Journal: Metabolites

Article Title: Glucolipotoxic Stress-Induced Mig6 Desensitizes EGFR Signaling and Promotes Pancreatic Beta Cell Death

doi: 10.3390/metabo13050627

Figure Lengend Snippet: List of antibodies used for immunoblotting.

Article Snippet: Anti-phospho-EGFR (Tyr1068) , Cell Signaling, #3777 , 1:1250.

Techniques: Western Blot

Journal: Cells

Article Title: Molecular Analysis of the Superior Efficacy of a Dual Epidermal Growth Factor Receptor (EGFR)-DNA-Targeting Combi-Molecule in Comparison with Its Putative Prodrugs 6-Mono-Alkylamino- and 6,6-Dialkylaminoquinazoline in a Human Osteosarcoma Xenograft Model

doi: 10.3390/cells12060914

Figure Lengend Snippet: Immunohistochemistry and Western blot analysis of the effects of ZR2002, JS84 and JS61 on tumor tissues. ZR2002 decreases the phosphorylation of EGFR (P-EGFR). ( A ) Representative sections of Saos-2 tumors stained for P-EGFR. ( B ) Western blots for P-EGFR (Tyr 1068) and EGFR, where β-actin was used as internal control. Each band corresponds to different tumor lysates. ( C ) Quantification of Western blot: ratio of band intensity between P-EGFR and EGFR total protein. Data are expressed as means ± SEM. * p < 0.05 between the two groups.

Article Snippet: The membranes were blocked with 5% BSA in PBS 1x with 0.1% Tween-20 and immunoblotted overnight with the following antibodies—P-EGFR (Tyr1068) (D7A5) (1:1000; #3777; Cell Signaling, Danvers, MA, USA), EGFR (1:1000; #2646; Cell Signaling, Danvers, MA, USA) and phospho-histone H2A.X (Ser139) (1:1000; #2577; Cell Signaling, Danvers, MA, USA)—for tumor-tissue-derived blots and immunoblotted with the EGFR antibody for tumor-cell-derived blots.

Techniques: Immunohistochemistry, Western Blot, Staining