phosphorylated (Cell Signaling Technology Inc)


Structured Review
Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
phosphorylated (Cell Signaling Technology Inc)


Structured Review
Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti p akt (Cell Signaling Technology Inc)


Structured Review
Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti p akt/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
p akt (Cell Signaling Technology Inc)


Structured Review
P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p akt/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
phospho protein kinase b ser473 (Cell Signaling Technology Inc)


Structured Review
Phospho Protein Kinase B Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho protein kinase b ser473/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rabbit monoclonal anti phospho akt ser473 (Cell Signaling Technology Inc)


Structured Review
Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
d9e xp 4060 (Cell Signaling Technology Inc)


Structured Review
D9e Xp 4060, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/d9e xp 4060/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
phospho akt ser473 rabbit mab (Cell Signaling Technology Inc)


Structured Review
Phospho Akt Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho akt ser473 rabbit mab/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
phospho akt ser473 (Cell Signaling Technology Inc)


Structured Review
Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti ps473 akt (Cell Signaling Technology Inc)


Structured Review
Anti Ps473 Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti ps473 akt/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
anti pt246 pras40 (Cell Signaling Technology Inc)


Structured Review

Anti Pt246 Pras40, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti pt246 pras40/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "B cell–intrinsic requirement for WNK1 kinase in antibody responses in mice"
Article Title: B cell–intrinsic requirement for WNK1 kinase in antibody responses in mice
Journal: The Journal of Experimental Medicine
doi: 10.1084/jem.20211827

Figure Legend Snippet: Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated PRAS40 (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .
Techniques Used: Western Blot, Expressing, MANN-WHITNEY

Figure Legend Snippet: WNK1 is not required for c-MYC upregulation and is not activated by PI3K, AKT, IKK2, or MEK downstream of CD40. (A and B) Immunoblot analysis (top) of cell lysates from control or WNK1-deficient B cells stimulated with anti-IgM (A) or CD40L (B) for the indicated times, probed with antibodies to MYC or α-TUBULIN. Graphs (below) show mean ± SEM levels of MYC normalized to the abundance of α-TUBULIN in each lane. (C) Immunoblot analysis (top) of cell lysates from WT mouse B cells stimulated with LPS for the indicated times, probed with antibodies to p-OXSR1 and α-TUBULIN. Graph shows mean ± SEM levels of p-OXSR1 normalized to the abundance of α-TUBULIN in each lane. (D and E) Top: Immunoblots of total cell lysates from WT mouse B cells treated with vehicle (DMSO), a PI3K inhibitor (PI-103), or an AKT inhibitor (MK2206; D), an IKK2 inhibitor (BI605906) or a MEK1 and MEK2 inhibitor (PD0325901; E) and stimulated for the indicated times with CD40L, probed with antibodies to p-OXSR1, p-AKT, p-PRAS40, p-p105, p-ERK2, or ERK2. Below: graphs of mean ± SEM abundance of p-OXSR1, p-AKT, p-PRAS40, p-p105, and pERK2 in the lanes above, normalized to ERK2. Two-way ANOVA (A, B, D, and E); Mann–Whitney test (C); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: six (A, B, D, and E); and five (C). Data are pooled from two (A and B) or three (C–E) independent experiments. Source data are available for this figure: .
Techniques Used: Western Blot, MANN-WHITNEY