Structured Review

Absolute Biotech Inc anti polysialic acid antibody
A Immunohistochemical staining of <t>polysialic</t> acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).
Anti Polysialic Acid Antibody, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti polysialic acid antibody/product/Absolute Biotech Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti polysialic acid antibody - by Bioz Stars, 2023-02
86/100 stars

Images

1) Product Images from "The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer"

Article Title: The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer

Journal: NPJ Breast Cancer

doi: 10.1038/s41523-022-00442-w

A Immunohistochemical staining of polysialic acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).
Figure Legend Snippet: A Immunohistochemical staining of polysialic acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).

Techniques Used: Immunohistochemical staining, Staining, Expressing

A Overall survival of individuals based on ST8Sia4 expression in tumor cells. Patients were categorized as either ST8Sia4 high (>1.25 RNA punctae/cell) or low ST8Sia4 (≤1.25 RNA puncate/cell). B Animated diagram depicting the prognostic significance of ST8Sia4 expression in tumor cells. C Overall survival of individuals based on polysialic acid levels in tumor cells. Patients were categorized as high expression ( H -Score > 0.66) or moderate/low ( H -Score ≤ 0.66). High ST8Sia4 and polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test. D Overall survival of individuals based on infiltrating stromal cell ST8Sia4 expression in TIL-positive tumor cores. Patients were categorized as either ST8Sia4 high (>0.4 RNA punctae/cell) or low ST8Sia4 (≤0.4 RNA punctae/cell). High ST8Sia4 cut-point value was set at the median ± SEM of all values. E Animated diagram depicting the prognostic significance of ST8Sia4 expression in infiltrating stromal cells. F Overall survival of individuals based on polysialic acid levels on TIL. Patients were categorized as high expression ( H -Score > 0.55) or moderate/low ( H -Score ≤ 0.55). TIL Negative (tumor cores absent for TIL). High polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test.
Figure Legend Snippet: A Overall survival of individuals based on ST8Sia4 expression in tumor cells. Patients were categorized as either ST8Sia4 high (>1.25 RNA punctae/cell) or low ST8Sia4 (≤1.25 RNA puncate/cell). B Animated diagram depicting the prognostic significance of ST8Sia4 expression in tumor cells. C Overall survival of individuals based on polysialic acid levels in tumor cells. Patients were categorized as high expression ( H -Score > 0.66) or moderate/low ( H -Score ≤ 0.66). High ST8Sia4 and polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test. D Overall survival of individuals based on infiltrating stromal cell ST8Sia4 expression in TIL-positive tumor cores. Patients were categorized as either ST8Sia4 high (>0.4 RNA punctae/cell) or low ST8Sia4 (≤0.4 RNA punctae/cell). High ST8Sia4 cut-point value was set at the median ± SEM of all values. E Animated diagram depicting the prognostic significance of ST8Sia4 expression in infiltrating stromal cells. F Overall survival of individuals based on polysialic acid levels on TIL. Patients were categorized as high expression ( H -Score > 0.55) or moderate/low ( H -Score ≤ 0.55). TIL Negative (tumor cores absent for TIL). High polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test.

Techniques Used: Expressing


Structured Review

Absolute Biotech anti polysialic acid 735 antibody
Anti Polysialic Acid 735 Antibody, supplied by Absolute Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti polysialic acid 735 antibody/product/Absolute Biotech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti polysialic acid 735 antibody - by Bioz Stars, 2023-02
86/100 stars

Images


Structured Review

Absolute Biotech Inc anti polysialic acid 735 antibody
Anti Polysialic Acid 735 Antibody, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti polysialic acid 735 antibody/product/Absolute Biotech Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti polysialic acid 735 antibody - by Bioz Stars, 2023-02
86/100 stars

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    Absolute Biotech Inc anti polysialic acid antibody
    A Immunohistochemical staining of <t>polysialic</t> acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).
    Anti Polysialic Acid Antibody, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti polysialic acid antibody/product/Absolute Biotech Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti polysialic acid antibody - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Absolute Biotech anti polysialic acid 735 antibody
    A Immunohistochemical staining of <t>polysialic</t> acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).
    Anti Polysialic Acid 735 Antibody, supplied by Absolute Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti polysialic acid 735 antibody/product/Absolute Biotech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti polysialic acid 735 antibody - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    86
    Absolute Biotech Inc anti polysialic acid 735 antibody
    A Immunohistochemical staining of <t>polysialic</t> acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).
    Anti Polysialic Acid 735 Antibody, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti polysialic acid 735 antibody/product/Absolute Biotech Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti polysialic acid 735 antibody - by Bioz Stars, 2023-02
    86/100 stars
      Buy from Supplier

    Image Search Results


    A Immunohistochemical staining of polysialic acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).

    Journal: NPJ Breast Cancer

    Article Title: The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer

    doi: 10.1038/s41523-022-00442-w

    Figure Lengend Snippet: A Immunohistochemical staining of polysialic acid in normal tissue and breast cancer tissue. Representative images of normal breast tissue and tumors showing within each tissue or tumor category of low expression (top row) and high expression (bottom row). Cores are shown alongside higher magnification insets from the same core. B Analysis of polysialic acid expression ( H -Score) in normal breast tissue and tumor cells categorized into molecular subtype. Luminal A ( n = 60), Luminal B ( n = 9), HER2+ ( n = 12), and triple-negative breast cancer (TNBC) ( n = 42). Bars indicate medians and ±SEM, **** P < 0.0001, Kruskal–Wallis test. Identification of polysialic positive tumor infiltrating lymphocytes in the tumor microenvironment by immunohistochemical staining of polysialic acid ( C ) and CD3 ( D ). Representative images are shown alongside higher magnification insets from the same core. Arrows point to lymphocytes positive for polySia ( C ) and regions of lymphocytes positive for both polySia and CD3 ( D ). E CD3 positive tumor-infiltrating lymphocytes express polySia. Breast tumor tissue was immunostained for CD3 (red) and polySia (green), followed by a nuclei stain (blue). Bottom row represents zooms from corresponding image in the top row. Arrows point to CD3-positive cells (red) which are also positive for polySia (green), and can be visualized as yellow in the overlay. Scale bar = 100 µm. F and G Analysis of polysialic acid expression ( H -Score) on tumor-infiltrating lymphocytes categorized by molecular subtype (Luminal A, n = 36; Luminal B, n = 6; HER2+, n = 8; TNBC, n = 31) ( F ) or estrogen receptor expression (strong, n = 21; weak, n = 51; moderate:n = 13) ( G ). Representative images of polySia TIL expression in ER positive ( H ; arrows points to lymphocytes with low/no polySia) and ER negative ( I ; arrows point to yellow/polySia positive lymphocytes). Bars indicate medians and ±SEM, p values shown from Tukey’s test. Scale bar = 200 µm (cores) and 50 or 20 µm (insets).

    Article Snippet: Anti-polysialic acid antibody [Clone 735] was purchased through Absolute Antibody Ltd. (Cleveland, UK: Cat. No. Ab00240-2.0; RRID:AB_2619682).

    Techniques: Immunohistochemical staining, Staining, Expressing

    A Overall survival of individuals based on ST8Sia4 expression in tumor cells. Patients were categorized as either ST8Sia4 high (>1.25 RNA punctae/cell) or low ST8Sia4 (≤1.25 RNA puncate/cell). B Animated diagram depicting the prognostic significance of ST8Sia4 expression in tumor cells. C Overall survival of individuals based on polysialic acid levels in tumor cells. Patients were categorized as high expression ( H -Score > 0.66) or moderate/low ( H -Score ≤ 0.66). High ST8Sia4 and polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test. D Overall survival of individuals based on infiltrating stromal cell ST8Sia4 expression in TIL-positive tumor cores. Patients were categorized as either ST8Sia4 high (>0.4 RNA punctae/cell) or low ST8Sia4 (≤0.4 RNA punctae/cell). High ST8Sia4 cut-point value was set at the median ± SEM of all values. E Animated diagram depicting the prognostic significance of ST8Sia4 expression in infiltrating stromal cells. F Overall survival of individuals based on polysialic acid levels on TIL. Patients were categorized as high expression ( H -Score > 0.55) or moderate/low ( H -Score ≤ 0.55). TIL Negative (tumor cores absent for TIL). High polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test.

    Journal: NPJ Breast Cancer

    Article Title: The different prognostic significance of polysialic acid and CD56 expression in tumor cells and lymphocytes identified in breast cancer

    doi: 10.1038/s41523-022-00442-w

    Figure Lengend Snippet: A Overall survival of individuals based on ST8Sia4 expression in tumor cells. Patients were categorized as either ST8Sia4 high (>1.25 RNA punctae/cell) or low ST8Sia4 (≤1.25 RNA puncate/cell). B Animated diagram depicting the prognostic significance of ST8Sia4 expression in tumor cells. C Overall survival of individuals based on polysialic acid levels in tumor cells. Patients were categorized as high expression ( H -Score > 0.66) or moderate/low ( H -Score ≤ 0.66). High ST8Sia4 and polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test. D Overall survival of individuals based on infiltrating stromal cell ST8Sia4 expression in TIL-positive tumor cores. Patients were categorized as either ST8Sia4 high (>0.4 RNA punctae/cell) or low ST8Sia4 (≤0.4 RNA punctae/cell). High ST8Sia4 cut-point value was set at the median ± SEM of all values. E Animated diagram depicting the prognostic significance of ST8Sia4 expression in infiltrating stromal cells. F Overall survival of individuals based on polysialic acid levels on TIL. Patients were categorized as high expression ( H -Score > 0.55) or moderate/low ( H -Score ≤ 0.55). TIL Negative (tumor cores absent for TIL). High polySia cut-point value was set at the 75% Percentile ±SEM of all values. Overall Survival curve comparison: Log-rank (Mantel–Cox) test.

    Article Snippet: Anti-polysialic acid antibody [Clone 735] was purchased through Absolute Antibody Ltd. (Cleveland, UK: Cat. No. Ab00240-2.0; RRID:AB_2619682).

    Techniques: Expressing