Journal: International Journal of Nanomedicine
Article Title: LBP-CD155 Liposome Nanovaccine Efficiently Resist Colorectal Cancer and Enhance ICB Therapy
doi: 10.2147/IJN.S492734
Figure Lengend Snippet: Nanovaccine-induced BMDCs maturation and pathways induced by nanovaccine. After different nanoparticles were coincubated with BMDCs for 24h, the expression of the costimulatory molecules CD80, CD86, MHC I and MHC II on BMDCs was detected by Flow Cytometry ( A ). The effect of different nanoparticles on cytokine IL-6 and IL-12p40 secretion in BMDCs was detected by ELISA Kits (B) . Effects of blocking MGL on the expression of MGL and TLR4 pathways in DCs maturation. Western blotting (C) and TaqMan qPCR (D) analyze the TLR4, MGL, P38, p-P38, JNK,p-JNK, Erk1/2, p-Erk1/2, NF-κB and p-NF-κB protein levels after transfection immature DCs with 100 nM/L siRNA MGL. The cytokines IL-6 and IL-12p40 (E) were measured by ELISA Kits after transfection of immature DCs with 100 nM/L siRNA MGL. LBP-L vaccination increased cDC1 cells maturation. The TaqMan qPCR analyzes the expression levels of Irf4 and Batf3 (F) after transfection immature DCs with 100 nM/L siRNA MGL. n=3 “ns” not significant, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Article Snippet: After blocking with 5% fat-free milk for 2 h, the PVDF membranes were incubated with the following primary antibodies: Anti-MGL antibody (1:1,000) (cat. no. GR3397231-1; Abcam, UK), anti-TLR4 antibody (1:1,000) (cat. no. sc-293072GR3397231-1; Santa Cruz, USA), anti-Erk1/2 antibody (1:1,000) (cat. no. ab4695S; Cell Signaling Technology, USA), anti-phospho-Erk1/2 antibody (1:1,000) (cat. no. 4376S; Cell Signaling Technology, USA), anti-P38 antibody (1:1,000) (cat. no. 8690S; Cell Signaling Technology, USA), anti-phospho-P38 antibody (1:1,000) (cat. no. 4092S; Cell Signaling Technology, USA), anti-JNK antibody (1:1,000) (cat. no. 9252S; Cell Signaling Technology, USA), anti-phospho-JNK antibody (1:1,000) (cat.no.4668S; Cell Signaling Technology, USA), anti-NF-κB antibody (1:1,000) (cat. no. 8242S; Cell Signaling Technology, USA), and anti-phospho NF-κB antibody (1:1,000) (cat. no. 3033S; Cell Signaling Technology, USA).
Techniques: Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Blocking Assay, Western Blot, Transfection
Cell Signaling Technology Inc manufactures this product 
