Journal: Physiological Reports

Article Title: Decreased AMP‐activated protein kinase (AMPK) function and protective effect of metformin in neonatal rat pups exposed to hyperoxia lung injury

doi: 10.14814/phy2.14587

Figure Lengend Snippet: Representative immunoblots for phos‐ Thr172 AMPK‐α1 from the lung homogenates obtained for the four groups of rat pups at postnatal day 4 (P4), P10 and P21. Summary data are shown in bar graphs as mean ± SD for n = 4 at P4 and n = 8 at P10 and P21. * Indicates p < .05 by ANOVA and Tukey's post‐hoc test from normoxia + saline and # from hyperoxia + saline groups. Hyperoxia decreased the p‐AMPK protein level at P10 and P21 lung samples. P‐AMPK protein levels increased in metformin‐treated hyperoxia group at all three ages, P4, P10, and P21. HOX, hyperoxia; NOX, normoxia

Article Snippet: For immunoblotting, the following reagents were used: Rabbit Anti‐phos‐ Thr172 AMPK‐α1 polyclonal Ab and mouse Anti‐p‐ Ser1177 eNOS Monoclonal Ab were from Cell Signaling Technology.

Techniques: Western Blot

Journal: Endocrinology

Article Title: Bis-Indole–Derived NR4A1 Ligands and Metformin Exhibit NR4A1-Dependent Glucose Metabolism and Uptake in C2C12 Cells

doi: 10.1210/en.2017-03049

Figure Lengend Snippet: Primary Antibodies

Article Snippet: Mouse myoblasts C2C12 cells were obtained from American Type Culture Collection (Manassas, VA) and were maintained in Dulbecco’s modified Eagle medium supplemented with 10% fetal bovine serum and antibiotics at 37°C in an incubator with 5% CO 2 differentiation induced by switching to differentiation medium with Dulbecco’s modified Eagle medium containing 2% horse serum for 5 days. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antigen Source Species Dilution Manufacturer; Catalog No.; RRID NR4A1/Nur77 Rabbit 1:1000 Abcam; ab109180; AB_10861258 Rab4 Rabbit 1:1000 Cell Signaling Technology; 2167S; AB_2253579 p-AMPK Rabbit 1:1000 Cell Signaling Technology; 2535; AB_331250 AMPK Rabbit 1:1000 Cell Signaling Technology; 5831; AB_10622186 GLUT4 Mouse 1:1000 Cell Signaling Technology; 2213; AB_823508 Monoclonal anti-flag M2 Mouse 1:1000 Sigma-Aldrich; F1804; AB_262044 β -Actin Mouse 1:10,000 Sigma-Aldrich; A1978; AB_476692 Open in a separate window Abbreviation: RRID, Research Resource Identifier.

Techniques:

Journal: Endocrinology

Article Title: Developmental Programming: Impact of Gestational Steroid and Metabolic Milieus on Mediators of Insulin Sensitivity in Prenatal Testosterone–Treated Female Sheep

doi: 10.1210/en.2017-00460

Figure Lengend Snippet: Antibodies Used

Article Snippet: Band densities were determined using ImageJ software. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used RRID Bovine Adiponectin Bovine antiadiponectin Dr. Helga Sauerwein (University of Bonn, Germany) Rabbit; polyclonal WB 1:1K AB_2650604 p-AMPK Phospho-AMPK α (Thr172) (D79.5E) Cell Signaling, 4182 Rabbit; monoclonal WB 1:1K AB_2169396 AMPK AMPKα (D63G4) Cell Signaling, 5832 Rabbit; monoclonal WB 1:1K AB_10624867 GAPDH GAPDH (14C10) Cell Signaling, 3683 Rabbit; monoclonal WB 1:1K AB_1642205 Open in a separate window Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; RRID, research resource identifier; WB, Western blot.

Techniques: Sequencing