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Santa Cruz Biotechnology anti phospho raf 1
Knockdown (KD) of Plk1 attenuates the PDGF-induced phosphorylation of MEK1/2 and ERK1/2, but not <t>Raf-1</t> phosphorylation. a Representative immunoblots illustrating the effects of Plk1shRNA on Plk1 expression. Blots of HASM cells infected with lentiviruses encoding control shRNA or Plk1 shRNA were probed with antibodies against Plk1 and GAPDH. Duplicated samples of each treatment are shown. Ratios of Plk1/GAPDH protein in cells producing Plk1 shRNA were normalized to ratios obtained from cells producing control shRNA. Values are mean ± SE ( n = 4). *Significantly lower Plk1/GAPDH ratios in cells producing Plk1 shRNA compared with cells producing control shRNA ( P
Anti Phospho Raf 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho raf 1/product/Santa Cruz Biotechnology
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti phospho raf 1 - by Bioz Stars, 2020-07
88/100 stars

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1) Product Images from "Plk1 regulates MEK1/2 and proliferation in airway smooth muscle cells"

Article Title: Plk1 regulates MEK1/2 and proliferation in airway smooth muscle cells

Journal: Respiratory Research

doi: 10.1186/s12931-015-0257-8

Knockdown (KD) of Plk1 attenuates the PDGF-induced phosphorylation of MEK1/2 and ERK1/2, but not Raf-1 phosphorylation. a Representative immunoblots illustrating the effects of Plk1shRNA on Plk1 expression. Blots of HASM cells infected with lentiviruses encoding control shRNA or Plk1 shRNA were probed with antibodies against Plk1 and GAPDH. Duplicated samples of each treatment are shown. Ratios of Plk1/GAPDH protein in cells producing Plk1 shRNA were normalized to ratios obtained from cells producing control shRNA. Values are mean ± SE ( n = 4). *Significantly lower Plk1/GAPDH ratios in cells producing Plk1 shRNA compared with cells producing control shRNA ( P
Figure Legend Snippet: Knockdown (KD) of Plk1 attenuates the PDGF-induced phosphorylation of MEK1/2 and ERK1/2, but not Raf-1 phosphorylation. a Representative immunoblots illustrating the effects of Plk1shRNA on Plk1 expression. Blots of HASM cells infected with lentiviruses encoding control shRNA or Plk1 shRNA were probed with antibodies against Plk1 and GAPDH. Duplicated samples of each treatment are shown. Ratios of Plk1/GAPDH protein in cells producing Plk1 shRNA were normalized to ratios obtained from cells producing control shRNA. Values are mean ± SE ( n = 4). *Significantly lower Plk1/GAPDH ratios in cells producing Plk1 shRNA compared with cells producing control shRNA ( P

Techniques Used: Western Blot, Expressing, Infection, shRNA

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    Santa Cruz Biotechnology anti phospho raf 1 antibodies
    Sustained Ras-Raf complex formation in RA T cells. T cells were stimulated, fixed, and stained with antibodies for signaling molecules. Representative cells stained for <t>Raf-1</t> and K-Ras (A) or N-Ras (B) are shown. In C, D, F, and G, fluorescence intensities in membrane-gated regions were quantified; results are expressed as fold-difference compared to unstimulated control cells and are shown as box plots of 75 cells from 5 patients (shaded boxes) and 5 controls (open boxes). In Figures 5E and H, co-localization of green and red fluorescence (indicated by yellow color in A, B) was examined. Fluorescence for each pixel was correlated, and the data are shown as the correlation coefficients. (I) T cells were stimulated by CD3 cross-linking; cell lysates were obtained at indicated time points and analyzed by Western blotting for p-c-Raf. Results are representative of 3 experiments with 9 RA patients and 9 controls. * p
    Anti Phospho Raf 1 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho raf 1 antibodies/product/Santa Cruz Biotechnology
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    anti phospho raf 1 antibodies - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology rabbit anti human phospho raf
    Sustained Ras-Raf complex formation in RA T cells. T cells were stimulated, fixed, and stained with antibodies for signaling molecules. Representative cells stained for <t>Raf-1</t> and K-Ras (A) or N-Ras (B) are shown. In C, D, F, and G, fluorescence intensities in membrane-gated regions were quantified; results are expressed as fold-difference compared to unstimulated control cells and are shown as box plots of 75 cells from 5 patients (shaded boxes) and 5 controls (open boxes). In Figures 5E and H, co-localization of green and red fluorescence (indicated by yellow color in A, B) was examined. Fluorescence for each pixel was correlated, and the data are shown as the correlation coefficients. (I) T cells were stimulated by CD3 cross-linking; cell lysates were obtained at indicated time points and analyzed by Western blotting for p-c-Raf. Results are representative of 3 experiments with 9 RA patients and 9 controls. * p
    Rabbit Anti Human Phospho Raf, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti human phospho raf/product/Santa Cruz Biotechnology
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti human phospho raf - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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    Sustained Ras-Raf complex formation in RA T cells. T cells were stimulated, fixed, and stained with antibodies for signaling molecules. Representative cells stained for Raf-1 and K-Ras (A) or N-Ras (B) are shown. In C, D, F, and G, fluorescence intensities in membrane-gated regions were quantified; results are expressed as fold-difference compared to unstimulated control cells and are shown as box plots of 75 cells from 5 patients (shaded boxes) and 5 controls (open boxes). In Figures 5E and H, co-localization of green and red fluorescence (indicated by yellow color in A, B) was examined. Fluorescence for each pixel was correlated, and the data are shown as the correlation coefficients. (I) T cells were stimulated by CD3 cross-linking; cell lysates were obtained at indicated time points and analyzed by Western blotting for p-c-Raf. Results are representative of 3 experiments with 9 RA patients and 9 controls. * p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: ERK-dependent T-cell receptor threshold calibration in rheumatoid arthritis

    doi: 10.4049/jimmunol.0901784

    Figure Lengend Snippet: Sustained Ras-Raf complex formation in RA T cells. T cells were stimulated, fixed, and stained with antibodies for signaling molecules. Representative cells stained for Raf-1 and K-Ras (A) or N-Ras (B) are shown. In C, D, F, and G, fluorescence intensities in membrane-gated regions were quantified; results are expressed as fold-difference compared to unstimulated control cells and are shown as box plots of 75 cells from 5 patients (shaded boxes) and 5 controls (open boxes). In Figures 5E and H, co-localization of green and red fluorescence (indicated by yellow color in A, B) was examined. Fluorescence for each pixel was correlated, and the data are shown as the correlation coefficients. (I) T cells were stimulated by CD3 cross-linking; cell lysates were obtained at indicated time points and analyzed by Western blotting for p-c-Raf. Results are representative of 3 experiments with 9 RA patients and 9 controls. * p

    Article Snippet: 30 μg of total protein were resolved on SDS-PAGE under reducing conditions, transferred to PVDF membranes, and incubated at 4°C with anti-phospho-ERK1/2 or anti-phospho-Raf-1 antibodies followed by washing and incubation with HRP-conjugated secondary antibodies (Santa Cruz Biotechnology).

    Techniques: Staining, Fluorescence, Western Blot

    Effect of eugenol on MEK1/2 and Raf phosphorylation in PMA-stimulated neutrophils. Cells were incubated for 30 min at 37 °C either without or with increased concentrations of EUG (0, 2.5, 5, 10, 20 µg/mL), followed by PMA-stimulation for 8 min. Cell lysates were analyzed by SDS-PAGE and western blot using anti-phospho-MEK 1/2, anti-phospho-Raf and anti-β-actine antibodies. ( A ) Western blots from different experiments were scanned and the intensity of bands was expressed relative to β-actine amount. The cumulated data of phospho-MEK 1/2 (B) and phospho-Raf (C) is shown in the histogram as percentage to control (PMA alone 100%). Data are expressed as mean ± SEM of three or more separate experiments. p

    Journal: Scientific Reports

    Article Title: Eugenol prevents fMLF-induced superoxide anion production in human neutrophils by inhibiting ERK1/2 signaling pathway and p47phox phosphorylation

    doi: 10.1038/s41598-019-55043-8

    Figure Lengend Snippet: Effect of eugenol on MEK1/2 and Raf phosphorylation in PMA-stimulated neutrophils. Cells were incubated for 30 min at 37 °C either without or with increased concentrations of EUG (0, 2.5, 5, 10, 20 µg/mL), followed by PMA-stimulation for 8 min. Cell lysates were analyzed by SDS-PAGE and western blot using anti-phospho-MEK 1/2, anti-phospho-Raf and anti-β-actine antibodies. ( A ) Western blots from different experiments were scanned and the intensity of bands was expressed relative to β-actine amount. The cumulated data of phospho-MEK 1/2 (B) and phospho-Raf (C) is shown in the histogram as percentage to control (PMA alone 100%). Data are expressed as mean ± SEM of three or more separate experiments. p

    Article Snippet: The membranes were incubated overnight at 4 °C in solution containing specific relevant primary antibodies; anti-phospho-S328-p47phox (1:2500), anti-phospho-S345-p47phox (1:10000), anti-phospho-Raf (1:1000), anti-phospho-MEK1/2 (1:1000), anti-phospho-ERK1/2 (1/2000), anti-phospho-p38 (1:2000) and p47phox (1:5000), following by incubation in horseradish peroxidase conjugated secondary antibodies (Santa Cruz, Heidelberg, Germany).

    Techniques: Incubation, SDS Page, Western Blot

    Effect of eugenol on MEK1/2 and Raf phosphorylation in fMLF-stimulated neutrophils. Cells were incubated for 30 min at 37 °C either without or with increased concentrations of EUG (0, 2.5, 5, 10, 20 µg/mL), followed by fMLF-stimulation for 15 s. Cell lysates were analyzed by SDS-PAGE and western blot using anti-phospho-MEK 1/2, anti-phospho-Raf and anti-β-actine antibodies. ( A ) Western blots from different experiments were scanned and the intensity of bands was expressed relative to β-actine amount. The cumulated data of phospho-MEK 1/2 (B) and phospho-Raf ( C ) is shown in the histogram as percentage to control (FMLF alone 100%). Data are expressed as mean ± SEM of three or more separate experiments. p

    Journal: Scientific Reports

    Article Title: Eugenol prevents fMLF-induced superoxide anion production in human neutrophils by inhibiting ERK1/2 signaling pathway and p47phox phosphorylation

    doi: 10.1038/s41598-019-55043-8

    Figure Lengend Snippet: Effect of eugenol on MEK1/2 and Raf phosphorylation in fMLF-stimulated neutrophils. Cells were incubated for 30 min at 37 °C either without or with increased concentrations of EUG (0, 2.5, 5, 10, 20 µg/mL), followed by fMLF-stimulation for 15 s. Cell lysates were analyzed by SDS-PAGE and western blot using anti-phospho-MEK 1/2, anti-phospho-Raf and anti-β-actine antibodies. ( A ) Western blots from different experiments were scanned and the intensity of bands was expressed relative to β-actine amount. The cumulated data of phospho-MEK 1/2 (B) and phospho-Raf ( C ) is shown in the histogram as percentage to control (FMLF alone 100%). Data are expressed as mean ± SEM of three or more separate experiments. p

    Article Snippet: The membranes were incubated overnight at 4 °C in solution containing specific relevant primary antibodies; anti-phospho-S328-p47phox (1:2500), anti-phospho-S345-p47phox (1:10000), anti-phospho-Raf (1:1000), anti-phospho-MEK1/2 (1:1000), anti-phospho-ERK1/2 (1/2000), anti-phospho-p38 (1:2000) and p47phox (1:5000), following by incubation in horseradish peroxidase conjugated secondary antibodies (Santa Cruz, Heidelberg, Germany).

    Techniques: Incubation, SDS Page, Western Blot

    Knockdown (KD) of Plk1 attenuates the PDGF-induced phosphorylation of MEK1/2 and ERK1/2, but not Raf-1 phosphorylation. a Representative immunoblots illustrating the effects of Plk1shRNA on Plk1 expression. Blots of HASM cells infected with lentiviruses encoding control shRNA or Plk1 shRNA were probed with antibodies against Plk1 and GAPDH. Duplicated samples of each treatment are shown. Ratios of Plk1/GAPDH protein in cells producing Plk1 shRNA were normalized to ratios obtained from cells producing control shRNA. Values are mean ± SE ( n = 4). *Significantly lower Plk1/GAPDH ratios in cells producing Plk1 shRNA compared with cells producing control shRNA ( P

    Journal: Respiratory Research

    Article Title: Plk1 regulates MEK1/2 and proliferation in airway smooth muscle cells

    doi: 10.1186/s12931-015-0257-8

    Figure Lengend Snippet: Knockdown (KD) of Plk1 attenuates the PDGF-induced phosphorylation of MEK1/2 and ERK1/2, but not Raf-1 phosphorylation. a Representative immunoblots illustrating the effects of Plk1shRNA on Plk1 expression. Blots of HASM cells infected with lentiviruses encoding control shRNA or Plk1 shRNA were probed with antibodies against Plk1 and GAPDH. Duplicated samples of each treatment are shown. Ratios of Plk1/GAPDH protein in cells producing Plk1 shRNA were normalized to ratios obtained from cells producing control shRNA. Values are mean ± SE ( n = 4). *Significantly lower Plk1/GAPDH ratios in cells producing Plk1 shRNA compared with cells producing control shRNA ( P

    Article Snippet: Antibodies used were anti-phospho-Plk1 (Abcam), anti-Plk1 (EMD Millipore), anti-phospho-MEK1/2 (Santa Cruz Biotech.), anti-MEK1/2 (Santa Cruz Biotech.), anti-phospho-ERK1/2 (Cell signaling), anti-ERK1/2 (Cell signaling), anti-phospho-Raf-1 (Santa Cruz Biotech.), anti-Raf-1 (Santa Cruz Biotech.) and anti-GAPDH (Ambion).

    Techniques: Western Blot, Expressing, Infection, shRNA