phosphor ampk p ampk (Cell Signaling Technology Inc)
Cell Signaling Technology Inc manufactures this product
Structured Review
Phosphor Ampk P Ampk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphor ampk p ampk/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
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1) Product Images from "Decreased AMPK/SIRT1/PDK4 induced by androgen excess inhibits human endometrial stromal cell decidualization in PCOS"
Article Title: Decreased AMPK/SIRT1/PDK4 induced by androgen excess inhibits human endometrial stromal cell decidualization in PCOS
Journal: Cellular and Molecular Life Sciences: CMLS
doi: 10.1007/s00018-024-05362-5
Figure Legend Snippet: Effects of AMPK on decidualization. A and B HESCs were pretreated with 8-Br-cAMP and MPA for three days, followed by treatment with various concentrations of testosterone (0.001–100 μM) for an additional two days. AMPK ( A ) and SIRT1 ( B ) mRNA levels were further measured using real-time PCR. Statistical significance is denoted by * p < 0.05. C HESCs were treated with 8-Br-cAMP and MPA for 1–5 days, and AMPK and SIRT1 mRNA levels were further measured using real-time PCR. D -I HESCs were pretreated with 8-Br-cAMP and MPA for three days, followed by treatment with Dorsomorphin (5 μM) or A-769662 (20 μM) alone or combining Dorsomorphin/A-769662 with testosterone (100 μM) for an additional two days. IGFBP1 ( D ) and PRL ( E ) mRNA levels were further measured using real-time PCR. IGFBP1 protein ( F ) that was released into the medium was measured using ELISA. PDK4 ( G ) and SIRT1 ( H ) mRNA levels were further measured using real-time PCR. Statistical significance is denoted by * p < 0.05, ** p < 0.01. I Proteins of p-AMPK, SIRT1, and PDK4 were measured using Western blot analysis. Quantitative analysis of p-AMPK (J), SIRT1 (K), and PDK4 (L) protein expression levels were showed in histogram. a represents the treated cells vs. non-decidualized cells. b represents the treated cells vs. decidualized cells without other treatments. Statistical significance is denoted by * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001
Techniques Used: Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing
Figure Legend Snippet: Androgen excess regulates decidualization via the AR/AMPK/SIRT1/PDK4 pathway. PDK4 levels were induced during decidualization. PDK4 expression decreased in the endometrium of PCOS patients. Androgen excess regulates decidualization via the AR/AMPK/SIRT1/PDK4 pathway
Techniques Used: Expressing