Journal: Advanced Science

Article Title: ERK Inhibition Promotes Engraftment of Allografts by Reprogramming T‐Cell Metabolism

doi: 10.1002/advs.202206768

Figure Lengend Snippet: Antibodies and dyes used for western blotting and flow cytometry

Article Snippet: Phospho‐c‐Fos (Ser32) (D82C12) XP Rabbit mAb (Alexa Fluor 488 Conjugate) , CST , cat#:8709S; RRID: AB_11179220.

Techniques: Western Blot

Journal: Oncology Letters

Article Title: Hesperidin inhibits tobacco smoke-induced pulmonary cell proliferation and EMT in mouse lung tissues via the p38 signaling pathway

doi: 10.3892/ol.2022.13616

Figure Lengend Snippet: TS increases p38 pathway activation in mouse lung tissues. Mice were exposed to TS or FA for 6 h daily for a total of 12 weeks. (A) Western blotting was used to detect the effect of 12 weeks of TS exposure on the expression of p38, c-Fos, p-p38 and p-c-Fos. (B) Evaluation of the phosphorylated protein/total protein ratio by density analysis of western blotting. **P<0.01 compared with FA. The error bars shown in the graphs indicate the standard deviation. FA, filtered air; p-, phosphorylated; TS, tobacco smoke.

Article Snippet: Phosphorylated p38 (catalogue number, 4511T; 1:500), phosphorylated c-Fos (catalogue number, 5348T; 1:1,000), p38 (catalogue number, 8690T; 1:1,000), c-Fos (catalogue number, 2250T; 1:1,000), E-cadherin (catalogue number, 3195T; 1:1,000) and N-cadherin (catalogue number, 13116T; 1:1,000) antibodies were purchased from Cell Signaling Technology, Inc. Vimentin (catalogue number, MB65651; 1:1,000), proliferating cell nuclear antigen (PCNA) (catalogue number, MB0156; 1:500) and GAPDH (catalogue number, BS65483M; 1:2,000) antibodies were purchased from Bioworld Technology, Inc. Horseradish peroxidase-conjugated secondary antibodies were purchased from Invitrogen; Thermo Fisher Scientific, Inc. (catalogue numbers, 31430 and 31460; 1:2,000).

Techniques: Activation Assay, Western Blot, Expressing, Standard Deviation

Journal: Oncology Letters

Article Title: Hesperidin inhibits tobacco smoke-induced pulmonary cell proliferation and EMT in mouse lung tissues via the p38 signaling pathway

doi: 10.3892/ol.2022.13616

Figure Lengend Snippet: SB203580 inhibits TS-induced p38 pathway activation. Mice were treated with FA, TS, TS + DMSO or TS + SB203580 for 12 weeks. (A) Western blot analysis revealed that SB203580 inhibited the TS-induced changes of p-p38 and p-c-Fos. (B) Evaluation of the phosphorylated protein/total protein ratio by density analysis of western blotting. **P<0.01, compared with the FA control; ## P<0.01, compared with TS alone. The error bars shown in the graphs indicate the standard deviation. FA, filtered air; p-, phosphorylated; TS, tobacco smoke.

Article Snippet: Phosphorylated p38 (catalogue number, 4511T; 1:500), phosphorylated c-Fos (catalogue number, 5348T; 1:1,000), p38 (catalogue number, 8690T; 1:1,000), c-Fos (catalogue number, 2250T; 1:1,000), E-cadherin (catalogue number, 3195T; 1:1,000) and N-cadherin (catalogue number, 13116T; 1:1,000) antibodies were purchased from Cell Signaling Technology, Inc. Vimentin (catalogue number, MB65651; 1:1,000), proliferating cell nuclear antigen (PCNA) (catalogue number, MB0156; 1:500) and GAPDH (catalogue number, BS65483M; 1:2,000) antibodies were purchased from Bioworld Technology, Inc. Horseradish peroxidase-conjugated secondary antibodies were purchased from Invitrogen; Thermo Fisher Scientific, Inc. (catalogue numbers, 31430 and 31460; 1:2,000).

Techniques: Activation Assay, Western Blot, Standard Deviation

Journal: Oncology Letters

Article Title: Hesperidin inhibits tobacco smoke-induced pulmonary cell proliferation and EMT in mouse lung tissues via the p38 signaling pathway

doi: 10.3892/ol.2022.13616

Figure Lengend Snippet: Hesperidin attenuates activation of the p38 pathway by TS. Mice were treated with FA, TS, TS + DMSO and TS + hesperidin for 12 weeks. (A) Western blotting was used to analyze the expression changes of p38, c-Fos, p-p38 and p-c-Fos. (B) Evaluation of the phosphorylated protein/total protein ratio by density analysis of western blotting. **P<0.01, compared with FA; ## P<0.01, compared with TS only. The error bars shown in the graphs indicate the standard deviation. FA, filtered air; p-, phosphorylated; TS, tobacco smoke.

Article Snippet: Phosphorylated p38 (catalogue number, 4511T; 1:500), phosphorylated c-Fos (catalogue number, 5348T; 1:1,000), p38 (catalogue number, 8690T; 1:1,000), c-Fos (catalogue number, 2250T; 1:1,000), E-cadherin (catalogue number, 3195T; 1:1,000) and N-cadherin (catalogue number, 13116T; 1:1,000) antibodies were purchased from Cell Signaling Technology, Inc. Vimentin (catalogue number, MB65651; 1:1,000), proliferating cell nuclear antigen (PCNA) (catalogue number, MB0156; 1:500) and GAPDH (catalogue number, BS65483M; 1:2,000) antibodies were purchased from Bioworld Technology, Inc. Horseradish peroxidase-conjugated secondary antibodies were purchased from Invitrogen; Thermo Fisher Scientific, Inc. (catalogue numbers, 31430 and 31460; 1:2,000).

Techniques: Activation Assay, Western Blot, Expressing, Standard Deviation