phosphorylated  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc phosphorylated
    Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated - by Bioz Stars, 2023-03
    97/100 stars

    Images

    phosphorylated  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc phosphorylated
    Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated - by Bioz Stars, 2023-03
    97/100 stars

    Images

    anti p akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc anti p akt
    Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p akt/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p akt - by Bioz Stars, 2023-03
    97/100 stars

    Images

    p akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc p akt
    P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p akt/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p akt - by Bioz Stars, 2023-03
    97/100 stars

    Images

    phospho protein kinase b ser473  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc phospho protein kinase b ser473
    Phospho Protein Kinase B Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho protein kinase b ser473/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho protein kinase b ser473 - by Bioz Stars, 2023-03
    96/100 stars

    Images

    rabbit monoclonal anti phospho akt ser473  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt ser473
    Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho akt ser473 - by Bioz Stars, 2023-03
    97/100 stars

    Images

    d9e xp 4060  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Cell Signaling Technology Inc d9e xp 4060
    D9e Xp 4060, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d9e xp 4060/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d9e xp 4060 - by Bioz Stars, 2023-03
    97/100 stars

    Images

    phospho akt ser473 rabbit mab  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho akt ser473 rabbit mab
    Phospho Akt Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 rabbit mab - by Bioz Stars, 2023-03
    86/100 stars

    Images

    phospho akt ser473  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 - by Bioz Stars, 2023-03
    86/100 stars

    Images

    anti ps473 akt  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc anti ps473 akt
    Anti Ps473 Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ps473 akt/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ps473 akt - by Bioz Stars, 2023-03
    96/100 stars

    Images

    anti pt246 pras40  (Cell Signaling Technology Inc)


    Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Cell Signaling Technology Inc anti pt246 pras40
    Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated <t>PRAS40</t> (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .
    Anti Pt246 Pras40, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pt246 pras40/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pt246 pras40 - by Bioz Stars, 2023-03
    96/100 stars

    Images

    1) Product Images from "B cell–intrinsic requirement for WNK1 kinase in antibody responses in mice"

    Article Title: B cell–intrinsic requirement for WNK1 kinase in antibody responses in mice

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20211827

    Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated PRAS40 (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .
    Figure Legend Snippet: Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated PRAS40 (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .

    Techniques Used: Western Blot, Expressing, MANN-WHITNEY

    WNK1 is not required for c-MYC upregulation and is not activated by PI3K, AKT, IKK2, or MEK downstream of CD40. (A and B) Immunoblot analysis (top) of cell lysates from control or WNK1-deficient B cells stimulated with anti-IgM (A) or CD40L (B) for the indicated times, probed with antibodies to MYC or α-TUBULIN. Graphs (below) show mean ± SEM levels of MYC normalized to the abundance of α-TUBULIN in each lane. (C) Immunoblot analysis (top) of cell lysates from WT mouse B cells stimulated with LPS for the indicated times, probed with antibodies to p-OXSR1 and α-TUBULIN. Graph shows mean ± SEM levels of p-OXSR1 normalized to the abundance of α-TUBULIN in each lane. (D and E) Top: Immunoblots of total cell lysates from WT mouse B cells treated with vehicle (DMSO), a PI3K inhibitor (PI-103), or an AKT inhibitor (MK2206; D), an IKK2 inhibitor (BI605906) or a MEK1 and MEK2 inhibitor (PD0325901; E) and stimulated for the indicated times with CD40L, probed with antibodies to p-OXSR1, p-AKT, p-PRAS40, p-p105, p-ERK2, or ERK2. Below: graphs of mean ± SEM abundance of p-OXSR1, p-AKT, p-PRAS40, p-p105, and pERK2 in the lanes above, normalized to ERK2. Two-way ANOVA (A, B, D, and E); Mann–Whitney test (C); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: six (A, B, D, and E); and five (C). Data are pooled from two (A and B) or three (C–E) independent experiments. Source data are available for this figure: .
    Figure Legend Snippet: WNK1 is not required for c-MYC upregulation and is not activated by PI3K, AKT, IKK2, or MEK downstream of CD40. (A and B) Immunoblot analysis (top) of cell lysates from control or WNK1-deficient B cells stimulated with anti-IgM (A) or CD40L (B) for the indicated times, probed with antibodies to MYC or α-TUBULIN. Graphs (below) show mean ± SEM levels of MYC normalized to the abundance of α-TUBULIN in each lane. (C) Immunoblot analysis (top) of cell lysates from WT mouse B cells stimulated with LPS for the indicated times, probed with antibodies to p-OXSR1 and α-TUBULIN. Graph shows mean ± SEM levels of p-OXSR1 normalized to the abundance of α-TUBULIN in each lane. (D and E) Top: Immunoblots of total cell lysates from WT mouse B cells treated with vehicle (DMSO), a PI3K inhibitor (PI-103), or an AKT inhibitor (MK2206; D), an IKK2 inhibitor (BI605906) or a MEK1 and MEK2 inhibitor (PD0325901; E) and stimulated for the indicated times with CD40L, probed with antibodies to p-OXSR1, p-AKT, p-PRAS40, p-p105, p-ERK2, or ERK2. Below: graphs of mean ± SEM abundance of p-OXSR1, p-AKT, p-PRAS40, p-p105, and pERK2 in the lanes above, normalized to ERK2. Two-way ANOVA (A, B, D, and E); Mann–Whitney test (C); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: six (A, B, D, and E); and five (C). Data are pooled from two (A and B) or three (C–E) independent experiments. Source data are available for this figure: .

    Techniques Used: Western Blot, MANN-WHITNEY

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Cell Signaling Technology Inc phosphorylated
    Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phosphorylated - by Bioz Stars, 2023-03
    97/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc anti p akt
    Anti P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti p akt/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti p akt - by Bioz Stars, 2023-03
    97/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc p akt
    P Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p akt/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p akt - by Bioz Stars, 2023-03
    97/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc phospho protein kinase b ser473
    Phospho Protein Kinase B Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho protein kinase b ser473/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho protein kinase b ser473 - by Bioz Stars, 2023-03
    96/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc rabbit monoclonal anti phospho akt ser473
    Rabbit Monoclonal Anti Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal anti phospho akt ser473/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal anti phospho akt ser473 - by Bioz Stars, 2023-03
    97/100 stars
      Buy from Supplier

    97
    Cell Signaling Technology Inc d9e xp 4060
    D9e Xp 4060, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d9e xp 4060/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    d9e xp 4060 - by Bioz Stars, 2023-03
    97/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc phospho akt ser473 rabbit mab
    Phospho Akt Ser473 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473 rabbit mab/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 rabbit mab - by Bioz Stars, 2023-03
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc phospho akt ser473
    Phospho Akt Ser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phospho akt ser473/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    phospho akt ser473 - by Bioz Stars, 2023-03
    86/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc anti ps473 akt
    Anti Ps473 Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ps473 akt/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti ps473 akt - by Bioz Stars, 2023-03
    96/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc anti pt246 pras40
    Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated <t>PRAS40</t> (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .
    Anti Pt246 Pras40, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pt246 pras40/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pt246 pras40 - by Bioz Stars, 2023-03
    96/100 stars
      Buy from Supplier

    Image Search Results


    Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated PRAS40 (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .

    Journal: The Journal of Experimental Medicine

    Article Title: B cell–intrinsic requirement for WNK1 kinase in antibody responses in mice

    doi: 10.1084/jem.20211827

    Figure Lengend Snippet: Signaling from the BCR and CXCR5 activates WNK1 via PI3K and AKT. (A–J) Top: Immunoblots of cell lysates from mouse B cells stimulated for the indicated times with anti-IgM (A–D and I) or CXCL13 (E–H and J) using WT (A and E), WNK1-deficient or control B cells (B and F), B cells expressing kinase-inactive WNK1-D368A or control B cells (C and G), or WT B cells treated with vehicle (DMSO), an inhibitor of WNK family kinases (WNK463; D and H), a PI3K inhibitor (PI-103) or an AKT inhibitor (MK2206; I and J), probed with antibodies to phosphorylated OXSR1 (p-OXSR1), α-TUBULIN, phosphorylated AKT (p-AKT), phosphorylated PRAS40 (p-PRAS40) or ERK2. Bottom: Mean ± SEM abundance of p-OXSR1, p-AKT, and p-PRAS40 in the lanes above, normalized to α-TUBULIN or ERK2. Mann–Whitney test (A and E), two-way ANOVA (B–D, F–H, I, and J); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: four (B, E, and J); five (C, F, G, and I); seven (D and H); and eight (A). Data are pooled from two (B, C, E, F, G, I, and J) or three (A, D, and H) independent experiments. Source data are available for this figure: .

    Article Snippet: The following antibodies and reagents were used for the detection of proteins by immunoblotting: anti-pS325-OXSR1/pS383-STK39 (MRC-PPU), anti-OXSR1 (MRC-PPU) anti-α-TUBULIN (TAT-1, Cell Services STP, The Francis Crick Institute), anti-pS473-AKT (193H12; Cell Signaling Technology), anti-pT246-PRAS40 (C77D7; Cell Signaling Technology), anti-ERK2 (D-2; Santa Cruz Biotechnology), anti-SYK (5F5; BioLegend), anti-pS932-p105 (18E6; Cell Signaling Technology), anti-pT202/pT204-ERK1/2 (197G2; Cell Signaling Technology), and anti-MYC (Y69; Abcam).

    Techniques: Western Blot, Expressing, MANN-WHITNEY

    WNK1 is not required for c-MYC upregulation and is not activated by PI3K, AKT, IKK2, or MEK downstream of CD40. (A and B) Immunoblot analysis (top) of cell lysates from control or WNK1-deficient B cells stimulated with anti-IgM (A) or CD40L (B) for the indicated times, probed with antibodies to MYC or α-TUBULIN. Graphs (below) show mean ± SEM levels of MYC normalized to the abundance of α-TUBULIN in each lane. (C) Immunoblot analysis (top) of cell lysates from WT mouse B cells stimulated with LPS for the indicated times, probed with antibodies to p-OXSR1 and α-TUBULIN. Graph shows mean ± SEM levels of p-OXSR1 normalized to the abundance of α-TUBULIN in each lane. (D and E) Top: Immunoblots of total cell lysates from WT mouse B cells treated with vehicle (DMSO), a PI3K inhibitor (PI-103), or an AKT inhibitor (MK2206; D), an IKK2 inhibitor (BI605906) or a MEK1 and MEK2 inhibitor (PD0325901; E) and stimulated for the indicated times with CD40L, probed with antibodies to p-OXSR1, p-AKT, p-PRAS40, p-p105, p-ERK2, or ERK2. Below: graphs of mean ± SEM abundance of p-OXSR1, p-AKT, p-PRAS40, p-p105, and pERK2 in the lanes above, normalized to ERK2. Two-way ANOVA (A, B, D, and E); Mann–Whitney test (C); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: six (A, B, D, and E); and five (C). Data are pooled from two (A and B) or three (C–E) independent experiments. Source data are available for this figure: .

    Journal: The Journal of Experimental Medicine

    Article Title: B cell–intrinsic requirement for WNK1 kinase in antibody responses in mice

    doi: 10.1084/jem.20211827

    Figure Lengend Snippet: WNK1 is not required for c-MYC upregulation and is not activated by PI3K, AKT, IKK2, or MEK downstream of CD40. (A and B) Immunoblot analysis (top) of cell lysates from control or WNK1-deficient B cells stimulated with anti-IgM (A) or CD40L (B) for the indicated times, probed with antibodies to MYC or α-TUBULIN. Graphs (below) show mean ± SEM levels of MYC normalized to the abundance of α-TUBULIN in each lane. (C) Immunoblot analysis (top) of cell lysates from WT mouse B cells stimulated with LPS for the indicated times, probed with antibodies to p-OXSR1 and α-TUBULIN. Graph shows mean ± SEM levels of p-OXSR1 normalized to the abundance of α-TUBULIN in each lane. (D and E) Top: Immunoblots of total cell lysates from WT mouse B cells treated with vehicle (DMSO), a PI3K inhibitor (PI-103), or an AKT inhibitor (MK2206; D), an IKK2 inhibitor (BI605906) or a MEK1 and MEK2 inhibitor (PD0325901; E) and stimulated for the indicated times with CD40L, probed with antibodies to p-OXSR1, p-AKT, p-PRAS40, p-p105, p-ERK2, or ERK2. Below: graphs of mean ± SEM abundance of p-OXSR1, p-AKT, p-PRAS40, p-p105, and pERK2 in the lanes above, normalized to ERK2. Two-way ANOVA (A, B, D, and E); Mann–Whitney test (C); *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, 0.0001 < P < 0.001; ****, P < 0.0001. Sample sizes: six (A, B, D, and E); and five (C). Data are pooled from two (A and B) or three (C–E) independent experiments. Source data are available for this figure: .

    Article Snippet: The following antibodies and reagents were used for the detection of proteins by immunoblotting: anti-pS325-OXSR1/pS383-STK39 (MRC-PPU), anti-OXSR1 (MRC-PPU) anti-α-TUBULIN (TAT-1, Cell Services STP, The Francis Crick Institute), anti-pS473-AKT (193H12; Cell Signaling Technology), anti-pT246-PRAS40 (C77D7; Cell Signaling Technology), anti-ERK2 (D-2; Santa Cruz Biotechnology), anti-SYK (5F5; BioLegend), anti-pS932-p105 (18E6; Cell Signaling Technology), anti-pT202/pT204-ERK1/2 (197G2; Cell Signaling Technology), and anti-MYC (Y69; Abcam).

    Techniques: Western Blot, MANN-WHITNEY