ptmscan phospho cdk cdk mapk substrate motif antibodies  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc ptmscan phospho cdk cdk mapk substrate motif antibodies
    Ptmscan Phospho Cdk Cdk Mapk Substrate Motif Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptmscan phospho cdk cdk mapk substrate motif antibodies/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptmscan phospho cdk cdk mapk substrate motif antibodies - by Bioz Stars, 2023-06
    94/100 stars

    Images

    ptmscan phospho cdk cdk mapk substrate motif antibodies  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc ptmscan phospho cdk cdk mapk substrate motif antibodies
    Ptmscan Phospho Cdk Cdk Mapk Substrate Motif Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptmscan phospho cdk cdk mapk substrate motif antibodies/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptmscan phospho cdk cdk mapk substrate motif antibodies - by Bioz Stars, 2023-06
    94/100 stars

    Images

    anti cdk substrate antibody  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti cdk substrate antibody
    Anti Cdk Substrate Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cdk substrate antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cdk substrate antibody - by Bioz Stars, 2023-06
    94/100 stars

    Images

    phospho cdk substrate motif  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc phospho cdk substrate motif
    Phospho Cdk Substrate Motif, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho cdk substrate motif/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho cdk substrate motif - by Bioz Stars, 2023-06
    94/100 stars

    Images

    anti phospho cdk substrate motif k h psp  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti phospho cdk substrate motif k h psp
    Anti Phospho Cdk Substrate Motif K H Psp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho cdk substrate motif k h psp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho cdk substrate motif k h psp - by Bioz Stars, 2023-06
    94/100 stars

    Images

    anti phosphoserine cdks substrate antibody  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti phosphoserine cdks substrate antibody
    Anti Phosphoserine Cdks Substrate Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphoserine cdks substrate antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphoserine cdks substrate antibody - by Bioz Stars, 2023-06
    94/100 stars

    Images

    ptmscan phospho cdk cdk mapk substrate motif antibodies  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc ptmscan phospho cdk cdk mapk substrate motif antibodies
    Ptmscan Phospho Cdk Cdk Mapk Substrate Motif Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptmscan phospho cdk cdk mapk substrate motif antibodies/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptmscan phospho cdk cdk mapk substrate motif antibodies - by Bioz Stars, 2023-06
    94/100 stars

    Images

    k h psp  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc k h psp
    K H Psp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k h psp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k h psp - by Bioz Stars, 2023-06
    94/100 stars

    Images

    p cdk substrate  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc p cdk substrate
    P Cdk Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p cdk substrate/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p cdk substrate - by Bioz Stars, 2023-06
    94/100 stars

    Images

    anti phospho cdk substrate  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc anti phospho cdk substrate
    CDK2‐CycE <t>phosphorylates</t> <t>WRN.</t> (a) FLAG/His‐tagged WRN protein was purified from baculovirus‐infected insect cells. The purified WRN protein was in vitro phosphorylated with the indicated <t>CDK‐Cyc</t> combinations and probed by Western blot for the antigens shown. Phosphorylated serine/threonine blot intensities were quantified by normalizing with WRN and basal phosphorylation without CDKs. Asterisks indicated directly above the columns represent p‐value calculations compared to controls (basal phosphorylation without CDKs). (b) To remove the basal phosphorylation seen in A, purified WRN proteins were treated with λPPase, then immunoprecipitated with FLAG beads and subjected to in vitro phosphorylation with CDK2‐CycA or CDK2‐Cyc2 combinations, followed by Western blot analysis. Phosphorylated serine blot intensity was quantified by normalizing with WRN. Error bars represent standard deviation. p ‐value, *, <0.05; **, <0.01; ***, <0.001
    Anti Phospho Cdk Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho cdk substrate/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho cdk substrate - by Bioz Stars, 2023-06
    94/100 stars

    Images

    1) Product Images from "CDK2 phosphorylation of Werner protein (WRN) contributes to WRN’s DNA double‐strand break repair pathway choice"

    Article Title: CDK2 phosphorylation of Werner protein (WRN) contributes to WRN’s DNA double‐strand break repair pathway choice

    Journal: Aging Cell

    doi: 10.1111/acel.13484

    CDK2‐CycE phosphorylates WRN. (a) FLAG/His‐tagged WRN protein was purified from baculovirus‐infected insect cells. The purified WRN protein was in vitro phosphorylated with the indicated CDK‐Cyc combinations and probed by Western blot for the antigens shown. Phosphorylated serine/threonine blot intensities were quantified by normalizing with WRN and basal phosphorylation without CDKs. Asterisks indicated directly above the columns represent p‐value calculations compared to controls (basal phosphorylation without CDKs). (b) To remove the basal phosphorylation seen in A, purified WRN proteins were treated with λPPase, then immunoprecipitated with FLAG beads and subjected to in vitro phosphorylation with CDK2‐CycA or CDK2‐Cyc2 combinations, followed by Western blot analysis. Phosphorylated serine blot intensity was quantified by normalizing with WRN. Error bars represent standard deviation. p ‐value, *, <0.05; **, <0.01; ***, <0.001
    Figure Legend Snippet: CDK2‐CycE phosphorylates WRN. (a) FLAG/His‐tagged WRN protein was purified from baculovirus‐infected insect cells. The purified WRN protein was in vitro phosphorylated with the indicated CDK‐Cyc combinations and probed by Western blot for the antigens shown. Phosphorylated serine/threonine blot intensities were quantified by normalizing with WRN and basal phosphorylation without CDKs. Asterisks indicated directly above the columns represent p‐value calculations compared to controls (basal phosphorylation without CDKs). (b) To remove the basal phosphorylation seen in A, purified WRN proteins were treated with λPPase, then immunoprecipitated with FLAG beads and subjected to in vitro phosphorylation with CDK2‐CycA or CDK2‐Cyc2 combinations, followed by Western blot analysis. Phosphorylated serine blot intensity was quantified by normalizing with WRN. Error bars represent standard deviation. p ‐value, *, <0.05; **, <0.01; ***, <0.001

    Techniques Used: Purification, Infection, In Vitro, Western Blot, Immunoprecipitation, Standard Deviation

    p cdk1 substrates  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Cell Signaling Technology Inc p cdk1 substrates
    Erianin induces cell cycle arrest at the M phase in PC3 cells. (A, B) PC3 cells were treated with erianin at indicated concentrations for 24 h. (A) Cell cycle phase was determined by flow cytometry with propidium iodide (PI) staining. G 0 /G 1 , S and G 2 /M phase populations were estimated using Flowjo software and are indicated in purple, yellow and green, respectively. (B) Percentages of cells at each phase was quantified by Flowjo software. Data are expressed as mean ± SD. n=5. * p < 0.05; *** p < 0.001, versus untreated control. (C) PC3 cells were treated with erianin at 50 nM for indicated times. Cyclin b1 and phosphorylated <t>CDK1</t> substrates were examined by Western blotting. (D, E) Cells were treated with S-trityl-L-cysteine (STLC) at 10 µM for 16 h to synchronize cells at the prometaphase. Then STLC was withdrawn and cell cycle progression to metaphase, telophase and interphase were monitored in the presence ot absence of erianin at 100 nM for 1 h. Confocal images were captured. Bar = 10 µm (D) . Percentages of cells at each cell cycle phase were quantified (E) .
    P Cdk1 Substrates, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p cdk1 substrates/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p cdk1 substrates - by Bioz Stars, 2023-06
    94/100 stars

    Images

    1) Product Images from "Ceramide Regulates Anti-Tumor Mechanisms of Erianin in Androgen-Sensitive and Castration-Resistant Prostate Cancers"

    Article Title: Ceramide Regulates Anti-Tumor Mechanisms of Erianin in Androgen-Sensitive and Castration-Resistant Prostate Cancers

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2021.738078

    Erianin induces cell cycle arrest at the M phase in PC3 cells. (A, B) PC3 cells were treated with erianin at indicated concentrations for 24 h. (A) Cell cycle phase was determined by flow cytometry with propidium iodide (PI) staining. G 0 /G 1 , S and G 2 /M phase populations were estimated using Flowjo software and are indicated in purple, yellow and green, respectively. (B) Percentages of cells at each phase was quantified by Flowjo software. Data are expressed as mean ± SD. n=5. * p < 0.05; *** p < 0.001, versus untreated control. (C) PC3 cells were treated with erianin at 50 nM for indicated times. Cyclin b1 and phosphorylated CDK1 substrates were examined by Western blotting. (D, E) Cells were treated with S-trityl-L-cysteine (STLC) at 10 µM for 16 h to synchronize cells at the prometaphase. Then STLC was withdrawn and cell cycle progression to metaphase, telophase and interphase were monitored in the presence ot absence of erianin at 100 nM for 1 h. Confocal images were captured. Bar = 10 µm (D) . Percentages of cells at each cell cycle phase were quantified (E) .
    Figure Legend Snippet: Erianin induces cell cycle arrest at the M phase in PC3 cells. (A, B) PC3 cells were treated with erianin at indicated concentrations for 24 h. (A) Cell cycle phase was determined by flow cytometry with propidium iodide (PI) staining. G 0 /G 1 , S and G 2 /M phase populations were estimated using Flowjo software and are indicated in purple, yellow and green, respectively. (B) Percentages of cells at each phase was quantified by Flowjo software. Data are expressed as mean ± SD. n=5. * p < 0.05; *** p < 0.001, versus untreated control. (C) PC3 cells were treated with erianin at 50 nM for indicated times. Cyclin b1 and phosphorylated CDK1 substrates were examined by Western blotting. (D, E) Cells were treated with S-trityl-L-cysteine (STLC) at 10 µM for 16 h to synchronize cells at the prometaphase. Then STLC was withdrawn and cell cycle progression to metaphase, telophase and interphase were monitored in the presence ot absence of erianin at 100 nM for 1 h. Confocal images were captured. Bar = 10 µm (D) . Percentages of cells at each cell cycle phase were quantified (E) .

    Techniques Used: Flow Cytometry, Staining, Software, Western Blot

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Cell Signaling Technology Inc ptmscan phospho cdk cdk mapk substrate motif antibodies
    Ptmscan Phospho Cdk Cdk Mapk Substrate Motif Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptmscan phospho cdk cdk mapk substrate motif antibodies/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ptmscan phospho cdk cdk mapk substrate motif antibodies - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc anti cdk substrate antibody
    Anti Cdk Substrate Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti cdk substrate antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti cdk substrate antibody - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc phospho cdk substrate motif
    Phospho Cdk Substrate Motif, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho cdk substrate motif/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho cdk substrate motif - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc anti phospho cdk substrate motif k h psp
    Anti Phospho Cdk Substrate Motif K H Psp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho cdk substrate motif k h psp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho cdk substrate motif k h psp - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc anti phosphoserine cdks substrate antibody
    Anti Phosphoserine Cdks Substrate Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phosphoserine cdks substrate antibody/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phosphoserine cdks substrate antibody - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc k h psp
    K H Psp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k h psp/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    k h psp - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc p cdk substrate
    P Cdk Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p cdk substrate/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p cdk substrate - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc anti phospho cdk substrate
    CDK2‐CycE <t>phosphorylates</t> <t>WRN.</t> (a) FLAG/His‐tagged WRN protein was purified from baculovirus‐infected insect cells. The purified WRN protein was in vitro phosphorylated with the indicated <t>CDK‐Cyc</t> combinations and probed by Western blot for the antigens shown. Phosphorylated serine/threonine blot intensities were quantified by normalizing with WRN and basal phosphorylation without CDKs. Asterisks indicated directly above the columns represent p‐value calculations compared to controls (basal phosphorylation without CDKs). (b) To remove the basal phosphorylation seen in A, purified WRN proteins were treated with λPPase, then immunoprecipitated with FLAG beads and subjected to in vitro phosphorylation with CDK2‐CycA or CDK2‐Cyc2 combinations, followed by Western blot analysis. Phosphorylated serine blot intensity was quantified by normalizing with WRN. Error bars represent standard deviation. p ‐value, *, <0.05; **, <0.01; ***, <0.001
    Anti Phospho Cdk Substrate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti phospho cdk substrate/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti phospho cdk substrate - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    94
    Cell Signaling Technology Inc p cdk1 substrates
    Erianin induces cell cycle arrest at the M phase in PC3 cells. (A, B) PC3 cells were treated with erianin at indicated concentrations for 24 h. (A) Cell cycle phase was determined by flow cytometry with propidium iodide (PI) staining. G 0 /G 1 , S and G 2 /M phase populations were estimated using Flowjo software and are indicated in purple, yellow and green, respectively. (B) Percentages of cells at each phase was quantified by Flowjo software. Data are expressed as mean ± SD. n=5. * p < 0.05; *** p < 0.001, versus untreated control. (C) PC3 cells were treated with erianin at 50 nM for indicated times. Cyclin b1 and phosphorylated <t>CDK1</t> substrates were examined by Western blotting. (D, E) Cells were treated with S-trityl-L-cysteine (STLC) at 10 µM for 16 h to synchronize cells at the prometaphase. Then STLC was withdrawn and cell cycle progression to metaphase, telophase and interphase were monitored in the presence ot absence of erianin at 100 nM for 1 h. Confocal images were captured. Bar = 10 µm (D) . Percentages of cells at each cell cycle phase were quantified (E) .
    P Cdk1 Substrates, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p cdk1 substrates/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p cdk1 substrates - by Bioz Stars, 2023-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    CDK2‐CycE phosphorylates WRN. (a) FLAG/His‐tagged WRN protein was purified from baculovirus‐infected insect cells. The purified WRN protein was in vitro phosphorylated with the indicated CDK‐Cyc combinations and probed by Western blot for the antigens shown. Phosphorylated serine/threonine blot intensities were quantified by normalizing with WRN and basal phosphorylation without CDKs. Asterisks indicated directly above the columns represent p‐value calculations compared to controls (basal phosphorylation without CDKs). (b) To remove the basal phosphorylation seen in A, purified WRN proteins were treated with λPPase, then immunoprecipitated with FLAG beads and subjected to in vitro phosphorylation with CDK2‐CycA or CDK2‐Cyc2 combinations, followed by Western blot analysis. Phosphorylated serine blot intensity was quantified by normalizing with WRN. Error bars represent standard deviation. p ‐value, *, <0.05; **, <0.01; ***, <0.001

    Journal: Aging Cell

    Article Title: CDK2 phosphorylation of Werner protein (WRN) contributes to WRN’s DNA double‐strand break repair pathway choice

    doi: 10.1111/acel.13484

    Figure Lengend Snippet: CDK2‐CycE phosphorylates WRN. (a) FLAG/His‐tagged WRN protein was purified from baculovirus‐infected insect cells. The purified WRN protein was in vitro phosphorylated with the indicated CDK‐Cyc combinations and probed by Western blot for the antigens shown. Phosphorylated serine/threonine blot intensities were quantified by normalizing with WRN and basal phosphorylation without CDKs. Asterisks indicated directly above the columns represent p‐value calculations compared to controls (basal phosphorylation without CDKs). (b) To remove the basal phosphorylation seen in A, purified WRN proteins were treated with λPPase, then immunoprecipitated with FLAG beads and subjected to in vitro phosphorylation with CDK2‐CycA or CDK2‐Cyc2 combinations, followed by Western blot analysis. Phosphorylated serine blot intensity was quantified by normalizing with WRN. Error bars represent standard deviation. p ‐value, *, <0.05; **, <0.01; ***, <0.001

    Article Snippet: Proteins were resolved in 4 to 15% Mini‐PROTEAN TGX gels (BioRad, Hercules, CA, USA), electroblotted to nitrocellulose membrane, and visualized using antibodies against anti‐WRN (in house), anti‐phospho‐CDK substrate (Cell Signaling, 9477), anti‐CDK1 (Abcam, ab133327), anti‐CDK2 (Abcam, ab32147), anti‐CycA2 (Abcam, ab38), anti‐CycE (Abcam, ab133266), anti‐DNA‐PKcs (BD, 610804), anti‐KU70 (ThermoFisher, PA5‐27538), and anti‐RPA (Calbiochem, NA18).

    Techniques: Purification, Infection, In Vitro, Western Blot, Immunoprecipitation, Standard Deviation

    Erianin induces cell cycle arrest at the M phase in PC3 cells. (A, B) PC3 cells were treated with erianin at indicated concentrations for 24 h. (A) Cell cycle phase was determined by flow cytometry with propidium iodide (PI) staining. G 0 /G 1 , S and G 2 /M phase populations were estimated using Flowjo software and are indicated in purple, yellow and green, respectively. (B) Percentages of cells at each phase was quantified by Flowjo software. Data are expressed as mean ± SD. n=5. * p < 0.05; *** p < 0.001, versus untreated control. (C) PC3 cells were treated with erianin at 50 nM for indicated times. Cyclin b1 and phosphorylated CDK1 substrates were examined by Western blotting. (D, E) Cells were treated with S-trityl-L-cysteine (STLC) at 10 µM for 16 h to synchronize cells at the prometaphase. Then STLC was withdrawn and cell cycle progression to metaphase, telophase and interphase were monitored in the presence ot absence of erianin at 100 nM for 1 h. Confocal images were captured. Bar = 10 µm (D) . Percentages of cells at each cell cycle phase were quantified (E) .

    Journal: Frontiers in Oncology

    Article Title: Ceramide Regulates Anti-Tumor Mechanisms of Erianin in Androgen-Sensitive and Castration-Resistant Prostate Cancers

    doi: 10.3389/fonc.2021.738078

    Figure Lengend Snippet: Erianin induces cell cycle arrest at the M phase in PC3 cells. (A, B) PC3 cells were treated with erianin at indicated concentrations for 24 h. (A) Cell cycle phase was determined by flow cytometry with propidium iodide (PI) staining. G 0 /G 1 , S and G 2 /M phase populations were estimated using Flowjo software and are indicated in purple, yellow and green, respectively. (B) Percentages of cells at each phase was quantified by Flowjo software. Data are expressed as mean ± SD. n=5. * p < 0.05; *** p < 0.001, versus untreated control. (C) PC3 cells were treated with erianin at 50 nM for indicated times. Cyclin b1 and phosphorylated CDK1 substrates were examined by Western blotting. (D, E) Cells were treated with S-trityl-L-cysteine (STLC) at 10 µM for 16 h to synchronize cells at the prometaphase. Then STLC was withdrawn and cell cycle progression to metaphase, telophase and interphase were monitored in the presence ot absence of erianin at 100 nM for 1 h. Confocal images were captured. Bar = 10 µm (D) . Percentages of cells at each cell cycle phase were quantified (E) .

    Article Snippet: Immunoblot analyses were conducted according to standard protocol with the following antisera: caspase-3 (#9662), PARP (#9542), β-actin (#3770), IRE1α (#3294), p-eIF2α (#3597), t-eIF2α (#2103), p-P38 MAPK (#4511), t-P38 MAPK (#8690), p-JNK1/2 (#4668), t-JNK1/2 (#9252), p-ERK1/2 (#9101), t-ERK1/2 (#9102), CHOP (#5554), Bim (#2933), Bcl-2 (#4223), Bax (#5023), p-Akt (#4060), t-Akt (#9272), GAPDH (#5174), cyclin b1 (#12231), p-CDK1 substrates (#9477) and FLAG (#14793) from Cell Signaling Technology; p62 (#ab56416) and Mcl-1 (#ab31948) antibodies were purchased from Abcam, while LC3 antibody (#NB100-2220) was obtained from Novus Biologicals.

    Techniques: Flow Cytometry, Staining, Software, Western Blot