rabbit monoclonal anti p i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti p i κ b α
    Effect of galangin on NF- <t>κ</t> <t>B</t> in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I <t>κ</t> <t>B</t> <t>α</t> , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.
    Rabbit Monoclonal Anti P I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti p i κ b α/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti p i κ b α - by Bioz Stars, 2023-03
    99/100 stars

    Images

    1) Product Images from "Galangin Suppresses Renal Inflammation via the Inhibition of NF- κ B, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells"

    Article Title: Galangin Suppresses Renal Inflammation via the Inhibition of NF- κ B, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    Journal: BioMed Research International

    doi: 10.1155/2019/3018357

    Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.
    Figure Legend Snippet: Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.

    Techniques Used: Expressing

    anti phospho i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho i κ b α
    Effect of DEX on the expression levels of the TLR4/NF- <t>κ</t> <t>B</t> signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I <t>κ</t> <t>B</t> <t>α</t> , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
    Anti Phospho I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho i κ b α/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti phospho i κ b α - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "Dexmedetomidine Protects against Airway Inflammation and Airway Remodeling in a Murine Model of Chronic Asthma through TLR4/NF- κ B Signaling Pathway"

    Article Title: Dexmedetomidine Protects against Airway Inflammation and Airway Remodeling in a Murine Model of Chronic Asthma through TLR4/NF- κ B Signaling Pathway

    Journal: Mediators of Inflammation

    doi: 10.1155/2023/3695469

    Effect of DEX on the expression levels of the TLR4/NF- κ B signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I κ B α , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
    Figure Legend Snippet: Effect of DEX on the expression levels of the TLR4/NF- κ B signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I κ B α , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

    Techniques Used: Expressing, Western Blot

    anti phospho i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho i κ b α
    Myrislignan regulated the expression of EMT-related genes and inhibited the phosphorylation of p65 protein. (a–d) The protein (E-cadherin, Snail1, and Slug) expression was detected via western blot assay after myrislignan treatment in U87 and U251 cells. (e–j) The protein (total p65/I κ B- α and phosphorylated p65/I κ B- α ) levels were measured via western blot assay after myrislignan treatment in U87 and U251 cells. (k) Immunofluorescence of p65 and p-p65 protein after myrislignan treatment in U87 cells. (l, m) Dual-luciferase reporter assay was conducted to evaluate the status of NF- <t>κ</t> <t>B</t> signal in GBM cells treated with myrislignan. Scale bar, 10 μ m. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ns: no significance.
    Anti Phospho I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho i κ b α/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    anti phospho i κ b α - by Bioz Stars, 2023-03
    86/100 stars

    Images

    1) Product Images from "NF- κ B Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner"

    Article Title: NF- κ B Inhibitor Myrislignan Induces Ferroptosis of Glioblastoma Cells via Regulating Epithelial-Mesenchymal Transformation in a Slug-Dependent Manner

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2023/7098313

    Myrislignan regulated the expression of EMT-related genes and inhibited the phosphorylation of p65 protein. (a–d) The protein (E-cadherin, Snail1, and Slug) expression was detected via western blot assay after myrislignan treatment in U87 and U251 cells. (e–j) The protein (total p65/I κ B- α and phosphorylated p65/I κ B- α ) levels were measured via western blot assay after myrislignan treatment in U87 and U251 cells. (k) Immunofluorescence of p65 and p-p65 protein after myrislignan treatment in U87 cells. (l, m) Dual-luciferase reporter assay was conducted to evaluate the status of NF- κ B signal in GBM cells treated with myrislignan. Scale bar, 10 μ m. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ns: no significance.
    Figure Legend Snippet: Myrislignan regulated the expression of EMT-related genes and inhibited the phosphorylation of p65 protein. (a–d) The protein (E-cadherin, Snail1, and Slug) expression was detected via western blot assay after myrislignan treatment in U87 and U251 cells. (e–j) The protein (total p65/I κ B- α and phosphorylated p65/I κ B- α ) levels were measured via western blot assay after myrislignan treatment in U87 and U251 cells. (k) Immunofluorescence of p65 and p-p65 protein after myrislignan treatment in U87 cells. (l, m) Dual-luciferase reporter assay was conducted to evaluate the status of NF- κ B signal in GBM cells treated with myrislignan. Scale bar, 10 μ m. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001; ns: no significance.

    Techniques Used: Expressing, Western Blot, Immunofluorescence, Luciferase, Reporter Assay

    anti phospho i κ b α antibodies  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho i κ b α antibodies
    Anti Phospho I κ B α Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho i κ b α antibodies/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
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    86/100 stars

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    rabbit monoclonal anti p i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti p i κ b α
    Effect of galangin on NF- <t>κ</t> <t>B</t> in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I <t>κ</t> <t>B</t> <t>α</t> , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.
    Rabbit Monoclonal Anti P I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti p i κ b α/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti p i κ b α - by Bioz Stars, 2023-03
    99/100 stars

    Images

    1) Product Images from "Galangin Suppresses Renal Inflammation via the Inhibition of NF- κ B, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells"

    Article Title: Galangin Suppresses Renal Inflammation via the Inhibition of NF- κ B, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    Journal: BioMed Research International

    doi: 10.1155/2019/3018357

    Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.
    Figure Legend Snippet: Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.

    Techniques Used: Expressing

    anti phospho i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho i κ b α
    Deficiency in TRAF5 augments the activation of NF- <t>κ</t> <t>B</t> signaling. The phosphorylated and total protein expression levels of p65 and I <t>κ</t> <t>B</t> <t>α</t> were detected in the colons of TRAF5 KO and WT mice. (a) Representative blots. ((b), (c), (d), and (e)) Quantitative results. The data are representative of 3 independent experiments (mean ± SD). N = 4 per group. ∗ p < 0.05 versus the DSS-treated WT group; ∗∗ p < 0.01 versus the DSS-treated WT group. NS: not significant versus the DSS-treated WT group.
    Anti Phospho I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho i κ b α/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    anti phospho i κ b α - by Bioz Stars, 2023-03
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    Images

    1) Product Images from "Disruption of Tumor Necrosis Factor Receptor-Associated Factor 5 Exacerbates Murine Experimental Colitis via Regulating T Helper Cell-Mediated Inflammation"

    Article Title: Disruption of Tumor Necrosis Factor Receptor-Associated Factor 5 Exacerbates Murine Experimental Colitis via Regulating T Helper Cell-Mediated Inflammation

    Journal: Mediators of Inflammation

    doi: 10.1155/2016/9453745

    Deficiency in TRAF5 augments the activation of NF- κ B signaling. The phosphorylated and total protein expression levels of p65 and I κ B α were detected in the colons of TRAF5 KO and WT mice. (a) Representative blots. ((b), (c), (d), and (e)) Quantitative results. The data are representative of 3 independent experiments (mean ± SD). N = 4 per group. ∗ p < 0.05 versus the DSS-treated WT group; ∗∗ p < 0.01 versus the DSS-treated WT group. NS: not significant versus the DSS-treated WT group.
    Figure Legend Snippet: Deficiency in TRAF5 augments the activation of NF- κ B signaling. The phosphorylated and total protein expression levels of p65 and I κ B α were detected in the colons of TRAF5 KO and WT mice. (a) Representative blots. ((b), (c), (d), and (e)) Quantitative results. The data are representative of 3 independent experiments (mean ± SD). N = 4 per group. ∗ p < 0.05 versus the DSS-treated WT group; ∗∗ p < 0.01 versus the DSS-treated WT group. NS: not significant versus the DSS-treated WT group.

    Techniques Used: Activation Assay, Expressing

    anti phospho i κ b α  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
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    Cell Signaling Technology Inc anti phospho i κ b α
    The effect of apamin on NF- <t>κ</t> <t>B</t> signaling pathway in LPS-treated THP-1-derived macrophages. (a) Expression levels of IKK and I <t>κ</t> <t>B</t> <t>α</t> in the cytosolic fraction and NF- κ B in the nuclear fraction were determined by western blot. GAPDH and histone H2B were used as the internal controls for cytosolic and nuclear fraction loading control, respectively. (b) Nuclear NF- κ B activity was examined by EMSA. The arrow indicates the specific NF- κ B band. (c) Luciferase activity was measured with a luminometer. * P < 0.05 compared to control cells, # P < 0.05 compared to control cells treated with LPS alone.
    Anti Phospho I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86/100 stars

    Images

    1) Product Images from "The Protective Effect of Apamin on LPS/Fat-Induced Atherosclerotic Mice"

    Article Title: The Protective Effect of Apamin on LPS/Fat-Induced Atherosclerotic Mice

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2012/305454

    The effect of apamin on NF- κ B signaling pathway in LPS-treated THP-1-derived macrophages. (a) Expression levels of IKK and I κ B α in the cytosolic fraction and NF- κ B in the nuclear fraction were determined by western blot. GAPDH and histone H2B were used as the internal controls for cytosolic and nuclear fraction loading control, respectively. (b) Nuclear NF- κ B activity was examined by EMSA. The arrow indicates the specific NF- κ B band. (c) Luciferase activity was measured with a luminometer. * P < 0.05 compared to control cells, # P < 0.05 compared to control cells treated with LPS alone.
    Figure Legend Snippet: The effect of apamin on NF- κ B signaling pathway in LPS-treated THP-1-derived macrophages. (a) Expression levels of IKK and I κ B α in the cytosolic fraction and NF- κ B in the nuclear fraction were determined by western blot. GAPDH and histone H2B were used as the internal controls for cytosolic and nuclear fraction loading control, respectively. (b) Nuclear NF- κ B activity was examined by EMSA. The arrow indicates the specific NF- κ B band. (c) Luciferase activity was measured with a luminometer. * P < 0.05 compared to control cells, # P < 0.05 compared to control cells treated with LPS alone.

    Techniques Used: Derivative Assay, Expressing, Western Blot, Activity Assay, Luciferase

    anti phospho i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho i κ b α
    Anti Phospho I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    91/100 stars

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    mouse monoclonal anti i κ b α  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse monoclonal anti i κ b α
    Mouse Monoclonal Anti I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phos i κ b α ser32 antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phos i κ b α ser32 antibody
    Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α <t>(Ser32)</t> from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.
    Anti Phos I κ B α Ser32 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phos i κ b α ser32 antibody/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
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    Images

    1) Product Images from "Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF- k B Signaling Pathway"

    Article Title: Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF- k B Signaling Pathway

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    doi: 10.1155/2021/8868527

    Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α (Ser32) from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.
    Figure Legend Snippet: Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α (Ser32) from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.

    Techniques Used: Western Blot

    phos i κ b α ser32  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phos i κ b α ser32
    Phos I κ B α Ser32, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc rabbit monoclonal anti p i κ b α
    Effect of galangin on NF- <t>κ</t> <t>B</t> in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I <t>κ</t> <t>B</t> <t>α</t> , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.
    Rabbit Monoclonal Anti P I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti p i κ b α/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti phospho i κ b α
    Effect of DEX on the expression levels of the TLR4/NF- <t>κ</t> <t>B</t> signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I <t>κ</t> <t>B</t> <t>α</t> , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
    Anti Phospho I κ B α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho i κ b α/product/Cell Signaling Technology Inc
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    Cell Signaling Technology Inc anti phospho i κ b α antibodies
    Effect of DEX on the expression levels of the TLR4/NF- <t>κ</t> <t>B</t> signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I <t>κ</t> <t>B</t> <t>α</t> , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
    Anti Phospho I κ B α Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of DEX on the expression levels of the TLR4/NF- <t>κ</t> <t>B</t> signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I <t>κ</t> <t>B</t> <t>α</t> , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.
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    Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α <t>(Ser32)</t> from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.
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    Cell Signaling Technology Inc phos i κ b α ser32
    Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α <t>(Ser32)</t> from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.
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    Image Search Results


    Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.

    Journal: BioMed Research International

    Article Title: Galangin Suppresses Renal Inflammation via the Inhibition of NF- κ B, PI3K/AKT and NLRP3 in Uric Acid Treated NRK-52E Tubular Epithelial Cells

    doi: 10.1155/2019/3018357

    Figure Lengend Snippet: Effect of galangin on NF- κ B in UA treated NRK-52E cells. Galangin decreased the expression of the phosphorylated form of I κ B α , IKK β , and p65, and inhibited I κ B α degradation in UA treated NRK-52E cells. Each of the protein bands was normalized to band of β -actin and reported as relative intensity. Results are expressed as mean ± SEM of three independent experiments. # p < 0.05, ## p < 0.01 vs control group; ∗p < 0.05, ∗∗p < 0.01 vs UA group.

    Article Snippet: The antibodies used were the following: rabbit monoclonal anti-p-I κ B α (#2859S), rabbit polyclonal anti-I κ B α (#9242S), rabbit polyclonal anti-p-p65(#3031S), rabbit monoclonal anti-p65(#8242S), rabbit monoclonal anti-p-Ikk β (#2078S), rabbit monoclonal anti-Ikk β (#8943S), rabbit monoclonal anti-NLRP3(#15101S), rabbit monoclonal anti-ASC(#67824S), rabbit polyclonal anti-caspase-1(#2225S), rabbit polyclonal anti-p-PI3K(#4228S), rabbit polyclonal anti-PI3K(#4292S), mouse monoclonal anti-p-AKT(#4051S), mouse monoclonal anti-AKT(#2920S), and rabbit polyclonal anti- β -actin(#4967S), and purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Expressing

    Effect of DEX on the expression levels of the TLR4/NF- κ B signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I κ B α , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

    Journal: Mediators of Inflammation

    Article Title: Dexmedetomidine Protects against Airway Inflammation and Airway Remodeling in a Murine Model of Chronic Asthma through TLR4/NF- κ B Signaling Pathway

    doi: 10.1155/2023/3695469

    Figure Lengend Snippet: Effect of DEX on the expression levels of the TLR4/NF- κ B signaling pathway in lung tissues. (a) Representative images of western blots of TLR4, phosphorylated- (p-) I κ B α , I κ B α , p-NF- κ B p65, and NF- κ B p65. (b–d) Densitometric analyses of TLR4 and the ratios of p-I κ B α /I κ B α and p-NF- κ B p65/NF- κ B p65 in lung tissues form each group. Data were presented as mean ± SEM ( n = 3~5 animals). ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

    Article Snippet: Primary antibodies used for western blot included anti-TLR4 (1 : 1000, 66351-1-Ig, Proteintech), anti-NF- κ B p65 (1 : 1000, 66535-1-Ig, Proteintech), anti-phospho-NF- κ B p65 (p-NF- κ B p65, 1 : 1000, #3033, Cell Signaling Technology), anti-phospho-I κ B α (phospho-I κ B α , 1 : 1000, #9246, Cell Signaling Technology), and anti-I κ B α (1 : 1000, #4812, Cell Signaling Technology).

    Techniques: Expressing, Western Blot

    Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α (Ser32) from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Cichoric Acid Ameliorates Monosodium Urate-Induced Inflammatory Response by Reducing NLRP3 Inflammasome Activation via Inhibition of NF- k B Signaling Pathway

    doi: 10.1155/2021/8868527

    Figure Lengend Snippet: Relative levels of phosphorylation of multiple proteins in the THP-M cells. The protein levels of p65, IL-1 β , pro-IL-1 β , I κ B α , and NLRP3 and the levels of phosphorylated p65 (Ser536) and p-I κ B α (Ser32) from different groups were analyzed by western blotting (a). The protein levels of phos-p65 (Ser536) (b), phos-I κ B α (Ser32) (c), I κ B α (d), IL-1 β (e), and NLRP3 (f) were normalized to MUS. The results are presented as mean ± SD ( n = 3). ∗∗ p < 0.01 vs. the MSU group.

    Article Snippet: The specifics of the other materials used in the study are as follows: COX-1 Inhibitor Screening Kit (Fluorometric) (BioVision, Inc., Mountain View, CA, USA); COX-2 Inhibitor Screening Kit (Beyotime Biotech Co., Ltd., China); PVDF (0.45 μ m) (Millipore, Schwalbach, Germany); precolor protein marker (Green BioResearch, LA, USA); fuchsia, Tween 20, acrylamide, and sodium dodecyl sulfate (Solon, OH, USA); protein lysate (RIPA) (Beyotime Biotechnology, Shanghai, China); western blotting membrane regeneration solution (C500031, Sangon Biotech, Shanghai, China); fetal bovine serum (FBS) (GIBCO, NY, USA); RPM1-1640 culture medium (GIBCO, NY, USA); trypsin (Gibco, Grand Island, NY, USA); phorbol myristate acetate (PMA) and penicillin (Sigma-Aldrich, St. Louis, MO); TNF- α ELISA kit (DTA00D) and IL-1 β ELISA kit (DLB50) (R&D Systems, Minnesota, USA); anti-CD86 antibody (ab77276), anti-TNF- α antibody (ab269282), anti-NLRP3 antibody (ab263899), PKA kinase activity assay kit (ab139435), Prostaglandin E2 ELISA Kit (ab133021), and cAMP assay kit (Competitive ELISA) (ab234585) (Abcam, Cambridge, UK); anti-IL-1 β antibody (12703), anti-phos-p65 (Ser536) antibody (3033), anti-p65 antibody (8242), anti-phos-I κ B α (Ser32) antibody (2859), anti-I κ B α antibody (9242), anti-COX-2 antibody (12282) (CST, Boston, USA), and TRIzol (Invitrogen); reverse transcription kit (1708843) and SsoFast EvaGreen Supermix (1725200) (Bio-Rad Laboratories, California, USA); primer (Sangon Biotech Co., Ltd. Shanghai, China); BD Cytofix/Cytoperm™ Fixation/Permeabilization Kit (554714) (BD, New Jersey, USA); ionomycin (SQ23377) (MCE, Shanghai, China); MSU (Invivogen, France); Berthold LB941 microporous plate-type multifunctional enzyme labeling instrument; flow cytometry (Beckman DxFLEX, USA); real-time fluorescence quantitative PCR instrument (ABI 7500, ABI, USA).

    Techniques: Western Blot