pou2f3  (Novus Biologicals)


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    Novus Biologicals pou2f3
    Pou2f3, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson pou2f3
    Pou2f3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology pou2f3
    Pou2f3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology pou2f3
    Pou2f3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson pou2f3
    Pou2f3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore pou2f3
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    pou2f3  (Novus Biologicals)


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    pou2f3  (Danaher Inc)


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    Danaher Inc pou2f3
    Pou2f3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse anti pou2f3
    Classification of SCLC according to IHC of ASCL1, NEUROD1, and <t>POU2F3</t>
    Mouse Anti Pou2f3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Prognostic significance of immunohistochemical classification utilizing biopsy specimens in patients with extensive-disease small-cell lung cancer treated with first-line chemotherapy and immune checkpoint inhibitors"

    Article Title: Prognostic significance of immunohistochemical classification utilizing biopsy specimens in patients with extensive-disease small-cell lung cancer treated with first-line chemotherapy and immune checkpoint inhibitors

    Journal: Journal of Cancer Research and Clinical Oncology

    doi: 10.1007/s00432-024-05652-2

    Classification of SCLC according to IHC of ASCL1, NEUROD1, and POU2F3
    Figure Legend Snippet: Classification of SCLC according to IHC of ASCL1, NEUROD1, and POU2F3

    Techniques Used:

    pou2f3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc pou2f3
    a The POU2AF2 and <t>POU2F3</t> ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.
    Pou2f3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "A SWI/SNF-dependent transcriptional regulation mediated by POU2AF2/C11orf53 at enhancer"

    Article Title: A SWI/SNF-dependent transcriptional regulation mediated by POU2AF2/C11orf53 at enhancer

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46492-5

    a The POU2AF2 and POU2F3 ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.
    Figure Legend Snippet: a The POU2AF2 and POU2F3 ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.

    Techniques Used: ChIP-sequencing, Expressing, Derivative Assay, Software, Activation Assay

    anti pou2f3 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti pou2f3 antibody
    Anti Pou2f3 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    a The POU2AF2 and <t>POU2F3</t> ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.
    Pou2f3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc anti pou2f3 antibody
    a The POU2AF2 and <t>POU2F3</t> ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.
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    Classification of SCLC according to IHC of ASCL1, NEUROD1, and POU2F3

    Journal: Journal of Cancer Research and Clinical Oncology

    Article Title: Prognostic significance of immunohistochemical classification utilizing biopsy specimens in patients with extensive-disease small-cell lung cancer treated with first-line chemotherapy and immune checkpoint inhibitors

    doi: 10.1007/s00432-024-05652-2

    Figure Lengend Snippet: Classification of SCLC according to IHC of ASCL1, NEUROD1, and POU2F3

    Article Snippet: To classify SCLC subtypes, mouse anti-MASH1 (ASCL1) monoclonal antibody (clone 24B72D11.1; eBioscience, San Diego, CA, USA), rabbit anti-NEUROD1 monoclonal antibody (clone EPR20766, Abcam, Cambridge, UK), and mouse anti-POU2F3 (clone 6D1, Santa Cruz Biotechnology, Dallas, TX, USA) were used.

    Techniques:

    a The POU2AF2 and POU2F3 ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.

    Journal: Nature Communications

    Article Title: A SWI/SNF-dependent transcriptional regulation mediated by POU2AF2/C11orf53 at enhancer

    doi: 10.1038/s41467-024-46492-5

    Figure Lengend Snippet: a The POU2AF2 and POU2F3 ChIP-seq was conducted in human SCLC cell line NCI-H526 cells. The overlapping POU2AF2 and POU2F3 peaks ( n = 6987) were divided into three clusters by k-means clustering based on POU2AF2, POU2F3, and histone marks (H3K4me1, H3K27ac, H3K4me3, and H3K27me3). The ChIP-seq signal of these histone marks were further centered on the three clusters. b The scatter plot shows the correlation of gene expression change when POU2F3 or POU2AF2 were depleted by sgRNAs. The significantly altered genes (|log2FC|>1, adj. p < 0.01) by POU2F3/POU2AF2 depletion were highlighted in red (upregulated, n = 774) or blue (downregulated, n = 406). Data are derived from two biological replicates. Genes with Benjamini-Hochburg adjusted p -values less than 0.01 were considered to be differentially expressed in the EdgeR analysis . c Metascape pathway enrichment analysis with up-regulated genes in both POU2F3 and POU2AF2 depleted cells. The -log10(P) value was calculated by Metascape software v3.5 . d The heatmap shows the nearby gene expression change aligned to the corresponding ChIP-seq peak (Fig. 1a) in either POU2AF2 depleted, POU2F3 depleted, or JQ1 treated NCI-H526 cells. Data are derived from two biological replicates. e The Venn diagram shows the overlap between JQ1 target genes and POU2F3/POU2AF2 common target genes. f The enhancer regions marked by H3K4me1 and H3K27me3 ( n = 6538), or H3K4me1 and H3K27ac ( n = 35503) were identified in NCI-H526 cells. The motif analysis shows the most significant motifs enriched in each type of enhancer elements. HOMER screens its library of reliable motifs against the target and background sequences for enrichment, returning motifs enriched with a p-value less than 0.05 . g The track examples show the occupancy of POU2F3 and POU2AF2 at H3K4me1 and H3K27me3 marked enhancer regions, and the activation of nearby gene expression upon the loss of POU2AF2.

    Article Snippet: POU2F3 (#36135), H3K27ac (#8173), H3K4me1 (#5326), H3K4me3 (#9751), H3K27me3 (#9733), Histone H3 (#4499), Cleaved-PARP (#5625), Cleaved Caspase 3 (#9664), SUZ12 (#3737), EZH2 (#5246), BRG1 (#49360), BRM (#11966), ARID1A (#12354), BAF57 (#11956), BAF155 (#11956), BAF60A (#35070), BAF47 (#91735), and PTEN (#9188) antibodies were purchased from Cell Signaling.

    Techniques: ChIP-sequencing, Expressing, Derivative Assay, Software, Activation Assay