anti phospho irf7 ser437 438  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho irf7 ser437 438
    a , Serum IL-12p70, IP-10, IL-6 and IFNγ of the indicated mice ( n = 5 for IL-12p70 and IP-10; n = 4 for IL-6 and IFNγ). IP-10 mice were aged 8 months and IL-12p70, IL-6 and IFNγ mice 10 months. b , H&E staining of the kidneys from indicated mice ( n as indicated). The green arrows indicated mesangial stromal hyperplasia and the red arrows endothelial and mesangial proliferation. Mice were age matched to 8–14 months and the data pooled from three independent experiments. c , H&E staining of the spleens from the indicated mice ( n = 5). The green arrows indicated the extramedullary hematopoietic cells and the red arrows the inflammatory cells. Mice were age matched to 8 months. d , H&E staining of the lungs from the indicated mice ( n = 3). The yellow arrows (granulocytes) and red arrow (lymphocytes) indicated inflammatory cell infiltration; mice were aged 14 months. e , H&E staining of the pancreata from the indicated mice ( n = 3). The red arrows indicate inflammatory cell infiltration (granulocytes or lymphocytes) and the mice were aged 14 months. The graphs show the pathological disease scores. f , RT–qPCR analysis of Mx1 , Isg20l2 , Ifit1 and <t>Irf7</t> mRNA in kidney tissues from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as mean with s.d. The exact P values are shown.
    Anti Phospho Irf7 Ser437 438, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Genetic variants in UNC93B1 predispose to childhood-onset systemic lupus erythematosus"

    Article Title: Genetic variants in UNC93B1 predispose to childhood-onset systemic lupus erythematosus

    Journal: Nature Immunology

    doi: 10.1038/s41590-024-01846-5

    a , Serum IL-12p70, IP-10, IL-6 and IFNγ of the indicated mice ( n = 5 for IL-12p70 and IP-10; n = 4 for IL-6 and IFNγ). IP-10 mice were aged 8 months and IL-12p70, IL-6 and IFNγ mice 10 months. b , H&E staining of the kidneys from indicated mice ( n as indicated). The green arrows indicated mesangial stromal hyperplasia and the red arrows endothelial and mesangial proliferation. Mice were age matched to 8–14 months and the data pooled from three independent experiments. c , H&E staining of the spleens from the indicated mice ( n = 5). The green arrows indicated the extramedullary hematopoietic cells and the red arrows the inflammatory cells. Mice were age matched to 8 months. d , H&E staining of the lungs from the indicated mice ( n = 3). The yellow arrows (granulocytes) and red arrow (lymphocytes) indicated inflammatory cell infiltration; mice were aged 14 months. e , H&E staining of the pancreata from the indicated mice ( n = 3). The red arrows indicate inflammatory cell infiltration (granulocytes or lymphocytes) and the mice were aged 14 months. The graphs show the pathological disease scores. f , RT–qPCR analysis of Mx1 , Isg20l2 , Ifit1 and Irf7 mRNA in kidney tissues from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as mean with s.d. The exact P values are shown.
    Figure Legend Snippet: a , Serum IL-12p70, IP-10, IL-6 and IFNγ of the indicated mice ( n = 5 for IL-12p70 and IP-10; n = 4 for IL-6 and IFNγ). IP-10 mice were aged 8 months and IL-12p70, IL-6 and IFNγ mice 10 months. b , H&E staining of the kidneys from indicated mice ( n as indicated). The green arrows indicated mesangial stromal hyperplasia and the red arrows endothelial and mesangial proliferation. Mice were age matched to 8–14 months and the data pooled from three independent experiments. c , H&E staining of the spleens from the indicated mice ( n = 5). The green arrows indicated the extramedullary hematopoietic cells and the red arrows the inflammatory cells. Mice were age matched to 8 months. d , H&E staining of the lungs from the indicated mice ( n = 3). The yellow arrows (granulocytes) and red arrow (lymphocytes) indicated inflammatory cell infiltration; mice were aged 14 months. e , H&E staining of the pancreata from the indicated mice ( n = 3). The red arrows indicate inflammatory cell infiltration (granulocytes or lymphocytes) and the mice were aged 14 months. The graphs show the pathological disease scores. f , RT–qPCR analysis of Mx1 , Isg20l2 , Ifit1 and Irf7 mRNA in kidney tissues from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as mean with s.d. The exact P values are shown.

    Techniques Used: Staining, Quantitative RT-PCR, Two Tailed Test

    a , RT–qPCR analysis of Irf7 and Ifit1 mRNA in BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. b , Levels of phosphorylated NF-κB, JNK, P38, IRF5 and IRF7, as measured by immunoblotting, in lysates of BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. c , RNA-seq performed for UNC93B1 V117L BMDMs compared with controls (UNC93B1 WT ) ( n = 3 biological replicates). Mice were age matched to 8 months. Gene set enrichment analysis significantly associated with SLE. d , RT–qPCR analysis of Ifit1 , I rf7 , Isg-15 and Tnf mRNA expression in the BMDMs from the indicated mice ( n = 3) after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. e , Production of CXCL1, CCL5 and IP-10 in the supernatant of mice BMDMs isolated from the indicated mice ( n = 3), measured by CBA after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as the mean with s.d. The exact P values are shown.
    Figure Legend Snippet: a , RT–qPCR analysis of Irf7 and Ifit1 mRNA in BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. b , Levels of phosphorylated NF-κB, JNK, P38, IRF5 and IRF7, as measured by immunoblotting, in lysates of BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. c , RNA-seq performed for UNC93B1 V117L BMDMs compared with controls (UNC93B1 WT ) ( n = 3 biological replicates). Mice were age matched to 8 months. Gene set enrichment analysis significantly associated with SLE. d , RT–qPCR analysis of Ifit1 , I rf7 , Isg-15 and Tnf mRNA expression in the BMDMs from the indicated mice ( n = 3) after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. e , Production of CXCL1, CCL5 and IP-10 in the supernatant of mice BMDMs isolated from the indicated mice ( n = 3), measured by CBA after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as the mean with s.d. The exact P values are shown.

    Techniques Used: Quantitative RT-PCR, Western Blot, RNA Sequencing Assay, Expressing, Isolation, Two Tailed Test

    phospho irf3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf3
    a Glucose uptake in mouse adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). b Western blot showing phosphorylation of murine <t>IRF3</t> (S388) in mouse adipocytes after 30 min of LPS (700 ng/ml) treatment. c Glucose uptake in mouse adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). d Western blot of pAKT (S473) and IRF3, e Glucose uptake ( n = 6) in WT and FI3KO SVF-derived adipocytes treated with control or 100 nM insulin. f Glucose uptake in human SGBS adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). g Western blot showing phosphorylation of human IRF3 (S396) in human SGBS adipocytes after 30 mins of LPS (700 ng/ml) treatment. h Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or a scrambled control hairpin (shScr). i Glucose uptake in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). Statistical significance was assessed by three-way ANOVA ( c and i ) or two-way ANOVA ( a, d, e, f , and h ). Data are expressed as mean ± SEM.
    Phospho Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels"

    Article Title: Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels

    Journal: Nature Communications

    doi: 10.1038/s41467-024-48220-5

    a Glucose uptake in mouse adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). b Western blot showing phosphorylation of murine IRF3 (S388) in mouse adipocytes after 30 min of LPS (700 ng/ml) treatment. c Glucose uptake in mouse adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). d Western blot of pAKT (S473) and IRF3, e Glucose uptake ( n = 6) in WT and FI3KO SVF-derived adipocytes treated with control or 100 nM insulin. f Glucose uptake in human SGBS adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). g Western blot showing phosphorylation of human IRF3 (S396) in human SGBS adipocytes after 30 mins of LPS (700 ng/ml) treatment. h Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or a scrambled control hairpin (shScr). i Glucose uptake in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). Statistical significance was assessed by three-way ANOVA ( c and i ) or two-way ANOVA ( a, d, e, f , and h ). Data are expressed as mean ± SEM.
    Figure Legend Snippet: a Glucose uptake in mouse adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). b Western blot showing phosphorylation of murine IRF3 (S388) in mouse adipocytes after 30 min of LPS (700 ng/ml) treatment. c Glucose uptake in mouse adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). d Western blot of pAKT (S473) and IRF3, e Glucose uptake ( n = 6) in WT and FI3KO SVF-derived adipocytes treated with control or 100 nM insulin. f Glucose uptake in human SGBS adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). g Western blot showing phosphorylation of human IRF3 (S396) in human SGBS adipocytes after 30 mins of LPS (700 ng/ml) treatment. h Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or a scrambled control hairpin (shScr). i Glucose uptake in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). Statistical significance was assessed by three-way ANOVA ( c and i ) or two-way ANOVA ( a, d, e, f , and h ). Data are expressed as mean ± SEM.

    Techniques Used: Western Blot, Transduction, Expressing, shRNA, Derivative Assay

    a Glucose uptake in mouse adipocytes expressing GFP or constitutively active IRF3-2D mutant ( n = 8). b Western blot of pAKT (S473) and IRF3 in WT and FI3OE SVF-derived adipocytes treated with control or 100 nM insulin. c , Glucose uptake ( n = 6) in WT and FI3OE SVF-derived adipocytes treated with vehicle or 100 nM insulin. d Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing GFP or IRF3-2D. e Glucose uptake in human SGBS adipocytes expressing GFP or IRF3-2D mutant ( n = 8). Statistical significance was assessed by two-way ANOVA. Data are expressed as mean ± SEM.
    Figure Legend Snippet: a Glucose uptake in mouse adipocytes expressing GFP or constitutively active IRF3-2D mutant ( n = 8). b Western blot of pAKT (S473) and IRF3 in WT and FI3OE SVF-derived adipocytes treated with control or 100 nM insulin. c , Glucose uptake ( n = 6) in WT and FI3OE SVF-derived adipocytes treated with vehicle or 100 nM insulin. d Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing GFP or IRF3-2D. e Glucose uptake in human SGBS adipocytes expressing GFP or IRF3-2D mutant ( n = 8). Statistical significance was assessed by two-way ANOVA. Data are expressed as mean ± SEM.

    Techniques Used: Expressing, Mutagenesis, Western Blot, Derivative Assay, Transduction

    a Western blot of pAKT (S473) in WT SVF-derived adipocytes pre-treated with indicated conditioned medium plus insulin (100 nM) for 30 min. b Scheme showing the experimental paradigm used to identify IRF3 target genes by RNA-sequencing. Created with Biorender.com. c Volcano plot of RNA-seq dataset from ( b ). Genes that meet both significance and abundance are shown in black. Irf3 and Aig1 are marked in red. mRNA levels of Aig1 ( n = 3) ( d ) and western blot ( e ) of AIG1 in WT and FI3KO, FI3OE SVF-derived adipocytes. mRNA levels of Aig1 in iWAT, eWAT, and BAT from WT, FI3KO ( f ) and FI3OE mice ( g ) ( n = 5). h Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3KO mice ( n = 3). i Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3OE mice ( n = 3). Statistical significance was assessed by two-tailed Student’s t test. Data in all panels are expressed as mean ± SEM.
    Figure Legend Snippet: a Western blot of pAKT (S473) in WT SVF-derived adipocytes pre-treated with indicated conditioned medium plus insulin (100 nM) for 30 min. b Scheme showing the experimental paradigm used to identify IRF3 target genes by RNA-sequencing. Created with Biorender.com. c Volcano plot of RNA-seq dataset from ( b ). Genes that meet both significance and abundance are shown in black. Irf3 and Aig1 are marked in red. mRNA levels of Aig1 ( n = 3) ( d ) and western blot ( e ) of AIG1 in WT and FI3KO, FI3OE SVF-derived adipocytes. mRNA levels of Aig1 in iWAT, eWAT, and BAT from WT, FI3KO ( f ) and FI3OE mice ( g ) ( n = 5). h Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3KO mice ( n = 3). i Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3OE mice ( n = 3). Statistical significance was assessed by two-tailed Student’s t test. Data in all panels are expressed as mean ± SEM.

    Techniques Used: Western Blot, Derivative Assay, RNA Sequencing Assay, Two Tailed Test

    phospho irf3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf3
    Primer pairs for the real-time PCR.
    Phospho Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho irf3 - by Bioz Stars, 2024-06
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    1) Product Images from "Cytosolic DNA sensors activation of human astrocytes inhibits herpes simplex virus through IRF1 induction"

    Article Title: Cytosolic DNA sensors activation of human astrocytes inhibits herpes simplex virus through IRF1 induction

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2024.1383811

    Primer pairs for the real-time PCR.
    Figure Legend Snippet: Primer pairs for the real-time PCR.

    Techniques Used: Sequencing

    phospho irf3 ser386  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf3 ser386
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Phospho Irf3 Ser386, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Pseudorabies virus UL38 attenuates the cGAS-STING signaling pathway by recruiting Tollip to promote STING for autophagy degradation"

    Article Title: Pseudorabies virus UL38 attenuates the cGAS-STING signaling pathway by recruiting Tollip to promote STING for autophagy degradation

    Journal: Virology Journal

    doi: 10.1186/s12985-024-02379-x

    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, IRF3 and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Figure Legend Snippet: UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, IRF3 and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control

    Techniques Used: Transfection, Western Blot, Expressing

    phospho irf 3 ser396 4d4g rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf 3 ser396 4d4g rabbit mab
    Phospho Irf 3 Ser396 4d4g Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho irf 3 ser396 4d4g rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf 3 ser396 4d4g rabbit mab
    a Schematic illustration of the experimentation: transfection of CVB-FL and CVB-5’TD (5’TD8, 5’TD15, 5’TD21 and 5’TD50) synthetic RNA forms into human cardiac myocytes cells (HCM). b Viral load (RT-qPCR) in HCM transfected cells with CVB-FL and CVB-5’TD (5’TD8, 5’TD15, 5’TD21 and 5’TD50) synthetic RNA at 8 hours post-transfection. c IFN-β cytokine levels (ELISA) in supernatants of HCM at 8 HPT of various CVB-TD/or full-length RNA forms. d Analysis of eIF4G cleavage, and levels of phosphorylated IRF3 (pS386-IRF3) and IRF3 on a Western blot prepared with HCM cells collected at 8 HPT. Poly(I:C) is used as positive control of IFN-β pathway activation. Data represent mean ± SD (n = 3) (Mann-Whitney U test; *: p<0.05; ****: p<0.0001 and ns: non-significant). eIF4G: eukaryotic translation initiation factor 4 G; HMW: high molecular weight Poly(I:C); IRF3: Interferon Regulatory Factor 3; eIF4G: eukaryotic initiation factor 4 gamma; UT: untransfected.
    Phospho Irf 3 Ser396 4d4g Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Major Group-B Enterovirus populations deleted in the noncoding 5’ region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis"

    Article Title: Major Group-B Enterovirus populations deleted in the noncoding 5’ region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1012125

    a Schematic illustration of the experimentation: transfection of CVB-FL and CVB-5’TD (5’TD8, 5’TD15, 5’TD21 and 5’TD50) synthetic RNA forms into human cardiac myocytes cells (HCM). b Viral load (RT-qPCR) in HCM transfected cells with CVB-FL and CVB-5’TD (5’TD8, 5’TD15, 5’TD21 and 5’TD50) synthetic RNA at 8 hours post-transfection. c IFN-β cytokine levels (ELISA) in supernatants of HCM at 8 HPT of various CVB-TD/or full-length RNA forms. d Analysis of eIF4G cleavage, and levels of phosphorylated IRF3 (pS386-IRF3) and IRF3 on a Western blot prepared with HCM cells collected at 8 HPT. Poly(I:C) is used as positive control of IFN-β pathway activation. Data represent mean ± SD (n = 3) (Mann-Whitney U test; *: p<0.05; ****: p<0.0001 and ns: non-significant). eIF4G: eukaryotic translation initiation factor 4 G; HMW: high molecular weight Poly(I:C); IRF3: Interferon Regulatory Factor 3; eIF4G: eukaryotic initiation factor 4 gamma; UT: untransfected.
    Figure Legend Snippet: a Schematic illustration of the experimentation: transfection of CVB-FL and CVB-5’TD (5’TD8, 5’TD15, 5’TD21 and 5’TD50) synthetic RNA forms into human cardiac myocytes cells (HCM). b Viral load (RT-qPCR) in HCM transfected cells with CVB-FL and CVB-5’TD (5’TD8, 5’TD15, 5’TD21 and 5’TD50) synthetic RNA at 8 hours post-transfection. c IFN-β cytokine levels (ELISA) in supernatants of HCM at 8 HPT of various CVB-TD/or full-length RNA forms. d Analysis of eIF4G cleavage, and levels of phosphorylated IRF3 (pS386-IRF3) and IRF3 on a Western blot prepared with HCM cells collected at 8 HPT. Poly(I:C) is used as positive control of IFN-β pathway activation. Data represent mean ± SD (n = 3) (Mann-Whitney U test; *: p<0.05; ****: p<0.0001 and ns: non-significant). eIF4G: eukaryotic translation initiation factor 4 G; HMW: high molecular weight Poly(I:C); IRF3: Interferon Regulatory Factor 3; eIF4G: eukaryotic initiation factor 4 gamma; UT: untransfected.

    Techniques Used: Transfection, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Positive Control, Activation Assay, MANN-WHITNEY, Molecular Weight

    phospho irf3 ser396  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf3 ser396
    Phospho Irf3 Ser396, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho irf 3 rabbit mab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf 3 rabbit mab
    Phospho Irf 3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phospho irf 3 ser396  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho irf 3 ser396
    Phospho Irf 3 Ser396, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phospho irf3  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho irf3
    Anti Phospho Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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  • 86
    Cell Signaling Technology Inc anti phospho irf7 ser437 438
    a , Serum IL-12p70, IP-10, IL-6 and IFNγ of the indicated mice ( n = 5 for IL-12p70 and IP-10; n = 4 for IL-6 and IFNγ). IP-10 mice were aged 8 months and IL-12p70, IL-6 and IFNγ mice 10 months. b , H&E staining of the kidneys from indicated mice ( n as indicated). The green arrows indicated mesangial stromal hyperplasia and the red arrows endothelial and mesangial proliferation. Mice were age matched to 8–14 months and the data pooled from three independent experiments. c , H&E staining of the spleens from the indicated mice ( n = 5). The green arrows indicated the extramedullary hematopoietic cells and the red arrows the inflammatory cells. Mice were age matched to 8 months. d , H&E staining of the lungs from the indicated mice ( n = 3). The yellow arrows (granulocytes) and red arrow (lymphocytes) indicated inflammatory cell infiltration; mice were aged 14 months. e , H&E staining of the pancreata from the indicated mice ( n = 3). The red arrows indicate inflammatory cell infiltration (granulocytes or lymphocytes) and the mice were aged 14 months. The graphs show the pathological disease scores. f , RT–qPCR analysis of Mx1 , Isg20l2 , Ifit1 and <t>Irf7</t> mRNA in kidney tissues from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as mean with s.d. The exact P values are shown.
    Anti Phospho Irf7 Ser437 438, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho irf7 ser437 438/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho irf7 ser437 438 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc phospho irf3
    a Glucose uptake in mouse adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). b Western blot showing phosphorylation of murine <t>IRF3</t> (S388) in mouse adipocytes after 30 min of LPS (700 ng/ml) treatment. c Glucose uptake in mouse adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). d Western blot of pAKT (S473) and IRF3, e Glucose uptake ( n = 6) in WT and FI3KO SVF-derived adipocytes treated with control or 100 nM insulin. f Glucose uptake in human SGBS adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). g Western blot showing phosphorylation of human IRF3 (S396) in human SGBS adipocytes after 30 mins of LPS (700 ng/ml) treatment. h Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or a scrambled control hairpin (shScr). i Glucose uptake in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). Statistical significance was assessed by three-way ANOVA ( c and i ) or two-way ANOVA ( a, d, e, f , and h ). Data are expressed as mean ± SEM.
    Phospho Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho irf3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    phospho irf3 - by Bioz Stars, 2024-06
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    86
    Cell Signaling Technology Inc phospho irf3 ser386
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Phospho Irf3 Ser386, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho irf3 ser386/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho irf3 ser386 - by Bioz Stars, 2024-06
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    Cell Signaling Technology Inc phospho irf 3 ser396 4d4g rabbit mab
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Phospho Irf 3 Ser396 4d4g Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho irf 3 ser396 4d4g rabbit mab/product/Cell Signaling Technology Inc
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    86
    Cell Signaling Technology Inc phospho irf3 ser396
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Phospho Irf3 Ser396, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho irf3 ser396/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho irf3 ser396 - by Bioz Stars, 2024-06
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    Cell Signaling Technology Inc phospho irf 3 rabbit mab
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Phospho Irf 3 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho irf 3 rabbit mab/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc phospho irf 3 ser396
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Phospho Irf 3 Ser396, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho irf 3 ser396/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    phospho irf 3 ser396 - by Bioz Stars, 2024-06
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    Cell Signaling Technology Inc anti phospho irf3
    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, <t>IRF3</t> and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control
    Anti Phospho Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho irf3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho irf3 - by Bioz Stars, 2024-06
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    Image Search Results


    a , Serum IL-12p70, IP-10, IL-6 and IFNγ of the indicated mice ( n = 5 for IL-12p70 and IP-10; n = 4 for IL-6 and IFNγ). IP-10 mice were aged 8 months and IL-12p70, IL-6 and IFNγ mice 10 months. b , H&E staining of the kidneys from indicated mice ( n as indicated). The green arrows indicated mesangial stromal hyperplasia and the red arrows endothelial and mesangial proliferation. Mice were age matched to 8–14 months and the data pooled from three independent experiments. c , H&E staining of the spleens from the indicated mice ( n = 5). The green arrows indicated the extramedullary hematopoietic cells and the red arrows the inflammatory cells. Mice were age matched to 8 months. d , H&E staining of the lungs from the indicated mice ( n = 3). The yellow arrows (granulocytes) and red arrow (lymphocytes) indicated inflammatory cell infiltration; mice were aged 14 months. e , H&E staining of the pancreata from the indicated mice ( n = 3). The red arrows indicate inflammatory cell infiltration (granulocytes or lymphocytes) and the mice were aged 14 months. The graphs show the pathological disease scores. f , RT–qPCR analysis of Mx1 , Isg20l2 , Ifit1 and Irf7 mRNA in kidney tissues from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as mean with s.d. The exact P values are shown.

    Journal: Nature Immunology

    Article Title: Genetic variants in UNC93B1 predispose to childhood-onset systemic lupus erythematosus

    doi: 10.1038/s41590-024-01846-5

    Figure Lengend Snippet: a , Serum IL-12p70, IP-10, IL-6 and IFNγ of the indicated mice ( n = 5 for IL-12p70 and IP-10; n = 4 for IL-6 and IFNγ). IP-10 mice were aged 8 months and IL-12p70, IL-6 and IFNγ mice 10 months. b , H&E staining of the kidneys from indicated mice ( n as indicated). The green arrows indicated mesangial stromal hyperplasia and the red arrows endothelial and mesangial proliferation. Mice were age matched to 8–14 months and the data pooled from three independent experiments. c , H&E staining of the spleens from the indicated mice ( n = 5). The green arrows indicated the extramedullary hematopoietic cells and the red arrows the inflammatory cells. Mice were age matched to 8 months. d , H&E staining of the lungs from the indicated mice ( n = 3). The yellow arrows (granulocytes) and red arrow (lymphocytes) indicated inflammatory cell infiltration; mice were aged 14 months. e , H&E staining of the pancreata from the indicated mice ( n = 3). The red arrows indicate inflammatory cell infiltration (granulocytes or lymphocytes) and the mice were aged 14 months. The graphs show the pathological disease scores. f , RT–qPCR analysis of Mx1 , Isg20l2 , Ifit1 and Irf7 mRNA in kidney tissues from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as mean with s.d. The exact P values are shown.

    Article Snippet: For immunoblots, the following antibodies were used: anti-FLAG (Sigma-Aldrich, cat. no. F1804), anti-NF-κB p65 (Cell Signaling, cat. no. 8242S), anti-phospho-NF-κB p65 (Ser468) (Cell Signaling, cat. no. 3039S), anti-JNK (Abcam, cat. no. ab179461), anti-phospho-JNK (Abcam, cat. no. ab124956), anti-P38 (Cell Signaling, cat. no. 8690), anti-phospho-P38 (Cell Signaling, cat. no. 9211S), anti-IRF5 (Cell Signaling, cat. no. 20261), anti-phospho-IRF5 (Ser437) (Invitrogen, cat. no. PA5-64760), anti-IRAK4 (Cell Signaling, cat. no. 4363), anti-phospho-IRAK4 (Thr345/Ser346) (Cell Signaling, cat. no. 11927), anti-p44/42 MAPK (ERK1/2) (Cell Signaling, cat. no. 9102S), anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (Cell Signaling, cat. no. 9101S), anti-IRF7 (Cell Signaling, cat. no. 39659), anti-phospho-IRF7 (Ser437/438) (Cell Signaling, cat. no. 24129), anti-p-Ser/phosphoserine (Santa Cruz Biotechnology, cat. no. sc-81514), anti-β-actin (ABclonal, cat. no. AC026), anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (Cell Signaling, cat. no. 7074) and anti-mouse IgG HRP-linked antibody (Cell Signaling, cat. no. 7076).

    Techniques: Staining, Quantitative RT-PCR, Two Tailed Test

    a , RT–qPCR analysis of Irf7 and Ifit1 mRNA in BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. b , Levels of phosphorylated NF-κB, JNK, P38, IRF5 and IRF7, as measured by immunoblotting, in lysates of BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. c , RNA-seq performed for UNC93B1 V117L BMDMs compared with controls (UNC93B1 WT ) ( n = 3 biological replicates). Mice were age matched to 8 months. Gene set enrichment analysis significantly associated with SLE. d , RT–qPCR analysis of Ifit1 , I rf7 , Isg-15 and Tnf mRNA expression in the BMDMs from the indicated mice ( n = 3) after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. e , Production of CXCL1, CCL5 and IP-10 in the supernatant of mice BMDMs isolated from the indicated mice ( n = 3), measured by CBA after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as the mean with s.d. The exact P values are shown.

    Journal: Nature Immunology

    Article Title: Genetic variants in UNC93B1 predispose to childhood-onset systemic lupus erythematosus

    doi: 10.1038/s41590-024-01846-5

    Figure Lengend Snippet: a , RT–qPCR analysis of Irf7 and Ifit1 mRNA in BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. b , Levels of phosphorylated NF-κB, JNK, P38, IRF5 and IRF7, as measured by immunoblotting, in lysates of BMDMs from the indicated mice ( n = 3). Mice were age matched to 8 months. c , RNA-seq performed for UNC93B1 V117L BMDMs compared with controls (UNC93B1 WT ) ( n = 3 biological replicates). Mice were age matched to 8 months. Gene set enrichment analysis significantly associated with SLE. d , RT–qPCR analysis of Ifit1 , I rf7 , Isg-15 and Tnf mRNA expression in the BMDMs from the indicated mice ( n = 3) after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired Student’s t -tests. The exact P values are shown. e , Production of CXCL1, CCL5 and IP-10 in the supernatant of mice BMDMs isolated from the indicated mice ( n = 3), measured by CBA after stimulation by 40 μg ml −1 of HMW poly(I:C), 2 μg ml −1 of R848 and 10 μg ml −1 of CPG-C for 24 h. Mice were age matched to 14 months. Statistical analysis was done using unpaired, two-tailed Student’s t -tests and data are presented as the mean with s.d. The exact P values are shown.

    Article Snippet: For immunoblots, the following antibodies were used: anti-FLAG (Sigma-Aldrich, cat. no. F1804), anti-NF-κB p65 (Cell Signaling, cat. no. 8242S), anti-phospho-NF-κB p65 (Ser468) (Cell Signaling, cat. no. 3039S), anti-JNK (Abcam, cat. no. ab179461), anti-phospho-JNK (Abcam, cat. no. ab124956), anti-P38 (Cell Signaling, cat. no. 8690), anti-phospho-P38 (Cell Signaling, cat. no. 9211S), anti-IRF5 (Cell Signaling, cat. no. 20261), anti-phospho-IRF5 (Ser437) (Invitrogen, cat. no. PA5-64760), anti-IRAK4 (Cell Signaling, cat. no. 4363), anti-phospho-IRAK4 (Thr345/Ser346) (Cell Signaling, cat. no. 11927), anti-p44/42 MAPK (ERK1/2) (Cell Signaling, cat. no. 9102S), anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (Cell Signaling, cat. no. 9101S), anti-IRF7 (Cell Signaling, cat. no. 39659), anti-phospho-IRF7 (Ser437/438) (Cell Signaling, cat. no. 24129), anti-p-Ser/phosphoserine (Santa Cruz Biotechnology, cat. no. sc-81514), anti-β-actin (ABclonal, cat. no. AC026), anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (Cell Signaling, cat. no. 7074) and anti-mouse IgG HRP-linked antibody (Cell Signaling, cat. no. 7076).

    Techniques: Quantitative RT-PCR, Western Blot, RNA Sequencing Assay, Expressing, Isolation, Two Tailed Test

    a Glucose uptake in mouse adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). b Western blot showing phosphorylation of murine IRF3 (S388) in mouse adipocytes after 30 min of LPS (700 ng/ml) treatment. c Glucose uptake in mouse adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). d Western blot of pAKT (S473) and IRF3, e Glucose uptake ( n = 6) in WT and FI3KO SVF-derived adipocytes treated with control or 100 nM insulin. f Glucose uptake in human SGBS adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). g Western blot showing phosphorylation of human IRF3 (S396) in human SGBS adipocytes after 30 mins of LPS (700 ng/ml) treatment. h Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or a scrambled control hairpin (shScr). i Glucose uptake in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). Statistical significance was assessed by three-way ANOVA ( c and i ) or two-way ANOVA ( a, d, e, f , and h ). Data are expressed as mean ± SEM.

    Journal: Nature Communications

    Article Title: Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels

    doi: 10.1038/s41467-024-48220-5

    Figure Lengend Snippet: a Glucose uptake in mouse adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). b Western blot showing phosphorylation of murine IRF3 (S388) in mouse adipocytes after 30 min of LPS (700 ng/ml) treatment. c Glucose uptake in mouse adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). d Western blot of pAKT (S473) and IRF3, e Glucose uptake ( n = 6) in WT and FI3KO SVF-derived adipocytes treated with control or 100 nM insulin. f Glucose uptake in human SGBS adipocytes after treatment with varying doses of LPS for 2 days ( n = 8). g Western blot showing phosphorylation of human IRF3 (S396) in human SGBS adipocytes after 30 mins of LPS (700 ng/ml) treatment. h Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or a scrambled control hairpin (shScr). i Glucose uptake in human SGBS adipocytes transduced with lentivirus expressing shRNA against Irf3 or shScr control hairpin in the absence or presence of LPS (700 ng/ml) ( n = 8). Statistical significance was assessed by three-way ANOVA ( c and i ) or two-way ANOVA ( a, d, e, f , and h ). Data are expressed as mean ± SEM.

    Article Snippet: Primary antibodies against phospho AKT(Ser473) (CST, 9271), AKT (AKT, 4685), HSP90 (CST, 4877), phospho IRF3(Ser396) (CST, 29047), IRF3 (CST, 11904) and AIG1 (Proteintech, 14468-1-AP), were used.

    Techniques: Western Blot, Transduction, Expressing, shRNA, Derivative Assay

    a Glucose uptake in mouse adipocytes expressing GFP or constitutively active IRF3-2D mutant ( n = 8). b Western blot of pAKT (S473) and IRF3 in WT and FI3OE SVF-derived adipocytes treated with control or 100 nM insulin. c , Glucose uptake ( n = 6) in WT and FI3OE SVF-derived adipocytes treated with vehicle or 100 nM insulin. d Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing GFP or IRF3-2D. e Glucose uptake in human SGBS adipocytes expressing GFP or IRF3-2D mutant ( n = 8). Statistical significance was assessed by two-way ANOVA. Data are expressed as mean ± SEM.

    Journal: Nature Communications

    Article Title: Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels

    doi: 10.1038/s41467-024-48220-5

    Figure Lengend Snippet: a Glucose uptake in mouse adipocytes expressing GFP or constitutively active IRF3-2D mutant ( n = 8). b Western blot of pAKT (S473) and IRF3 in WT and FI3OE SVF-derived adipocytes treated with control or 100 nM insulin. c , Glucose uptake ( n = 6) in WT and FI3OE SVF-derived adipocytes treated with vehicle or 100 nM insulin. d Western blot of pAKT (S473) and IRF3 in human SGBS adipocytes transduced with lentivirus expressing GFP or IRF3-2D. e Glucose uptake in human SGBS adipocytes expressing GFP or IRF3-2D mutant ( n = 8). Statistical significance was assessed by two-way ANOVA. Data are expressed as mean ± SEM.

    Article Snippet: Primary antibodies against phospho AKT(Ser473) (CST, 9271), AKT (AKT, 4685), HSP90 (CST, 4877), phospho IRF3(Ser396) (CST, 29047), IRF3 (CST, 11904) and AIG1 (Proteintech, 14468-1-AP), were used.

    Techniques: Expressing, Mutagenesis, Western Blot, Derivative Assay, Transduction

    a Western blot of pAKT (S473) in WT SVF-derived adipocytes pre-treated with indicated conditioned medium plus insulin (100 nM) for 30 min. b Scheme showing the experimental paradigm used to identify IRF3 target genes by RNA-sequencing. Created with Biorender.com. c Volcano plot of RNA-seq dataset from ( b ). Genes that meet both significance and abundance are shown in black. Irf3 and Aig1 are marked in red. mRNA levels of Aig1 ( n = 3) ( d ) and western blot ( e ) of AIG1 in WT and FI3KO, FI3OE SVF-derived adipocytes. mRNA levels of Aig1 in iWAT, eWAT, and BAT from WT, FI3KO ( f ) and FI3OE mice ( g ) ( n = 5). h Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3KO mice ( n = 3). i Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3OE mice ( n = 3). Statistical significance was assessed by two-tailed Student’s t test. Data in all panels are expressed as mean ± SEM.

    Journal: Nature Communications

    Article Title: Inflammation causes insulin resistance in mice via interferon regulatory factor 3 (IRF3)-mediated reduction in FAHFA levels

    doi: 10.1038/s41467-024-48220-5

    Figure Lengend Snippet: a Western blot of pAKT (S473) in WT SVF-derived adipocytes pre-treated with indicated conditioned medium plus insulin (100 nM) for 30 min. b Scheme showing the experimental paradigm used to identify IRF3 target genes by RNA-sequencing. Created with Biorender.com. c Volcano plot of RNA-seq dataset from ( b ). Genes that meet both significance and abundance are shown in black. Irf3 and Aig1 are marked in red. mRNA levels of Aig1 ( n = 3) ( d ) and western blot ( e ) of AIG1 in WT and FI3KO, FI3OE SVF-derived adipocytes. mRNA levels of Aig1 in iWAT, eWAT, and BAT from WT, FI3KO ( f ) and FI3OE mice ( g ) ( n = 5). h Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3KO mice ( n = 3). i Western blot of AIG1 in iWAT, eWAT, and BAT of WT and FI3OE mice ( n = 3). Statistical significance was assessed by two-tailed Student’s t test. Data in all panels are expressed as mean ± SEM.

    Article Snippet: Primary antibodies against phospho AKT(Ser473) (CST, 9271), AKT (AKT, 4685), HSP90 (CST, 4877), phospho IRF3(Ser396) (CST, 29047), IRF3 (CST, 11904) and AIG1 (Proteintech, 14468-1-AP), were used.

    Techniques: Western Blot, Derivative Assay, RNA Sequencing Assay, Two Tailed Test

    UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, IRF3 and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control

    Journal: Virology Journal

    Article Title: Pseudorabies virus UL38 attenuates the cGAS-STING signaling pathway by recruiting Tollip to promote STING for autophagy degradation

    doi: 10.1186/s12985-024-02379-x

    Figure Lengend Snippet: UL38 promotes decreasing of STING. PK15 cells were transfected with pCMV-Myc (1 µg) or Myc-UL38 (1 µg) for 24 h, then cells were stimulated with 1 MOI PRV ( A ), poly(dA: dT) (1 µg/mL) ( B ) or 2’3’-cGAMP (2 mg/mL) ( C ) respectively for another 12 h. Cells were collected for western blotting analysis. The expression of cGAS, STING, TBK1, IRF3 and phosphorylated IRF3 were detected. For all experiments, β-actin serves as a loading control

    Article Snippet: The antibodies for phospho-IRF3 (Ser386) (37829 S), STING (13647 S), TBK1 (3013 S), and Myc-Tag (9B11) Mouse mAb (2276 S) were purchased from Cell Signaling Technology.

    Techniques: Transfection, Western Blot, Expressing