phosphorylated p akt  (Boster Bio)


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    Boster Bio phosphorylated p akt
    PC-3-derived exosomes block the <t>AKT</t> and mTOR pathways by miR-148a downregulation. (A) Western blotting of <t>p-AKT,</t> AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.
    Phosphorylated P Akt, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated p akt/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated p akt - by Bioz Stars, 2023-09
    86/100 stars

    Images

    1) Product Images from "Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway"

    Article Title: Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2021.10739

    PC-3-derived exosomes block the AKT and mTOR pathways by miR-148a downregulation. (A) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.
    Figure Legend Snippet: PC-3-derived exosomes block the AKT and mTOR pathways by miR-148a downregulation. (A) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.

    Techniques Used: Derivative Assay, Blocking Assay, Western Blot, Transfection, Negative Control

    anti phosphorylated p akt  (Boster Bio)


    Bioz Verified Symbol Boster Bio is a verified supplier
    Bioz Manufacturer Symbol Boster Bio manufactures this product  
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    • 86
      Buy from Supplier

    Structured Review

    Boster Bio anti phosphorylated p akt
    Anti Phosphorylated P Akt, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated p akt/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphorylated p akt - by Bioz Stars, 2023-09
    86/100 stars

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    Similar Products

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    • phosphorylated p akt  (Boster Bio)
    86
    Boster Bio phosphorylated p akt
    PC-3-derived exosomes block the <t>AKT</t> and mTOR pathways by miR-148a downregulation. (A) Western blotting of <t>p-AKT,</t> AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.
    Phosphorylated P Akt, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated p akt/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated p akt - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier
    anti phosphorylated p akt  (Boster Bio)
    86
    Boster Bio anti phosphorylated p akt
    PC-3-derived exosomes block the <t>AKT</t> and mTOR pathways by miR-148a downregulation. (A) Western blotting of <t>p-AKT,</t> AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.
    Anti Phosphorylated P Akt, supplied by Boster Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphorylated p akt/product/Boster Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphorylated p akt - by Bioz Stars, 2023-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    PC-3-derived exosomes block the AKT and mTOR pathways by miR-148a downregulation. (A) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.

    Journal: Experimental and Therapeutic Medicine

    Article Title: Exosomes derived from PC-3 cells suppress osteoclast differentiation by downregulating miR-148a and blocking the PI3K/AKT/mTOR pathway

    doi: 10.3892/etm.2021.10739

    Figure Lengend Snippet: PC-3-derived exosomes block the AKT and mTOR pathways by miR-148a downregulation. (A) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts treated with 0, 10, 30 or 50 ng exosomes/1,000 cells. (B) Western blotting of p-AKT, AKT, p-mTOR and mTOR in osteoclasts transfected with miR-148a mimic, inhibitor, mimic NC or inhibitor NC after induction with M-CSF and RANKL. (C) Semi-quantification of western blot analysis of p-AKT/AKT and p-mTOR/mTOR. ** P<0.01, *** P<0.001, **** P<0.0001 0 vs. 10; ### P<0.001, #### P<0.0001 10 vs. 30; &&&& P<0.0001 30 vs. 50 ng. p-, phosphorylation; miR, microRNA; NC, negative control; M-CSF, macrophage colony-stimulating factor; RANKL, receptor activator of nuclear factor κB ligand.

    Article Snippet: The membranes were blocked in 5% nonfat dry milk diluted in tris-buffered saline containing 0.1% Tween 20 (TBST) at room temperature for 1 h. Afterwards, the membranes were incubated with primary rabbit monoclonal antibodies against the following antigens at 4˚C overnight: MafB (1:1,000; Zen-bio; cat. no. 862796), MMP-9 (1:1,000; Abcam; #EP1254), Iβ3 (1:1,000; Cell Signaling Technology, Inc.), AKT, phosphorylated (p)-AKT, mTOR, p-mTOR and GAPDH (all 1:2,000; all Wuhan Boster Biological Technology, Ltd.).

    Techniques: Derivative Assay, Blocking Assay, Western Blot, Transfection, Negative Control