against CCSP (07-623, 1:100; Millipore), Pro-SPC (ab40879; Abcam), AQP-5 (AB3559-50UL, 1:500; Millipore), PCNA (ab29, 1:500; Abcam), and PECAM-1 (ab28364, 1:100; Abcam) for 2 h at room temperature. ..

    Article Title: Administration of Steamed and Freeze-Dried Mature Silkworm Larval Powder Prevents Hepatic Fibrosis and Hepatocellular Carcinogenesis by Blocking TGF-β/STAT3 Signaling Cascades in Rats
    Article Snippet: .. After blocking by blocking solution for 1 h, the sections were incubated 2 h at room temperature with primary antibodies against PCNA (1:20,000, ab29, Abcam, Cambridge, UK) or Ki67 (1:250, ab15580, Abcam, Cambridge, UK). .. After incubation, the slices were washed three times for 5 min with PBS.

    Western Blot:

    Article Title: Ubiquitin specific peptidase 5 promotes ovarian cancer cell proliferation through deubiquitinating HDAC2
    Article Snippet: .. Parts of the xenografts were immediately frozen and kept at −80 °C for further western blot analysis, and remaining tissues were processed for formalin-fixed paraffin-embedded-sectioned and immunohistochemistry staining with anti-PCNA (Abcam). .. Coimmunoprecipitation assays Cell lysates were incubated with anti-USP5 (Abcam), anti-HDAC2 (Abcam) or control IgG (Santa Cruz Biotech., Santa Cruz, CA, USA) for 1 h at 4°C with rotation, and then with protein A/G-agarose (Santa Cruz Biotech.) for 2 h at 4°C with rotation.

    Staining:

    Article Title: MicroRNA-30a-5p inhibits gallbladder cancer cell proliferation, migration and metastasis by targeting E2F7
    Article Snippet: .. IHC staining of patient tissue sections was performed using the anti-E2F7 (Santa Cruz), anti-Ki67 (Abcam), anti-PCNA (Abcam), anti-E-cadherin (Cell Signalling Technology), anti-N-cadherin (Cell Signalling Technology), anti-vimentin (Cell Signalling Technology). .. After washing three times with PBS, the sections were incubated with the secondary antibodies at room temperature for 1 h. Subsequently, the sections were immersed in DAB for 5–10 min and counterstained with 10% Mayer’s hematoxylin.

    Article Title: Ubiquitin specific peptidase 5 promotes ovarian cancer cell proliferation through deubiquitinating HDAC2
    Article Snippet: .. Parts of the xenografts were immediately frozen and kept at −80 °C for further western blot analysis, and remaining tissues were processed for formalin-fixed paraffin-embedded-sectioned and immunohistochemistry staining with anti-PCNA (Abcam). .. Coimmunoprecipitation assays Cell lysates were incubated with anti-USP5 (Abcam), anti-HDAC2 (Abcam) or control IgG (Santa Cruz Biotech., Santa Cruz, CA, USA) for 1 h at 4°C with rotation, and then with protein A/G-agarose (Santa Cruz Biotech.) for 2 h at 4°C with rotation.

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  • 92
    Abcam anti proliferating cell nuclear antigen pcna
    The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein <t>(BLM)</t> and proliferating cell nuclear antigen <t>(PCNA)</t> were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.
    Anti Proliferating Cell Nuclear Antigen Pcna, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam rabbit anti pcna polyclonal antiserum
    <t>PCNA</t> immunolabeling during the ouabain-induced retinal regeneration response. Frozen retinal sections were double immunolabeled with an anti-PCNA monoclonal antibody (green), a marker of proliferating cells, and rabbit anti-rod opsin <t>polyclonal</t> antisera (red), which marks the ROS. A , Control section possessing very few PCNA-labeled cells and an intact ROS. B , At 1 dpi, the retinal section possessed an increased number of round, punctate PCNA-positive nuclei randomly distributed in the inner retinal cell layers. C , Large numbers of PCNA-labeled cells were localized to the INL at 3 dpi. Many of these nuclei had a morphology that is consistent with Müller glia (arrowheads). D , E , Between 5 and 7 dpi, a large proliferative response spanning the entire retina is characterized by columnar clusters of PCNA-labeled cells, with fusiform morphology (arrows). F , G , At 14 through 21 dpi, the PCNA-labeled cells extend into the ONL. H , A few PCNA-positive cells are identified in the ONL at 60 dpi. The ROS remains intact throughout the time course. I , Graph of the number of PCNA-labeled cells in the dorsal retina ( y -axis) versus the days after ouabain injection ( x -axis). Ctrl., Control. Error bars represent SD ( n = 4 retinas per time point). Scale bar, 75 μm.
    Rabbit Anti Pcna Polyclonal Antiserum, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam mouse anti pcna monoclonal
    Close proximity of HP1a with Mcm10, RFC140 or <t>dpolε255</t> in eye imaginal discs. Eye imaginal discs of third instar larvae from Canton S were labeled with EdU (green) ( A, E, I, M and Q ) and performed with PLA assays to examine close proximity between two target proteins ( B, F, J, N and R ). PLA examines close proximity between dpolα and Mcm10 ( C and D ), HP1a and Mcm10 ( G and H ), HP1a and RFC140 ( K and L ), HP1a and dpolε255 ( O and P ) in the S-phase cells labeled with EdU and in the posterior regions to MF where photoreceptor cells differentiate. No PLA signal was detected between <t>PCNA</t> and Mcm10 in eye imaginal discs ( Q–T ). Enlarged images of the indicated regions (red rectangles) in C, G, K, O and S are shown in D, H, L, P and T, respectively. Scale bars show 10, 30 or 40 μm. (a) indicates anterior, (p) indicates posterior. Blue arrows indicate morphogenetic furrow (MF).
    Mouse Anti Pcna Monoclonal, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein (BLM) and proliferating cell nuclear antigen (PCNA) were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.

    Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

    Article Title: Interactome Analysis Reveals that Heterochromatin Protein 1γ (HP1γ) Is Associated with the DNA Damage Response Pathway

    doi: 10.4143/crt.2014.294

    Figure Lengend Snippet: The function of heterochromatin protein 1γ (HP1γ) in the DNA damage response pathway. (A) Bloom syndrome protein (BLM) and proliferating cell nuclear antigen (PCNA) were associated with endogenous HP1γ proteins. BLM and PCNA were coimmunoprecipitated with anti-HP1γ antibodies using HEK293T cell lysates and immunoblotted with indicated antibodies. (B) HeLa cells were treated with 50 μM of etoposide or vehicle (dimethyl sulfoxide, DMSO) for 6 hours and immunostained with the indicated antibodies. (C) HeLa cells were transfected with pSUPER-siHP1γ and immunostained with the indicated antibodies. (D) Nonproportional Venn diagram showing subsets of HP1γ-interacting proteins functionally related to DNA damage response pathways. The numbers in five subsets represent proteins that are interacting with HP1γ and functions in DNA damage response. IB, immunoblot; IP, immunoprecipitate.

    Article Snippet: The antibodies used for immunoblotting were as follows: anti-phospho-H3 Ser 10 (EMD Millipore), anti-Bloom syndrome protein (BLM) (Abcam, Cambridge, MA), and anti-proliferating cell nuclear antigen (PCNA) (Abcam).

    Techniques: Transfection

    Expression of FoxM1 in proliferating Fadu cells. (A and B) Fadu cells were serum-deprived for 72 h in advance. (A) Cell cycle distribution was analyzed by flow cytometry. (B) Statistical analysis revealed a gradual decrease in the proportion of cells in G1 phase, and an increase in cells in S phase. (C) As more cells transitioned into S phase, the protein levels of FoxM1 and PCNA were also increased. Data represent the mean ± standard deviation from three independent experiments. Data were analyzed with a Student's t-test. ^,* P

    Journal: International Journal of Oncology

    Article Title: FoxM1 overexpression promotes cell proliferation and migration and inhibits apoptosis in hypopharyngeal squamous cell carcinoma resulting in poor clinical prognosis

    doi: 10.3892/ijo.2017.4094

    Figure Lengend Snippet: Expression of FoxM1 in proliferating Fadu cells. (A and B) Fadu cells were serum-deprived for 72 h in advance. (A) Cell cycle distribution was analyzed by flow cytometry. (B) Statistical analysis revealed a gradual decrease in the proportion of cells in G1 phase, and an increase in cells in S phase. (C) As more cells transitioned into S phase, the protein levels of FoxM1 and PCNA were also increased. Data represent the mean ± standard deviation from three independent experiments. Data were analyzed with a Student's t-test. ^,* P

    Article Snippet: After blocking in 5% skimmed milk in Tris-buffered saline Tween-20 (TBST) for 2 h, the membranes were incubated with the primary antibodies overnight at 4°C [anti-FoxM1 (1:1,000; Bioworld Technology), anti-GAPDH (1:8,000; Abways Technology, Wynne, AR, USA), anti-proliferating cell nuclear antigen (PCNA) (#ab2426, 1:2,000; Abcam), anti-cyclin A1 (#sc-751; 1:300; Santa Cruz Biotechnology), anti-E-cadherin (#RLT1453, 1:300; Suzhou Ruiying Biological Technology, Co., Ltd., Jiaozuo, China) and anti-vimentin (#RLT4879, 1:300; Suzhou Ruiying Biological Technology)], followed by incubation for 1 h with the corresponding secondary antibody (1:1,000) at room temperature.

    Techniques: Expressing, Flow Cytometry, Cytometry, Standard Deviation

    PCNA immunolabeling during the ouabain-induced retinal regeneration response. Frozen retinal sections were double immunolabeled with an anti-PCNA monoclonal antibody (green), a marker of proliferating cells, and rabbit anti-rod opsin polyclonal antisera (red), which marks the ROS. A , Control section possessing very few PCNA-labeled cells and an intact ROS. B , At 1 dpi, the retinal section possessed an increased number of round, punctate PCNA-positive nuclei randomly distributed in the inner retinal cell layers. C , Large numbers of PCNA-labeled cells were localized to the INL at 3 dpi. Many of these nuclei had a morphology that is consistent with Müller glia (arrowheads). D , E , Between 5 and 7 dpi, a large proliferative response spanning the entire retina is characterized by columnar clusters of PCNA-labeled cells, with fusiform morphology (arrows). F , G , At 14 through 21 dpi, the PCNA-labeled cells extend into the ONL. H , A few PCNA-positive cells are identified in the ONL at 60 dpi. The ROS remains intact throughout the time course. I , Graph of the number of PCNA-labeled cells in the dorsal retina ( y -axis) versus the days after ouabain injection ( x -axis). Ctrl., Control. Error bars represent SD ( n = 4 retinas per time point). Scale bar, 75 μm.

    Journal: The Journal of Neuroscience

    Article Title: Regeneration of Inner Retinal Neurons after Intravitreal Injection of Ouabain in Zebrafish

    doi: 10.1523/JNEUROSCI.5317-06.2007

    Figure Lengend Snippet: PCNA immunolabeling during the ouabain-induced retinal regeneration response. Frozen retinal sections were double immunolabeled with an anti-PCNA monoclonal antibody (green), a marker of proliferating cells, and rabbit anti-rod opsin polyclonal antisera (red), which marks the ROS. A , Control section possessing very few PCNA-labeled cells and an intact ROS. B , At 1 dpi, the retinal section possessed an increased number of round, punctate PCNA-positive nuclei randomly distributed in the inner retinal cell layers. C , Large numbers of PCNA-labeled cells were localized to the INL at 3 dpi. Many of these nuclei had a morphology that is consistent with Müller glia (arrowheads). D , E , Between 5 and 7 dpi, a large proliferative response spanning the entire retina is characterized by columnar clusters of PCNA-labeled cells, with fusiform morphology (arrows). F , G , At 14 through 21 dpi, the PCNA-labeled cells extend into the ONL. H , A few PCNA-positive cells are identified in the ONL at 60 dpi. The ROS remains intact throughout the time course. I , Graph of the number of PCNA-labeled cells in the dorsal retina ( y -axis) versus the days after ouabain injection ( x -axis). Ctrl., Control. Error bars represent SD ( n = 4 retinas per time point). Scale bar, 75 μm.

    Article Snippet: The sections were incubated with either a 1:1000 dilution of anti-proliferating cell nuclear antigen (PCNA) monoclonal antibody (clone PC10; Sigma, St. Louis, MO), a 1:500 dilution of rabbit anti-PCNA polyclonal antiserum (Abcam, Cambridge, MA), a 1:1500 dilution of rabbit anti-GFP polyclonal antiserum (Abcam), or a 1:50 dilution of mouse anti-zn5 monoclonal antibody (Zebrafish International Resource Center, Eugene, OR) in PBS/2% normal goat serum/0.2% Triton X-100/1% DMSO.

    Techniques: Immunolabeling, Marker, Labeling, Injection

    Close proximity of HP1a with Mcm10, RFC140 or dpolε255 in eye imaginal discs. Eye imaginal discs of third instar larvae from Canton S were labeled with EdU (green) ( A, E, I, M and Q ) and performed with PLA assays to examine close proximity between two target proteins ( B, F, J, N and R ). PLA examines close proximity between dpolα and Mcm10 ( C and D ), HP1a and Mcm10 ( G and H ), HP1a and RFC140 ( K and L ), HP1a and dpolε255 ( O and P ) in the S-phase cells labeled with EdU and in the posterior regions to MF where photoreceptor cells differentiate. No PLA signal was detected between PCNA and Mcm10 in eye imaginal discs ( Q–T ). Enlarged images of the indicated regions (red rectangles) in C, G, K, O and S are shown in D, H, L, P and T, respectively. Scale bars show 10, 30 or 40 μm. (a) indicates anterior, (p) indicates posterior. Blue arrows indicate morphogenetic furrow (MF).

    Journal: Nucleic Acids Research

    Article Title: Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation

    doi: 10.1093/nar/gkw1174

    Figure Lengend Snippet: Close proximity of HP1a with Mcm10, RFC140 or dpolε255 in eye imaginal discs. Eye imaginal discs of third instar larvae from Canton S were labeled with EdU (green) ( A, E, I, M and Q ) and performed with PLA assays to examine close proximity between two target proteins ( B, F, J, N and R ). PLA examines close proximity between dpolα and Mcm10 ( C and D ), HP1a and Mcm10 ( G and H ), HP1a and RFC140 ( K and L ), HP1a and dpolε255 ( O and P ) in the S-phase cells labeled with EdU and in the posterior regions to MF where photoreceptor cells differentiate. No PLA signal was detected between PCNA and Mcm10 in eye imaginal discs ( Q–T ). Enlarged images of the indicated regions (red rectangles) in C, G, K, O and S are shown in D, H, L, P and T, respectively. Scale bars show 10, 30 or 40 μm. (a) indicates anterior, (p) indicates posterior. Blue arrows indicate morphogenetic furrow (MF).

    Article Snippet: The blotted membranes were blocked in Tris Buffer Saline Tween 20 (TBST) pH 7.6 (10 mM Tris–HCl, 150 mM NaCl and 0.1% Tween20) containing 5% non-fat milk for 1h at 25°C and then incubated with rabbit anti-Mcm10 polyclonal (diluted 1:1000 ( )), rabbit anti-dpolε255 polyclonal (1:5000 ( )), rabbit anti-RFC140 polyclonal (1:1000 ( )), mouse anti-PCNA monoclonal (1:5000, Abcam), mouse anti-HP1a monoclonal (1:10 000, DSHB), mouse anti-Halotag monoclonal (1:5000, Promega), mouse anti-His6 monoclonal (1:1000, Roche) in TBST containing 1% BSA for 16h at 4°C.

    Techniques: Labeling, Proximity Ligation Assay