Journal: Scientific Reports

Article Title: LINGO-1 antibody ameliorates myelin impairment and spatial memory deficits in experimental autoimmune encephalomyelitis mice

doi: 10.1038/srep14235

Figure Lengend Snippet: Compared to the control group, we also did not observe any obvious differences in the levels of Ser473-phosphorylated AKT in the EAE mice. However, compared to the EAE group, treatment with the LINGO-1 antibody clearly increased the levels of the Ser473-phosphorylated AKT. ( c ) Similar results were observed for p-mTOR. The level of p-mTOR was increased in the LINGO-1 antibody treated group. ( b,d ) No obvious differences were observed in the total levels of AKT and m-TOR among the three groups. *Denotes statistical significance compared with controls (P < 0.05). # Denotes statistical significance compared with EAE mice without treatment (P < 0.05).

Article Snippet: The membranes were probed overnight at 4 °C with the following antibodies and dilutions: anti-MBP (anti-rat monoclonal; 1:2000; Abcam), anti-dynein (anti-mouse monoclonal; 1:800; Abcam), anti-KLC (anti-rabbit polyclonal; 1:500; Santa), anti-NF200 (anti-rabbit polyclonal; 1:2000; Sigma), anti-phospho-Akt (Ser473) (anti-rabbit monoclonal; 1:2000; Cell Signaling Technology), anti-Akt (anti-rabbit monoclonal; 1:2000; Cell Signaling Technology), anti-phospho-mTOR (Ser 2448) (anti-rabbit polyclonal antibody; 1:500; Bioworld Technology), anti-mTOR (anti-rabbit polyclonal antibody; 1:500; Bioworld Technology), and anti-α-tubulin (anti-rabbit monoclonal; 1:2000; KeyGEN Biotech).

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