Journal: BMC Neuroscience

Article Title: Astrocytes infected with Chlamydia pneumoniae demonstrate altered expression and activity of secretases involved in the generation of β-amyloid found in Alzheimer disease

doi: 10.1186/s12868-019-0489-5

Figure Lengend Snippet: Chlamydia pneumoniae infection of human astrocytes alters the transcript expression of AD-related genes. Gene transcripts from Cpn -infected and uninfected cells analyzed at all four timepoints post-infection revealed significant fold changes in genes closely related to AD pathology. The fold changes of fourteen genes implicated in known pathways of AD pathology are presented in a . Histograms of fold changes of these AD-associated genes are presented in b . All expression data was normalized to β-actin and Cpn -infected and uninfected cDNA samples were repeated in biological ( N = 3) and technical triplicate for each timepoint. Asterisk indicates p < 0.05. ADAM10, A disintegrin and metalloproteinase 10; APH1A, anterior pharynx defective protein 1A; APOE, apolipoprotein E; APP, amyloid precursor protein; BACE1, βAPP-cleaving enzyme 1; GSK3B, glucogen synthase kinase 3-β; IL1A, interleukin 1α; LPL, lipoprotein lipase; lipoprotein receptor-related protein 1, LRP1; MAP2, microtubule associated protein 2; MAPT, microtubule associated protein tau; NCSTN, nicastrin; PSEN1, presenilin-1, PSEN2, presenilin-2

Article Snippet: Cells grown on sterile 18.5 mm glass coverslips were incubated with the following primary antibodies: anti-Aβ 1-42 at 1:500 (Synaptic Systems, 218703); anti-ADAM 10 at 1:100 (abcam ab39180), anti-BACE1 at 1:500 (abcam, ab10716), anti-presenilin-1 at 1:500 (ProSci 4203).

Techniques: Infection, Expressing