Journal: Experimental and Therapeutic Medicine

Article Title: Protective effect of leukemia inhibitory factor on the retinal injury induced by acute ocular hypertension in rats

doi: 10.3892/etm.2022.11717

Figure Lengend Snippet: Effects of LIF on the expression and activation of AKT/mTOR/p70S6k signaling pathway components after AOH. (A) Representative western blotting images, and semi-quantitative analysis of the (B) p-AKT/AKT, (C) p-mTOR/mTOR and (D) p-p70S6K/p70S6K ratios. * P<0.05, # P<0.001, n=3/group. AOH, acute ocular hypertension; LIF, leukemia inhibitory factor; p-, phosphorylated; p70S6K, ribosomal protein S6 kinase.

Article Snippet: The antibodies of cleaved-caspase-3 (cat. no. 9579), PARP (cat. no. 9542), AKT (cat. no. 9272), p-AKT (cat. no. 31957), mTOR (cat. no. 2972), p-mTOR (cat. no. 5536), p70S6K (cat. no. 9202) and p-p70S6K (cat. no. 9208) were purchased from Cell Signaling Technology, Inc., whilst the antibodies of STAT3 (cat. no. APR13562G) and p-STAT3 (cat. no. APR11162G) were bought from Santa Cruz Biotechnology, Inc. After washing with TBS + 1% Tween 20 three times, the membranes were incubated with an HRP-conjugated goat anti-rabbit IgG secondary antibody (1:10,000; cat. no. 172-1050; Bio-Rad Laboratories, Inc.) for 2 h at room temperature.

Techniques: Expressing, Activation Assay, Western Blot

Journal: iScience

Article Title: TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

doi: 10.1016/j.isci.2023.106543

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-phospho Ser371-p70S6K , CellSignaling , Cat #9208.

Techniques: Purification, shRNA, Transduction, Recombinant, Reporter Assay, Lysis, Plasmid Preparation, Software

Journal: PLoS ONE

Article Title: Vascular Endothelial Growth Factor Receptor-3 Directly Interacts with Phosphatidylinositol 3-Kinase to Regulate Lymphangiogenesis

doi: 10.1371/journal.pone.0039558

Figure Lengend Snippet: Western blotting analysis of phosphorylated P70S6K ( A, top left ) and eNOS (S1177) ( B, top left ) in prostatic LECs following 15 minute stimulation with ligand: VEGF-C (100 ng/ml), VEGF-A (100 ng/ml), VEGF-C156S (250 ng/ml), VEGF-D (250 ng/ml) and VEGF-E (100 ng/ml). Time- and concentration-dependent phosphorylation of P70S6K ( A, top right ), eNOS (S1177) ( B, top right ), and PLCγ1 (Tyr783) ( C, top left ) in LECs in response to VEGF-C. The effect of inhibition of VEGFR-3 (hF4-3C5), VEGFR-2 (IMC-1121b) or VEGFR-1 (IMC-18F1) on phosphorylation of P70S6K ( A, bottom left ), eNOS (S1177) ( B, bottom left ); and VEGFR-3 (hF4-3C5) on PLCγ1 (Tyr783) ( C, bottom right ) on LEC response to VEGF-C (100 ng/ml). The effect of AS252424, LY294002, PD98059, and U-73122 on phosphorylation of P70S6K ( A, top right ), eNOS (S1177) ( B, bottom right ), and PLCγ1 (Tyr783) ( C, bottom right ) on LECs in response to VEGF-C. The effect of VEGF-C on phosphorylation of PLCγ2 (Tyr759, Tyr1217) in LECs ( C, bottom left ). Control serum-free vehicle treated LEC lysate is indicated by ‘0′ in all blots. n = 3. Densitometry analysis is shown under each blot in italics; where integrated intensity of phosphorylated molecules was firstly compared to that of the total target protein for each sample, and then expressed as fold change in integrated density compared to either serum-free (A, top left and right panels ; B, top left and right panels ; C, top and bottom left panels ) or VEGF-C treated control samples (A, bottom left and right panels ; B, bottom left and right panels ; C, bottom and bottom left panels ).

Article Snippet: Antibodies against total P70S6K (Clone: 49D7, Cat. no. 9208), mTOR (Cat. no. 2972), eNOS (Clone: 49G3, Cat. no. 9586), PLCγ1 (Clone: D9H10, Cat. no. 5690), PLCγ2 (Cat. no. 3872), Erk1/2 (Clone: 137F5; Cat. no. 4695), and Akt (Cat. no. 9272) were purchased from Cell Signaling Technology (MA).

Techniques: Western Blot, Concentration Assay, Inhibition